Explants of Ri-transformed hairy roots of spinach can develop embryogenic calli in the absence of gibberellic acid, an essential growth regulator for induction of embryogenesis from non-transformed roots

Chilton, 1982, Agrobacterium rhizogenes inserts T-DNA into the genomes of the host plant root cells, Nature, 295, 432, 10.1038/295432a0 Simpson, 1986, A disarmed binary vector from Agrobacterium tumefaciens functions in Agrobacterium rhizogenes, Plant Mol. Biol., 6, 403, 10.1007/BF00027133 Tepfer, 1984, Transformation of several species of higher plants by Agrobacterium rhizogenes: sexual transmission of the transformed genotype and phenotype, Cell, 37, 959, 10.1016/0092-8674(84)90430-6 Shen, 1988, Hairy roots are more sensitive to auxin than normal roots, Proc. Natl. Acad. Sci. USA, 85, 3417, 10.1073/pnas.85.10.3417 Nilsson, 1993, Hormonal characterization of transgenic tobacco plants expressing the rol C gene of Agrobacterium rhizogenes TL-DNA, Plant Physiol., 102, 363, 10.1104/pp.102.2.363 Maurel, 1994, Alterations of auxin perception in rol B-transformed tobacco protoplasts, Plant Physiol., 105, 1209, 10.1104/pp.105.4.1209 Moritz, 1998, The gibberellin content of rol A transgenic tobacco plants is specifically altered, J. Plant Physiol., 153, 774, 10.1016/S0176-1617(98)80234-4 Ermayanti, 1994, Stimulation of synthesis and release of swainosonine from transformed roots of Swainsona galegifolia, Phytochemistry, 36, 313, 10.1016/S0031-9422(00)97067-5 Mano, 1986, Production of tropane alkaloids by hairy root cultures of Scopolia japonica, Agric. Biol. Chem., 50, 2715 Sim, 1994, Shikonin production by extractive cultivation in transformed-suspension and hairy root cultures of Lithospermum erythrorhizon, Ann. New York Acad. Sci., 745, 442, 10.1111/j.1749-6632.1994.tb44395.x Knoll, 1997, Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation, Plant Cell Rep., 17, 96, 10.1007/s002990050359 Yang, 1997, Transgenic spinach plants expressing the coat protein of cucumber mosaic virus, In Vitro Cell Dev. Biol. Plant, 33, 200, 10.1007/s11627-997-0022-x Zhang, 1999, An efficient Agrobacterium tumefaciens-mediated transformation and regeneration system for cotyledons of spinach (Spinacia oleracea L.), Plant Cell Rep., 18, 640, 10.1007/s002990050635 Xiao, 1993, Embryogenesis and plant regeneration of spinach (Spinacia oleracea L.) from hypocotyl segments, Plant Cell Rep., 13, 69, 10.1007/BF00235292 Komai, 1996, Effective combinations of plant growth regulators for somatic embryogenesis from spinach root segments, J. Jpn. Soc. Hort. Sci., 65, 559, 10.2503/jjshs.65.559 Komai, 1996, Somatic embryogenesis and plant regeneration in culture of root segments of spinach (Spinacia oleracea L.), Plant Sci., 113, 203, 10.1016/0168-9452(95)04285-7 Komai, 1996, Improvement on the efficiency of somatic embryogenesis from spinach root tissues by applying various sugars, J. Jpn. Soc. Hort. Sci., 65, 67, 10.2503/jjshs.65.67 Ishizaki, 2000, Exogenous ethylene enhances formation of embryogenic callus and inhibits embryogenesis in cultures of explants of spinach roots, J. Am. Soc. Hort. Sci., 125, 21, 10.21273/JASHS.125.1.21 Ishizaki, 2002, Accumulation of a 31-kDa glycoprotein in association with the expression of embryogenic potential by spinach callus in culture, Physiol. Plant, 114, 109, 10.1034/j.1399-3054.2002.1140115.x Murashige, 1962, A revised medium for rapid growth and bio assays with tobacco tissue cultures, Physiol. Plant, 15, 473, 10.1111/j.1399-3054.1962.tb08052.x Daimon, 1990, Hairy root formation in peanut by the wild type strains of Agrobacterium rhizogenes, Plant Tissue Culture Lett., 7, 31, 10.5511/plantbiotechnology1984.7.31 Vervliet, 1975, Characterization of different plaque-forming and defective temperate phages in Agrobacterium strains, J. Gen. Virol., 26, 33, 10.1099/0022-1317-26-1-33 Dellaporta, 1983, A plant DNA minipreparation: version II, Plant Mol. Biol. Rep., 1, 19, 10.1007/BF02712670 Birnboim, 1979, A rapid alkaline extraction procedure for screening recombinant plasmid DNA, Nucleic Acids Res., 7, 1513, 10.1093/nar/7.6.1513 Kiyokawa, 1992, Detection of rol genes of Ri plasmids by PCR method and its application to confirmation of transformation, Plant Tissue Culture Lett., 9, 94, 10.5511/plantbiotechnology1984.9.94 O'Farrell, 1977, High resolution two-dimensional electrophoresis of basic as well as acidic proteins, Cell, 12, 1133, 10.1016/0092-8674(77)90176-3 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Dudits, 1991, Molecular and cellular approaches to the analysis of plant embryo development from somatic cells in vitro, J. Cell Sci., 99, 473, 10.1242/jcs.99.3.473 Stirpe, 1986, Ribosome-inactivating proteins up to date, FEBS Lett., 195, 1, 10.1016/0014-5793(86)80118-1 Savary, 1994, Biosynthesis of defense-related proteins in transformed root cultures of Trichosanthes kirilowii Maxim. var japonicum (Kitam.), Plant Physiol., 106, 1195, 10.1104/pp.106.3.1195 Remi Shih, 1998, A novel type-1 ribosome-inactivating protein isolated from the supernatant of transformed suspension cultures of Trichosanthes kirilowii, Plant Cell Rep., 17, 531, 10.1007/s002990050437 Di Toppi, 1996, Production of ribosome-inactivating protein from hairy root cultures of Luffa cylindrica (L.) Roem, Plant Cell Rep., 15, 910, 10.1007/BF00231586 Knott, 1932, Rapidity of response of spinach to change in photoperiod, Plant Physiol., 7, 125, 10.1104/pp.7.1.125 Zeevaart, 1971, Effects of photoperiod on growth rate and endogenous gibberellins in the long-day rosette plant spinach, Plant Physiol., 47, 821, 10.1104/pp.47.6.821 Takao, 1998, Effects of night lighting and critical illuminance on the growth and bolting of spinach cultivars, J. Jpn. Soc. Hort. Sci., 67, 778, 10.2503/jjshs.67.778 Chun, 2000, Bolting and growth of Spinacia oleracea L. can be altered by modifying the photoperiod during transplant production, Horticulturescience, 35, 624