<i>In vitro</i>anti-inflammatory effects of citrulline on peritoneal macrophages in Zucker diabetic fatty rats

British Journal of Nutrition - Tập 113 Số 1 - Trang 120-124 - 2015
C. Breuillard1, Sandra Bonhomme1, Rémy Couderc1,2, Luc Cynober1,3, Jean‐Pascal De Bandt1,3
1Laboratoire de Biologie de la Nutrition, EA 4466, Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
2Service de Biochimie, Hôpital Armand-Trousseau, AP-HP, Paris, France
3Service de Biochimie, Hôpital Cochin, Hôpitaux Universitaires Paris Centre, AP-HP, Paris, France

Tóm tắt

In type 2 diabetes (T2D) macrophage dysfunction increases susceptibility to infection and mortality. This may result from the associated decreased plasma concentration of arginine, an amino acid that plays an important role in immunity.In vitro, increasing arginine availability leads to an improvement in macrophage function; however, arginine supplementation in diabetic obese patients may be detrimental. The aim of the present study was to assessin vitrowhether citrulline, an arginine precursor, could replace arginine in the regulation of macrophage function under a condition of diabetes and obesity. Peritoneal macrophages from diabetic obese or lean rats were incubated for 6 h in an arginine-free medium, in the presence of increasing citrulline concentrations (0·1, 0·5, 1 or 2 mmol/l). Cytokine and NO production was determined. Peritoneal macrophages from either lean or diabetic obese rats produced NO, and at higher levels in the cells from lean rats. In diabetic obese rats, TNF-α production decreased with increasing citrulline concentrations, but was higher than that in the cells from lean rats. In contrast, IL-6 production increased with increasing citrulline concentrations. The present experiment shows that citrulline is effectively used for NO production and regulates cytokine production in macrophages from diabetic obese rats. This effect warrantsin vivoevaluation in T2D-related inflammation.

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Thông tin xuất bản

British Journal of Nutrition

Tập 113 Số 1

120-124

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