Highly parallel microbial diagnostics using oligonucleotide microarrays
Tài liệu tham khảo
Bodrossy, 2003, Development and validation of a diagnostic microbial microarray for methanotrophs, Environ Microbiol, 5, 566, 10.1046/j.1462-2920.2003.00450.x
Zhou, 2003, Microarrays for bacterial detection and microbial community analysis, Curr Opin Microbiol, 6, 288, 10.1016/S1369-5274(03)00052-3
Letowski, 2003, DNA microarray applications in environmental microbiology, Anal Lett, 36, 3165, 10.1081/AL-120026566
Stenger, 2002, Potential applications of DNA microarrays in biodefense-related diagnostics, Curr Opin Biotechnol, 13, 208, 10.1016/S0958-1669(02)00321-X
Clewley, 2004, A role for arrays in clinical virology: fact or fiction?, J Clin Virol, 29, 2, 10.1016/j.jcv.2003.08.002
Gershon, 2004, Microarrays go mainstream, Nature Methods, 1, 263, 10.1038/nmeth1204-263
Guschin, 1997, Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology, Appl Environ Microbiol, 63, 2397, 10.1128/AEM.63.6.2397-2402.1997
Fotin, 1998, Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips, Nucleic Acids Res, 26, 1515, 10.1093/nar/26.6.1515
Anthony, 2005, Direct detection of Staphylococcus aureus mRNA using a flow through microarray, J Microbiol Methods, 60, 47, 10.1016/j.mimet.2004.08.009
Wu, 2004, Quantitative assessment of a novel flow-through porous microarray for the rapid analysis of gene expression profiles, Nucleic Acids Res, 32, e123, 10.1093/nar/gnh118
Urakawa, 2002, Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses, Appl Environ Microbiol, 68, 235, 10.1128/AEM.68.1.235-244.2002
Urakawa, 2003, Optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays, Appl Environ Microbiol, 69, 2848, 10.1128/AEM.69.5.2848-2856.2003
Bodrossy, 2004, Oligonucleotide microarrays in microbial diagnostics, Curr Opin Microbiol, 7, 245, 10.1016/j.mib.2004.04.005
Ludwig, 1999, Phylogeny of bacteria beyond the 16S rRNA standard, ASM News, 65, 1515
Loy, 2002, Oligonucleotide microarray for 16S rRNA gene-based detection of all recognized lineages of sulfate-reducing prokaryotes in the environment, Appl Environ Microbiol, 68, 5064, 10.1128/AEM.68.10.5064-5081.2002
Loy, 2003, probeBase: an online resource for rRNA-targeted oligonucleotide probes, Nucleic Acids Res, 31, 514, 10.1093/nar/gkg016
Wilson, 2002, High-density microarray of small-subunit ribosomal DNA probes, Appl Environ Microbiol, 68, 2535, 10.1128/AEM.68.5.2535-2541.2002
Warsen, 2004, Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays, Appl Environ Microbiol, 70, 4216, 10.1128/AEM.70.7.4216-4221.2004
Ludwig, 2004, ARB: a software environment for sequence data, Nucleic Acids Res, 32, 1363, 10.1093/nar/gkh293
Cole, 2003, The ribosomal database project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy, Nucleic Acids Res, 31, 442, 10.1093/nar/gkg039
Mitterer, 2004, Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences, J Clin Microbiol, 42, 1048, 10.1128/JCM.42.3.1048-1057.2004
Nubel, 2004, Oligonucleotide microarrays for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA, FEMS Microbiol Lett, 240, 215, 10.1016/j.femsle.2004.09.042
Dahllof, 2000, rpoB-based microbial community analysis avoids limitations inherent in 16S rRNA gene intraspecies heterogeneity, Appl Environ Microbiol, 66, 3376, 10.1128/AEM.66.8.3376-3380.2000
Drancourt, 2004, rpoB gene sequence-based identification of aerobic Gram-positive cocci of the genera Streptococcus, Enterococcus, Gemella, Abiotrophia, and Granulicatella, J Clin Microbiol, 42, 497, 10.1128/JCM.42.2.497-504.2004
Mollet, 1997, rpoB sequence analysis as a novel basis for bacterial identification, Mol Microbiol, 26, 1005, 10.1046/j.1365-2958.1997.6382009.x
Brisse, 2001, Phylogenetic diversity of Klebsiella pneumoniae and Klebsiella oxytoca clinical isolates revealed by randomly amplified polymorphic DNA, gyrA and parC genes sequencing and automated ribotyping, Int J Syst Evol Microbiol, 51, 915, 10.1099/00207713-51-3-915
Kakinuma, 2003, Detection and identification of Escherichia coli, Shigella, and Salmonella by microarrays using the gyrB gene, Biotechnol Bioeng, 83, 721, 10.1002/bit.10709
Lloyd, 1993, Evolution of the recA gene and the molecular phylogeny of bacteria, J Mol Evol, 37, 399, 10.1007/BF00178869
Baldauf, 1996, The root of the universal tree and the origin of eukaryotes based on elongation factor phylogeny, Proc Natl Acad Sci U S A, 93, 7749, 10.1073/pnas.93.15.7749
Ludwig, 1993, Phylogenetic relationships of Bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes, Antonie Van Leeuwenhoek, 64, 285, 10.1007/BF00873088
Wong, 2002, Identification of enteric pathogens by heat shock protein 60 kDa (HSP60) gene sequences, FEMS Microbiol Lett, 206, 107, 10.1111/j.1574-6968.2002.tb10994.x
Wertz, 2003, A molecular phylogeny of enteric bacteria and implications for a bacterial species concept, J Evol Biol, 16, 1236, 10.1046/j.1420-9101.2003.00612.x
Zwieb, 2003, tmRDB (tmRNA database), Nucleic Acids Res, 31, 446, 10.1093/nar/gkg019
Sergeev, 2004, Simultaneous analysis of multiple staphylococcal enterotoxin genes by an oligonucleotide microarray assay, J Clin Microbiol, 42, 2134, 10.1128/JCM.42.5.2134-2143.2004
Chizhikov, 2001, Microarray analysis of microbial virulence factors, Appl Environ Microbiol, 67, 3258, 10.1128/AEM.67.7.3258-3263.2001
Volokhov, 2002, Identification of Listeria species by microarray-based assay, J Clin Microbiol, 40, 4720, 10.1128/JCM.40.12.4720-4728.2002
Saunders, 2004, A virulence-associated gene microarray: a tool for investigation of the evolution and pathogenic potential of Staphylococcus aureus, Microbiology, 150, 3763, 10.1099/mic.0.27330-0
Grimm, 2004, Use of DNA microarrays for rapid genotyping of TEM beta-lactamases that confer resistance, J Clin Microbiol, 42, 3766, 10.1128/JCM.42.8.3766-3774.2004
Stralis-Pavese, 2004, Optimisation of diagnostic microarray for application in analysing landfill methanotroph communities under different plant covers, Environ Microbiol, 6, 347, 10.1111/j.1462-2920.2004.00582.x
Rhee, 2004, Detection of genes involved in biodegradation and biotransformation in microbial communities by using 50-mer oligonucleotide microarrays, Appl Environ Microbiol, 70, 4303, 10.1128/AEM.70.7.4303-4317.2004
Taroncher-Oldenburg, 2003, Oligonucleotide microarray for the study of functional gene diversity in the nitrogen cycle in the environment, Appl Environ Microbiol, 69, 1159, 10.1128/AEM.69.2.1159-1171.2003
Wu, 2001, Development and evaluation of functional gene arrays for detection of selected genes in the environment, Appl Environ Microbiol, 67, 5780, 10.1128/AEM.67.12.5780-5790.2001
Hashsham, 2004, Potential of DNA microarrays for developing parallel detection tools (PDTs) for microorganisms relevant to biodefense and related research needs, Biosens Bioelectron, 20, 668, 10.1016/j.bios.2004.06.032
Wetmur, 1991, DNA probes: applications of the principles of nucleic acid hybridization, Crit Rev Biochem Mol Biol, 26, 227, 10.3109/10409239109114069
Hughes, 2001, Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer, Nat Biotechnol, 19, 342, 10.1038/86730
Kane, 2000, Assessment of the sensitivity and specificity of oligonucleotide (50mer) microarrays, Nucleic Acids Res, 28, 4552, 10.1093/nar/28.22.4552
Tiquia, 2004, Evaluation of 50-mer oligonucleotide arrays for detecting microbial populations in environmental samples, BioTechniques, 36, 664, 10.2144/04364RR02
Letowski, 2004, Designing better probes: effect of probe size, mismatch position and number on hybridization in DNA oligonucleotide microarrays, J Microbiol Methods, 57, 269, 10.1016/j.mimet.2004.02.002
Vora, 2004, Nucleic acid amplification strategies for DNA microarray-based pathogen detection, Appl Environ Microbiol, 70, 3047, 10.1128/AEM.70.5.3047-3054.2004
Wang, 2002, Microarray-based detection and genotyping of viral pathogens, Proc Natl Acad Sci U S A, 99, 15687, 10.1073/pnas.242579699
Denef, 2003, Validation of a more sensitive method for using spotted oligonucleotide DNA microarrays for functional genomics studies on bacterial communities, Environ Microbiol, 5, 933, 10.1046/j.1462-2920.2003.00490.x
Rouillard, 2003, OligoArray 2.0: design of oligonucleotide probes for DNA microarrays using a thermodynamic approach, Nucleic Acids Res, 31, 3057, 10.1093/nar/gkg426
Gordon, 2004, Osprey: a comprehensive tool employing novel methods for the design of oligonucleotides for DNA sequencing and microarrays, Nucleic Acids Res, 32, e133, 10.1093/nar/gnh127
Behr, 2000, A nested array of rRNA targeted probes for the detection and identification of enterococci by reverse hybridization, Syst Appl Microbiol, 23, 563, 10.1016/S0723-2020(00)80031-4
Wagner, 2003, Fluorescence in situ hybridization for the identification and characterisation of prokaryotes, Curr Opin Microbiol, 6, 302, 10.1016/S1369-5274(03)00054-7
Loy, 2005, 16S rRNA gene-based oligonucleotide microarray for environmental monitoring of the betaproteobacterial order “Rhodocyclales”, Appl Environ Microbiol, 71, 1373, 10.1128/AEM.71.3.1373-1386.2005
Bodrossy, 2003, Diagnostic oligonucleotide microarrays for microbiology, 43
Peplies, 2004, Application and validation of DNA microarrays for the 16S rRNA-based analysis of marine bacterioplankton, Environ Microbiol, 6, 638, 10.1111/j.1462-2920.2004.00588.x
Zuker, 2003, Mfold web server for nucleic acid folding and hybridization prediction, Nucleic Acids Res, 31, 3406, 10.1093/nar/gkg595
SantaLucia, 1998, A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics, Proc Natl Acad Sci U S A, 95, 1460, 10.1073/pnas.95.4.1460
Pfunder, 2005, Dissociation analysis in PCR- and 1xSSC-buffer as a prerequisite for selection of 13mer microarray probe sets with uniform hybiridzation behavior, Mol Biotechnol, 29, 1, 10.1385/MB:29:1:01
Al Khaldi, 2004, Genotyping of Clostridium perfringens toxins using multiple oligonucleotide microarray hybridization, Mol Cell Probes, 18, 359, 10.1016/j.mcp.2004.05.006
Volokhov, 2004, Identification of Bacillus anthracis by multiprobe microarray hybridization, Diagn Microbiol Infect Dis, 49, 163, 10.1016/j.diagmicrobio.2004.03.015
Liu, 2001, Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach, Environ Microbiol, 3, 619, 10.1046/j.1462-2920.2001.00233.x
Huang, 2004, Sum: a new way to incorporate mismatch probe measurements, Genomics, 84, 767, 10.1016/j.ygeno.2004.06.013
Lee, 2004, Guidelines for incorporating non-perfectly matched oligonucleotides into target-specific hybridization probes for a DNA microarray, Nucleic Acids Res, 32, 681, 10.1093/nar/gkh196
Shchepinov, 1997, Steric factors influencing hybridization of nucleic acids to oligonucleotide arrays, Nucleic Acids Res, 25, 1155, 10.1093/nar/25.6.1155
Anthony, 2000, Rapid diagnosis of bacteremia by universal amplification of 23S ribosomal DNA followed by hybridization to an oligonucleotide array, J Clin Microbiol, 38, 781, 10.1128/JCM.38.2.781-788.2000
Matveeva, 2003, Thermodynamic calculations and statistical correlations for oligo-probes design, Nucleic Acids Res, 31, 4211, 10.1093/nar/gkg476
Lane, 2004, Amplicon secondary structure prevents target hybridization to oligonucleotide microarrays, Biosens Bioelectron, 20, 728, 10.1016/j.bios.2004.04.014
Southern, 1995, DNA fingerprinting by hybridization to oligonucleotide arrays, Electrophoresis, 16, 1539, 10.1002/elps.11501601256
Zhang, 2001, Genetic diversity of indigenous Rhizobium leguminosarum bv. viciae isolates nodulating two different host plants during soil restoration with alfalfa, Mol Ecol, 10, 2297, 10.1046/j.0962-1083.2001.01364.x
Chandler, 2003, Sequence versus structure for the direct detection of 16S rRNA on planar oligonucleotide microarrays, Appl Environ Microbiol, 69, 2950, 10.1128/AEM.69.5.2950-2958.2003
Maskos, 1992, Parallel analysis of oligodeoxyribonucleotide (oligonucleotide) interactions. I. Analysis of factors influencing oligonucleotide duplex formation, Nucleic Acids Res, 20, 1675, 10.1093/nar/20.7.1675
Jacobs, 1988, The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones, Nucleic Acids Res, 16, 4637, 10.1093/nar/16.10.4637
Volokhov, 2003, Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis, J Clin Microbiol, 41, 4071, 10.1128/JCM.41.9.4071-4080.2003
Bonch-Osmolovskaya, 2003, Radioisotopic, culture-based, and oligonucleotide microchip analyses of thermophilic microbial communities in a continental high-temperature petroleum reservoir, Appl Environ Microbiol, 69, 6143, 10.1128/AEM.69.10.6143-6151.2003
Koizumi, 2002, Parallel characterization of anaerobic toluene-and ethylbenzene-degrading microbial consortia by PCR-denaturing gradient gel electrophoresis, RNA-DNA membrane hybridization and DNA microarray technology, Appl Environ Microbiol, 68, 3215, 10.1128/AEM.68.7.3215-3225.2002
Wang, 2004, Detection and genotyping of Entamoeba histolytica, Entamoeba dispar, Giardia lamblia, and Cryptosporidium parvum by oligonucleotide microarray, J Clin Microbiol, 42, 3262, 10.1128/JCM.42.7.3262-3271.2004
Lin, 2004, Use of oligonucleotide microarrays for rapid detection and serotyping of acute respiratory disease-associated adenoviruses, J Clin Microbiol, 42, 3232, 10.1128/JCM.42.7.3232-3239.2004
Boriskin, 2004, DNA microarrays for virus detection in cases of central nervous system infection, J Clin Microbiol, 42, 5811, 10.1128/JCM.42.12.5811-5818.2004
Panicker, 2004, Detection of pathogenic vibrio spp. in shellfish by using multiplex PCR and DNA microarrays, Appl Environ Microbiol, 70, 7436, 10.1128/AEM.70.12.7436-7444.2004
Rudi, 2003, A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed, Nucleic Acids Res, 31, e62, 10.1093/nar/gng061
Ericsson, 2003, Microarray-based resequencing by apyrase-mediated allele-specific extension, Electrophoresis, 24, 3330, 10.1002/elps.200305583
Lindroos, 2002, Multiplex SNP genotyping in pooled DNA samples by a four-color microarray system, Nucleic Acids Res, 30, e70, 10.1093/nar/gnf069
Rudi, 2003, Protocols for 16S rDNA array analyses of microbial communities by sequence-specific labeling of DNA probes, Sci World J, 3, 578, 10.1100/tsw.2003.44
Busti, 2002, Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction), BMC Microbiol, 2, 27, 10.1186/1471-2180-2-27
Gharizadeh, 2003, Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays, Nucleic Acids Res, 31, e146, 10.1093/nar/gng147
Lovmar, 2003, Microarrays for genotyping human group a rotavirus by multiplex capture and type-specific primer extension, J Clin Microbiol, 41, 5153, 10.1128/JCM.41.11.5153-5158.2003
Mikhailovich, 2001, Identification of rifampicin-resistant Mycobacterium tuberculosis strains by hybridization, PCR, and ligase detection reaction on oligonucleotide microchips, J Clin Microbiol, 39, 2531, 10.1128/JCM.39.7.2531-2540.2001
Schena, 2003
Rudi K, Treimo J, Moen B, Rud I, Vegarud G. Internal controls for normalizing DNA arrays. BioTechniques 33 (2002) 496, 498, 500.
Cho, 2002, Quantitative detection of microbial genes by using DNA microarrays, Appl Environ Microbiol, 68, 1425, 10.1128/AEM.68.3.1425-1430.2002
Straub, 2002, Genotyping Cryptosporidium parvum with an hsp70 single-nucleotide polymorphism microarray, Appl Environ Microbiol, 68, 1817, 10.1128/AEM.68.4.1817-1826.2002
Gonzalez, 2004, Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray, J Clin Microbiol, 42, 1414, 10.1128/JCM.42.4.1414-1419.2004
Keramas, 2004, Use of culture, PCR analysis, and DNA microarrays for detection of Campylobacter jejuni and Campylobacter coli from chicken feces, J Clin Microbiol, 42, 3985, 10.1128/JCM.42.9.3985-3991.2004
Keramas, 2003, Development of a sensitive DNA microarray suitable for rapid detection of Campylobacter spp, Mol Cell Probes, 17, 187, 10.1016/S0890-8508(03)00052-5
Castiglioni, 2004, Development of a universal microarray based on the ligation detection reaction and 16S rRNA gene polymorphism to target diversity of cyanobacteria, Appl Environ Microbiol, 70, 7161, 10.1128/AEM.70.12.7161-7172.2004
Lehner, 2005, Oligonucleotide microarray for simultaneous identification and differentiation of Enterococcus species in food samples, FEMS Microbiol Lett, 246, 133, 10.1016/j.femsle.2005.04.002
Sergeev, 2004, Multipathogen oligonucleotide microarray for environmental and biodefense applications, Biosens Bioelectron, 20, 684, 10.1016/j.bios.2004.04.030
Peplies, 2005, Application and validation of DNA microarrays for the 16S rRNA-based analysis of marine bacterioplankton, Appl Environ Microbiol, 71, 2723
Fukushima, 2003, Detection and identification of Mycobacterium species isolates by DNA microarray, J Clin Microbiol, 41, 2605, 10.1128/JCM.41.6.2605-2615.2003
Deng, 2004, Multiplex detection of mutations in clinical isolates of rifampin-resistant Mycobacterium tuberculosis by short oligonucleotide ligation assay on DNA chips, J Clin Microbiol, 42, 4850, 10.1128/JCM.42.10.4850-4852.2004
Yu, 2004, Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates, J Clin Microbiol, 42, 4083, 10.1128/JCM.42.9.4083-4091.2004
Roth, 2004, Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections, J Clin Microbiol, 42, 4268, 10.1128/JCM.42.9.4268-4274.2004
Loy, 2004, Microarray and functional gene analyses of sulfate-reducing prokaryotes in low-sulfate, acidic fens reveal cooccurrence of recognized genera and novel lineages, Appl Environ Microbiol, 70, 6998, 10.1128/AEM.70.12.6998-7009.2004
Wilson, 2002, Sequence-specific identification of 18 pathogenic microorganisms using microarray technology, Mol Cell Probes, 16, 119, 10.1006/mcpr.2001.0397
Chizhikov, 2002, Detection and genotyping of human group a rotaviruses by oligonucleotide microarray hybridization, J Clin Microbiol, 40, 2398, 10.1128/JCM.40.7.2398-2407.2002
Klaassen, 2004, DNA microarray format for detection and subtyping of human papillomavirus, J Clin Microbiol, 42, 2152, 10.1128/JCM.42.5.2152-2160.2004
Delrio-Lafreniere, 2004, Low-density addressable array for the detection and typing of the human papillomavirus, Diagn Microbiol Infect Dis, 48, 23, 10.1016/j.diagmicrobio.2003.09.007
Oh, 2004, Development and clinical evaluation of a highly sensitive DNA microarray for detection and genotyping of human papillomaviruses, J Clin Microbiol, 42, 3272, 10.1128/JCM.42.7.3272-3280.2004
Sengupta, 2003, Molecular detection and identification of influenza viruses by oligonucleotide microarray hybridization, J Clin Microbiol, 41, 4542, 10.1128/JCM.41.10.4542-4550.2003
Kessler, 2004, Use of the DNA flow-thru chip, a three-dimensional biochip, for typing and subtyping of influenza viruses, J Clin Microbiol, 42, 2173, 10.1128/JCM.42.5.2173-2185.2004
Laassri, 2003, Detection and discrimination of orthopoxviruses using microarrays of immobilized oligonucleotides, J Virol Methods, 112, 67, 10.1016/S0166-0934(03)00193-9
Lapa, 2002, Species-level identification of orthopoxviruses with an oligonucleotide microchip, J Clin Microbiol, 40, 753, 10.1128/JCM.40.3.753-757.2002
Liu, 2004, Self-contained, fully integrated biochip for sample preparation, polymerase chain reaction amplification, and DNA microarray detection, Anal Chem, 76, 1824, 10.1021/ac0353029
Baner, 2003, Parallel gene analysis with allele-specific padlock probes and tag microarrays, Nucleic Acids Res, 31, e103, 10.1093/nar/gng104
McKendry, 2002, Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array, Proc Natl Acad Sci U S A, 99, 9783, 10.1073/pnas.152330199
Gabig-Ciminska, 2004, Detection of bacteriophage infection and prophage induction in bacterial cultures by means of electric DNA chips, Anal Biochem, 324, 84, 10.1016/j.ab.2003.09.020
Zimmermann, 2003, Consecutive analysis of bacterial PCR samples on a single electronic microarray, J Microbiol Methods, 55, 471, 10.1016/S0167-7012(03)00168-4
Westin, 2001, Antimicrobial resistance and bacterial identification utilizing a microelectronic chip array, J Clin Microbiol, 39, 1097, 10.1128/JCM.39.3.1097-1104.2001
Meunier-Prest, 2003, Direct measurement of the melting temperature of supported DNA by electrochemical method, Nucleic Acids Res, 31, e150, 10.1093/nar/gng150
Stimpson, 1995, Real-time detection of DNA hybridization and melting on oligonucleotide arrays by using optical wave guides, Proc Natl Acad Sci U S A, 92, 6379, 10.1073/pnas.92.14.6379
Nelson, 2002, Label-free detection of 16S ribosomal RNA hybridization on reusable DNA arrays using surface plasmon resonance imaging, Environ Microbiol, 4, 735, 10.1046/j.1462-2920.2002.00350.x
Podsadlowski V, Peter C, Cammann K, Brunen-Nieweler C, Borchers T. Rapid species identification in food samples with an evanescent-field-DNA-biosensor-chip. 2004. Statusseminar Chip Technologies: Microarrays, Hygiene and Health; Frankfurt a. Main, 26.-27.1.2004.