Continuous Monitoring of Extracellular Glucose Concentrations in the Striatum of Freely Moving Rats with an Implanted Glucose Biosensor

Journal of Neurochemistry - Tập 70 Số 1 - Trang 391-396 - 1998
John Lowry1,2, Robert O’Neill1,2, Martyn G. Boutelle3,2, Marianne Fillenz2
1The current address of Dr. J. P. Lowry and Dr. R. D. O'Neill is Department of Chemistry, University College Dublin, Belfield, Dublin 4, Ireland.
2University Laboratory of Physiology, Oxford, England
3The current address of Dr. M. G. Boutelle is Department of Chemistry, King's College London, Strand, London WC2R 2LS, U.K.

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Abstract: We have used a glucose oxidase‐based sensor implanted in the striatum of freely moving rats to determine the concentration of extracellular glucose in two distinct ways. With a modification of the zero net flux method, in which different concentrations of glucose are infused through a dialysis probe glued to the biosensor, we calculated the concentration at which there was no change in glucose current by regression analysis; this gave a concentration of 0.351 ± 0.016 mM. Calculating the concentration from the basal current and the in vitro calibration of the biosensor was not significantly different from this. The basal extracellular glucose concentration determined by either method remained constant over a period of several days. Infusion of 50 µM veratridine through the adjacent dialysis probe caused a steep decrease in glucose current as soon as the drug reached the brain in contrast to the delayed fall (7.5 min) seen with microdialysis in previous experiments from this laboratory. These results demonstrate that this biosensor provides a direct, real‐time measure of the extracellular concentration of glucose.

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Journal of Neurochemistry

Tập 70 Số 1

391-396

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