Accumulation of miR-155 and BIC RNA in human B cell lymphomas

Proceedings of the National Academy of Sciences of the United States of America - Tập 102 Số 10 - Trang 3627-3632 - 2005
Peggy S. Eis1, Wayne Tam1, Liping Sun1, Amy Chadburn1, Zongdong Li1, Mario Gomez1, Elsebet Lund1, James E. Dahlberg1
1Department of Biomolecular Chemistry, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706; and Department of Pathology and Laboratory Medicine, The Joan and Sanford I. Weill Medical College of Cornell University, 525 East 68th Street, New York, NY 10021

Tóm tắt

We show that the microRNA miR-155 can be processed from sequences present in BIC RNA, a spliced and polyadenylated but non-protein-coding RNA that accumulates in lymphoma cells. The precursor of miR-155 is likely a transient spliced or unspliced nuclear BIC transcript rather than accumulated BIC RNA, which is primarily cytoplasmic. By using a sensitive and quantitative assay, we find that clinical isolates of several types of B cell lymphomas, including diffuse large B cell lymphoma (DLBCL), have 10- to 30-fold higher copy numbers of miR-155 than do normal circulating B cells. Similarly, the quantities of BIC RNA are elevated in lymphoma cells, but ratios of the amounts of the two RNAs are not constant, suggesting that the level of miR-155 is controlled by transcription and processing. Significantly higher levels of miR-155 are present in DLBCLs with an activated B cell phenotype than with the germinal center phenotype. Because patients with activated B cell-type DLBCL have a poorer clinical prognosis, quantification of this microRNA may be diagnostically useful.

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Proceedings of the National Academy of Sciences of the United States of America

Tập 102 Số 10

3627-3632

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