Measures of gene expression in sputum cells can identify TH2-high and TH2-low subtypes of asthma

Robinson, 1992, Predominant TH2-like bronchoalveolar T-lymphocyte population in atopic asthma, N Engl J Med, 326, 298, 10.1056/NEJM199201303260504 Corren, 2010, A randomized, controlled, phase 2 study of AMG 317, an IL-4Ralpha antagonist, in patients with asthma, Am J Respir Crit Care Med, 181, 788, 10.1164/rccm.200909-1448OC Kips, 2003, Effect of SCH55700, a humanized anti-human interleukin-5 antibody, in severe persistent asthma: a pilot study, Am J Respir Crit Care Med, 167, 1655, 10.1164/rccm.200206-525OC Nair, 2009, Mepolizumab for prednisone-dependent asthma with sputum eosinophilia, N Engl J Med, 360, 985, 10.1056/NEJMoa0805435 Woodruff, 2007, Genome-wide profiling identifies epithelial cell genes associated with asthma and with treatment response to corticosteroids, Proc Natl Acad Sci U S A, 104, 15858, 10.1073/pnas.0707413104 Woodruff, 2009, T-helper type 2-driven inflammation defines major subphenotypes of asthma, Am J Respir Crit Care Med, 180, 388, 10.1164/rccm.200903-0392OC Truyen, 2006, Evaluation of airway inflammation by quantitative Th1/Th2 cytokine mRNA measurement in sputum of asthma patients, Thorax, 61, 202, 10.1136/thx.2005.052399 2005, ATS/ERS recommendations for standardized procedures for the online and offline measurement of exhaled lower respiratory nitric oxide and nasal nitric oxide, 2005, Am J Respir Crit Care Med, 171, 912, 10.1164/rccm.200406-710ST Gershman, 1996, Comparison of two methods of collecting induced sputum in asthmatic subjects, Eur Respir J, 9, 2448, 10.1183/09031936.96.09122448 Schroeder, 2006, The RIN: an RNA integrity number for assigning integrity values to RNA measurements, BMC Mol Biol, 7, 3, 10.1186/1471-2199-7-3 Fleige, 2006, RNA integrity and the effect on the real-time qRT-PCR performance, Mol Aspects Med, 27, 126, 10.1016/j.mam.2005.12.003 Fleige, 2006, Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR, Biotechnol Lett, 28, 1601, 10.1007/s10529-006-9127-2 Dougherty, 2010, Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma, J Allergy Clin Immunol, 125, 1046, 10.1016/j.jaci.2010.03.003 McGrath, 2012, A large subgroup of mild-to-moderate asthma is persistently noneosinophilic, Am J Respir Crit Care Med, 185, 612, 10.1164/rccm.201109-1640OC Jia, 2012, Periostin is a systemic biomarker of eosinophilic airway inflammation in asthmatic patients, J Allergy Clin Immunol, 130, 647, 10.1016/j.jaci.2012.06.025 Baines, 2011, Transcriptional phenotypes of asthma defined by gene expression profiling of induced sputum samples, J Allergy Clin Immunol, 127, 153, 10.1016/j.jaci.2010.10.024 Abbas, 2005, Immune response in silico (IRIS): immune-specific genes identified from a compendium of microarray expression data, Genes Immun, 6, 319, 10.1038/sj.gene.6364173 Choy, 2011, Gene expression patterns of Th2 inflammation and intercellular communication in asthmatic airways, J Immunol, 186, 1861, 10.4049/jimmunol.1002568 Chakir, 2003, Airway remodeling-associated mediators in moderate to severe asthma: effect of steroids on TGF-beta, IL-11, IL-17, and type I and type III collagen expression, J Allergy Clin Immunol, 111, 1293, 10.1067/mai.2003.1557 Al-Ramli, 2009, T(H)17-associated cytokines (IL-17A and IL-17F) in severe asthma, J Allergy Clin Immunol, 123, 1185, 10.1016/j.jaci.2009.02.024