Optimized expression of soluble cyclomaltodextrinase of thermophilic origin in Escherichia coli by using a soluble fusion-tag and by tuning of inducer concentration

Georgiou, 1996, Expression of correctly folded proteins in Escherichia coli, Curr. Opin. Biotechnol., 7, 190, 10.1016/S0958-1669(96)80012-7 De Bernardez Clark, 1998, Refolding of recombinant proteins, Curr. Opin. Biotechnol., 9, 157, 10.1016/S0958-1669(98)80109-2 Hellman, 1995, In vitro refolding of cyclomaltodextrin glucanotransferase from cytoplasmic inclusion bodies formed upon expression in Escherichia coli, Protein Expr. Purif., 6, 56, 10.1006/prep.1995.1008 Martins, 2003, Sequence analysis of cyclodextrin glycosyltransferase from the alkaliphilic Bacillus agaradhaerens, Biotechnol. Lett., 25, 1555, 10.1023/A:1025430532333 Thomas, 1996, Protein misfolding and inclusion body formation in recombinant Escherichia coli cells overexpressing heat-shock proteins, J. Biol. Chem., 271, 11141, 10.1074/jbc.271.19.11141 Vasina, 1997, Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the Escherichia coli cspA and tac promoter systems, Protein Expr. Purif., 9, 211, 10.1006/prep.1996.0678 Yin, 2003, Evaluation of different promoters and host strains for the high-level expression of collagen-like polymer in Escherichia coli, J. Biotechnol., 100, 181, 10.1016/S0168-1656(02)00249-3 Chao, 2002, Stringent regulation and high-level expression of heterologous genes in Escherichia coli using T7 system controllable by the araBAD promoter, Biotechnol. Prog., 18, 394, 10.1021/bp0101785 Newman, 1999, Broad-host-range expression vectors that carry the l-arabinose-inducible Escherichia coli araBAD promoter and the araC regulator, Gene, 227, 197, 10.1016/S0378-1119(98)00601-5 Brixey, 1997, The chloroplast psbA promoter is more efficient in Escherichia coli than the T7 promoter for hyperexpression of a foreign protein, Biotechnol. Lett., 19, 395, 10.1023/A:1018371405675 Kapust, 1999, Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused, Protein Sci., 8, 1668, 10.1110/ps.8.8.1668 La Vallie, 2000, Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli, Methods Enzymol., 326, 322, 10.1016/S0076-6879(00)26063-1 Zhang, 1998, Expression of eukaryotic proteins in soluble form in Escherichia coli, Protein Expr. Purif., 12, 159, 10.1006/prep.1997.0834 Forrer, 1998, High-level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage lambda head protein D, Gene, 224, 45, 10.1016/S0378-1119(98)00538-1 Davis, 1999, New fusion protein systems designed to give soluble expression in Escherichia coli, Biotechnol. Bioeng., 65, 382, 10.1002/(SICI)1097-0290(19991120)65:4<382::AID-BIT2>3.0.CO;2-I Miller, 1959, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, 426, 10.1021/ac60147a030 Laemmli, 1970, Cleavage of structural proteins during assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Park, 2001, The multisubstrate specificity and the quaternary structure of cyclodextrin-/pullulan-degrading enzymes, J. Appl. Glycosci., 48, 293, 10.5458/jag.48.293 Wilkinson, 1991, Predicting the solubility of recombinant proteins in Escherichia coli, Bio/Technology, 9, 443, 10.1038/nbt0591-443 Hammarström, 2002, Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli, Protein Sci., 11, 313, 10.1110/ps.22102