Salinity tolerance of Aegilops cylindrica genotypes collected from hyper-saline shores of Uremia Salt Lake using physiological traits and SSR markers
Tóm tắt
Uremia Salt Lake,
in North West Iran, has a hyper-saline water. A rare highly salinity-tolerant grass species, Aegilops cylindrica grows along its shores. Salinity tolerance of 44 genotypes of Ae. cylindrica, mainly collected from the Lake, was evaluated under control and 400 mM NaCl conditions using the physiological traits of plant height, dry weight, proline content, Na+ and K+ concentrations as well as K+/Na+ ratio. To evaluate the association between microsatellite (EST-SSR and SSR) markers and salinity tolerance, 35 primer pairs were used. Results showed a significant variation in the 44 genotypes studied in terms of their traits except for proline content. Ten most salinity-tolerant genotypes were identified based on their ability to survive, to produce the highest dry weight, and to sustain the least leaf Na+ concentration under salinity stress. The very high negative correlation found between Na+ concentration and salinity tolerance revealed the importance of individual or a combination of Na+ exclusion and excretion mechanisms contributing to the hyper-salinity tolerance of these genotypes. Clustering analysis based on marker data divided the 44 studied genotypes into two groups that were consistent with their saline and non-saline geographical areas. Results of molecular markers showed that four microsatellite markers (Xgwm312, Xwmc170, Xgwm291 and Xgwm410) generated a distinguished banding pattern in ten most salinity-tolerant genotypes. These results supported previous reports on their linkage with Na+ exclusion genes (HKT1;5 and HKT1;4) in wheat, which provided further evidence of usefulness of both genes and the linked markers to the salinity tolerance of the halophytic grass family species.
Tài liệu tham khảo
Ahmad M, Shahzad A, Iqbal M, Asif M, Hiran AH (2013) Morphological and molecular genetic variation in wheat for salinity tolerance at germination and early seedling stage. Aust J Crop Sci 7:66–74
Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
Badaeva ED, Amosova AV, Muvavenko OV, Samatadze TE (2002) Genome differentiation in Aegilops. 3. Evolution of the D-genome cluster. Plant Syst Evol 231:163–190
Bates LS, Waldern RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207
Bordbar F, Rahiminejad MR, Saeidi H, Blattner FR (2011) Phylogeny and genetic diversity of D-genome species of Aegilops and Triticum (Triticeae, Poaceae) from Iran based on microsatellites, ITS, and trnL-F. Plant Syst Evol 291:117–131
Byrt CS, Platten JD, Spielmeyer W, James RA, Lagudah ES, Dennis ES, Tester M, Munns R (2007) HKT1;5-like cation transporters linked to Na+ exclusion loci in wheat, Nax2 and Kna1. Plant Physiol 143:1918–1928
Colmer TD, Epstein E, Dvorak J (1995) Differential solute regulation in leaf blades of various ages in salt sensitive wheat and a salt-tolerant wheat × Lophopyrum elongatum (Host) A. Love amphiploid. Plant Physiol 108:1715–1724
Colmer TD, Flowers TJ, Munns R (2006) Use of wild relatives to improve salt tolerance in wheat. J Exp Bot 57:1059–1078
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Dubcovsky J, Maria GS, Epstein E, Luo MC, Dvorak J (1996) Mapping of the K+/Na+ discrimination locus Kna1 in wheat. Theor Appl Genet 92:448–454
Eimanifar A, Mohebbi F (2007) Urmia Lake (Northwest Iran): a brief review. Saline Syst 3:1–8
Farooq S, Niazi M, Iqbal N, Shah TM (1989) Salt tolerance potential of wild resources of the tribe Triticeae II. Screening of species of the genus Aegilops. Plant Soil 119:255–260
Flowers TJ, Colmer TD (2008) Salinity tolerance in halophytes. New Phytol 179:945–963
Gandhi HT, Vales MI, Watson CJW, Mallory-Smith CA, Mori N, Rehman M, Zemetra RS, Riera-Lizarazu O (2005) Chloroplast and nuclear microsatellite analysis of Aegilops cylindrica. Theor Appl Genet 111:561–572
Gandhi HT, Vales MI, Mallory-Smith C, Riera-Lizarazu O (2009) Genetic structure of Aegilops cylindrica host in its native range and in the United States of America. Theor Appl Genet 119:1013–1025
Garthwaite AJ, Bothmer RV, Colmer TD (2005) Salt tolerance in wild Hordeum species is associated with restricted entry of Na+ and Cl− into the shoots. J Exp Bot 56:2365–2378
Ghassemi F, Jakeman AJ, Nix HA (1995) Salinisation of land and water resources: human causes, extent and management and case studies. In: Centre for resource and environmental studies. The Australian National University, Canberra
Gorham J, Hardy C, Wyn Jones RG, Joppa LR, Law CN (1987) Chromosomal location of a K+/Na+ discrimination character in the D genome of wheat. Theor Appl Genet 74:584–588
Gorham J, Wyn Jones RG, Bristol A (1990) Partial characterization of the trait for enhanced K+/Na+ discrimination in the D genome of wheat. Planta 180:590–597
Hoagland DR, Arnon DI (1950) The water culture method for growing plants without soil. Calif Agric Exp Stn Circ 347:32p
James RA, Blake C, Byrt CS, Munns R (2011) Major genes for Na+ exclusion, Nax1 and Nax2 (wheat HKT1;4 and HKT1;5), decrease Na+ accumulation in bread wheat leaves under saline and waterlogged conditions. J Exp Bot 62:2939–2947
Leonova IN, Roder MS, Nasyrova F (2009) The application of wheat microsatellite markers for the detection of interspecific variation in tetraploid Aegilops species with C and U genomes. Cereal Res Commun 37:335–343
Li-Fang Z, Li-Xiao S, Yi-Gao F, Bao-Li Q, Hai-Bin X, Zi-You P, Zeng-Jun Q (2008) Development and chromosome mapping of new wheat EST-SSR markers and application for characterizing rye chromosomes added in wheat. Acta Agron Sin 34:926–933
Lindsay P, Lagudah S, Hare A, Munns R (2004) A locus for sodium exclusion (Nax1), a trait for salt tolerance, mapped in durum wheat. Funct Plant Biol 31:1105–1114
Moghaieb EA, Abdel-Hadi A, Talaat B (2011) Molecular markers associated with salt tolerance in Egyptian wheats. Afr J Biotechnol 79:18092–18103
Munns R, James RA (2003) Screening methods for salinity tolerance: a case study with tetraploid wheat. Plant Soil 253:201–218
Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681
Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323
Poustini K, Siosemardeh A (2004) Ion distribution in wheat cultivars in response to salinity stress. Field Crops Res 85:125–133
Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Rohlf FJ (2002) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.11a. Applied Biostatistics, New York
SAS Institute (2002) SAS version 9.1. SAS Institute. Cary, North Carolina
Sawahel WA, Hassan AH (2002) Generation of transgenic wheat plants producing high levels of the osmoprotectant proline. Biotechnol Lett 24:721–725
Shahzad A, Ahmad M, Iqbal M, Ahmed I, Ali GM (2012) Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers. Genet Mol Res 11:679–692
Shavrukov Y, Langridge P, Tester M (2009) Salinity tolerance and sodium exclusion in genus Triticum. Breed Sci 59:671–678
Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–537
Xu ZL, Ali Z, Yi JX, He XL, Zhang DY, Yu GH, Khan AA, Khan IA, Ma HX (2011) Expressed sequence tag-simple sequence repeat-based molecular variance in two Salicornia (Amaranthaceae) populations. Genet Mol Res 10:1262–1276
Xu SC, Gong YM, Mao WH, Hu QZ, Zhang GW, Fu W, Xian QQ (2012) Development and characterization of 41 novel EST-SSR markers for Pisum sativum (Leguminosea). Am J Bot 99:149–153