1H, 13C, 15N resonance assignments of the extracellular loop 1 domain (ECL1) of Streptococcus pneumoniae D39 FtsX, an essential cell division protein

Yue Fu1, Kevin E. Bruce2, Britta E. Rued2, Malcolm E. Winkler2, David P. Giedroc1
1Department of Chemistry, Indiana University, Bloomington, USA
2Department of Biology, Indiana University, Bloomington, USA

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Bartual SG, Straume D, Stamsas GA, Munoz IG, Alfonso C, Martinez-Ripoll M, Havarstein LS, Hermoso JA (2014) Structural basis of PcsB-mediated cell separation in Streptococcus pneumoniae. Nat Commun 5:3842

Bax A, Clore GM, Gronenborn AM (1990) 1H-1H correlation via isotropic mixing of 13C magnetization, a new three-dimensional approach for assigning 1H and 13C spectra of 13C-enriched proteins. J Magn Reson 88(2):425–431

Delaglio F, Grzesiek S, Vuister G, Zhu G, Pfeifer J, Bax A (1995) NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J Biomol NMR 6(3):277–293

Grzesiek S, Anglister J, Bax A (1993) Correlation of backbone amide and aliphatic side-chain resonances in 13C/15N-enriched proteins by isotropic mixing of 13C magnetization. J Magn Reson 101(1):114–119

Mavrici D, Marakalala MJ, Holton JM, Prigozhin DM, Gee CL, Zhang YJJ, Rubin EJ, Alber T (2014) Mycobacterium tuberculosis FtsX extracellular domain activates the peptidoglycan hydrolase, RipC. Proc Natl Acad Sci USA 111(22):8037–8042

Montelione GT, Lyons BA, Emerson SD, Tashiro M (1992) An efficient triple resonance experiment using carbon-13 isotropic mixing for determining sequence-specific resonance assignments of isotopically-enriched proteins. J Am Chem Soc 114(27):10974–10975

Sham LT, Barendt SM, Kopecky KE, Winkler ME (2011) Essential PcsB putative peptidoglycan hydrolase interacts with the essential FtsX(Spn) cell division protein in Streptococcus pneumoniae D39. Proc Natl Acad Sci USA 108(45):E1061–E1069

Sham LT, Jensen KR, Bruce KE, Winkler ME (2013) Involvement of FtsE ATPase and FtsX extracellular loops 1 and 2 in FtsEX-PcsB complex function in cell division of Streptococcus pneumoniae D39. MBio 4(4):e00431-13. doi: 10.1128/mBio.00431-13

Sheffield P, Garrard S, Derewenda Z (1999) Overcoming expression and purification problems of RhoGDI using a family of “parallel” expression vectors. Protein Expr Purific 15(1):34–39

Shen Y, Delaglio F, Cornilescu G, Bax A (2009) TALOS plus : a hybrid method for predicting protein backbone torsion angles from NMR chemical shifts. J Biomol NMR 44(4):213–223