<scp>MSL</scp>1 is a mechanosensitive ion channel that dissipates mitochondrial membrane potential and maintains redox homeostasis in mitochondria during abiotic stress

Plant Journal - Tập 88 Số 5 - Trang 809-825 - 2016
Chun Pong Lee1, Grigory Maksaev2, Gregory S. Jensen2, Monika W. Murcha1, Margaret E. Wilson2, Mark D. Fricker3, Rüdiger Hell4, Elizabeth S. Haswell2, A. Harvey Millar1, Lee Sweetlove3
1ARC Centre of Excellence in Plant Energy Biology, University of Western Australia, Bayliss Building M316, 35 Stirling Highway, Crawley, 6009 Western Australia, Australia
2Department of Biology, Washington University in Saint Louis, One Brookings Drive, Mailcode 1137, Saint Louis, MO, 63130 USA
3Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK,
4Department of Plant Molecular Biology, Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 360, D-69120 Heidelberg, Germany

Tóm tắt

SummaryMitochondria must maintain tight control over the electrochemical gradient across their inner membrane to allow ATP synthesis while maintaining a redox‐balanced electron transport chain and avoiding excessive reactive oxygen species production. However, there is a scarcity of knowledge about the ion transporters in the inner mitochondrial membrane that contribute to control of membrane potential. We show that loss of MSL1, a member of a family of mechanosensitive ion channels related to the bacterial channel MscS, leads to increased membrane potential of Arabidopsis mitochondria under specific bioenergetic states. We demonstrate that MSL1 localises to the inner mitochondrial membrane. When expressed in Escherichia coli, MSL1 forms a stretch‐activated ion channel with a slight preference for anions and provides protection against hypo‐osmotic shock. In contrast, loss of MSL1 in Arabidopsis did not prevent swelling of isolated mitochondria in hypo‐osmotic conditions. Instead, our data suggest that ion transport by MSL1 leads to dissipation of mitochondrial membrane potential when it becomes too high. The importance of MSL1 function was demonstrated by the observation of a higher oxidation state of the mitochondrial glutathione pool in msl1‐1 mutants under moderate heat‐ and heavy‐metal‐stress. Furthermore, we show that MSL1 function is not directly implicated in mitochondrial membrane potential pulsing, but is complementary and appears to be important under similar conditions.

Từ khóa


Tài liệu tham khảo

10.1104/pp.010326

10.1016/j.bbabio.2010.02.035

10.1016/S0014-5793(99)01611-7

10.1021/ja990623g

10.1186/1471-2229-14-144

10.1073/pnas.0402040101

10.1126/science.1077945

10.1007/BF00769534

10.1104/pp.113.221200

10.1042/BST0390733

10.1007/s11103-005-5514-7

10.1038/ncomms3137

10.1016/0005-2728(85)90081-7

Duncan O., 2015, Plant Mitochondria, 61, 10.1007/978-1-4939-2639-8_5

10.1529/biophysj.107.123448

10.4161/chan.20998

10.1104/pp.104.046698

10.1104/pp.103.024620

10.1074/mcp.M900325-MCP200

10.1089/ars.2015.6390

10.1021/pr034025j

10.1104/pp.113.230383

10.1146/annurev-arplant-043014-114700

10.1126/science.aac6014

10.1016/j.cub.2005.11.044

10.1104/pp.56.1.13

10.1104/pp.109.137885

10.1104/pp.64.1.115

10.1074/jbc.272.39.24272

10.1104/pp.64.6.948

10.1074/jbc.M009093200

10.1111/j.1432-1033.1994.0907b.x

10.1111/j.1432-1033.1994.0923b.x

10.1111/j.1432-1033.1994.0897b.x

10.1104/pp.111.182352

10.1146/annurev.micro.112408.134106

10.1073/pnas.1323899111

10.1093/pcp/pcn082

10.1074/mcp.M700535-MCP200

10.1093/emboj/18.7.1730

10.1093/emboj/cdf537

10.1104/pp.94.1.54

10.1073/pnas.1213931109

10.1007/978-1-62703-245-2_23

10.1104/pp.010387

10.1093/emboj/cdg011

10.1111/j.1432-1033.1969.tb19633.x

10.1146/annurev.arplant.52.1.561

10.1093/jxb/28.3.607

10.1146/annurev-arplant-042811-105432

10.1104/pp.102.016808

10.1016/j.jmb.2004.09.045

10.1146/annurev.pharmtox.47.120505.105110

10.1146/annurev-biophys-101211-113227

10.1111/j.1432-1033.1974.tb03899.x

10.1104/pp.112.204263

10.1128/MMBR.67.1.66-85.2003

10.1074/jbc.M111.267898

10.1146/annurev.arplant.55.031903.141720

10.1007/s00249-015-1020-2

10.3109/10715769009145682

10.1085/jgp.201110767

10.1006/excr.1994.1038

10.1104/pp.011577

10.1111/j.1365-2818.2008.02030.x

10.1105/tpc.112.096438

10.1089/ars.2015.6266

10.1074/jbc.M408920200

10.1016/S1063-5823(06)58001-9

10.1016/S0006-3495(02)75169-2

10.1007/978-1-59745-365-3_9

10.1080/10715760802582183

10.3389/fpls.2015.01072

10.1016/j.cub.2012.01.027

10.1105/tpc.114.128082

10.1146/annurev.arplant.57.032905.105335

10.1093/jxb/erw100

10.1105/tpc.15.00509

10.1105/tpc.111.088112

10.1074/mcp.M900526-MCP200

10.1074/jbc.M109.059956

10.1371/journal.pone.0001994

Thông tin
Thông tin xuất bản

Plant Journal

Tập 88 Số 5

809-825

Thông tin tác giả