A novel association between hemoglobin subunit beta gene and reproductive performance in Awassi ewes

AbdulAzeez, 2016, In-silico computing of the most deleterious nsSNPs in HBA1 gene, PloS one, 11, 10.1371/journal.pone.0147702 Abdullah, 2002, Protocols for estrus synchronization in Awassi ewes under arid conditions, Asian Austral. J. Anim. Sci., 15, 957, 10.5713/ajas.2002.957 Akinyemi, 2010, Haemoglobin polymorphism and morphometric correlates in the West African Dwarf sheep of Nigeria, Int. J. Morphol., 28, 205, 10.4067/S0717-95022010000100029 Alanazi, 2011, In silico analysis of single nucleotide polymorphism (SNPs) in human β-globin gene, PloS one, 6, 10.1371/journal.pone.0025876 Alenzi, 2019, Biochemical and molecular analysis of the beta-globin gene on Saudi sickle cell anemia, Saudi J. Biol. Sci., 26, 1377, 10.1016/j.sjbs.2019.03.003 Alkass, J.E., Juma, K.H., 2005. Small ruminant breeds of Iraq. In: Iñiguez, L., Ed. Characterization of Small Ruminant Breeds in West Asia, North Africa, Vol. 1, West Asia. ICARDA, Aleppo, Syria. P. 63–101. Al-Khuzai, 2019, Relationship of hemoglobin types and blood groups with bodyweight and dimensions in unimproved awassi ewes, Adv. Anim. Vet. Sci., 7, 461, 10.17582/journal.aavs/2019/7.6.461.465 Al-Saaidi, 2018, Reproductive fecundity of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid, Asian Austral. J. Anim. Sci., 31, 1169, 10.5713/ajas.17.0660 Al-Shuhaib, 2017, A Universal, rapid, and inexpensive method for genomic DNA isolation from the whole blood of mammals and birds, J. Genet., 96, 171, 10.1007/s12041-017-0750-6 Al-Shuhaib, 2018, Highly deleterious variations in COX1, CYTB, SCG5, FK2, PRL and PGF genes are the potential adaptation of the immigrated African ostrich population, Comput. Biol. Med., 100, 17, 10.1016/j.compbiomed.2018.06.019 Al-Shuhaib, 2019, A comprehensive in silico prediction of the most deleterious missense variants in the bovine LEP gene, Biotechnol., 100, 429, 10.5114/bta.2019.90244 Al-Shuhaib, 2019, GHRL gene-based genotyping of ovine and caprine breeds reveals highly polymorphic intronic sequences in Awassi sheep with several RNA motifs, J. Genet. Eng. Biotechnol., 17, 3, 10.1186/s43141-019-0004-5 Bettati, 2009, Hemoglobin, an “evergreen” red protein, Biochim. Biophys. Acta., 1794, 1317, 10.1016/j.bbapap.2009.03.020 Bezova, 2007, Analysis of genetic polymorphism of blood proteins and selected meat quality traits in rabbits, Slovak J. Anim. Sci., 40, 57 Boonprong, 2007, Relationship between haemoglobin types and productivity of Thai indigenous and Simmental x Brahman crossbred cattle, Livest. Sci., 111, 213, 10.1016/j.livsci.2007.01.149 Botstein, 1980, Construction of a genetic linkage map in man using restriction fragment length polymorphisms, Am. J. Hum. Genet., 32, 314 Brown, 2015, Hemoglobin: a gas transport molecule that is hormonally regulated in the ovarian follicle in mice and humans, Biol. Reprod., 92, 26, 10.1095/biolreprod.114.124594 Byun, 2009, An effective method for silver-staining DNA in large numbers of polyacrylamide gels, Anal. Biochem., 385, 174, 10.1016/j.ab.2008.10.024 Capriotti, 2005, I-Mutant 2.0: predicting stability changes upon mutation from the protein sequence or structure, Nucl. Acids Res., 33, W306, 10.1093/nar/gki375 Choi, 2012, Predicting the functional effect of amino acid substitutions and indels, Plos one, 7, 10.1371/journal.pone.0046688 Egena, S.S.A., Alao, R.A., 2014. Haemoglobin polymorphism in selected farm animals - A review. Biotech. Anim. Husbandry 30(3), 377–390, 2014. doi: 10.2298/BAH1403377E Gasteiger, E., Gattiker, A., Hoogland, C., Ivanyi, I., Appel, R.D., Bairoch, A., 2003. ExPASy: the proteomics server for in-depth protein knowledge and analysis. Nucleic Acids Res. 31, 3784–3788. doi: 10.1093/nar/gkg563 Gwaza, 2019, Assessment of haemoglobin polymorphism as a potential protein marker in selection for genetic improvement of the West African Dwarf Goat population in Nigeria, Int. J. Biotechnol., 8, 38, 10.18488/journal.57.2019.81.38.47 Hashimoto, 2000, Low oxygen tension during in vitro maturation is beneficial for supporting the subsequent development of bovine cumulus-oocyte complexes, Mol. Reprod. Develop.: Incorporating Gamete Res., 57, 353, 10.1002/1098-2795(200012)57:4<353::AID-MRD7>3.0.CO;2-R Inusa, 2019, Sickle cell disease—genetics, pathophysiology, clinical presentation and treatment, International Journal of Neonatal Screening, 5, 20, 10.3390/ijns5020020 Kelley, 2015, The Phyre2 web portal for protein modeling, prediction and analysis, Nat. Protoc., 10, 845, 10.1038/nprot.2015.053 Ng, 2006, Predicting the effects of amino acid substitutions on protein function, Annu. Rev. Genom. Hum. Genet., 22, 61, 10.1146/annurev.genom.7.080505.115630 Nihat, 2003, Correlation between biochemical parameters and production traits in Merino crosses sheep. Haemoglobin and transferrin types, Turk J. Vet. Anim. Sci., 27, 575 Pieragostini, 2010, Insights into Hemoglobin Polymorphism and Related Functional Effects on Hematological Pattern in Mediterranean Cattle, Goat and Sheep, Diversity, 2, 679, 10.3390/d2040679 Pieragostini, 2006, Functional effect of hemoglobin polymorphism on the hematological pattern of Gentile di Puglia sheep, J. Anim. Breed. Genet., 123, 122, 10.1111/j.1439-0388.2006.00566.x Pires, 2014, DUET: a server for predicting effects of mutations on protein stability using an integrated computational approach, Nucleic Acids Res., 42, W314, 10.1093/nar/gku411 Pires, 2012, mCSM: predicting the effects of mutations in proteins using graph-based signatures, Bioinformatics, 30, 335, 10.1093/bioinformatics/btt691 Pokharel, 2018, Integrated ovarian mRNA and miRNA transcriptome profiling characterizes the genetic basis of prolificacy traits in sheep (Ovis aries), BMC Genomics, 19, 104, 10.1186/s12864-017-4400-4 Rodrigues, 2018, DynaMut: Predicting the impact of mutations on protein conformation, flexibility and stability, Nucleic Acids Res., 46, W350, 10.1093/nar/gky300 Talafha, 2011, Awassi sheep reproduction and milk production: review, Trop. Anim. Health Prod., 43, 1316, 10.1007/s11250-011-9858-5 Tang, 2016, PANTHER-PSEP: predicting disease-causing genetic variants using position-specific evolutionary preservation, Bioinformatics, 32, 2230, 10.1093/bioinformatics/btw222 Üstüner, 2013, Main productive performance of Awassi sheep in the Central Anatolian region of Turkey, Turk. J. Vet. Anim. Sci., 37, 271 Važić, 2017, Relationship between the genetic hemoglobin polymorphism, morphometry and fertility of Pramenka sheep breed from Central Bosnia, Genetika, 49, 151, 10.2298/GENSR1701151V Vaughn, S.E., 2012. Review of the third edition of the Guide for the Care and Use of Agricultural Animals in Research and Teaching.J. Am. Assoc. Lab. Anim. Sci. 51(3), 298–300. Worth, 2012, SDM-a server for predicting effects of mutations on protein stability and malfunction, Nucleic Acids Res., 39, W215, 10.1093/nar/gkr363 Yates, 2014, SuSPect: enhanced prediction of single amino acid variant (SAV) phenotype using network features, J. Mol. Biol., 426, 2692, 10.1016/j.jmb.2014.04.026 Ye, 2012, Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction, BMC Bioinformatics, 13, 134, 10.1186/1471-2105-13-134 Yeh, 1999