mab-20 encodes Semaphorin-2a and is required to prevent ectopic cell contacts during epidermal morphogenesis in Caenorhabditis elegans

Development (Cambridge) - Tập 127 Số 4 - Trang 755-767 - 2000
Peter J. Roy1,2, Hong Zheng1, Charles E. Warren1, Joseph G. Culotti1,2
1Department of Molecular and Medical Genetics 2 , University of Toronto, Toronto, Ontario M5S 1A8, Canada
2Samuel Lunenfeld Research Institute 1 , Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada

Tóm tắt

ABSTRACT The Semaphorins are a family of secreted and transmembrane proteins known to elicit growth cone repulsion and collapse. We made and characterized a putative null mutant of the C. elegans gene semaphorin-2a (Ce-sema-2a). This mutant failed to complement mutants of mab-20 (Baird, S. E., Fitch, D. H., Kassem, I. A. A. and Emmons, S. W. (1991) Development 113, 515-526). In addition to low-frequency axon guidance errors, mab-20 mutants have unexpected defects in epidermal morphogenesis. Errant epidermal cell migrations affect epidermal enclosure of the embryo, body shape and sensory rays of the male tail. These phenotypic traits are explained by the formation of inappropriate contacts between cells of similar type and suggest that Ce-Sema-2a may normally prevent formation or stabilization of ectopic adhesive contacts between these cells.

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