High-performance liquid chromatography of siderophores from fungi
Tóm tắt
A reversed-phase HPLC separation of iron(III) chelates of 16 representative fungal siderophores including ferrichromes, coprogens and triacetylfusarinine C was established in order to investigate siderophore production of fungi. For comparison purposes, the widely used bacterial siderophore ferrioxamine B was included. Culture filtrates of the fungiPenicillium resticulosum, Fusarium dimerum, Aspergillus fumigatus andNeurospora crassa were quantitatively analyzed for the presence of known and unknown siderophores after growth in low-iron culture media and adsorption on XAD-2 columns using this HPLC separation system. Photodiode array detection allowed the distinction between siderophores and non-siderophores. According to their ultraviolet/visible spectra, a further classification of the siderophores into four types due to the number of anhydromevalonic acid residues per molecule (0–3) was possible.
Tài liệu tham khảo
Adjimani JP, Emery T (1987) Iron uptake in Mycelia sterilia EP-76. J Bacteriol 169:3664–3668
Carrano C, Raymond KN (1979) Coordination chemistry of microbial iron transport compounds: rhodotorulic acid and iron uptake inRhodotorula pilimanae. J Bacteriol 136:69–74
Cramer AM, Nathanael B, Horvath C (1984) High-performance liquid chromatography of deferrioxamine and ferrioxamine: Interference by iron present in the chromatographic system. J Chromatogr 295:405–411
Deml G, Voges K, Jung G, Winkelmann G (1984) Tetraglycylferrichrome — the first heptapeptide ferrichrome. FEBS Lett 173:53–57
Diekmann H (1970) Stoffwechselprodukte von Mikroorganismen. Vorkommen und Strukturen von Coprogen B und Dimerumsäure. Arch Microbiol 73:65–76
Diekmann H (1973) Siderochromes (iron III trihydroxamates) In: Laskin AI, Lechevalier HA (eds) Handbook of microbiology, vol III. Microbial products. CRC Press, Cleveland, pp 449–457
Diekmann H, Krezdorn E (1975) Stoffwechselprodukte von Mikroorganismen. Ferricrocin, Triacetylfusigen und andere Sideramine aus Pilzen der GattungAspergillus, GruppeFumigatus. Arch Microbiol 106:191–194
Emery T (1971) Role of ferrichrome as a ferric ionophore inUstilago sphaerogena. Biochemistry 10:1483–1488
Fiedler HP (1981) Preparative-scale high-performance liquid chromatography of ferricrocin, a microbial product. J Chromatogr 209:103–106
Horowitz NH, Charlang G, Horn G, Williams NP (1976) Isolation and identification of the conidial germination factor ofNeurospora crassa. J Bacteriol 127:135–140
Hossain MB, Jalal MAF, Benson BA, Barnes CL, Helm D van der (1987) Structure and conformation of two coprogentype siderophores: neocoprogen I and neocoprogen II. J Am Chem Soc 109:4948–4954
Huschka H, Jalal MAF, Helm D van der, Winkelmann G (1986) Molecular recognition of siderophores in fungi: Role of iron-surroundingN-acyl residues and the peptide backbone during membrane transport inNeurospora crassa. J Bacteriol167:1020–1024
Jalal MAF, Mocharla R, Helm D van der (1984) Separation of ferrichromes and other hydroxamate siderophores of fungal origin by reversed-phase chromatography. J Chromatogr 301:247–252
Jalal MAF, Mocharla R, Barnes CL, Hossain MB, Powell DR, Eng-Wilmot DL, Grayson SL, Benson BA, Helm D van der (1984) Extracellular siderophores fromAspergillus ochraceus. J Bacteriol 158:683–688
Keller-Schierlein W (1963) Stoffwechselprodukte von Mikroorganismen. 45. Mitteilung. Über die Konstitution von Ferrirubin, Ferrirhodin und Ferrichrom A. Helv Chim Acta 46:1920–1929
Keller-Schierlein W, Diekmann H (1970) Stoffwechselprodukte von Mikroorganismen. Zur Konstitution des Coprogens. Helv Chim Acta 53:2035–2044
Matzanke BF, Winkelmann G (1981) Siderophore iron transport followed by Mössbauer spectroscopy. FEBS Lett 130:50–53
Matzanke BF, Bill E, Trautwein AX, Winkelmann G (1987) Metabolic utilization of57Fe-coprogen inNeurospora crassa. An in vivo Mössbauer study. Eur J Biochem 162:643–650
Matzanke BF, Bill E, Trautwein AX, Winkelmann G (1987) Role of siderophores in iron storage in spores ofNeurospora crassa andAspergillus ochraceus. J Bacteriol 169:5873–5876
Moore RE, Emery T (1976)N δ-Acetylfusarinines; isolation, characterization, and properties. Biochemistry 15:2719–2723
Neilands JB (1952) A crystalline organo-iron pigment from a rust fungus (Ustilago sphaerogena). J Am Chem Soc 74:4846–4847
van der Helm D, Jalal MAF, Hossain MB (1987) The crystal structures, conformations, and configurations of siderophores. In: Winkelmann G, Helm D van der, Neilands JB (eds) Iron transport in microbes, plants and animals. VCH Verlagsgesellschaft, Weinheim pp 135–165
Wiebe C, Winkelmann G (1975) Kinetic studies on the specificity of chelate-iron uptake inAspergillus. J Bacteriol 123:837–842
Winkelmann G (1974) Metabolic products of microorganisms. 135. Uptake of iron byNeurospora crassa III. Iron transport studies with ferrichrome-type compounds. Arch Microbiol 98:39–50
Winkelmann G, Helm D van der, Neilands JB (eds) (1987) Iron transport in microbes, plants and animals. VCH Verlagsgesellschaft, Weinheim
Wong GB, Kappel MJ, Raymond KN, Matzanke BF, Winkelmann G (1983) Coordination chemistry of microbial iron transport compounds. 24. Characterization of coprogen and ferricrocin, two ferric hydroxamate siderophores. J Am Chem Soc 105:810–815