Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions

Proceedings of the National Academy of Sciences of the United States of America - Tập 97 Số 21 - Trang 11632-11637 - 2000
Yuichi Uno1, Takashi Furihata1, Hiroshi Abe1, Riichiro Yoshida1, Kazuo Shinozaki1, Kazuko Yamaguchi‐Shinozaki1
1Biological Resources Division, Japan International Research Center for Agricultural Science (JIRCAS), Ministry of Agriculture, Forestry, and Fisheries, Ohwashi 1–2, Tsukuba, Ibaraki 305-8686, Japan; and Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3–1-1, Koyadai, Tsukuba, Ibaraki 305-0074, Japan

Tóm tắt

The induction of the dehydration-responsive Arabidopsis gene, rd29B , is mediated mainly by abscisic acid (ABA). Promoter analysis of rd29B indicated that two ABA-responsive elements (ABREs) are required for the dehydration-responsive expression of rd29B as cis-acting elements. Three cDNAs encoding basic leucine zipper (bZIP)-type ABRE-binding proteins were isolated by using the yeast one-hybrid system and were designated AREB1, AREB2, and AREB3 (ABA-responsive element binding protein). Transcription of the AREB1 and AREB2 genes is up-regulated by drought, NaCl, and ABA treatment in vegetative tissues. In a transient transactivation experiment using Arabidopsis leaf protoplasts, both the AREB1 and AREB2 proteins activated transcription of a reporter gene driven by ABRE. AREB1 and AREB2 required ABA for their activation, because their transactivation activities were repressed in aba2 and abi1 mutants and enhanced in an era1 mutant. Activation of AREBs by ABA was suppressed by protein kinase inhibitors. These results suggest that both AREB1 and AREB2 function as transcriptional activators in the ABA-inducible expression of rd29B , and further that ABA-dependent posttranscriptional activation of AREB1 and AREB2, probably by phosphorylation, is necessary for their maximum activation by ABA. Using cultured Arabidopsis cells, we demonstrated that a specific ABA-activated protein kinase of 42-kDa phosphorylated conserved N-terminal regions in the AREB proteins.

Từ khóa


Tài liệu tham khảo

D Bonetta, P McCourt Trends Plant Sci 3, 231–235 (1998).

E Grill, A Himmelbach Curr Opin Plant Biol 1, 412–418 (1998).

J Leung, J Giraudat Annu Rev Plant Physiol Plant Mol Biol 49, 199–222 (1998).

M J Guiltinan, W R Marcotte, R S Quatrano Science 250, 267–271 (1990).

J Mundy, K Yamaguchi-Shinozaki, N-H Chua Proc Natl Acad Sci USA 87, 406–410 (1990).

A E Menkes, U Schindler, A R Cashmore Trends Biochem Sci 20, 506–510 (1995).

L M Shen, T-H D Ho Plant Cell 7, 295–307 (1995).

T Hobo, M Asada, Y Kowyama, T Hattori Plant J 19, 679–689 (1999).

M Koornneef, G Reuling, C M Karssen Physiol Plant 61, 377–383 (1984).

D R McCarty, T Hattori, C B Carson, V Vasil, M Lazar, I K Vasil Cell 66, 895–905 (1991).

J Giraudat, B M Hauge, C Valon, J Smalle, F Parcy, H M Goodman Plant Cell 4, 1251–1261 (1992).

V Vasil, W R Marcotte, L Rosenkrans, S M Cocciolone, I K Vasil, R S Quatrano, D R McCarty Plant Cell 7, 1511–1518 (1995).

P K Busk, A J Jensen, M Pages Plant J 11, 1285–1295 (1997).

J Leung, M Bouvier-Durand, P-C Morris, D Guerrier, F Chefdor, J Giraudat Science 264, 1448–1452 (1994).

K Meyer, M P Leube, E Grill Science 264, 1452–1455 (1994).

J Leung, S Merlot, J Giraudat Plant Cell 9, 759–771 (1997).

S Cutler, M Ghassemian, D Bonetta, S Cooney, P McCourt Science 273, 1239–1241 (1996).

K Yamaguchi-Shinozaki, K Shinozaki Plant Cell 6, 251–264 (1994).

M Koornneef, M L Jorna, D L C Brinkhorst-van der Swan, C M Karssen Theor Appl Genet 61, 385–393 (1992).

H Abe, K Yamaguchi-Shinozaki, T Urao, T Iwasaki, D Hosokawa, K Shinozaki Plant Cell 9, 1859–1868 (1997).

Q Liu, M Kasuga, Y Sakuma, H Abe, S Miura, K Yamaguchi-Shinozaki, K Shinozaki Plant Cell 10, 1391–1406 (1998).

T Mizoguchi, K Yamaguchi-Shinozaki, N Hayashida, H Kamada, K Shinozaki Plant J 21, 279–289 (1993).

S Usami, H Banno, Y Ito, R Nishihama, Y Machida Proc Natl Acad Sci USA 92, 8660–8664 (1995).

U Schindler, W Terzaghi, H Beckmann, T Kadesch, A R Cashmore EMBO J 11, 1275–1289 (1992).

M Seki, P Carninci, Y Nishiyama, Y Hayashizaki, K Shinozaki Plant J 15, 707–720 (1998).

S Y Kim, H-J Chung, T L Thomas Plant J 11, 1237–1251 (1997).

K Nordin, T Vahala, T Palva Plant Mol Biol 21, 641–653 (1993).

K Skriver, F L Olsen, J C Rogers, J Mundy Proc Natl Acad Sci USA 88, 7266–7270 (1991).

Q Shen, P H Zhang, T H Ho Plant Cell 8, 1107–1119 (1996).

G Lu, A L Paul, D R McCarty, R J Ferl Plant Cell 8, 847–857 (1996).

T Hobo, Y Kowyama, T Hattori Proc Natl Acad Sci USA 96, 15348–15353 (1999).

H Choi, J Hong, J Ha, J Kang, S Y Kim J Biol Chem 275, 1723–1730 (2000).

R R Finkelstein, T J Lynch Plant Cell 12, 599–609 (2000).

Thông tin
Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 97 Số 21

11632-11637

Thông tin tác giả