Patterns and Dynamics of Subventricular Zone Neuroblast Migration in the Ischemic Striatum of the Adult Mouse

Journal of Cerebral Blood Flow and Metabolism - Tập 29 Số 7 - Trang 1240-1250 - 2009
Rui L. Zhang1, Michael Chopp1,2, Sara R Gregg1, Yier Toh1, Cindi Roberts1, Yvonne LeTourneau1, Benjamin Buller1,2, Longfei Jia1, Siamak P. Nejad‐Davarani1, Zheng G. Zhang1
11Department of Neurology, Henry Ford Health Sciences Center, Detroit, Michigan, USA;
22Physics Department, Oakland University, Rochester, Michigan, USA

Tóm tắt

The migratory behavior of neuroblasts after a stroke is poorly understood. Using time-lapse microscopy, we imaged migration of neuroblasts and cerebral vessels in living brain slices of adult doublecortin (DCX, a marker of neuroblasts) enhanced green fluorescent protein (eGFP) transgenic mice that were subjected to 7 days of stroke. Our results show that neuroblasts originating in the subventricular zone (SVZ) of adult mouse brain laterally migrated in chains or individually to reach the ischemic striatum. The chains were initially formed at the border between the SVZ and the striatum by neuroblasts in the SVZ and then extended to the striatum. The average speed of DCX-eGFP-expressing cells within chains was 28.67 ± 1.04 μm/h, which was significantly faster ( P < 0.01) than the speed of the cells in the SVZ (17.98 ± 0.57 μm/h). Within the ischemic striatum, individual neuroblasts actively extended or retracted their processes, suggestive of probing the immediate microenvironment. The neuroblasts close to cerebral blood vessels exhibited multiple processes. Our data suggest that neuroblasts actively interact with the microenvironment to reach the ischemic striatum by multiple migratory routes.

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Thông tin xuất bản

Journal of Cerebral Blood Flow and Metabolism

Tập 29 Số 7

1240-1250

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