<i>OsDREB</i> genes in rice, <i>Oryza sativa</i> L., encode transcription activators that function in drought‐, high‐salt‐ and cold‐responsive gene expression

Plant Journal - Tập 33 Số 4 - Trang 751-763 - 2003
Joseph G. Dubouzet1, Yoh Sakuma, Yusuke Ito, Mie Kasuga, Emilyn G. Dubouzet, Setsuko Miura, Motoaki Seki, Kazuo Shinozaki, Kazuo Shinozaki
1Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan

Tóm tắt

SummaryThe transcription factors DREBs/CBFs specifically interact with the dehydration‐responsive element/C‐repeat (DRE/CRT) cis‐acting element (core motif: G/ACCGAC) and control the expression of many stress‐inducible genes in Arabidopsis. In rice, we isolated five cDNAs for DREB homologs: OsDREB1A, OsDREB1B, OsDREB1C, OsDREB1D, and OsDREB2A. Expression of OsDREB1A and OsDREB1B was induced by cold, whereas expression of OsDREB2A was induced by dehydration and high‐salt stresses. The OsDREB1A and OsDREB2A proteins specifically bound to DRE and activated the transcription of the GUS reporter gene driven by DRE in rice protoplasts. Over‐expression of OsDREB1A in transgenic Arabidopsis induced over‐expression of target stress‐inducible genes of Arabidopsis DREB1A resulting in plants with higher tolerance to drought, high‐salt, and freezing stresses. This indicated that OsDREB1A has functional similarity to DREB1A. However, in microarray and RNA blot analyses, some stress‐inducible target genes of the DREB1A proteins that have only ACCGAC as DRE were not over‐expressed in the OsDREB1A transgenic Arabidopsis. The OsDREB1A protein bound to GCCGAC more preferentially than to ACCGAC whereas the DREB1A proteins bound to both GCCGAC and ACCGAC efficiently. The structures of DREB1‐type ERF/AP2 domains in monocots are closely related to each other as compared with that in the dicots. OsDREB1A is potentially useful for producing transgenic monocots that are tolerant to drought, high‐salt, and/or cold stresses.

Từ khóa


Tài liệu tham khảo

10.1007/BF00029852

Bechtold N., 1993, In planta Agrobacterium‐mediated gene transfer by infiltration of adult Arabidopsis thaliana plants, C. R. Acad. Sci. Paris, Life Sci., 316, 1194

10.1073/pnas.94.11.5961

10.1007/BF00020010

Elliott R.C., 1996, AINTEGUMENTA, an APETALA2‐like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth, Plant Cell, 8, 155

Finkelstein R.R., 1998, The Arabidopsis acid response locus ABI4 encodes an APETALA2 domain protein, Plant J., 10, 1043

10.1126/science.289.5477.295

10.1105/tpc.12.3.393

10.1093/nar/15.21.8693

10.1104/pp.124.4.1854

10.1046/j.1365-313x.1998.00310.x

Van Der Graaff E., 2000, Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana, Development, 127, 4971, 10.1242/dev.127.22.4971

10.1046/j.1365-313X.1995.07060913.x

10.1073/pnas.96.26.15348

10.1146/annurev.arplant.47.1.377

10.1104/pp.010548

Jaglo‐Ottosen K.R., 1998, Arabidopsis CBF1 over‐expression induces COR genes and enhances freezing tolerance, Science, 280, 104, 10.1126/science.280.5360.104

10.1007/BF00049344

Jofuku K.D., 1994, Control of Arabidopsis flower and seed development by the homeotic gene, APETALA2, Plant Cell, 6, 1211

10.1093/nar/27.2.470

10.1038/7036

10.1105/tpc.10.8.1391

10.1093/emboj/18.16.4455

10.1093/oxfordjournals.pcp.a028913

10.1023/A:1006321900483

10.1073/pnas.94.13.7076

10.1016/S0014-5793(98)00116-1

Park J.M., 2001, Over‐expression of the tobacco Tsi1 gene encoding an EREBP/AP2‐type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco, Plant Cell, 13, 1035

10.1006/bbrc.2001.6299

10.1105/tpc.13.1.61

10.1046/j.1365-313X.2002.01359.x

10.1104/pp.115.2.327

10.1016/S1369-5266(00)00067-4

10.1006/bbrc.1998.9267

10.1101/gad.12.23.3703

10.1073/pnas.94.3.1035

10.1146/annurev.arplant.50.1.571

Urao T., 1993, An Arabidopsis myb homologue is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence, Plant Cell, 5, 1529

Wilson K., 1996, A dissociation insertion causes a semidominant mutation that increases expression of TINY, an Arabidopsis gene related to APETALA2, Plant Cell, 8, 659

Yamaguchi‐Shinozaki K., 1994, A novel cis‐acting element in an Arabidopsis gene is involved in responsiveness to drought, low‐temperature or high‐salt stress, Plant Cell, 6, 251