25-bp long border sequence of-TDNA is sufficient for cleavage byvir D endonuclease ofAgrobacterium
Tóm tắt
Agrobacterium tumefaciens-induced tumor formation in plant cells involves transfer of a defined segment of DNA (T-DNA) from the bacterium into plant cells. This process is known to involvevir genes, 25-bp-long T-DNA border sequences and additional sequences adjacent to border sequences called overdrive sequences. It is also known that thevir D gene-encoded endonuclease causes cleavage at the border sequences. In this report we show that a synthetic 25-bp border sequence is sufficient for cleavage and that overdrive sequences are not necessary for the cleavage catalysed byvirD endonuclease.
Tài liệu tham khảo
Albright L., Yanofsky M. F., Leroux B., Ma D. and Nester E. W. 1987 Processing of the T-DNA ofAgrobacterium tumefaciens generates a single-stranded linear intermediate.J. Bacteriol. 169: 1046–1055
An G. 1985 High efficiency transformation of cultured tobacco cells.Plant Physiol. 79: 568–570
Barry G. F., Rogers S. G., Fraley R. T. and Brand L. 1984 Identification of a cloned cytokinin biosynthetic gene.Proc. Natl. Acad. Sci. USA 81: 4776–4780
Binns A. N. and Thomshow M. F. 1988 Cell biology ofAgrobacterium infection and transformation of plants.Annu. Rev. Microhiol. 42: 575–606
Birnboim H. C. and Doly J. 1979 A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Res. 7: 1513–1523
Chilton M. D., Currier T. G., Farrond S. K., Bendish A. J., Gordon M. P. and Nester E. W. 1974Agrobacterium DNA and PS8 bacteriophage DNA not detected in crown gall tumors.Proc. Natl. Acad. Sci. USA 71: 3672–3676
DeCleene M. and Deley J. 1976 The host range of crown gall.Bot. Rev. 42: 389–466
Hille J., van Kan J. and Schilperoort R. A. 1984 Trans-acting virulence functions of the octopine Ti plasmid fromAgrobacterium tumefaciens.J. Bacteriol. 158: 754–756
Holsters M., Villarroel R., Gielen J., Seurinck J., De Greeve H., Van Montagu M. and Schell J. 1983 An analysis of the boundaries of the Octopine Ti-DNA in tumors induced byAgrobacterium tumefaciens.Mol. Gen. Genet. 190: 35–41
Klee H. J., Horsch R. B. and Rogers S. 1987 Agrobacterium mediated plant transformation and its further application to biology.Annu. Rev. Plant Physiol. 38: 467–486
Miller J. H. 1972Experiments in molecular genetics (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory) p. 21
Murashige T. and Skoog F. 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures.Physiol. Plant. 15: 473–497
Nester E. W., Gordon M. P., Amasino R. M. and Yanofsky M. F. 1984 Crown gall: A molecular and physiological analysis.Annu. Rev. Plant Physiol. 35: 387–413
Peralta E. G., Hellmiss R. and Rean W. 1986Overdrive, a T-DNA transmission enhancer on theA. tumefaciens tumor inducing plasmid.EMBO J. 5: 1137–1142
Peralta E. G. and Ream W. 1985 T-DNA border sequences required for crown gall tumorigenesis.Proc. Natl. Acad. Sci. USA 82: 5112–5116
Rubin R. A. 1986 Genetic studies on the role of Octopine T-DNA border region in crown gall tumor formation.Mol. Gen. Genet. 202: 312–320
Schröder G., Waffenschmidt S., Weiler E. W. and Schroder J. 1983 The region of Ti plasmid codes for an enzyme synthesizing indoleacetic acid.EMBO J. 2: 403–409
Stachel S. E. and Nester E. W. 1986 The genetic and transcriptional organization of thevir region of the A6 Ti plasmid ofAgrobacterium tumefaciens.EMBO J. 5: 1445–1454
Stachel S. E., Nester E. W. and Zambryski P. 1986a A plant cell factor inducesAgrobacterium tumefaciens vir gene expression.Proc. Natl. Acad. Sci. USA 83: 379–383
Stachel S. E., Timmerman B. and Zambryski P. 1986b Generation of single-stranded T-DNA molecule during the initial stages of T-DNA transfer fromAgrobacterium tumefaciens of plant cells.Nature (London) 322: 706–712
Stachel S. E. and Zambryski P. 1986vir A andvirG control the plant induced activation of the T-DNA transfer process ofAgrobacterium tumefaciens.Cell 46: 325–333
Thomshow L., Reeves S. and Thomshow M. F. 1984 Crown gall oncogenesis: Evidence that a T-DNA gene from theAgrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyses synthesis of indoleacetic acid.Proc. Natl. Acad. Sci. USA 81: 5071–5075
Toro N., Datta A., Yanofsky M. F. and Nester E. 1988 Role of the overdrive sequence in T-DNA border cleavage inAgrobacterium.Proc. Natl. Acad. Sci. USA 85: 8558–8562
Wang K., Genetello C., Van Montagu M. and Zambryski P. C. 1987a Sequence context of the T-DNA border repeat element determines its relative activity during T-DNA transfer to plant cells.Mol. Gen. Genet. 210: 338–346
Wang K., Herrera-Estrella L., Van Montagu M. and Zambryski P. 1984 Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines DNA transfer fromAgrobacterium to the plant genome.Cell 38: 455–462
Wang K., Stachel S. E., Timmerman, B., Van Montagu M. and Zambryski P. C. 1987b Site-specific nick in the T-DNA border sequence as a result ofAgrobacterhim vir gene expression.Science 235: 587–591
Yanofsky M. F., Porter S., Young C., Albright L., Gordon M. P. and Nester E. W. 1986 ThevirD operon ofAgrobacterium tumefaciens encodes a site-specific endonuclease.Cell 47: 471–477
Zambryski P., Tenpe J. and Schell J. 1989 Transfer and function of T-DNA genes fromAgrobacteriumTi-and Riplasmids in plants.Cell 56: 193–201