Identification of conserved epitopes on a hog cholera virus protein

Archives of Virology - Tập 111 - Trang 213-225 - 1990
I. Greiser-Wilke1, V. Moennig1, C. O. Z. Coulibaly1, J. Dahle1, L. Leder1, B. Liess1
1Institute for Virology, Hannover Veterinary School, Hannover, Federal Republic of Germany

Tóm tắt

Eight monoclonal antibodies directed against the hog cholera virus (HCV) strain Alfort/187 and displaying broad cross-reactivity with other HCV strains were characterized. An enzyme immunoassay on fixed monolayers of porcine or bovine cells infected with 14 different strains and isolates of HCV and 12 bovine viral diarrhea viruses (BVDV), respectively, showed that all antibodies reacted with HCV only. Seven antibodies recognized all HCV tested, thus indicating that they were directed against conserved epitopes. All antibodies neutralized the homologous strain and different patterns of the other HCV tested. Radioimmunoprecipitation analysis showed that the monoclonal antibodies were directed against a doublet of 56–60 kDa, presumably representing the major envelope glycoprotein of HCV. The results of reciprocal antibody blocking assays allowed the mapping of two distinct conserved antigenic domains on this protein.

Tài liệu tham khảo

Aynaud JM (1968) Etude de la multiplication en cycle unique d'un clone du virus de la peste porcine classique au moyen de l'immunofluorescence. Ann Rech Vet 1: 25–36 Bögel K, Voss HJ (1964) Serologische Untersuchungen über die Verbreitung des an der Mucosal-Disease beteiligten Virus in Nordwestdeutschland. Zentralbl Veterinarmed [B] 11: 181–189 Bolin S, Moennig V, Kelso Gourley NE, Ridpath J (1988) Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Arch Virol 99: 117–123 Boorsma DM, Streefkerk JG (1979) Periodate or glutaraldehyde for preparing peroxidase conjugates? J Immunol Methods 30: 245–255 Collett MS, Anderson DK, Retzel E (1988) Comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. J Gen Virol 69: 2637–2643 Collett MS, Larson R, Belzer SK, Retzel E (1988) Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165: 200–208 Collett MS, Larson R, Gold C, Strick D, Anderson DK, Purchio AF (1988) Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165: 191–199 Collett MS, Moennig V, Horzinek MC (1989) Recent advances in pestivirus research. J Gen Virol 70: 253–266 Dahle J, Liess B, Frey H-R (1987) Neutralizing antibody development following sequential inoculation of pigs with strains of bovine viral diarrhea virus and hog cholera virus. J Vet Med [B] 34: 729–739 Darbyshire JH (1960) A serological relationship between swine fever and mucosal disease of cattle. Vet Rec 72: 331 Davis NL, Pence DF, Meyer WJ, Schmaljohn AL, Johnston RE (1987) Alternative forms of a strain-specific neutralizing antigenic site on the Sindbis virus E2 glycoprotein. Virology 161: 101–108 Dräger K, Kamphans S, Maaß W (1964) Bericht über den Einsatz der Schweinepest-Vaccine Suiferin® zur Tilgung der Schweinepest in einem bereits infizierten Schweinezuchtbestand Nord-Deutschlands. Tierärztl Umschau 19: 405–414 Enzmann PJ, Weiland F (1978) Structural similarities of hog cholera virus with togaviruses. Arch Virol 57: 339–348 Fernelius AL, Amtower WC, Malmquist WA, Lambert G, Mathews PI (1973) Bovine viral diarrhea virus in swine: neutralizing antibody in naturally and experimentally infected swine. Can J Comp Med 37: 96–102 Frey H-R, Liess B (1971) Vermehrungskinetik und Verwendbarkeit eines stark zytopathogenen VD-MD-Virusstammes für diagnostische Untersuchungen mit der Mikrotiter-Methode. Zentralbl Veterinarmed [B] 18: 61–71 Gillespie JH, Baker JA, McEntee K (1960) A cytopathogenic strain of virus diarrhea virus. Cornell Vet 50: 73–79 Gutekunst DE, Malmquist WA (1963) Separation of a soluble antigen and infectious particles of bovine viral diarrhea viruses and their relationship to hog cholera. Can J Comp Med 27: 121–123 Hafez SM (1967) Charakterisierung und Darstellung des Virus der ‘Virusdiarrhoe-Mucosal Disease’ des Rindes (VD-Virus). Thesis, University of Hannover, Hannover, Federal Republic of Germany Heinz FX, Berger R, Tuma W, Kunz C (1983) Location of immunodominant antigenic determinants on fragments of the tick-borne encephalitis virus glycoprotein: evidence for two different mechanisms by which antibodies mediate neutralization and hemagglutination inhibition. Virology 130: 485–501 Hess RG, Coulibaly COZ, Greiser-Wilke I, Moennig V, Liess B (1988) Identification of hog cholera viral isolates by use of monoclonal antibodies to pestiviruses. Vet Microbiol 16: 315–321 Holm Jensen M (1981) Detection of antibodies against hog cholera virus and bovine viral diarrhea virus in porcine serum. A comparative examination using CF, PLA, and NPLA assays. Acta Vet Scand 22: 85–98 Horzinek MC (1981) Non-arthropod-borne togaviruses. Academic Press, London Hyera JMK, Liess B, Frey HR (1987) A direct neutralizing peroxidase-linked antibody assay for detection and titration of antibodies to bovine viral diarrhoea virus. J Vet Med [B] 34: 227–239 Kessler SW (1981) Use of protein A-bearing staphylococci for the immunoprecipitation and isolation of antigens from cells. Methods Enzymol 73: 442–459 Köhler G, Howe SC, Milstein C (1976) Fusion between immunoglobulin secreting and nonsecreting myeloma cell lines. Eur J Immunol 6: 292–295 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685 Launais M, Aynaud J-M, Corthier G (1972) Swine Fever virus: properties of a clone (Thiverval strain) isolated in cell culture at low temperature. Use in vaccination. Rev Med Vet 123: 1537–1554 Liess B (1967) Die ätiologische Abgrenzung selbständiger Virusinfektionen, insbesondere der Virusdiarrhoe-Mucosal-Disease im sogenannten “Mucosal-Disease-Komplex” bei Rindern. Deutsch Tierärztl Wochenschr 74: 46–49 Liess B, Frey H-R, Orban S, Hafez SM (1983) Bovine Virusdiarrhoe (BVD)—“Mucosal Disease”: Persistente BVD-Feldvirusinfektionen bei serologisch selektierten Rindern. Deutsch Tierärztl Wochenschr 90: 261–266 Lowry OH, Roseborough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin reagent. J Biol Chem 193: 265–275 Mateo Rosell AM (1988) A topological and functional map of epitopes on the major surface glycoprotein of the bovine viral diarrhoea virus (BVDV). Thesis, University of Hannover, Hannover, Federal Republic of Germany Mengeling WL, Packer RA (1969) Pathogenesis of chronic hog cholera: host response. Am J Vet Res 30: 409–417 Meyers G, Rümenapf T, Thiel H-J (1989) Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology 171: 555–567 Peters W, Greiser-Wilke I, Moennig V, Liess B (1986) Preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. Vet Microbiol 12: 195–200 Pittler H, Brack M, Schulz L, Rohde G, Witte K, Liess B (1968) Untersuchungen über die europäische Schweinepest. I. Mitteilung: Ermittlungen zur gegenwärtigen Seuchensituation in Norddeutschland. Deutsch Tierärztl Wochenschr 75: 537–542 Roehrig JT, Mathews JH (1985) The neutralization site on the E2 glycoprotein of Venezuelan equine encephalomyelitis (TC-83) virus is composed of multiple conformationally stable epitopes. Virology 142: 347–356 Rümenapf T, Meyers G, Stark R, Thiel H-J (1989) Hog cholera virus—characterization of specific antiserum and identification of cDNA clones. Virology 171: 18–27 Sato U, Nishimura Y, Hanaki T, Nobuto K (1964) Attenuation of hog cholera virus by means of continuous cell—virus propagation (CCVP) method. Arch Ges Virusforsch 14: 394–403 Snowdon WA, French EL (1968) The bovine mucosal disease — swine fever virus complex in pigs. Aust Vet 44: 179–184 Stark R, Rümenapf T, Meyers G, Thiel H-J (1989) Genomic localization of hog cholera virus glycoproteins. Virology 174: 286–289 Steinhauer DA, Holland JJ (1987) Rapid evolution of RNA viruses. Annu Rev Microbiol 41: 409–433 Steward MW, Lew AM (1985) The importance of antibody affinity in the performance of immunoassays for antibody. Immunol Methods 78: 173–190 Stone M, Nowinski RC (1980) Topological mapping of murine leukemia virus proteins by competition binding assays with monoclonal antibodies. Virology 100: 370–381 Taggart RT, Samloff IM (1983) Stable antibody-producing murine hybridomas. Science 219: 1228–1230 Wensvoort G, Terpstra C, Boonstra J, Bloemraad M, van Zaane D (1986) Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. Vet Microbiol 12: 101–108 Westaway EG, Brinton MA, Gaidamowich SY, Horzinek MC, Igarashi A, Kääriäinen L, Lvov DK, Porterfield JS, Russel PK, Trent DW (1985) Togaviridae. Intervirology 24: 125–139 Zhou Y, Moennig V, Coulibaly COZ, Dahle J, Liess B (1989) Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies. J Vet Med [B] 36: 76–80