Neutral red uptake assay for the estimation of cell viability/cytotoxicity
Tóm tắt
Từ khóa
Tài liệu tham khảo
Borenfreund, E. & Puerner, J.A. A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR90). J. Tissue Cult. Methods 9, 7–9 (1984).
Babich, H. & Borenfreund, E. Applications of the neutral red cytotoxicity assay to in vitro toxicology. Altern. Lab. Anim. 18, 129–144 (1990).
Clothier, R. The FRAME modified neutral red uptake cytotoxicity test. Protocol INVITTOX no.: 3 (1990). DB-ALM, ECVAM database service on Alternative Methods to Animal Experimentation. http://ecvam-dbalm.jrc.cec.eu.int/ .
Cavanaugh, P.F. et al. A semi-automated neutral red based chemosensitivity assay for drug screening. Invest. New Drugs 8, 347–354 (1990).
Spielmann, H. & Liebsch, M. BALB/C 3T3 cytotoxicity test protocol INVITTOX no.: 46 (1992). DB-ALM, ECVAM database service on Alternative Methods to Animal Experimentation. http://ecvam-dbalm.jrc.cec.eu.int/ .
Repetto, G. & Sanz, P. Neutral red uptake, cellular growth and lysosomal function: in vitro effects of 24 metals. Altern. Lab. Anim. 21, 501–507 (1993).
Commission Directive 2000/33/EC of 25 April 2000 adapting to technical progress for the 27th time Council Directive 67/548/EEC on the approximation of laws, regulations and administrative provisions relating to the classification, packaging and labelling of dangerous substances (Text with EEA relevance.) B. 41. Phototoxicity—in vitro 3T3 NRU phototoxicity test. OJ L 136, 8.6.2000, 90–107.
Organisation for Economic Co-operation and Development. Guideline 432 in vitro 3T3 nru phototoxicity test (original guideline, adopted 13th April 2004). OECD guidelines for the testing of chemicals, PDF edition (ISSN 1607-310X). http://caliban.sourceoecd.org/vl=8942027/cl=11/nw=1/rpsv/ periodical/p15_about.htm?jnlissn=1607310x .
Parish, C.R. & Mullbacher, A. Automated colorimetric assay for T cell cytotoxicity. J. Immunol. Methods 58, 223–237 (1983).
Winckler, J. Vital staining of lysosomes and other cell organelles of the rat with neutral Red. Prog. Histochem. Cytochem. 6, 1–89 (1974).
Nemes, Z., Dietz, R. & Luth, J.B. The pharmacological relevance of vital staining with neutral red. Experientia 35, 1475–1476 (1979).
Filman, D.J., Brawn, R.J. & Dankler, W.B. Intracellular supravital stain delocalization as an assay for antibody-dependent complement-mediated cell damage. J. Immunol Methods 6, 189–207 (1975).
Bitensky, L. The reversible activation of lysosomes in normal cells and the effect of pathological conditions. In Lysosomes (eds. de Reuck, A.V.S. & Cameron, M.P.) 362–375 (Little, Brown, Boston, 1963).
Fernández, M., Ríos, J.C., Jos, A. & Repetto, G. Comparative cytotoxicity of alachlor on RTG-2 trout and SH-SY5Y human cells. Arch. Environ. Contam. Toxicol. 51, 515–520 (2006).
Löwik, C.W.G.M., Alblas, M.J., van de Ruit, M. & Papapoulos, S.E. van der Pluijm, G. Quantification of adherent and non-adherent cells cultured in 96-well plates using the supravital satín neutral red. Anal. Biochem. 213, 426–433 (1993).
Borenfreund, E., Babich, H. & Martin-Alguacil, N. Comparisons of two in vitro cytotoxicity assays. The neutral red (NR) and tetrazolium MTT test. Toxicol. In Vitro 2, 1–6 (1988).
Vichai, V. & Kirtikara, K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat. Protoc. 1, 1112–1116 (2006).
Arranz, M.J. & Festing, M.F.W. Prior use of the neutral red assay and reduction of total protein determination in 96-well plate assays. Toxicol. In Vitro 4, 211–212 (1990).