A survey of the mouse hindbrain in the fed and fasted states using single-nucleus RNA sequencing

Price, 2007, The area postrema: a brain monitor and integrator of systemic autonomic state, The Neuroscientist, 14, 182, 10.1177/1073858407311100 Gasparini, 2020, Central afferents to the nucleus of the solitary tract in rats and mice, Journal of Comparative Neurology, 528, 2708, 10.1002/cne.24927 Grill, 2012, Hindbrain neurons as an essential hub in the neuroanatomically distributed control of energy balance, Cell Metabolism, 16, 296, 10.1016/j.cmet.2012.06.015 Pi-Sunyer, 2015, A randomized, controlled trial of 3.0 mg of liraglutide in weight management, New England Journal of Medicine, 373, 11, 10.1056/NEJMoa1411892 Wilding, 2021, Once-weekly semaglutide in adults with overweight or obesity, New England Journal of Medicine, 10.1056/NEJMoa2032183 Sisley, 2014, Neuronal GLP1R mediates liraglutide's anorectic but not glucose-lowering effect, Journal of Clinical Investigation, 124, 2456, 10.1172/JCI72434 Fortin, 2020, GABA neurons in the nucleus tractus solitarius express GLP-1 receptors and mediate anorectic effects of liraglutide in rats, Science Translational Medicine, 12, 10.1126/scitranslmed.aay8071 Secher, 2014, The arcuate nucleus mediates GLP-1 receptor agonist liraglutide-dependent weight loss, Journal of Clinical Investigation, 124, 4473, 10.1172/JCI75276 Liberini, 2016, The satiating hormone amylin enhances neurogenesis in the area postrema of adult rats, Molecular Metabolism, 5, 834, 10.1016/j.molmet.2016.06.015 Patel, 2019, GDF15 provides an endocrine signal of nutritional stress in mice and humans, Cell Metabolism, 29, 707, 10.1016/j.cmet.2018.12.016 Liberini, 2019, Combined Amylin/GLP-1 pharmacotherapy to promote and sustain long-lasting weight loss, Scientific Reports, 9, 8447, 10.1038/s41598-019-44591-8 Adriaenssens, 2019, Glucose-Dependent insulinotropic polypeptide receptor-expressing cells in the hypothalamus regulate food intake, Cell Metabolism, 30, 987, 10.1016/j.cmet.2019.07.013 Mathiesen, 2019, The effects of dual GLP-1/GIP receptor agonism on glucagon secretion-A review, International Journal of Molecular Sciences, 20, 4092, 10.3390/ijms20174092 Campbell, 2017, A molecular census of arcuate hypothalamus and median eminence cell types, Nature Neuroscience, 20, 484, 10.1038/nn.4495 Chen, 2017, Single-cell RNA-seq reveals hypothalamic cell diversity, Cell Reports, 18, 3227, 10.1016/j.celrep.2017.03.004 Lam, 2017, Heterogeneity of hypothalamic pro-opiomelanocortin-expressing neurons revealed by single-cell RNA sequencing, Molecular Metabolism, 6, 383, 10.1016/j.molmet.2017.02.007 Romanov, 2017, Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes, Nature Neuroscience, 20, 176, 10.1038/nn.4462 Zhang, 2020, Area postrema cell types that mediate nausea-associated behaviors, Neuron Butler, 2018, Integrating single-cell transcriptomic data across different conditions, technologies, and species, Nature Biotechnology, 36, 411, 10.1038/nbt.4096 Stuart, 2019, Comprehensive integration of single-cell data, Cell, 177, 1888, 10.1016/j.cell.2019.05.031 McCarthy, 2012, Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation, Nucleic Acids Research, 40, 4288, 10.1093/nar/gks042 Mei, 2014, Neuregulin-ERBB signaling in the nervous system and neuropsychiatric diseases, Neuron, 83, 27, 10.1016/j.neuron.2014.06.007 Mironova, 2016, PI(3,5)P2 biosynthesis regulates oligodendrocyte differentiation by intrinsic and extrinsic mechanisms, eLife, 5, 10.7554/eLife.13023 Vemuri, 1996, Oligodendrocytes and their precursors require phosphatidylinositol 3-kinase signaling for survival, Development, 122, 2529, 10.1242/dev.122.8.2529 Morita, 2016, Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase, Scientific Reports, 6, 20995, 10.1038/srep20995 Boyle, 2018, Amylin – its role in the homeostatic and hedonic control of eating and recent developments of amylin analogs to treat obesity, Molecular Metabolism, 8, 203, 10.1016/j.molmet.2017.11.009 Yang, 2017, GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand, Nature Medicine, 23, 1158, 10.1038/nm.4394 Emmerson, 2017, The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL, Nature Medicine, 23, 1215, 10.1038/nm.4393 Hsu, 2017, Non-homeostatic body weight regulation through a brainstem-restricted receptor for GDF15, Nature, 550, 255, 10.1038/nature24042 Mullican, 2017, GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates, Nature Medicine, 23, 1150, 10.1038/nm.4392 Harno, 2018, POMC: the physiological power of hormone processing, Physiological Reviews, 98, 2381, 10.1152/physrev.00024.2017 Krishnaswami, 2016, Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons, Nature Protocols, 11, 499, 10.1038/nprot.2016.015 Wu, 2019, Advantages of single-nucleus over single-cell RNA sequencing of adult kidney: rare cell types and novel cell states revealed in fibrosis, Journal of the American Society of Nephrology, 30, 23, 10.1681/ASN.2018090912 Bakken, 2018, Single-nucleus and single-cell transcriptomes compared in matched cortical cell types, PloS One, 13, 10.1371/journal.pone.0209648 Deng, 2020, Single-nucleus RNA sequencing of the hypothalamic arcuate nucleus of C57bl/6J mice after prolonged diet-induced obesity, Hypertension, 76, 589, 10.1161/HYPERTENSIONAHA.120.15137 Weng, 2017, Transcription factor 7 like 2 promotes oligodendrocyte differentiation and remyelination, Molecular Medicine Reports, 16, 1864, 10.3892/mmr.2017.6843 Kohnke, 2019, Nutritional signals rapidly activate oligodendrocyte differentiation in the adult hypothalamic median eminence, bioRxiv, 751198 Gabery, 2020, Semaglutide lowers body weight in rodents via distributed neural pathways, JCI insight, 5, 10.1172/jci.insight.133429 Willard, 2020, Tirzepatide is an imbalanced and biased dual GIP and GLP-1 receptor agonist, JCI insight, 5, 10.1172/jci.insight.140532 Zhang, 2021, The glucose-dependent insulinotropic polypeptide (GIP) regulates body weight and food intake via CNS-GIPR signaling, Cell Metabolism Killion, 2018, Anti-obesity effects of GIPR antagonists alone and in combination with GLP-1R agonists in preclinical models, Science Translational Medicine, 10, 10.1126/scitranslmed.aat3392 Larsen, 2021, The dual amylin and calcitonin receptor agonist KBP-089 and the GLP-1 receptor agonist liraglutide act complimentarily on body weight reduction and metabolic profile, BMC Endocrine Disorders, 21, 10, 10.1186/s12902-020-00678-2 Cheng, 2020, Calcitonin receptor neurons in the mouse nucleus tractus solitarius control energy balance via the non-aversive suppression of feeding, Cell Metabolism, 31, 301, 10.1016/j.cmet.2019.12.012 Georgescu, 2020, Neurochemical characterization of brainstem pro-opiomelanocortin cells, Endocrinology, 161, 10.1210/endocr/bqaa032 Ellacott, 2006, Characterization of leptin-responsive neurons in the caudal brainstem, Endocrinology, 147, 3190, 10.1210/en.2005-0877 Huo, 2006, Divergent regulation of proopiomelanocortin neurons by leptin in the nucleus of the solitary tract and in the arcuate hypothalamic nucleus, Diabetes, 55, 567, 10.2337/diabetes.55.03.06.db05-1143 Rau, 2018, Various transgenic mouse lines to study proopiomelanocortin cells in the brain stem label disparate populations of GABAergic and glutamatergic neurons, American Journal of Physiology. Regulatory, Integrative and Comparative Physiology, 315, R144, 10.1152/ajpregu.00047.2018