Redox cycling compounds generate H2O2 in HTS buffers containing strong reducing reagents—real hits or promiscuous artifacts?

Inglese, 2007, High-throughput screening assays for the identification of chemical probes, Nat Chem Biol, 3, 466, 10.1038/nchembio.2007.17

Johnston, 2002, Cellular platforms for HTS: three case studies, Drug Discov Today, 7, 353, 10.1016/S1359-6446(01)02140-7

Brideau, 2003, Improved statistical methods for hit selection in high-throughput screening, J Biomol Screen, 8, 634, 10.1177/1087057103258285

Malo, 2006, Statistical practice in high-throughput screening data analysis, Nat Biotechnol, 24, 167, 10.1038/nbt1186

Baell, 2010, New substructure filters for removal of pan assay interference compounds (PAINS) from screening libraries and for their exclusion in bioassays, J Med Chem, 53, 2719, 10.1021/jm901137j

Thorne, 2010, Apparent activity in high-throughput screening: origins of compound-dependent assay interference, Curr Opin Chem Biol, 14, 315, 10.1016/j.cbpa.2010.03.020

Auld, 2008, Characterization of chemical libraries for luciferase inhibitory activity, J Med Chem, 51, 2372, 10.1021/jm701302v

Johnston, 2008, Development of a 384-well colorimetric assay to quantify hydrogen peroxide generated by the redox cycling of compounds in the presence of reducing agents, Assay Drug Dev Technol, 6, 505, 10.1089/adt.2008.151

Lor, 2007, A simple assay for detection of small-molecule redox activity, J Biomol Screen, 12, 881, 10.1177/1087057107304113

Soares, 2010, Profiling the NIH small molecule repository for compounds that generate H(2)O(2) by redox cycling in reducing environments, Assay Drug Dev Technol, 8, 152, 10.1089/adt.2009.0247

Simeonov, 2008, Fluorescence spectroscopic profiling of compound libraries, J Med Chem, 51

Thorne, 2010, Illuminating insights into firefly luciferase and other bioluminescent reporters used in chemical biology, Chem Biol, 17, 646, 10.1016/j.chembiol.2010.05.012

Bova, 2004, The oxidative mechanism of action of ortho-quinone inhibitors of protein-tyrosine phosphatase [alpha] is mediated by hydrogen peroxide, Arch Biochem Biophys, 429, 30, 10.1016/j.abb.2004.05.010

Smith, 2002, Expression, preparation, and high-throughput screening of caspase-8: discovery of redox-based and steroid diacid inhibition, Arch Biochem Biophys, 399, 195, 10.1006/abbi.2002.2757

Johnston, 2007, Development and implementation of a 384-well homogeneous fluorescence intensity high-throughput screening assay to identify mitogen-activated protein kinase phosphatase-1 dual-specificity protein phosphatase inhibitors, Assay Drug Dev Technol, 5, 319, 10.1089/adt.2007.066

Johnston, 2009, Characterization of the Cdc25B dual specificity phosphatase inhibitor hits identified in a high throughput screen of the NIH compound library, Assay Drug Dev Technol, 7, 250, 10.1089/adt.2008.186

Brisson, 2005, Redox regulation of Cdc25B by cell-active quinolinediones, Mol Pharmacol, 68, 1810, 10.1124/mol.105.016360

Keinan, 2008, Computational design, synthesis and biological evaluation of para-quinone-based inhibitors for redox regulation of the dual-specificity phosphatase Cdc25B, Org Biomol Chem, 6, 3256, 10.1039/b806712k

Lal, 1997, Radical-induced oxidation of dithiothreitol in acidic oxygenated solution: a chain reaction, J Am Chem Soc, 119, 5735, 10.1021/ja970744l

Brown, 2009, Nox proteins in signal transduction, Free Radic Biol Med, 47, 1239, 10.1016/j.freeradbiomed.2009.07.023

Forman, 2010, Signaling functions of reactive oxygen species, Biochemistry, 49, 835, 10.1021/bi9020378

Rhee, 2005, Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins, Curr Opin Cell Biol, 17, 183, 10.1016/j.ceb.2005.02.004

Rhee, 2003, Cellular regulation by hydrogen peroxide, J Am Soc Nephrol, 14, S211, 10.1097/01.ASN.0000077404.45564.7E

Miller, 2010, Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling, Proc Natl Acad Sci USA, 107, 15681, 10.1073/pnas.1005776107

Ostman, 2006, Protein-tyrosine phosphatases and cancer, Nat Rev Cancer, 6, 307, 10.1038/nrc1837

Denu, 1998, Specific and reversible inactivation of protein tyrosine phosphatases by hydrogen peroxide: evidence for a sulfenic acid intermediate and implications for redox regulation, Biochemistry, 37, 5633, 10.1021/bi973035t

Forman, 2004, Redox signaling: thiol chemistry defines which reactive oxygen and nitrogen species can act as second messengers, Am J Physiol Cell Physiol, 287, C246, 10.1152/ajpcell.00516.2003

Lee, 2002, Reversible inactivation of the tumor suppressor PTEN by H2O2, J Biol Chem, 277, 20336, 10.1074/jbc.M111899200

Rhee, 2000, Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation, Sci STKE, 2000, PE1, 10.1126/stke.2000.53.pe1

Salmeen, 2003, Redox regulation of protein tyrosine phosphatase 1B involves a sulphenyl-amide intermediate, Nature, 423, 769, 10.1038/nature01680

Seth, 2006, Redox regulation of MAP kinase phosphatase 3, Biochemistry, 45, 8476, 10.1021/bi060157p

Lee, 2002, Inhibition of PTPs by H2O2 regulates the activation of distinct MAPK pathways, Free Radic Biol Med, 33, 1121, 10.1016/S0891-5849(02)01000-6

Rhee, 2006, Cell signaling, H2O2, a necessary evil for cell signaling, Science, 312, 1882, 10.1126/science.1130481

Rhee, 2005, Intracellular messenger function of hydrogen peroxide and its regulation by peroxiredoxins, Curr Opin Cell Biol, 17, 183, 10.1016/j.ceb.2005.02.004

Bembenek, 2005, A fluorescence-based coupling reaction for monitoring the activity of recombinant human NAD synthetase, Assay Drug Dev Technol, 3, 533, 10.1089/adt.2005.3.533

Riss, 2004, Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays, Assay Drug Dev Technol, 2, 51, 10.1089/154065804322966315

Pick, 1980, A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture, J Immunol Methods, 38, 161, 10.1016/0022-1759(80)90340-3

Bolton, 2000, Role of quinones in toxicology, Chem Res Toxicol, 13, 135, 10.1021/tx9902082

Goetz, 2008, Reactive species: a cell damaging rout assisting to chemical carcinogens, Cancer Lett, 266, 73, 10.1016/j.canlet.2008.02.035

Villamil, 2004, Redox cycling of -lapachone and structural analogues in microsomal and cytosol liver preparations, Methods Enzymol, 378, 67, 10.1016/S0076-6879(04)78004-0

Auld, 2008, Literature search and review, Assay Drug Dev Technol, 6, 621, 10.1089/adt.2008.9986

Yi, 2008, A novel class of small molecule inhibitors of Hsp90, ACS Chem Biol, 3, 645, 10.1021/cb800162x

Yi, 2009, An AlphaScreen-based high-throughput screen to identify inhibitors of Hsp90-cochaperone interaction, J Biomol Screen, 14, 273, 10.1177/1087057108330114