5′-YRNA fragments derived by processing of transcripts from specific YRNA genes and pseudogenes are abundant in human serum and plasma

Physiological Genomics - Tập 45 Số 21 - Trang 990-998 - 2013
Joseph M. Dhahbi1, Stephen R. Spindler2, Hani Atamna3, Dario Boffelli4, Patricia L. Mote2, David I. K. Martin4
1Department of Biochemistry, University of California at Riverside, Riverside, California;
21Department of Biochemistry, University of California at Riverside, Riverside, California;
33Department of Basic Sciences, Neuroscience, The Commonwealth Medical College, Scranton, Pennsylvania
42Center for Genetics, Childrens Hospital Oakland Research Institute, Oakland, California;

Tóm tắt

Small noncoding RNAs carry out a variety of functions in eukaryotic cells, and in multiple species they can travel between cells, thus serving as signaling molecules. In mammals multiple small RNAs have been found to circulate in the blood, although in most cases the targets of these RNAs, and even their functions, are not well understood. YRNAs are small (84–112 nt) RNAs with poorly characterized functions, best known because they make up part of the Ro ribonucleoprotein autoantigens in connective tissue diseases. In surveying small RNAs present in the serum of healthy adult humans, we have found YRNA fragments of lengths 27 nt and 30–33 nt, derived from the 5′-ends of specific YRNAs and generated by cleavage within a predicted internal loop. Many of the YRNAs from which these fragments are derived were previously annotated only as pseudogenes, or predicted informatically. These 5′-YRNA fragments make up a large proportion of all small RNAs (including miRNAs) present in human serum. They are also present in plasma, are not present in exosomes or microvesicles, and circulate as part of a complex with a mass between 100 and 300 kDa. Mouse serum contains far fewer 5′-YRNA fragments, possibly reflecting the much greater copy number of YRNA genes and pseudogenes in humans. The function of the 5′-YRNA fragments is at present unknown, but the processing and secretion of specific YRNAs to produce 5′-end fragments that circulate in stable complexes are consistent with a signaling function.

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Tài liệu tham khảo

10.3892/ijo.2012.1647

10.1073/pnas.1019055108

Bouffard P, 1996, J Rheumatol, 23, 1838

10.1093/nar/gkn787

10.1007/s00109-004-0529-0

10.1093/bioinformatics/btp250

10.1186/1471-2164-14-298

10.18632/aging.100540

10.1074/jbc.M109.077560

10.1038/nrg3074

10.1016/j.mrfmmm.2011.03.004

10.1016/j.febslet.2008.12.043

10.1186/1471-2334-13-285

10.1093/nar/gkg599

10.1016/j.molcel.2011.06.022

10.1101/cshperspect.a003772

10.1126/science.1138341

10.1186/gb-2009-10-3-r25

10.1126/science.6164096

10.1038/onc.2011.304

10.1016/j.canlet.2012.11.058

10.1093/nar/gkq376

10.1016/j.febslet.2012.03.026

10.1093/nar/gks658

10.1002/wrna.113

10.1038/nprot.2008.67

10.1093/nar/gki504

10.1093/molbev/msm084

10.1093/bioinformatics/btq033

10.4049/jimmunol.1202849

10.1016/j.biochi.2011.07.032

10.1074/jbc.274.35.24799

10.1074/jbc.M112.371799

10.1038/leu.2008.286

10.1002/wrna.85

10.1093/nar/28.2.610

10.1016/j.tig.2011.06.001

10.4161/rna.21083

10.1093/nar/gkr254

10.1093/nar/22.13.2498

10.1038/ncb2210

10.1093/nar/gkq601

10.1002/wsbm.148

10.1016/0092-8674(83)90059-4

10.1002/med.20215

10.1126/scisignal.2000610

Zhang C, 2009, Curr Opin Mol Therapeut, 11, 641

10.1104/pp.108.134767

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Physiological Genomics

Tập 45 Số 21

990-998

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