Relationship of cytomegalovirus load assessed by real-time PCR to pp65 antigenemia in organ transplant recipients

Journal of Clinical Virology - Tập 42 - Trang 335-342 - 2008
Sonali K. Sanghavi1,2, Kareem Abu-Elmagd3, Maria Cristina Keightley1, Kirsten St. George1, Kathleen Lewandowski1, Stephanie S. Boes1, Arlene Bullotta1,2, Ryan Dare1, Maryann Lassak1, Shahid Husain4, Eun J. Kwak4, David L. Paterson4, Charles R. Rinaldo1,2
1Clinical Virology Laboratory, University of Pittsburgh Medical Center, United States
2Department of Pathology, University of Pittsburgh, United States
3Department of Surgery, Thomas E. Starzl Transplantation Institute, University of Pittsburgh Medical Center, United States
4Transplant Infectious Diseases, University of Pittsburgh Medical Center, United States

Tài liệu tham khảo

Bordils, 2005, Comparison of quantitative PCR and antigenemia in cytomegalovirus infection in renal transplant recipients, Transplant Proc, 37, 3756, 10.1016/j.transproceed.2005.10.074 Caliendo, 2000, Comparison of quantitative cytomegalovirus (CMV) PCR in plasma and CMV antigenemia assay: clinical utility of the prototype AMPLICOR CMV MONITOR test in transplant recipients, J Clin Microbiol, 38, 2122, 10.1128/JCM.38.6.2122-2127.2000 Gallina, 1996, Human cytomegalovirus pp65 lower matrix phosphoprotein harbours two transplantable nuclear localization signals, J Gen Virol, 77, 1151, 10.1099/0022-1317-77-6-1151 Gerna, 2007, Evaluation of cytomegalovirus DNAaemia versus pp65-antigenaemia cutoff for guiding preemptive therapy in transplant recipients: a randomized study, Antivir Ther, 12, 63, 10.1177/135965350701200108 Gerna, 2000, Human cytomegalovirus replicates abortively in polymorphonuclear leukocytes after transfer from infected endothelial cells via transient microfusion events, J Virol, 74, 5629, 10.1128/JVI.74.12.5629-5638.2000 Grundy, 1998, Cytomegalovirus-infected endothelial cells recruit neutrophils by the secretion of C-X-C chemokines and transmit virus by direct neutrophil-endothelial cell contact and during neutrophil transendothelial migration, J Infect Dis, 177, 1465, 10.1086/515300 Hernando, 2005, Comparison of cytomegalovirus viral load measure by real-time PCR with pp65 antigenemia for the diagnosis of cytomegalovirus disease in solid organ transplant patients, Transplant Proc, 37, 4094, 10.1016/j.transproceed.2005.10.087 Huggett, 2005, Real-time RT-PCR normalisation; strategies and considerations, Genes Immun, 6, 279, 10.1038/sj.gene.6364190 Ikewaki, 2005, Real-time PCR assays based on distinct genomic regions for cytomegalovirus reactivation following hematopoietic stem cell transplantation, Bone Marrow Transplant, 35, 403, 10.1038/sj.bmt.1704791 Kas-Deelen, 2001, Uptake of pp65 in in vitro generated pp65-positive polymorphonuclear cells mediated by phagocytosis and cell fusion?, Intervirology, 44, 8, 10.1159/000050024 Kalpoe, 2004, Validation of clinical application of cytomegalovirus plasma DNA load measurement and definition of treatment criteria by analysis of correlation to antigen detection, J Clin Microbiol, 42, 1498, 10.1128/JCM.42.4.1498-1504.2004 Kim, 2007, Real-time PCR assay compared with antigenemia assay for detecting cytomegalovirus infection in kidney transplant recipients, Transplant Proc, 39, 1458, 10.1016/j.transproceed.2007.01.088 Kusne, 1999, Prevention and treatment of cytomegalovirus infection in organ transplant recipients, Transplant Infect Dis, 1, 187, 10.1034/j.1399-3062.1999.010307.x Kusne, 1999, Cytomegalovirus PP65 antigenemia monitoring as a guide for preemptive therapy: a cost effective strategy for prevention of cytomegalovirus disease in adult liver transplant recipients, Transplantation, 68, 1125, 10.1097/00007890-199910270-00011 Li, 2003, Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients, J Clin Microbiol, 41, 187, 10.1128/JCM.41.1.187-191.2003 Lilleri, 2007, Use of a DNAemia cut-off for monitoring human cytomegalovirus infection reduces the number of preemptively treated children and young adults receiving hematopoietic stem-cell transplantation compared with qualitative pp65 antigenemia, Blood, 110, 2757, 10.1182/blood-2007-03-080820 Lilleri, 2004, Clinically based determination of safe DNAemia cutoff levels for preemptive therapy or human cytomegalovirus infections in solid organ and hematopoietic stem cell transplant recipients, J Med Virol, 73, 412, 10.1002/jmv.20107 Ljungman, 2002, Definitions of cytomegalovirus infection and disease in transplant recipients, Clin Infect Dis, 34, 1094, 10.1086/339329 Razonable, 2002, The clinical use of various blood compartments for cytomegalovirus (CMV) DNA quantitation in transplant recipients with CMV disease, Transplantation, 73, 968, 10.1097/00007890-200203270-00025 Razonable, 2004, Management of CMV infection and disease in transplant patients, Herpes, 11, 77 Rinaldo, 1977, Interaction of cytomegalovirus with leukocytes from patients with mononucleosis due to cytomegalovirus, J Infect Dis, 136, 667, 10.1093/infdis/136.5.667 Roback, 2001, Multicenter evaluation of PCR methods for detecting CMV DNA in blood donors, Transfusion, 41, 1249, 10.1046/j.1537-2995.2001.41101249.x Ruell, 2007, Active CMV disease does not always correlate with viral load detection, Bone Marrow Transplant, 40, 55, 10.1038/sj.bmt.1705671 Schmolke, 1995, Nuclear targeting of the tegument protein pp65 (UL83) of human cytomegalovirus: an unusual bipartite nuclear localization signal functions with other portions of the protein to mediate its efficient nuclear transport, J Virol, 69, 1071, 10.1128/JVI.69.2.1071-1078.1995 Slifkin, 2001, Late and atypical cytomegalovirus disease in solid-organ transplant recipients, Clin Infect Dis, 33, E62, 10.1086/322663 St. George, 1997, Comparison of commercially available antibody reagents for the cytomegalovirus pp65 antigenemia assay, Clin Diagn Virol, 7, 147, 10.1016/S0928-0197(96)00264-4 Stocher, 2002, Normalized quantification of human cytomegalovirus DNA by competitive real-time PCR on the LightCycler instrument, J Clin Microbiol, 40, 4547, 10.1128/JCM.40.12.4547-4553.2002 van Doornum, 2003, Diagnosing herpesvirus infections by real-time amplification and rapid culture, J Clin Microbiol, 41, 576, 10.1128/JCM.41.2.576-580.2003 Zanghellini, 1999, Asymptomatic primary cytomegalovirus infection: virologic and immunologic features, J Infect Dis, 180, 702, 10.1086/314939 Zhang, 1995, Detection of human cytomegalovirus DNA, RNA, and antibody in normal donor blood, J Infect Dis, 171, 1002, 10.1093/infdis/171.4.1002