<i>In vitro</i> assessment of electrospun polyamide‐6 scaffolds for esophageal tissue engineering

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 107 Số 2 - Trang 253-268 - 2019
Margarita Zhuravleva1, Zarema E. Gilazieva1, T. Е. Grigoriev2, А. Д. Шепелев2, Т. Х. Тенчурин2, Roman Kamyshinsky2, С. В. Крашенинников2, С. В. Орлов3, Gina Caralogli3, Svetlana Archipova1, Mark Holterman4, M. O Mavlikeev1, Р. В. Деев5,6, С. Н. Чвалун2, Paolo Macchiarini1
1Laboratory of Bioengineering and Regenerative Medicine (BioReM), Kazan Federal University, Kazan, Russia
2National Research Center Kurchatov Institute, Moscow, Russia
3Scientific Research Institute of Medical Primatology, Sochi, Russia
4University of Illinois College of Medicine Peoria, Peoria, Illinois
5Human Stem Cells Institute, Moscow, Russia; Ryazan State Medical University Ryazan Russia
6Ryazan State Medical University, Ryazan, Russia

Tóm tắt

AbstractArtificial tissue‐engineered grafts offer a potential alternative to autologous tissue grafts for patients, which can be traumatic. After decellularizing Papio hamadryas esophagus and studying the morphology and physical properties of the extracellular matrix (ECM), we generated electrospun polyamide‐6 based scaffolds to mimic it. The scaffolds supported a greater mechanical load than the native ECM and demonstrated similar 3D microstructure, with randomly aligned fibers, 90% porosity, 29 μm maximal pore size, and average fiber diameter of 2.87 ± 0.95 µm. Biocompatibility studies showed that human adipose‐ and bone marrow‐derived mesenchymal stromal cells (AD‐MSC and BMD‐MSC) adhered to the scaffold surface and showed some proliferation: scaffold cell coverage was 25% after 72 h of incubation when seeded with 1000 cells/mm2; cells elongated processes along the polyamide‐6, although they flattened 1.67–4 times less than on cell culture plastic. Human umbilical vein endothelial cells, however, showed poor adherence and proliferation. We thus provide in vitro evidence that polyamide‐6 scaffolds approximating the esophageal biomechanics and 3D topography of nonhuman primates may provide a biocompatible substrate for both AD‐MSC and BMD‐MSCs, supporting their adhesion and survival to some degree. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 253–268, 2019.

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Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 107 Số 2

253-268

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