Development of microsatellite markers for Aulonemia aristulata (Poaceae) and cross‐amplification in other bamboo species

American Journal of Botany - Tập 98 Số 4 - 2011
A. G. de Abreu1, Maria Tereza Grombone‐Guaratini2, Mariza Monteiro1, José Baldin Pinheiro3, Antônio Fernando Caetano Tombolato4, Maria Imaculada Zucchi1
1APTA Pólo Centro-Sul, Rodovia SP 127, km 30, CP 28, Piracicaba, SP, Brazil 13400-970
2Instituto de Botânica, Núcleo de Pesquisa em Ecologia, CP 3005, São Paulo, SP, Brazil 01061-970
3Departamento de genética, Universidade de São Paulo, Av. Pádua Dias, 11, CP 83, Piracicaba, SP, Brazil 13400‐970
4Instituto Agronômico, Avenida Barão de Itapura, 1481, CP 28, Campinas, SP, Brazil 13020-902

Tóm tắt

• Premise of the study: Microsatellite primers were developed for Aulonemia aristulata, an endangered species of economic interest, to further describe its genetic variability and population structure. We also tested cross‐amplification in 18 other bamboo species.

• Methods and Results: Using an enrichment genomic library, 13 microsatellite loci were isolated and characterized in A. aristulata. Seven of these loci were polymorphic. Twelve markers were cross‐amplified in at least ten of the tested bamboo species.

• Conclusions: These markers will be useful for studies on the genetic diversity and structure of A. aristulata, which are important for future conservation, management and breeding programs of this species.

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American Journal of Botany

Tập 98 Số 4

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