Molecular analysis of a full-length sequence of a new yam badnavirus from Dioscorea sansibarensis

Archives of Virology - Tập 152 - Trang 819-825 - 2007
S. Seal1, E. Muller2
1Natural Resources Institute, The University of Greenwich at Medway, Chatham Maritime, U.K.
2CIRAD, UMR BGPI, Montpellier, France

Tóm tắt

Badnavirus-like particles were observed by ISEM in viral preparations from yam (Dioscorea sansibarensis) leaves from Benin. Use of the viral preparation as template for PCR amplification with badnavirus-specific primers gave rise to a 579-bp product with most nucleotide identity (70.8%) to Dioscorea alata bacilliform virus (DaBV, Accession numbers X94575–X94582), the only other yam badnavirus sequenced to date. A full-length badnavirus sequence was generated, which consisted of 7261 nucleotides with a typical Badnavirus genome organisation. The full-length sequence shared most identity (61.9%) to DaBV (Accession numbers X94575–X94582) and hence represents a member of a new badnavirus species termed Dioscorea sansibarensis bacilliform virus (DsBV).

Tài liệu tham khảo

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