Efficient plant regeneration of Malaysian indica rice MR 219 and 232 via somatic embryogenesis system
Tóm tắt
Improvement on rice plant regeneration system from an embryogenic callus using two Malaysian indica rice MR 219 and MR 232 was developed in this study. Different stages of rice explants (zygotic embryos) were tested for callus induction and regeneration using various carbon sources and amino acids. The present study shows that dough stage of zygotic embryos was the best stage of explants for the embryogenic callus induction and regeneration of both rice cultivars. The embryogenic callus nature with the compact structure was confirmed by scanning electron microscopy (SEM) analysis. Inclusion of maltose at the concentration between 10 and 20 mg/L shown higher responsive for the development of green somatic embryos and initiation of rice regeneration with an average of 29–37 (87–91%) regenerated plantlets, respectively. All in vitro regenerated rice plantlets were green, morphological and physiologically healthy condition. Rice plantlets were hardened in acclimatization room for 3 weeks and later transferred into soil with 95% survival in both varieties were recorded. Hence, regeneration system from zygotic rice embryos via somatic embryogenesis system was carried out successfully by using 10 g/L of maltose and combinations of glutamine, asparagine and arginine amino acids.
Tài liệu tham khảo
Datta SK (2004) Rice Biotechnology—a need for developing countries. AgBio Forum 7:31
Geng P, La H, Wang H, Stevens EJC (2008) Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.). Plant Cell Tiss Org Cult 92:303–313
Gnanapragasam S, Vasil IK (1992) Cryopreservation of immature embryos, embryogenic callus and cell suspension cultures of gramineous species. Plant Sci 83:205–215
Islam MM, Wahed SA, Khan SAKU (2004) Studies on callus induction and regeneration from dehusked rice (Oryza sativa L.) seeds. Plant Tiss Cult 14(2):151–160
Islam MM, Ahmed M, Mahaldar D (2005) In vitro callus induction and plant regeneration in seed explants of rice (Oryza sativa L.). Res J Agri Biol Sci 1(1):72–75
Khaleda L, Al-Forkan M (2006) Genotypic variability in callus induction and plant regeneration through somatic embryogenesis of five deepwater rice (Oryza sativa L.) cultivars of Bangladesh. Afr J Biotechnol 5(16):1435–1440
Lee KS, Jeon HS, Kim MY (2002) Optimization of a mature embryos-based in vitro culture system for high-frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivar. Plant Cell Tiss Org Cult 71:9–13
Li L, Qu R, de Kochko A, Fauquet C, Beachy RN (1993) An improved rice transformation system using the biolistic method. Plant Cell Rep 12:250–255
Morrish F, Vasil V, Vasil IL (1987) Development of morphogenesis and genetic manipulation in tissue and cell cultures of the gramineae. Adv Gen 24:432–499
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–479
Naqvi SMS, Sultana T, Yasmin T, Mahmood T, Akhtar MH (2006) Efficient embryogenic system from tissue culture of mature embryos for some coarse varieties of rice (Oryza sativa L.). Pak J Bot 38(4):969–975
Ramesh M, Murugiah V, Gupta AK (2009) Efficient in vitro plant regeneration via leaf base segments of indica rice (Oryza sativa L.). Ind J Exp Biol 47:68–74
Saharan V, Yadav RC, Yadav NR, Chapagain BP (2004) High frequency plant regeneration from desiccated calli of indica rice (Oryza sativa L.). Afr J Biotechnol 3(5):256–259
Sivamani E, Shen P, Opalka N, Beachy RN, Fauquet CM (1996) Selection of large quantities of embryogenic calli from indica rice seeds for production of fertile transgenic plants using the biolistic method. Plant Cell Rep 15:322–327
Syaiful BP, Siti NAA, Maheran AA, Sariah M, Othman O (2009) Somatic embryogenesis from scutellar embryo of Oryza sativa L. var. MR 219. Pertanika J Trop Agri Sci 32(2):185–194
Taylor PWJ, Ko HL, Adkins SW, Rathus S, Birch RG (1992) Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell Tiss Org Cult 28:69–78
Vega R, Vasquez N, Espinoza AM, Gatica AM, Melara MV (2009) Histology of somatic embryogenesis in rice (Oryza sativa cv. 5272). Rev Biol Trop 57(1):141–150
Zuraida AR, Suri R, Wan Zaliha WS, Sreeramanan S (2010) Regeneration of Malaysian indica rice (Oryza sativa L.) variety MR 232 via optimised somatic embryogenesis system. J Phytol 2(3):30–38