[3H]Thymidine autoradiographic and alkaline phosphatase histochemical studies of intestinal metaplasia of the human stomach
Tóm tắt
The relationship between cell proliferation and enzyme activity in intestinal metaplasia of the human stomach was studied using a combined method of [3H]thymidine autoradiography and alkaline phosphatase histochemistry on the same section. Three types of intestinal metaplasia were observed depending on variations in both enzymatic activity and isotope labelling. One type shows alkaline phosphatase-positive cells along the entire length of the glands with [3H]thymidine-labelled cells localized only at the bottom of the glands, resembling the duodenum. In another type of intestinal metaplasia, alkaline phosphatase-positive cells are present on the surface and/or upper half of the glands with mitotically active cells occupying the lower part of the glands. The third variety of intestinal metaplasia is characterized by the absence of alkaline-phosphatase activity and [3H]thymidine-labelled cells present in an extended zone in the lower half of the glands. Differences in labelling patterns of [3H]thymidine and the activity of marker enzyme in various types of intestinal metaplasia seem to reflect variations in cell differentiation during intestinalization of gastric mucosa.
Tài liệu tham khảo
Abe, M., Ohuchi, N. &Sakano, H. (1974) Enzyme histo- and bio-chemistry of intestinalized gastric mucosa.Acta histochem. cytochem. 7, 282–8.
Burston, M. S. (1958) Histochemical comparison of naphtol-AS-phosphates for the demonstration of phosphatases.J. natn. Cancer Inst. 20, 601–15.
Hattori, T. &Fujita, S. (1979) Tritiated thymidine autoradiographic study on histogenesis and spreading of intestinal metaplasia in human stomach.Path. Res. Pract. 164, 224–37.
Higuchi, T. &Suto, H. (1971)3H-thymidine autoradiographic studies on the human gastric mucosa of chronic gastritis.Jap. J. Gastroent. 68, 160–75 (in Japanese with English abstract).
Jass, J. &Filipe, M. I. (1979) A variant of intestinal metaplasia associated with gastric carcinoma: a histochemical study.Histopathology 3, 191–9.
Kawachi, T., Kogure, K., Tanaka, N., Tokunaga, A., Sugimura, T., Koyama, Y., Kanasugi, K., Hirota, T. &Sano, R. (1974). Studies of intestinal metaplasia in the gastric mucosa by detection of disaccharidases with Tes-tape.J. natn. Cancer Inst. 53, 19–30.
Kumagae, K., Sasajima, K., Hashimoto, M., Numajiri, H., Tokunaga, A., Tanaka, N., Shirota, A. &Asano, G. (1981) Cytochemical localization of alkaline phosphatase in intestinal metaplasia of the human stomach.Histochem. J. 13, 57–62.
Matsukura, N., Suzuki, K., Kawachi, T., Aoyama, M., Sugimura, T., Kitaoka, H., Numajiri, H., Shirota, A., Itabashi, M. &Hirota, T. (1980) Distribution of marker enzymes and mucin in intestinal metaplasia in human stomach and relation of complete and incomplete types of intestinal metaplasia to minute gastric carcinomas.J. natn. Cancer Inst. 65, 231–40.
Sugimura, T., Kawachi, T., Kogure, K., Tanaka, N., Kazama, S. &Koyama, Y. (1971) A novel method for detecting intestinal metaplasia of the stomach with Tes-tape.Gann. 62, 237–8.
Teglbjaerg, P. S. &Nielsen, H. O. (1978) ‘Small intestinal’ and ‘colonic type’ intestinal metaplasia of the human stomach.Acta path. microbiol. scand. 86, 351–5.