Levels of genetic diversity vary dramatically between Blastocystis subtypes

Abe, 2004, Molecular and phylogenetic analysis of Blastocystis isolates from various hosts, Vet. Parasitol., 25, 235, 10.1016/j.vetpar.2004.01.003 Arisue, 2002, Phylogenetic position of Blastocystis hominis and of stramenopiles inferred from multiple molecular sequence data, J. Eukaryot. Microbiol., 49, 42, 10.1111/j.1550-7408.2002.tb00339.x Arisue, 2003, Sequence heterogeneity of the small subunit ribosomal RNA genes among Blastocystis isolates, Parasitology, 126, 1, 10.1017/S0031182002002640 Bain, 2007, Multilocus sequence typing of the pathogenic fungus Aspergillus fumigatus, J. Clin. Microbiol., 45, 1469, 10.1128/JCM.00064-07 Bougnoux, 2002, Usefulness of multilocus sequence typing for characterization of clinical isolates of Candida albicans, J. Clin. Microbiol., 40, 1290, 10.1128/JCM.40.4.1290-1297.2002 Clark, 2002, Methods for cultivation of luminal parasitic protists of clinical importance, Clin. Microbiol. Rev., 15, 329, 10.1128/CMR.15.3.329-341.2002 Corpet, 1988, Multiple sequence alignment with hierarchical clustering, Nucleic Acids Res., 16, 10881, 10.1093/nar/16.22.10881 Dogruman-Al, 2008, A possible link between subtype 2 and asymptomatic infections of Blastocystis hominis, Parasitol. Res., 103, 685, 10.1007/s00436-008-1031-3 Dogruman-Al, 2009, Blastocystis subtypes in irritable bowel syndrome and inflammatory bowel disease in Ankara, Turkey, Mem. Inst. Oswaldo Cruz, 104, 724, 10.1590/S0074-02762009000500011 Dogruman-Al, 2009, PCR-based subtyping of Blastocystis isolates from symptomatic and asymptomatic individuals in a major hospital in Ankara, Turkey, Parasitol. Res., 106, 263, 10.1007/s00436-009-1658-8 Domínguez-Márquez, 2009, High prevalence of subtype 4 among isolates of Blastocystis hominis from symptomatic patients of a health district of Valencia (Spain), Parasitol. Res., 105, 949, 10.1007/s00436-009-1485-y Eroglu, 2010, Evaluation of the transmission mode of B. Hominis by using PCR method, Parasitol. Res., 107, 841, 10.1007/s00436-010-1937-4 Eroglu, 2009, Identification of Blastocystis hominis isolates from asymptomatic and symptomatic patients by PCR, Parasitol. Res., 105, 1589, 10.1007/s00436-009-1595-6 Forsell, J., Granlund, M., Stensvold, C.R., Clark, C.G., Evengård, B., in press. Subtype analysis of Blastocystis isolates in Swedish patients. Eur. J. Clin. Microbiol. Infect. Dis. Guindon, 2003, A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood, Syst. Biol., 52, 696, 10.1080/10635150390235520 Ho, 2001, Use of the elongation factor-1 alpha gene in a polymerase chain reaction-based restriction-fragment-length-polymorphism analysis of genetic heterogeneity among Blastocystis species, Mol. Biochem. Parasitol., 112, 278, 10.1016/S0166-6851(00)00358-3 Huelsenbeck, 2001, MRBAYES: Bayesian inference of phylogenetic trees, Bioinformatics, 17, 754, 10.1093/bioinformatics/17.8.754 Hunter, 1990, Reproducibility and indices of discriminatory power of microbial typing methods, J. Clin. Microbiol., 28, 1903, 10.1128/JCM.28.9.1903-1905.1990 Hunter, 1988, Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity, J. Clin. Microbiol., 26, 2465, 10.1128/JCM.26.11.2465-2466.1988 Hussein, 2008, Pathophysiological variability of different genotypes of human Blastocystis hominis Egyptian isolates in experimentally infected rats, Parasitol. Res., 102, 853, 10.1007/s00436-007-0833-z Iguchi, 2007, Infectivity of different genotypes of human Blastocystis hominis isolates in chickens and rats, Parasitol. Int., 56, 107, 10.1016/j.parint.2006.12.004 Jolley, 2010, BIGSdb: Scalable analysis of bacterial genome variation at the population level, BMC Bioinformatics, 11, 595, 10.1186/1471-2105-11-595 Jones, 2008, Detection of Blastocystis from stool samples using real-time PCR, Parasitol. Res., 103, 551, 10.1007/s00436-008-1006-4 Kaneda, 2001, Ribodemes of Blastocystis hominis isolated in Japan, Am. J. Trop. Med. Hyg., 65, 393, 10.4269/ajtmh.2001.65.393 Lantsman, 2008, Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7, Microbiology, 154, 2757, 10.1099/mic.0.2008/017897-0 Leelayoova, 2008, Drinking water: a possible source of Blastocystis spp. subtype 1 infection in schoolchildren of a rural community in central Thailand, Am. J. Trop. Med. Hyg., 79, 401, 10.4269/ajtmh.2008.79.401 Leipe, 1996, 16S-like rDNA sequences from Developayella elegans, Labyrinthuloides haliotidis, and Proteromonas lacertae confirm that the stramenopiles are a primarily heterotrophic group, Eur. J. Protistol., 32, 449, 10.1016/S0932-4739(96)80004-6 Li, 2007, Cross-sectional surveys and subtype classification of human Blastocystis isolates from four epidemiological settings in China, Parasitol. Res., 102, 83, 10.1007/s00436-007-0727-0 Li, 2007, Molecular epidemiology of human Blastocystis in a village in Yunnan province, China, Parasitol. Int., 56, 281, 10.1016/j.parint.2007.06.001 Librado, 2009, DnaSP v5: a software for comprehensive analysis of DNA polymorphism data, Bioinformatics, 25, 1451, 10.1093/bioinformatics/btp187 Malheiros, A.F., Stensvold, C.R., Clark, C.G., Braga, G.B., Shaw, J.J., in press. Molecular characterisation of Blastocystis obtained from members of the indigenous Tapirapé Ethnic Group from the Brazilian Amazon Region, Brazil. Am. J. Trop. Med. Hyg. Meloni, 2011, Molecular subtyping of Blastocystis sp. isolates from symptomatic patients in Italy, Parasitol. Res., 109, 613, 10.1007/s00436-011-2294-7 Noël, 2003, Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences, Mol. Biochem. Parasitol., 126, 119, 10.1016/S0166-6851(02)00246-3 Noël, 2005, Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis, J. Clin. Microbiol., 43, 348, 10.1128/JCM.43.1.348-355.2005 Ozyurt, 2008, Molecular epidemiology of Blastocystis infections in Turkey, Parasitol. Int., 57, 300, 10.1016/j.parint.2008.01.004 Parkar, 2007, Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential, Parasitology, 134, 359, 10.1017/S0031182006001582 Parkar, 2010, Molecular characterization of Blastocystis isolates from zoo animals and their animal-keepers, Vet. Parasitol., 169, 8, 10.1016/j.vetpar.2009.12.032 Pérez-Brocal, 2008, Analysis of two genomes from the mitochondrion-like organelle of the intestinal parasite Blastocystis: complete sequences, gene content, and genome organization, Mol. Biol. Evol., 25, 2475, 10.1093/molbev/msn193 Petrášová, 2011, Diversity and host specificity of Blastocystis in syntopic primates on Rubondo Island, Tanzania, Int. J. Parasitol., 41, 1113, 10.1016/j.ijpara.2011.06.010 Rene, 2009, Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients, Am. J. Trop. Med. Hyg., 80, 588, 10.4269/ajtmh.2009.80.588 Rivera, 2008, Phylogenetic analysis of Blastocystis isolates from animal and human hosts in the Philippines, Vet. Parasitol., 156, 178, 10.1016/j.vetpar.2008.06.001 Santín, 2011, Development of a new PCR protocol to detect and subtype Blastocystis spp. from humans and animals, Parasitol. Res., 109, 205, 10.1007/s00436-010-2244-9 Scanlan, 2008, Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and -independent analysis of faeces, ISME J., 2, 1183, 10.1038/ismej.2008.76 Scicluna, 2006, DNA barcoding of Blastocystis, Protist, 157, 77, 10.1016/j.protis.2005.12.001 Silberman, 1996, Human parasite finds taxonomic home, Nature, 380, 398, 10.1038/380398a0 Souppart, 2009, Molecular epidemiology of human Blastocystis isolates in France, Parasitol. Res., 105, 413, 10.1007/s00436-009-1398-9 Souppart, 2010, Subtype analysis of Blastocystis isolates from symptomatic patients in Egypt, Parasitol. Res., 106, 505, 10.1007/s00436-009-1693-5 Staden, 2000, The Staden package, 1998, Methods Mol. Biol., 132, 115 Stechmann, 2008, Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes, Curr. Biol., 18, 580, 10.1016/j.cub.2008.03.037 Stensvold, 2006, Detection of Blastocystis hominis in unpreserved stool specimens using polymerase chain reaction, J. Parasitol., 92, 1081, 10.1645/GE-840R.1 Stensvold, 2007, Detecting Blastocystis using parasitologic and DNA-based methods: a comparative study, Diagn. Microbiol. Infect. Dis., 59, 303, 10.1016/j.diagmicrobio.2007.06.003 Stensvold, 2007, Terminology for Blastocystis subtypes – a consensus, Trends Parasitol., 23, 93, 10.1016/j.pt.2007.01.004 Stensvold, 2009, Subtype distribution of Blastocystis isolates from synanthropic and zoo animals and identification of a new Blastocystis sp. Subtype, Int. J. Parasitol., 39, 473, 10.1016/j.ijpara.2008.07.006 Stensvold, 2009, Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite, Epidemiol. Infect., 137, 1655, 10.1017/S0950268809002672 Stensvold, 2009, Pursuing the clinical significance of Blastocystis – diagnostic limitations, Trends Parasitol., 25, 23, 10.1016/j.pt.2008.09.010 Stensvold, 2011, Blastocystis sp. subtype 4 is common in Danish Blastocystis-positive patients presenting with acute diarrhea, Am. J. Trop. Med. Hyg., 84, 883, 10.4269/ajtmh.2011.11-0005 Stenzel, 1996, Blastocystis hominis revisited, Clin. Microbiol. Rev., 9, 563, 10.1128/CMR.9.4.563 Sullivan, 2005, Multilocus sequence typing: data analysis in clinical microbiology and public health, Mol. Biotechnol., 29, 245, 10.1385/MB:29:3:245 Tamura, 2011, MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods, Mol. Biol. Evol., 28, 2731, 10.1093/molbev/msr121 Tan, 2008, Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential, Parasitol. Res., 104, 85, 10.1007/s00436-008-1163-5 Tan, 2009, Genetic variability of Blastocystis sp. isolates obtained from cancer and HIV/AIDS patients, Parasitol. Res., 105, 1283, 10.1007/s00436-009-1551-5 Wawrzyniak, 2008, Complete circular DNA in the mitochondria-like organelles of Blastocystis hominis, Int. J. Parasitol., 38, 1377, 10.1016/j.ijpara.2008.06.001 Whipps, 2010, Molecular characterization of Blastocystis species in Oregon identifies multiple subtypes, Parasitol. Res., 106, 827, 10.1007/s00436-010-1739-8 Yakoob, 2010, Irritable bowel syndrome: is it associated with genotypes of Blastocystis hominis, Parasitol. Res., 106, 1033, 10.1007/s00436-010-1761-x Yan, 2006, Genetic variability of Blastocystis hominis isolates in China, Parasitol. Res., 99, 597, 10.1007/s00436-006-0186-z Yan, 2007, Blastocystis sp. subtype 5: a possible zoonotic genotype, Parasitol. Res., 101, 1527, 10.1007/s00436-007-0672-y Yeo, 2011, Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi, PLoS Negl. Trop. Dis., 5, e1049, 10.1371/journal.pntd.0001049 Yoshikawa, 1998, Genomic polymorphism among Blastocystis hominis strains and development of subtype-specific diagnostic primers, Mol. Cell. Probes, 12, 153, 10.1006/mcpr.1998.0161 Yoshikawa, 2000, Genomic analysis of Blastocystis hominis strains isolated from two long-term health care facilities, J. Clin. Microbiol., 38, 1324, 10.1128/JCM.38.4.1324-1330.2000 Yoshikawa, 2003, Genomic polymorphism among Blastocystis isolates and development of PCR-based identification of zoonotic isolates, J. Eukaryot. Microbiol., 50, 710, 10.1111/j.1550-7408.2003.tb00698.x Yoshikawa, 2004, Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries, Parasitol. Res., 92, 22, 10.1007/s00436-003-0995-2 Yoshikawa, 2009, Molecular characterization of Blastocystis isolates from children and rhesus monkeys in Kathmandu, Nepal, Vet. Parasitol., 160, 295, 10.1016/j.vetpar.2008.11.029