Reciprocal induction of tumor necrosis factor‐α and interleukin‐β activity mediates fibronectin synthesis in coronary artery smooth muscle cells

Journal of Cellular Physiology - Tập 163 Số 1 - Trang 19-29 - 1995
Silvana Molossi1,2, Nadine Clausell1,2, Marlene Rabinovitch1,2
1Departments of Pediatrics, Pathology and Medicine, University of Toronto, Toronto, Ontario, Canada
2Division of Cardiovascular Research, Research Institute, The Hospital For Sick Children, Toronto, Ontario, Canada, M5G 1X8

Tóm tắt

AbstractWe previously demonstrated an immune‐inflammatory response associated with increased expression of interleukin (IL)‐β and fibronectin in graft coronary arteriopathy in piglets following heterotopic heart transplant. Further studies showed that increased endogenously produced IL‐β was upregulating fibronectin production by donor coronary artery (CA) smooth muscle cells (SMC). Since co‐induction of IL‐β and tumor necrosis factor (TNF)‐α has been shown in other systems, we investigated the possible interaction between these cytokines in regulating fibronectin production in CA SMC. First, we documented increased TNF‐α expression in vivo in donor compared to host CA. Next, synthesis of fibronectin was measured in host and donor CA SMC following [35S]‐methionine radiolabeling and gelatin‐sepharose extraction. As previously shown with IL‐β, increased donor CA SMC fibronectin synthesis was reduced to host levels in the presence of TNF‐α antibodies, and exogenous TNF‐α upregulated fibronectin synthesis in host CA SMC to levels in donor cells. In normal CA SMC, TNF‐α‐stimulated fibronectin production was downregulated to or below control levels in the presence of IL‐β antibodies. Likewise, IL‐β‐stimulated fibronectin synthesis was downregulated to control levels when TNF‐α neutralizing antibodies were added. Combining TNF‐α and IL‐β enhanced fibronectin production over that observed with either cytokine alone, but was not additive. Thus, our studies suggest that vascular SMC fibronectin synthesis is regulated by reciprocal induction of IL‐β and TNF‐α activity and provide the first demonstration of a ‘cytokine loop’ modulating matrix production. © 1995 Wiley‐Liss, Inc.

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Tài liệu tham khảo

Arbustini E., 1991, Expression of tumor necrosis factor in human acute cardiac rejection, Am. J. Pathol., 139, 709

10.1002/jcp.1041520207

Boudreau N., 1991, Developmentally regulated changes in extracellular matrix in endothelial and smooth muscle cells in the ductus arteriosus may be related to intimal proliferation, Lab. Invest., 64, 187

10.1016/0012-1606(91)90074-D

10.1016/0925-4439(93)90086-G

10.1002/jcp.1041590205

10.1172/JCI116776

Clausell N., 1993, Increased interleukin‐1β and fibronectin expression are early features of the development of the post‐cardiac transplant coronary arteriopathy in piglets, Am. J. Pathol., 142, 1772

10.1161/01.CIR.89.6.2768

10.1182/blood.V77.8.1627.1627

10.1084/jem.163.6.1433

Fanslow W., 1991, Regulation of alloreactivity in vivo by a soluble form of interleukin‐1 receptor, Science, 218, 639

10.1097/00007890-199009000-00020

Gao S., 1990, Accelerated transplant coronary artery disease, Semin. Thorac. Cardiovasc. Surg., 2, 241

Gorospe M., 1993, Tumor necrosis factor increases stability of interleukin‐1 mRNA by activating protein kinase C, J. Biol. Chem., 268, 6214, 10.1016/S0021-9258(18)53241-5

Gown A., 1986, Human atherosclerosis. II. Immunocytochemical analysis of the cellular composition of human atherosclerotic lesions, Am. J. Pathol., 125, 191

Hancock W., 1991, TNF as a mediator of cardiac transplant rejection, including effects on the intra‐graft protein C/protein S/thrombomodulin pathway, Transplant. Proc., 23, 235

Hershkoviz R., 1993, Extracellular matrix induces tumor necrosis factor‐α secretion by an interaction between resting rat CD4+ T cells and macrophages, Immunology, 78, 50

Hu W., 1993, Transcriptional regulation of fibronectin by interleukin‐1β related to accelerated graft atherosclerosis following cardiac transplant, Circulation, 88, 920

10.1097/00007890-199008000-00009

10.1097/00007890-199101000-00008

10.1016/0167-4889(93)90172-L

10.1002/jcp.1041440304

Koike K., 1988, Telemetric detection of cardiac allograft rejection. Correlation of electrophysiological, histological, and biochemical changes during unmodified rejection, Circulation, 78, I106

10.1097/00007890-199201000-00031

Kruppa G., 1992, Inhibition of tumor necrosis factor (TNF)‐mediated NF‐kB activation by selective blockade of the human 55‐kDa TNF receptor, J. Immunol., 148, 3152, 10.4049/jimmunol.148.10.3152

Le J., 1987, Tumor necrosis factor and interleukin‐1: Cytokines with multiple overlapping biological activities, Lab. Invest., 56, 234

10.1002/9780470110393.ch2

Libby P., 1989, Functions of vascular wall cells related to development of transplantation‐associated coronary arteriosclerosis, Transplant. Proc., 21, 3677

10.1016/0012-1606(88)90378-8

10.1172/JCI113540

10.1016/0014-5793(88)80283-7

Molossi S., 1993, Coronary artery endothelial interleukin‐1β mediates enhanced fibronectin production related to post‐cardiac transplant arteriopathy in piglets, Circulation, 88, 248

Molossi S., 1994, Blockade of interleukin‐1β‐induced fibronectin/lymphocyte interaction in vitro inhibits lymphocyte transendothelial migration, FASEB J., 8, A1018

10.1084/jem.163.6.1363

10.1097/00007890-199212000-00015

Piguet P., 1992, Evolution of collagen arthritis in mice is arrested by treatment with anti‐tumour necrosis factor (TNF) antibody or a recombinant soluble TNF receptor, Immunology, 77, 510

10.1083/jcb.50.1.172

10.1084/jem.162.4.1208

Salomon R., 1991, Human coronary transplantation‐associated arteriosclerosis, Am. J. Pathol., 138, 791

10.1161/01.CIR.76.4.827

Vandernbroeke C., 1991, Differential in situ expression of cytokines in renal allograft rejection, Transplantation, 51, 601

Varani J., 1989, Modulation of fibronectin production in normal human melanocytes and malignant melanoma cells by interferon‐γ and tumor necrosis factor‐α, Am. J. Pathol., 134, 827

Warner S., 1987, Interleukin 1 induces Interleukin 1 II. Recombinant human interleukin 1 induces interleukin 1 production by adult human vascular endothelial cells, J. Immunol., 139, 1911, 10.4049/jimmunol.139.6.1911

10.1083/jcb.106.3.915

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Journal of Cellular Physiology

Tập 163 Số 1

19-29

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