16SrII phytoplasma associated with date palm and Mexican fan palm in Saudi Arabia

Springer Science and Business Media LLC - 2018
Ayman F. Omar1,2, Abdullah Alsohim1, Medhat R. Rehan3,1, Khaled A. Al-Jamhan1, Edel Pérez-López4
1Department of Crop Production and Protection, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia
2Department of plant pathology, Plant Pathology and Biotechnology Lab., Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt
3Department of Genetics, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, Egypt
4Department of Biology, University of Saskatchewan, Saskatoon, Canada

Tóm tắt

Date palm and Mexican fan palm trees showing symptoms previously associated with phytoplasmas were observed in the Al-Qassim region, Saudi Arabia in 2017. DNA amplification, sequencing, and phylogenetic analysis revealed the presence of a ‘Candidatus Phytoplasma australasia’- related strain in eighteen of the eighty-three symptomatic plants collected that were positive using 16S-based assays. This study confirmed the presence of phytoplasma affecting date palms in Saudi Arabia and it is the first report of several date palm cultivars associated with ‘Ca. P. australasia’-related strains. This is also the first report worldwide of Mexican fan palm trees affected by 16SrII phytoplasma strains and the first report of this plant host affected by phytoplasmas in Saudi Arabia. The implications of these findings are vital to implement management strategies and avoid economic losses in Saudi Arabia and the Middle East, which are the main producers of dates in the world.

Từ khóa


Tài liệu tham khảo

Aleid SM, Al-Khayri JM, Al-Bahrany AM (2015) Date palm status and perspective in Saudi Arabia. In: Al-Khayri JM, Jain SM, Johnson DV (eds) Date palm genetic resources and utilization. Asia and Europe, vol 2. Springer, Dordrecht, pp 49–95

Alhudaib K, Arocha Y, Wilson M, Jones P (2007) “Al-Wijam”, a new phytoplasma disease of date palm in Saudi Arabia. B Insectol 60:285–286

Alhudaib K, Rezk A, Alsalah M (2014) Phytoplasma disease in date palm in Saudi Arabia. Proceedings of the Fifth International Date Palm conference pp. 313–320

AlKhazindar M (2014) Detection and molecular identification of Aster yellows Phytoplasma in date palm in Egypt. J Phytopathol 162:621–625

Bertaccini A (2007) Phytoplasmas: diversity, taxonomy, and epidemiology. Front Life Sci 12:673–689

Bonfield JK, Whitwham A (2010) Gap5 - editing the billion fragment sequence assembly. Bioinformatics 26:1699–1703

Cronjé P, Dabek AJ, Jones P, Tymon AM (2000) First report of a phytoplasma associated with a disease of date palms in North Africa. New Dis Rep 1:4

Deng SJ, Hiruki C (1991) Genetic relatedness between two non-culturable mycoplasma-like organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology 81:1475–1479

Harrison NA, Helmick EE, Elliott ML (2008) Lethal yellowing-type diseases of palms associated with phytoplasmas newly identified in Florida, USA. Ann Appl Biol 153:85–94. https://doi.org/10.1111/j.1744-7348.2008.00240.x

Jarzembowski P, Komorowska B, Ptaszek M, Faltyn A, Proćków J (2018) First report of ‘Candidatus Phytoplasma asteris’ associated with witches’ brooms on sharp-flowered rush (Juncus acutiflorus) in Poland. Plant Dis 102. https://doi.org/10.1094/PDIS-03-18-0383-PDN

Lee IM, Gundersen-Rindal DE, Davis RE, Bartoszyk IM (1998) Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int J Syst Bacteriol 48:1153–1169

Lee IM, Davis RE, Gundersen-Rindal DE (2000) Phytoplasma: phyto- pathogenic mollicutes. Annu Rev Microbiol 54:221–255

Maixner M, Ahrens U, Seemüller E (1995) Detection of the German grapevine yellows (Vergilbungskrankheit) MLO in grapevine, alternative hosts and a vector by a specific PCR procedure. Eur J Plant Pathol 101:241–250

Ministry of Agriculture (2011) Twenty-fourth statistical yearbook. Total estimated of agricultural crops area and production in Saudi Arabia: estimated area and production of dates crop by region in Saudi Arabia. Department of Economic Studies and Statistics, Ministry of Agriculture, Riyadh, Saudi Arabia, pp 68–70

Omar AF (2016) Association of ‘Candidatus Phytoplasma cynodontis’ with Bermuda grass white leaf disease and its new hosts in Qassim province, Saudi Arabia. J Plant Interact 11:101–107

Omar AF (2017) Detection and molecular characterization of phytoplasmas associated with vegetable and alfalfa crops in Qassim region. J Plant Interact 12:58–66

Omar AF, Pérez-López E, Al-Jamhan KM, Dumonceaux TJ (2017) First report of a new jojoba (Simmondsia chinensis) witches’ broom disease in Saudi Arabia and its association with infection by a ‘Candidatus Phytoplasma australasiae’ - related phytoplasma strain. Plant Dis 101:1540

Oropeza C, Cordova I, Narvaez M, Harrison N (2002) Palm trunk sampling for DNA extraction and Phytoplasma detection. University of Florida, Florida

Pérez-López E, Luna-Rodríguez M, Olivier CY, Dumonceaux TJ (2016) The underestimated diversity of phytoplasmas in Latin America. Int J Syst Evol Microbiol 66:492–513

Pérez-López E, Omar AF, Al-Jamhan KM, Dumonceaux TJ (2018) Molecular identification and characterization of the new 16SrIX-J and cpn60UT IX-J phytoplasma subgroup associated with chicory bushy stunt disease in Saudi Arabia. International Journal of Systematic and Evolutionary Microbiology. 192. https://doi.org/10.1099/ijsem.0.002530

Schneider B, Seemüller E, Smart CD, Kirkpatrick BC (1995) Phylogenetic classification of plant pathogenic mycoplasmalike organ- isms or phytoplasmas. In: Razin R, Tully JG (eds) Molecular and diagnostic procedures in Mycoplasmology, vol. 1: pp. 369–380. Academic Press, San Diego

Statistical Year Book. (2016). Ministry of agriculture, Vol. 52. Kingdom of Saudi Arabia. https://www.stats.gov.sa/en/877. Accessed November 2017

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

Vázquez-Euán R, Harrison N, Narvaez M, Oropeza C (2011) Occurrence of a 16SrIV group phytoplasma not previously associated with palm species in Yucatan, Mexico. Plant Dis 95:256–262

Wei W, Davis RE, Lee IM, Zhao Y (2007) Computer-simulated RFLP analysis of 16S rRNA genes: identification of ten new phytoplasma groups. Int J Syst Bacteriol 57:1855–1867

Zamharir MG, Boshehri SMS, Khajehzadeh Y (2016) Candidatus phytoplasma fraxini related (16SrRNA-VII) strain associated with date yellows disease in Iran. Aust Plant Dis Notes 11:31

Zhao Y, Wei W, Lee I-M, Shao J, Suo X et al (2009) Construction of an interactive online phytoplasma classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). Int J Syst Evol Microbiol 59:2582–2593

Thông tin
Thông tin xuất bản

Springer Science and Business Media LLC

Thông tin tác giả