Identification of a family of zinc transporter genes fromArabidopsisthat respond to zinc deficiency

Proceedings of the National Academy of Sciences of the United States of America - Tập 95 Số 12 - Trang 7220-7224 - 1998
Natasha Grotz1, Tama C. Fox1, Erin L. Connolly1,2, Walter D. Park1, Mary Lou Guerinot1, David Eide1
1Department of Biological Sciences, Dartmouth College, Hanover, NH 03755; Department of Biochemistry and Molecular Biology, University of Minnesota-Duluth, Duluth, MN 55812; and Nutritional Sciences Program, University of Missouri-Columbia, Columbia, MO 65211
2Plant Science

Tóm tắt

Millions of people worldwide suffer from nutritional imbalances of essential metals like zinc. These same metals, along with pollutants like cadmium and lead, contaminate soils at many sites around the world. In addition to posing a threat to human health, these metals can poison plants, livestock, and wildlife. Deciphering how metals are absorbed, transported, and incorporated as protein cofactors may help solve both of these problems. For example, edible plants could be engineered to serve as better dietary sources of metal nutrients, and other plant species could be tailored to remove metal ions from contaminated soils. We report here the cloning of the first zinc transporter genes from plants, theZIP1,ZIP2, andZIP3genes ofArabidopsis thaliana. Expression in yeast of these closely related genes confers zinc uptake activities. In the plant,ZIP1andZIP3are expressed in roots in response to zinc deficiency, suggesting that they transport zinc from the soil into the plant. Although expression ofZIP2has not been detected, a fourth relatedArabidopsisgene identified by genome sequencing,ZIP4, is induced in both shoots and roots of zinc-limited plants. Thus, ZIP4 may transport zinc intracellularly or between plant tissues. These ZIP proteins define a family of metal ion transporters that are found in plants, protozoa, fungi, invertebrates, and vertebrates, making it now possible to address questions of metal ion accumulation and homeostasis in diverse organisms.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 95 Số 12

7220-7224

Thông tin tác giả