Green synthesized silver nanoparticles using aqueous leaf extracts of Leucas aspera exhibits antimicrobial and catalytic dye degradation properties

Springer Science and Business Media LLC - Tập 1 - Trang 1-8 - 2019
Muthusaravanan Sivaramakrishnan1,2,3, Vivek Jagadeesan Sharavanan1,2,3, Deenadayalan Karaiyagowder Govindarajan1, Yogesan Meganathan1, Bala Subramaniam Devaraj1, Sivarajasekar Natesan2, Ram Kothandan3, Kumaravel Kandaswamy1
1Laboratory of Molecular Biology and Genetic Engineering, Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, India
2Laboratory of Bioremediation Research, Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, India
3Laboratory of Bioinformatics and Computational Biology, Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, India

Tóm tắt

Green synthesis of silver nanoparticles (AgNPs) has been considered as a cost-effective and eco-friendly approach to generate a large volume of functionalized nanoparticles. In this work, AgNPs has been synthesized by reducing silver nitrate using an aqueous leaf extract of Leucas aspera (a medicinal plant found in Indian sub-continent) under ambient conditions. The UV/Vis peak at 428 nm confirmed the formation of AgNPs. FTIR (4500–750 cm−1) analysis revealed the presence of bioactive functional groups coated over synthesized AgNPs. Furthermore, HR-TEM analysis of synthesized AgNPs confirmed the formation of nanostructures. The shape of the AgNPs was found to be spherical with sizes ranging from 20 to 40 nm. In addition, Face Centered Cubic structure of AgNPs was confirmed by XRD analysis. The antimicrobial effect of synthesized AgNPs was also studied in bacterial strains such as Escherichia coli (E. coli) and Bacillus subtilis (B. subtilis). The Minimal Inhibitory Concentration of AgNPs was found to be 30 µg/ml and 15 µg/ml for B. Subtilis and E. coli, respectively. In this study, we have also explored the synergistic effect of AgNPs conjugated with antibiotics such as Ampicillin and Kanamycin, results confirmed an increased antimicrobial activity against E. coli and B. subtilis. Finally, we have performed catalytic degradation of recalcitrant textile dyes using AgNPs and found that AgNPs were suitable for degradation of Optilan Red and Lanasyn Blue dyes.

Tài liệu tham khảo

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