Structure and Molecular Mechanism of a Functional Form of Pneumolysin: A Cholesterol-Dependent Cytolysin from Streptococcus pneumoniae

Bradfford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem., 72, 248, 10.1016/0003-2697(76)90527-3 Chander, 1999, Structural studies on a 2,3-diphosphoglyceric acid independent phosphoglycerate mutase from Bacillus stearothermophilus, J. Struct. Biol., 126, 156, 10.1006/jsbi.1999.4112 Chang, 1978, Circular dichroic analysis of protein conformation: Inclusion of the β-turns, Anal. Biochem., 91, 13, 10.1016/0003-2697(78)90812-6 Gilbert, 1999, Self-interaction of pneumolysin, the pore-forming protein toxin of Streptococcus pneumoniae, Cell, 97, 647, 10.1016/S0092-8674(00)80775-8 Gilbert, 1999, Studies on the structure and mechanism of a bacterial protein toxin by analytical ultracentrifugation and small-angle neutron scattering, J. Mol. Biol., 293, 1145, 10.1006/jmbi.1999.3210 Gilbert, 1998, Self-interaction of pneumolysin, the pore-forming protein toxin of Streptococcus pneumoniae, J. Mol. Biol., 284, 1223, 10.1006/jmbi.1998.2258 Hill, 1994, Amino acids in pneumolysin important for hemolytic activity identified by random mutagenesis, Infect. Immun., 62, 757, 10.1128/IAI.62.2.757-758.1994 Jedrzejas, 2000, Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus, EMBO J., 19, 1419, 10.1093/emboj/19.7.1419 Jedrzejas, 2000, Production and characterization of pneumococcal surface protein A, Arch. Biochem. Biophys., 373, 116, 10.1006/abbi.1999.1544 Jedrzejas, 1998, Expression and purification of Streptococcus pneumoniae hyaluronate lyase from Escherichia coli, Protein Express. Purif., 13, 83, 10.1006/prep.1997.0864 Jedrzejas, 1998, Crystallization and preliminary X-ray analysis of Streptococcus pneumoniae hyaluronate lyase, J. Struct. Biol., 121, 71, 10.1006/jsbi.1998.3963 Johnson, 1981, Analysis of data from the analytical ultracentrifuge by nonlinear least-squares techniques, Biophys. J., 36, 575, 10.1016/S0006-3495(81)84753-4 Korchev, 1997, Scanning ion conductance microscopy of living cells, Biophys. J., 73, 653, 10.1016/S0006-3495(97)78100-1 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Nakamura, 1995, Interaction of theta-toxin (perfringolysin O), a cholesterol-binding cytolysin, with liposomal membranes: Change in the aromatic side chains upon binding and insertion, Biochemistry, 34, 6513, 10.1021/bi00019a032 Pace, 1995, How to measure and predict the molar absorption coefficient of a protein, Prot. Science, 4, 2411, 10.1002/pro.5560041120 Parker, 1994, Structure of the Aeromonas toxin proaerolysin in its water-soluble and membrane-channel states, Nature, 367, 292, 10.1038/367292a0 Parker, 1990, Insights into membrane insertion based on studies of colicins, Trends Biochem. Sci., 15, 126, 10.1016/0968-0004(90)90205-P Paton, 1993, Molecular analysis of the pathogenicity of Streptococcus pneumoniae: The role of pneumococcal proteins, Annu. Rev. Microbiol., 47, 89, 10.1146/annurev.mi.47.100193.000513 Paton, 1986, Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin, Infect. Immun., 54, 50, 10.1128/IAI.54.1.50-55.1986 Paton, 1983, Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae, Infect. Immun., 40, 548, 10.1128/IAI.40.2.548-552.1983 Petosa, 1997, Crystal structure of the anthrax toxin protective antigen, Nature, 385, 833, 10.1038/385833a0 Proksch, 1978, A water-soluble cholesterol derivative for use in augmenting serum control materials, Clin. Chem., 24, 1924, 10.1093/clinchem/24.11.1924 Rayner, 1995, Interaction of pneumolysin-sufficient and -deficient isogenic variants of Streptococcus pneumoniae with human respiratory mucosa, Infect. Immun., 63, 422, 10.1128/IAI.63.2.442-447.1995 Reynolds, 1985, Determination of protein molecular weight in complexes with detergent without knowledge of binding, Methods Enzymol., 117, 41, 10.1016/S0076-6879(85)17005-9 Rossjohn, 1997, Structure of a cholesterol-binding thiol-activated cytolysin and a model of its membrane form, Cell, 89, 685, 10.1016/S0092-8674(00)80251-2 Rossjohn, 1998, The molecular mechanism of pneumolysin, a virulence factor from Streptococcus pneumoniae, J. Mol. Biol., 284, 449, 10.1006/jmbi.1998.2167 Rubins, 1998, Pneumolysin: A multifunctional pneumococcal virulence factor, J. Lab. Clin. Med., 131, 21, 10.1016/S0022-2143(98)90073-7 Shatursky, 1999, The mechanism of membrane insertion for a cholesterol-dependent cytolysin: A novel paradigm for pore-forming toxins, Cell, 99, 293, 10.1016/S0092-8674(00)81660-8 Shepard, 1998, Identification of a membrane-spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O: An alpha-helical to beta-sheet transition identified by fluorescence spectroscopy, Biochemistry, 37, 14563, 10.1021/bi981452f Song, 1996, Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore, Science, 274, 1859, 10.1126/science.274.5294.1859 Stafford, 1992, Boundary analysis in sedimentation transport experiment: A procedure for obtaining sedimentation coefficient distribution using the time derivative of the concentration profile, Anal. Biochem., 203, 295, 10.1016/0003-2697(92)90316-Y Steinfort, 1989, Effects of Streptococcus pneumoniae on human respiratory epithelium in vitro, Infect. Immun., 57, 2006, 10.1128/IAI.57.7.2006-2013.1989 Tanford, 1961 van Holde, 1985 Verner, 1988, Protein translocation across membranes, Science, 241, 1307, 10.1126/science.2842866