808-nm laser therapy with a flat-top handpiece photobiomodulates mitochondria activities of Paramecium primaurelia (Protozoa)

Lasers in Medical Science - Tập 31 - Trang 741-747 - 2016
Andrea Amaroli1, Silvia Ravera2, Steven Parker3, Isabella Panfoli2, Alberico Benedicenti3, Stefano Benedicenti3
1Department of Earth, Environmental and Life Sciences, Protistology Laboratory, University of Genoa, Genoa, Italy
2Department of Pharmacy, Biochemistry Laboratory, University of Genoa, Genoa, Italy
3Department of Surgical Sciences and Integrated Diagnostic, University of Genoa, Genoa, Italy

Tóm tắt

Photobiomodulation is proposed as a non-linear process, and only low-level laser therapy (LLLT) is assumed to stimulate exposed cells, whereas high powered laser and fluences can cause negative effects, exhausting the cell’s energy reserve as a consequence of excessive photon-based stimulation. In our work, we investigated and compared the effects of 808-nm diode laser (CW) with a new flat-top handpiece. To this purpose, we tested the photobiomodulation effects of 1 and 3 J/cm2 fluence, both generated by 100 mW or 1 W of laser power and of 64 J/cm2 of fluence generated by 100 mW, 1 W, 1.5 W or 2 W, as expressed through oxygen consumption and ATP synthesis of Paramecium. Data collected indicates the incremental consumption of oxygen through irradiation with 3 J/cm2–100 mW or 64 J/cm2–1 W correlates with an increase in Paramecium ATP synthesis. The Paramecium respiration was inhibited by fluences 64 J/cm2–100 mW or 64 J/cm2–2 W and was followed by a decrease in the endogenous ATP concentration. The 1 J/cm2–100 mW or 1 W and 3 J/cm2–1 W did not affect mitochondrial activity. The results show that the fluence of 64 J/cm2–1 W more than the 3 J/cm2–100 mW causes greater efficiency in Paramecium mitochondria respiratory chain activity. Our results suggest that thanks to flat-top handpiece we used, high fluences by high-powered laser have to be reconsidered as an effective and non-invasive therapy. Possible associated benefits of deeper tissue penetration would increase treatment effectiveness and reduced irradiation time.

Tài liệu tham khảo

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