Chemical features of Ganoderma polysaccharides with antioxidant, antitumor and antimicrobial activities

Phytochemistry - Tập 114 - Trang 38-55 - 2015
Isabel C.F.R. Ferreira1, Sandrina A. Heleno1,2, Filipa S. Reis1,3, Dejan Stojkovic4, Maria João R.P. Queiroz2, M. Helena Vasconcelos3, Marina Sokovic4
1Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Portugal
2Centre of Chemistry, University of Minho, Braga, Portugal
3Cancer Drug Resistance Group, IPATIMUP, Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal
4University of Belgrade, Institute for Biological Research “Siniša Stanković”, Department of Plant Physiology, Belgrade, Serbia

Tài liệu tham khảo

Amaral, 2008, An unusual water-soluble β-glucan from the basidiocarp of the fungus Ganoderma resinaceum, Carbohydr. Polym., 72, 473, 10.1016/j.carbpol.2007.09.016 Bai, 2008, Antiblastic activity of Ganoderma lucidum polysaccharides, Acta Agric. Bor. Sin., S1 Bao, 2002, Structural features of immunologically active polysaccharides from Ganoderma lucidum, Phytochemistry, 59, 175, 10.1016/S0031-9422(01)00450-2 Bhattacharyya, 2006, Antimicrobial activities of some Basidiomycetous fungi, J. Mycopathol. Res., 44, 129 Cao, 2004, Antitumor and anti-angiogenic activity of Ganoderma lucidum polysaccharides peptide, Acta Pharmacol. Sin., 25, 833 Cao, 2006, Ganoderma lucidum polysaccharides peptide inhibits the growth of vascular endothelial cell and the induction of VEGF in human lung cancer cell, Life Sci., 78, 1457, 10.1016/j.lfs.2005.07.017 Chen, 1998, Chemical structure of the water-insoluble polysaccharide isolated from the fruiting body of Ganoderma lucidum, Polym. J., 30, 838, 10.1295/polymj.30.838 Chen, 2008, Purification, composition analysis and antioxidant activity of a polysaccharide from the fruiting bodies of Ganoderma atrum, Food Chem., 107, 231, 10.1016/j.foodchem.2007.08.021 Cho, 1999, Chemical features and purification of immunostimulating polysaccharides from the fruit bodies of Agaricus blazei, Korean J. Mycol., 27, 170 Dong, 2012, A novel water-soluble β-D-glucan isolated from the spores of Ganoderma lucidum, Carbohydr. Res., 353, 100, 10.1016/j.carres.2012.02.029 Ferreira, 2010, Compounds from wild mushrooms with antitumor potential, Anti-cancer Agents Med. Chem., 10, 424, 10.2174/1871520611009050424 Gao, 1996, Characterization of acidic heteroglycans from Tremella fuciformis Berk with cytokine stimulating activity, Carbohydr. Res., 288, 135, 10.1016/S0008-6215(96)90789-2 Gao, 2003, Antibacterial and antiviral value of the genus Ganoderma P. Karst species (Aphyllophoromycetideae): a review, Int. J. Med. Mushr., 5, 235, 10.1615/InterJMedicMush.v5.i3.20 Gao, 2003, Effects of ganopolys (a Ganoderma lucidum polysaccharide extract) on the immune functions in advanced-stage cancer patients, Immunol. Investigations, 32, 201, 10.1081/IMM-120022979 Gao, 2004, Chemopreventive and tumoricidal properties of Lingzhi mushroom Ganoderma lucidum (W. Curt.:Fr.) Lloyd (Aphyllophorophoromycetideae). I. Preclinical and clinical studies (review), Int. J. Med. Mushr., 6, 95, 10.1615/IntJMedMushr.v6.i2.10 Gao, 2005, Antitumor activity and underlying mechanisms of Ganopoly, the refined polysaccharides extracted from Ganoderma lucidum, in mice, Immunol. Investigations, 34, 171, 10.1081/IMM-55813 Gao, 2005, Effects of water-soluble Ganoderma lucidum polysaccharides on the immune functions of patients with advanced lung cancer, J. Med. Food., 8, 159, 10.1089/jmf.2005.8.159 Haghi, 2011 Heleno, 2012, Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: a comparative study of the antioxidant potential of phenolic and polysaccharidic extracts, Food Res. Int., 46, 135, 10.1016/j.foodres.2011.12.009 Heleno, 2013, Antimicrobial and demelanizing activity of Ganoderma lucidum extract, p-hydroxybenzoic and cinnamic acids and their synthetic acetylated glucuronide methyl esters, Food Chem. Toxicol., 58, 95, 10.1016/j.fct.2013.04.025 Huang, 2007, Study on microwave-assisted extraction of polysaccharides from spores of Ganoderma atrum with response surface analysis, Food Sci., 28, 200 Huang, 2010, Extraction of polysaccharide from Ganoderma lucidum and its immune enhancement activity, Int. J. Biol. Macromol., 47, 336, 10.1016/j.ijbiomac.2010.03.019 Huang, 2011, Purification and structural characterization of a new water-soluble neutral polysaccharide GLP-F1-1 from Ganoderma lucidum, Int. J. Biol. Macromol., 48, 165, 10.1016/j.ijbiomac.2010.10.015 Huie, 2004, Chromatographic and electrophoretic methods for Lingzhi pharmacologically active components, J. Chromatogr., 812, 241 Jia, 2009, Evaluation of in vivo antioxidant activities of Ganoderma lucidum polysaccharides in STZ-diabetic rats, Food Chem., 115, 32, 10.1016/j.foodchem.2008.11.043 Jiang, 2012, Rapid purification of polysaccharides using novel radial flow ion-exchange by response surface methodology from Ganoderma lucidum, Food Bioproducts Process., 90, 1, 10.1016/j.fbp.2010.12.001 Kao, 2011, Structural characterization and antioxidative activity of low-molecular-weights Beta-1,3-glucan from the residue of extracted Ganoderma lucidum fruiting bodies, J. Biomed. Biotechnol., 2012, 1, 10.1155/2012/673764 Kawagishi, 1990, Formulation of a potent antitumor (1→6)-beta-D-glucan-protein complex from Agaricus blazei fruiting bodies and antitumor activity of the resulting products, Carbohydr. Polym., 12, 393, 10.1016/0144-8617(90)90089-B Kim, 1993, Studies on constituents of higher fungi of Korea (LXVIII). Antitumor components of the cultured mycelia of Ganoderma lucidum, Korean J. Pharmacol., 24, 203 Kirk, 2011 Kozarski, 2011, Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus, Food Chem., 129, 1667, 10.1016/j.foodchem.2011.06.029 Kozarski, 2012, Antioxidative activities and chemical characterization of polysaccharide extracts from the widely used mushrooms Ganoderma applanatum, Ganoderma lucidum, Lentinus edodes and Trametes versicolor, J. Food Comp. Anal., 26, 144, 10.1016/j.jfca.2012.02.004 Lee, 2001, Inhibition of lipid peroxidation and oxidative damage by Ganoderma lucidum, Phytother. Res., 15, 245, 10.1002/ptr.830 Li, 2007, Inhibition of Lycium barbarium polysaccharides and Ganoderma lucidum polysaccharides against oxidative injury induced by γ-irradiation in rat liver mitochondria, Carbohydr. Polym., 69, 172, 10.1016/j.carbpol.2006.09.021 Li, 2008, Changes of serum interferon-gamma levels in mice bearing S-180 tumor and the interventional effect of immunomodulators, Nan Fang Yi Ke Da Xue Xue Bao, 28, 65 Li, 2009, The protective effect of Ganoderma atrum polysaccharide against anoxia/reoxygenation injury in neonatal rat cardiomyocytes, Life Sci., 85, 634, 10.1016/j.lfs.2009.09.001 Li, 2010, Ganoderma atrum polysaccharide protects cardiomyocytes against anoxia/reoxygenation-induced oxidative stress by mitochondrial pathway, J. Cell Biochem., 110, 191 Li, 2011, Ganoderma atrum polysaccharide attenuates oxidative stress induced by D-galactose in mouse brain, Life Sci., 88, 713, 10.1016/j.lfs.2011.02.010 Li, 2011, Enhancement of cyclophosphamide-induced antitumor effect by a novel polysaccharide from Ganoderma atrum in sarcoma 180-bearing mice, J. Agric. Food Chem., 59, 3707, 10.1021/jf1049497 Li, 2012, Antimicrobial properties, antioxidant activity and cytotoxicity of ethanol-soluble acidic components from Ganoderma atrum, Food Chem. Toxicol., 50, 689, 10.1016/j.fct.2011.12.011 Li, 2012, Ganoderma atrum polysaccharide improves age-related oxidative stress and immune impairment in mice, J. Agric Food Chem., 60, 1413, 10.1021/jf204748a Liang, 1994, Isolation, purification and structural comparison investigation of water soluble polysaccharide GFb of fermented mycelia from Ganoderma tsugae, Acta Mycol. Sin., 13, 211 Liao, 2013, Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes, Proc. Natl. Acad. Sci. U.S.A., 110, 13809, 10.1073/pnas.1312457110 Lin, 1999, Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms, Acta Pharmacol. Sin., 25, 1387 Lindequist, 2005, The pharmacological potential of mushrooms, Evid. Based Complement Alternat. Med., 2, 285, 10.1093/ecam/neh107 Liu, 2002, Antitumor activity of the sporoderm-broken germinating spores of Ganoderma lucidum, Cancer Lett., 182, 155, 10.1016/S0304-3835(02)00080-0 Liu, 2010, Characterization and antioxidant activity of two low-molecular-weight polysaccharides purified from the fruiting bodies of Ganoderma lucidum, Int. J. Biol. Macromol., 46, 451, 10.1016/j.ijbiomac.2010.02.006 Liu, 2012, The polysaccharides from Ganoderma lucidum: Are they always inhibitors on human hepatocarcinoma cells?, Carbohydr. Polym., 90, 1210, 10.1016/j.carbpol.2012.06.043 Lombard, 1994, A new method for studying the binding and ingestion of zymosan particles by macrophages, J. Immunol. Methods, 174, 155, 10.1016/0022-1759(94)90018-3 Lowe, 2001, A (1→3)-β-D-linked heptasaccharide is the unit ligand for glucan pattern recognition receptors on human monocytes, Microbes Infect., 3, 789, 10.1016/S1286-4579(01)01436-8 Ma, 2013, Optimization for the extraction of polysaccharides from Ganoderma lucidum and their antioxidant and antiproliferative activities, J. Taiwan. Inst. Chem. Eng., 44, 886, 10.1016/j.jtice.2013.01.032 Mahendran, 2013, Antibacterial potential of microbial exopolysaccharide from Ganoderma lucidum and Lysinibacillus fusiformis, Int. J. Rec. Sci. Res., 4, 501 Miles, 1997 Miura, 2002, Isoflavone aglycon produced by culture of soybean extracts with Basidiomycetes and its anti-angiogenic activity, Biosci. Biotechnol. Biochem., 66, 2626, 10.1271/bbb.66.2626 Miyazaki, 1981, Studies on fungal polysaccharides. XXVII. Structural examination of a water-soluble, antitumor polysaccharide of Ganoderma lucidum, Chem. Pharm. Bull. (Tokyo), 29, 3611, 10.1248/cpb.29.3611 Mizuno, 1995, Reishi, Ganoderma lucidum and Ganoderma tsugae: bioactive substances and medicinal effects, Food Rev. Int., 11, 151, 10.1080/87559129509541025 Mizuno, 1995, Antitumor active substances from mushrooms, Food Rev. Int., 11, 23, 10.1080/87559129509541018 Mizuno, 1996, Antitumor activity and chemical modification of polysaccharides from Niohshimeji mushroom, Tricholoma giganteum, Biosci. Biotechnol. Biochem., 60, 30, 10.1271/bbb.60.30 Mizuno, 1999, The extraction and development of antitumor active polysaccharides from medicinal mushrooms in Japan, Int. J. Med. Mushr., 1, 9, 10.1615/IntJMedMushrooms.v1.i1.20 Mizushina, 1998, A mushroom fruiting body-inducing substance inhibits activities of replicative DNA polymerases, Biochem. Biophys. Res. Commun., 249, 12, 10.1006/bbrc.1998.9091 Moradali, 2007, Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi), Int. Immunopharmacol., 7, 701, 10.1016/j.intimp.2007.01.008 Nie, 2013, Current development of polysaccharides from Ganoderma: isolation, structure and bioactivities, Bioact. Carbohydrates Dietary Fibre, 1, 10, 10.1016/j.bcdf.2013.01.001 Ooi, 1999, A review of pharmacological activities of mushroom polysaccharides, Int. J. Med. Mushr., 1, 195, 10.1615/IntJMedMushrooms.v1.i3.10 Ooi, 2002, Induction of gene expression of immunomodulatory cytokines in the mouse by a polysaccharide from Ganoderma lucidum (Curt.: Fr.) P. Karst. (Aphyllophoromycetideae), Int. J. Med. Mushr., 4, 10.1615/IntJMedMushr.v4.i1.30 Pan, 2013, Optimization extraction of Ganoderma lucidum polysaccharides and its immunity and antioxidant activities, Int. J. Biol. Macromol., 55, 301, 10.1016/j.ijbiomac.2013.01.022 Patel, 2012, Recent developments in mushrooms as anti-cancer therapeutics: a review, 3 Biochem., 2, 1 Paterson, 2006, Ganoderma – A therapeutic fungal biofactory, Phytochem, 67, 1985, 10.1016/j.phytochem.2006.07.004 Peng, 2005, Structure and antitumor activities of the water-soluble polysaccharides from Ganoderma tsugae mycelium, Carbohydr. Polym., 59, 385, 10.1016/j.carbpol.2004.10.009 Pincemail, 1995, Free radicals and antioxidants in human diseases, 83 Popović, 2013, Mycotherapy of cancer: an update on cytotoxic and antitumor activities of mushrooms, bioactive principles and molecular mechanisms of their action, Curr. Top. Med. Chem., 13, 2791, 10.2174/15680266113136660198 Quereshi, 2010, Evaluation of antibacterial activity of different Ganoderma lucidum extracts, J. Sci. Res., 3, 9 Ramberg, 2010, Immunomodulatory dietary polysaccharides: a systematic review of the literature, Nutr. J., 9, 1, 10.1186/1475-2891-9-54 Roupas, 2012, The role of edible mushrooms in health: evaluation of the evidence, J. Funct. Food, 4, 687, 10.1016/j.jff.2012.05.003 Sazaki, 1971, Antitumor polysaccharides from some Polyporaceae, Ganoderma applanatum (Pers.) Pat and Phellinus linteus (Berk. Et Curt) Aoshima, Chem. Pharm. Bull., 19, 821, 10.1248/cpb.19.821 Sheena, 2003, Antibacterial activity of three macrofungi, Ganoderma lucidum, Navesporus floccosa and Phellinus rimosus occurring in South India, Pharmac. Biol., 41, 564, 10.1080/13880200390501226 Shi, 2010, Extraction, characterization of the polysaccharide extracts from Se-enriched G. lucidum (Se-GLP) and its inhibition against oxidative damage in ischemic reperfusion mice, Carbohydr. Polym., 80, 774, 10.1016/j.carbpol.2009.12.027 Shi, 2013, Antioxidant and immunoregulatory activity of Ganoderma lucidum polysaccharide, Carbohydr. Polym., 95, 200, 10.1016/j.carbpol.2013.02.081 Skalicka-Woźniak, 2012, Evaluation of polysaccharides content in fruit bodies and their antimicrobial activity of four Ganoderma lucidum (W Curt.: Fr.) P. Karst strains cultivated on different wood type substrates, Acta Soc. Bot. Pol., 81, 17, 10.5586/asbp.2012.001 Sliva, 2003, Ganoderma lucidum (Reishi) in cancer treatment, Integr. Cancer Ther., 2, 358, 10.1177/1534735403259066 Sone, 1985, Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidum, Agric. Biol. Chem., 49, 2641 Soniamol, 2011, Antitumor and anti-inflammatory activities of polysaccharides isolated from Ganoderma lucidum, Acta Pharm., 61, 335, 10.2478/v10007-011-0030-6 Staub, 1999, Removal of protein. Sevag method, Methods Carbohydr. Chem., 5, 5 Sullivan, 2006, Medicinal mushrooms and cancer therapy. Translating a traditional practice into Western medicine, Perspect. Biol. Med., 49, 159, 10.1353/pbm.2006.0034 Sun, 2004, Novel antioxidant peptides from fermented mushroom Ganoderma lucidum, J. Agric. Food Chem., 52, 6646, 10.1021/jf0495136 Tu, T.-R., Li, C.-F., Su, S.-H., Wong, C.-H., Fan, E., 2011. Compositions and methods for treating psoriasis by Ganoderma lucidum (Reishi) polysaccharides. US7947283 B2. Ukai, 1982, Structure of an alkali-soluble polysaccharide from the fruit body of Ganoderma japonicum Lloyd, Carbohydr. Res., 105, 237, 10.1016/S0008-6215(00)84971-X Varki, 1999 Wang, 2011, Polysaccharides purified from the submerged culture of Ganoderma formosanum stimulate macrophage activation and protect mice against Listeria monocytogenes infection, Biotechnol. Lett., 33, 2271, 10.1007/s10529-011-0697-2 Wang, 1993, Antitumor active polysaccharides from the Chinese mushroom Songshan lingzhi, the fruiting body of Ganoderma tsugae, Biosci. Biotechnol. Biochem., 57, 894, 10.1271/bbb.57.894 Wang, 2011, Structure and chain conformation of water-soluble heteropolysaccharides from Ganoderma lucidum, Carbohydr. Polym., 86, 844, 10.1016/j.carbpol.2011.05.031 Wang, 2009, Structure and chain conformation of five water soluble derivatives of a β-D-glucan isolated from Ganoderma lucidum, Carbohydr. Res., 344, 105, 10.1016/j.carres.2008.09.024 Wang, 1997, The antitumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophagues and T lymphocytes, Int. J. Cancer, 70, 699, 10.1002/(SICI)1097-0215(19970317)70:6<699::AID-IJC12>3.0.CO;2-5 Wang, 2002, Studies on the immuno- modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: Functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities, Bioorg. Med. Chem., 10, 1057, 10.1016/S0968-0896(01)00377-7 Wang, Y.-Y., Koo, K.-H., Chen, S.-T., Lin, C.-C., Wong, C.-H., Lin, C.-H., Chen, H.-S., Tsai, Y.-F., 2006. Immuno-modulating antitumor activities of Ganoderma lucidum (Reishi) polysaccharides. US7135183 B1. Wasser, 1999, Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective, Crit. Rev. Immunol., 19, 65 Wasser, 2002, Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides, Appl. Microbiol. Biotechnol., 60, 258, 10.1007/s00253-002-1076-7 Wasser, 2011, Current findings, future trends, and unsolved problems in studies of medicinal mushrooms, Appl. Microbiol. Biotechnol., 89, 1323, 10.1007/s00253-010-3067-4 Wong, C.-H., Hsu, H.-Y., Hua, K.-F., Lin, C.-H., Hsu, J., Chen, S.-T., Lin, K.-I., Yang, W.-B., Yu, J., Yu, A., 2006. Methods and compositions associated with administration of an extract of Ganoderma lucidum. WO2006044616 A2. XiaoPing, 2009, Free radical scavenging of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes and immunity activities in cervical carcinoma rats, Carbohydr. Polym., 77, 389, 10.1016/j.carbpol.2009.01.009 Xu, 2007, Microwave-assistant extraction of Ganoderma lucidum polysaccharide, Pharm. Clin. Res., 15, 297 Yan, 1999, Beta-glucan, a “specific” biologic response modifier that uses antibodies to target tumors for cytotoxic recognition by leukocyte complement receptor type 3 (CD11b/CD18), J. Immunol., 163, 3045, 10.4049/jimmunol.163.6.3045 Yang, 2010, HPLC analysis of Ganoderma lucidum polysaccharides and its effect on antioxidant enzymes activity and Bax, Bcl-2 expression, Int. J. Biol. Macromol., 46, 167, 10.1016/j.ijbiomac.2009.11.002 Ye, 2009, Structural characterisation of a heteropolysaccharide by NMR spectra, Food Chem., 112, 962, 10.1016/j.foodchem.2008.07.017 Yuen, 2005, Anticancer effects of Ganoderma lucidum: a review of scientific evidence, Nutr. Cancer, 53, 11, 10.1207/s15327914nc5301_2 YouGuo, 2009, Modulatory effect of Ganoderma lucidum polysaccharides on serum antioxidant enzymes activities in ovarian cancer rats, Carbohydr. Polym., 78, 258, 10.1016/j.carbpol.2009.03.030 Yu-Hong, 2002, Protective effects of Ganoderma lucidum polysaccharides peptide on injury of macrophages induced by reactive oxygen species, Acta Pharmacol. Sin., 23, 787 Zhang, 1994, Antitumor active protein-containing glycans from the Chinese mushroom Songshan Lingzhi, Ganoderma tsugae mycelium, Biosci. Biotech. Biochem., 58, 1202, 10.1271/bbb.58.1202 Zhang, 1999, The antitumor activity of Ganoderma lucidum (Curt.:Fr.) P. Karst. (Ling Zhi) (Aphyllophoromycetideae) polysaccharides is related to tumor necrosis factor-α and interferon-γ, Int. J. Med. Mushr., 5, 207, 10.1615/IntJMedMushrooms.v1.i3.20 Zhang, 2002, Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum, Life Sci., 71, 623, 10.1016/S0024-3205(02)01690-9 Zhang, 2003, In vitro and in vivo protective effect of Ganoderma lucidum polysaccharides on alloxan-induced pancreatic islets damage, Life Sci., 73, 2307, 10.1016/S0024-3205(03)00594-0 Zhang, 2006, Purification and characterization of water soluble polysaccharide GLPS 3 from sporderm-unbroken germinting spors of Ganoderma lucidum, Chin. Pharm. J., 41, 230 Zhang, 2007, Antitumor polysaccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity, Trend Food Sci. Technol., 18, 4, 10.1016/j.tifs.2006.07.013 Zhang, 2010, GLIS, a bioactive proteoglycan fraction from Ganoderma lucidum, displays anti-tumour activity by increasing both humoral and cellular immune response, Life Sci., 87, 19, 10.1016/j.lfs.2010.09.026 Zhang, 2012, Antitumor activity of sulfated extracellular polysaccharides of Ganoderma lucidum from the submerged fermentation broth, Carbohydr. Polym., 87, 1539, 10.1016/j.carbpol.2011.09.051 Zhang, 2013, Immunomodulatory effect of Ganoderma atrum polysaccharide on CT26 tumor-bearing mice, Food Chem., 136, 1213, 10.1016/j.foodchem.2012.08.090 Zhao, 2004, Polysaccharide extract isolated from Ganoderma lucidum protects rat cerebral cortical neurons from hypoxia/reoxygenation injury, J. Pharmacol. Sci., 95, 294, 10.1254/jphs.SC0040011 Zhao, 2005, Chemical structure of water soluble polysaccharide from spores of Ganoderma lucidum, Chin. Pharm. J., 41, 902 Zhao, 2010, Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum, Carbohydr. Polym., 80, 783, 10.1016/j.carbpol.2009.12.029 Zhao, 2012, Antioxidant activities of Ganoderma lucidum polysaccharides and their role on DNA damage in mice induced by cobalt-60 gamma-irradiation, Food Chem. Toxicol., 50, 303, 10.1016/j.fct.2011.10.071 Zhonghui, 2013, Ganoderma lucidum supplemetation attenuates exercise-induced oxidative stress in skeletal muscle of mice, Saudi J. Biol. Sci. Zhuang, 1993, Antitumor protein-containing polysaccharides from a Chinese mushroom Fengweigu or Houbitake, Pleurotus sajor-caju (Fr.) Sing, Biosci. Biotechnol. Biochem., 57, 901, 10.1271/bbb.57.901 Zhuang, 1994, Chemical modification and antitumor activity of polysaccharides from the mycelium of liquid-cultured Grifola frondosa, Nippon Shokuhin Kogyo Gakkaishi, 41, 733, 10.3136/nskkk1962.41.733 Zong, 2012, Anticancer polysaccharides from natural resources: a review of recent research, Carbohydr. Polym., 90, 1395, 10.1016/j.carbpol.2012.07.026