3D Bioprinting of Self‐Standing Silk‐Based Bioink

Advanced healthcare materials - Tập 7 Số 6 - 2018
Zhaozhu Zheng1, Jianbing Wu1, Meng Liu2, Heng Wang1, William P. Meehan3, Marí­a José Rodrí­guez3, Gang Li1, Xiaoqin Wang1, David L. Kaplan3
1National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, Suzhou 215123, People's Republic of China
2The Cyrus Tang Hematology Center, Soochow University, Suzhou, 215123 People's Republic of China
3Department of Biomedical Engineering, Tufts University, 4, Colby street, Medford, MA 02155 (USA)

Tóm tắt

AbstractSilk/polyethylene glycol (PEG) hydrogels are studied as self‐standing bioinks for 3D printing for tissue engineering. The two components of the bioink, silk fibroin protein (silk) and PEG, are both Food and Drug Administration approved materials in drug and medical device products. Mixing PEG with silk induces silk β‐sheet structure formation and thus gelation and water insolubility due to physical crosslinking. A variety of constructs with high resolution, high shape fidelity, and homogeneous gel matrices are printed. When human bone marrow mesenchymal stem cells are premixed with the silk solution prior to printing and the constructs are cultured in this medium, the cell‐loaded constructs maintain their shape over at least 12 weeks. Interestingly, the cells grow faster in the higher silk concentration (10%, w/v) gel than in lower ones (7.5 and 5%, w/v), likely due to the difference in material stiffness and the amount of residual PEG remaining in the gel related to material hydrophobicity. Subcutaneous implantation of 7.5% (w/v) bioink gels with and without printed fibroblast cells in mice reveals that the cells survive and proliferate in the gel matrix for at least 6 week postimplantation. The results suggest that these silk/PEG bioink gels may provide suitable scaffold environments for cell printing and function.

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Advanced healthcare materials

Tập 7 Số 6

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