4‐Hydroxynonenal Induces Oxidative Stress and Death of Cultured Spinal Cord Neurons

Journal of Neurochemistry - Tập 74 Số 6 - Trang 2278-2287 - 2000
Andrzej Małecki1,2, Angelika Bátorová2, Mark P. Mattson3, Bernhard Hennig4, Michał Toborek2
1Department of Pharmacology, Silesian Medical University, Katowice, Poland
2Department of Surgery, University of Kentucky, Lexington, Kentucky, U.S.A.
3Sanders—Brown Research Center on Aging, University of Kentucky, Lexington, Kentucky, U.S.A.
4Department of Nutrition and Food Science, University of Kentucky, Lexington, Kentucky, U.S.A.

Tóm tắt

Abstract: Primary spinal cord trauma can trigger a cascade of secondary processes leading to delayed and amplified injury to spinal cord neurons. Release of fatty acids, in particular arachidonic acid, from cell membranes is believed to contribute significantly to these events. Mechanisms of fatty acid‐induced injury to spinal cord neurons may include lipid peroxidation. One of the major biologically active products of arachidonic acid peroxidation is 4‐hydroxynonenal (HNE). The levels of HNE‐protein conjugates in cultured spinal cord neurons increased in a dose‐dependent manner after a 24‐h exposure to arachidonic acid. To study cellular effects of HNE, spinal cord neurons were treated with different doses of HNE, and cellular oxidative stress, intracellular calcium, and cell viability were determined. A 3‐h exposure to 10 μM HNE caused ∼80% increase in oxidative stress and 30% elevation of intracellular calcium. Exposure of spinal cord neurons to HNE caused a dramatic loss of cellular viability, indicated by a dose‐dependent decrease in MTS [3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium, inner salt] conversion. The cytotoxic effect of HNE was diminished by pretreating neurons with ebselen or N‐acetylcysteine. These data support the hypothesis that formation of HNE may be responsible, at least in part, for the cytotoxic effects of membrane‐released arachidonic acid to spinal cord neurons.

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Journal of Neurochemistry

Tập 74 Số 6

2278-2287

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