Determination of particulate fatty acids as P-bromophenacyl- or phenylphenacylesters using HPLC

Springer Science and Business Media LLC - Tập 23 - Trang 419-422 - 1987
C. Osterroht1
1Institut für Meereskunde, Universität Kiel, Kiel 1, FRG

Tóm tắt

A method for the determination of fatty acids of the molecular weight range of propionic to erucic acid is reported. The acids are converted to either p-bromophenacyl-or p-phenylphenacylesters and the esters are separated by HPLC and detected by UV-absorption. Twentytwo esters, including saturated, monoolefinic and polyunsaturated ones were separated within one HPLC-run. The detection limit is about 5 pmol per fatty acid. The application of the method to particulate material is described.

Tài liệu tham khảo

B. J. B. Wood, Chapter 8 in: Algal Physiology and Biochemistry. (ed. W. D. P. Stewart), Blackwell Scientific Publications, oxford, London, Edinburgh, Melbourne (1974). D. M. Schultz, J. G. Quinn, J. Fish. Res. Board Can.29, 1482–1486 (1972). G. Kattner, U. H. Brockmann, Mar. Chem.6, 233–241 (1978). G. Kattner, G. Gercken, K. Eberlein, Mar. Chem.14, 149–162 (1983). H. J. W. De Baar, J. W. Farrington, S. G. Wakeham, J. Mar. Res.41, 19–41 (1983). M. J. Barcelona, H. M. Liljestrand, J. J. Morgan, Anal. Chem.52, 321–325 (1980). H. D. Durst, M. Milano, E. J. Kikta Jr., S. A. Connelly, E. Grushka, Anal. Chem.47, 1797–1801 (1975). E. O. Umeh, J. Chromatogr.56, 29–36 (1971). Y. L'Emeillat, J. F. Ménez, F. Berthou, L. Bardou, J. Chromatogr.206, 89–100 (1981). M. Larsson, C. Roos, Chromatographia17, 185–190 (1983). R. L. Patience, J. D. Thomas, J. Chromatogr.234, 225–230 (1982). H. D. Durst, Tetrahedron Lett. (28), 2421 (1974). C. L. Liotta, H. P. Harris, M. McDermott, T. Gonzales, K. Smith, Tetrahedron Lett. (28), 2417 (1974).