DNA allelic variations at the loci putatively implicated in seed oil formation among Brassica oilseed cultivars

Molecular Breeding - Tập 26 Số 1 - Trang 51-64 - 2010
Shi, Congguang1, Zhu, Yana1, Li, Yuanlong1, Guo, Wanli1, Chen, Kunming1, Shamsi, Imran Haider1, Hua, Shuijin1, Zhong, Zhaofei1, Zhou, Weijun1, Jiang, Lixi1
1Institute of Crop Science, Zhejiang University, Hangzhou, People’s Republic of China

Tóm tắt

Knowledge about the genes implicated in lipid biosynthesis acquired from the model plant Arabidopsis is useful in understanding the formation of seed oil in Brassica oilseeds. In this paper, we report the screening of polymorphic markers at the loci putative for the seed oil formation between two geographically different genotypes: the Chinese cultivar Ningyou-7 and the European cultivar Tapidor. These primer pairs (150) were designed based on 75 Brassica genes that were Arabidopsis orthologues implicated in the oil formation. A total of 52 out of the 150 primer pairs associated with 47 of the 75 genes showed polymorphisms between the two genotypes. The type of polymorphisms that could be detected on capillary electrophoresis images and their respective visual futures are described. Further, we selected 34 polymorphic markers to scan allelic variations and found rich DNA polymorphisms among the 54 Brassica oilseed cultivars. On the average, each primer pair resulted in 5.6 alleles at the region that was covered. The correlation between the alleles and seed quality traits revealed that the alleles of BnFAD7 were related to the variation of linolenic acid (C18:3) contents among the cultivars. The allele FAD7-ics11170 (3/4)-b that was significantly correlated with high linolenic acid content can be used as an efficient marker for the selection of breeding materials with high linolenic acid content.

Tài liệu tham khảo

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