γH2AX: a sensitive molecular marker of DNA damage and repair
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Karagiannis TC, El-Osta A . Double-strand breaks: signaling pathways and repair mechanisms. Cell Mol Life Sci 2004; 61: 2137–2147.
Sedelnikova OA, Pilch DR, Redon C, Bonner WM . Histone H2AX in DNA damage and repair. Cancer Biol Ther 2003; 2: 233–235.
Khanna KK, Jackson SP . DNA double-strand breaks: signaling, repair and the cancer connection. Nat Genet 2001; 27: 247.
Pastink A, Lohman PHM . Repair and consequences of double-strand breaks in DNA. Mutat Res 1999; 428: 141–156.
Bewersdorf J, Bennett BT, Knight KL . H2AX chromatin structures and their response to DNA damage revealed by 4Pi microscopy. Proc Natl Acad Sci 2006; 103: 18137–18142.
Redon C, Pilch D, Rogakou E, Sedelnikova O, Newrock K, Bonner W . Histone H2A variants H2AX and H2AZ. Curr Opin Genet Dev 2002; 12: 162–169.
Sedelnikova OA, Rogakou EP, Panyutin IG, Bonner WM . Quantitative detection of (125) IdU-induced DNA double-strand breaks with gamma-H2AX antibody. Radiat Res 2002; 158: 486–492.
Rogakou EP, Boon C, Redon C, Bonner WM . Megabase chromatin domains involved in DNA double-strand breaks in vivo. J Cell Biol 1999; 146: 905–916.
Rothkamm K, Lobrich M . Evidence for a lack of DNA double-strand break repair in human cells exposed to very low X-ray doses. Proc Natl Acad Sci USA 2003; 100: 5057–5062.
Chen HT, Bhandoola A, Difilippantonio MJ, Zhu J, Brown MJ, Tai X et al. Response to RAG-mediated V(D)J cleavage by NBS1 and {gamma}-H2AX. Science 2000; 290: 1962–1964.
Ayoub N, Jeyasekharan AD, Bernal JA, Venkitaraman AR . HP1-beta mobilization promotes chromatin changes that initiate the DNA damage response. Nature 2008; 453: 682–686.
Kinner A, Wu W, Staudt C, Iliakis G . Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Res 2008; 36: 5678–5694.
Bhogal N, Jalali F, Bristow RG . Microscopic imaging of DNA repair foci in irradiated normal tissues. Int J Radiat Biol 2009; 85: 732–746.
Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM . DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998; 273: 5858–5868.
Stucki M, Clapperton JA, Mohammad D, Yaffe MB, Smerdon SJ, Jackson SP . MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks. Cell 2005; 123: 1213–1226.
Sonoda E, Hochegger H, Saberi A, Taniguchi Y, Takeda S . Differential usage of non-homologous end-joining and homologous recombination in double strand break repair. DNA Repair 2006; 5: 1021–1029.
Shrivastav M, De Haro LP, Nickoloff JA . Regulation of DNA double-strand break repair pathway choice. Cell Res 2008; 18: 134–147.
Burma S, Chen BPC, Chen DJ . Role of non-homologous end joining (NHEJ) in maintaining genomic integrity. DNA Repair 2006; 5: 1042–1048.
Rothkamm K, Kruger I, Thompson LH, Lobrich M . Pathways of DNA double-strand break repair during the mammalian cell cycle. Mol Cell Biol 2003; 23: 5706–5715.
Natarajan AT, Berni A, Marimuthu KM, Palitti F . The type and yield of ionising radiation induced chromosomal aberrations depend on the efficiency of different DSB repair pathways in mammalian cells. Mutat Res 2008; 642: 80–85.
Takata M, Sasaki MS, Sonoda E, Morrison C, Hashimoto M, Utsumi H et al. Homologous recombination and non-homologous end-joining pathways of DNA double-strand break repair have overlapping roles in the maintenance of chromosomal integrity in vertebrate cells. EMBO J 1998; 17: 5497–5508.
West SC . Molecular views of recombination proteins and their control. Nat Rev Mol Cell Biol 2003; 4: 435.
Valerie K, Povirk LF . Regulation and mechanisms of mammalian double-strand break repair. Oncogene 2003; 22: 5792–5812.
Johnson RD, Jasin M . Sister chromatid gene conversion is a prominent double-strand break repair pathway in mammalian cells. EMBO J 2000; 19: 3398–3407.
Bassing C, Alt F . H2AX may function as an anchor to hold broken chromosomal DNA ends in close proximity. Cell Cycle 2004; 3: 149–153.
Chapman JR, Jackson SP . Phospho-dependent interactions between NBS1 and MDC1 mediate chromatin retention of the MRN complex at sites of DNA damage. EMBO Rep 2008; 9: 795–801.
Bouquet F, Muller C, Salles B . The loss of gammaH2AX signal is a marker of DNA double strand breaks repair only at low levels of DNA damage. Cell Cycle 2006; 5: 1116–1122.
Banath JP, MacPhail SH, Olive PL . Radiation sensitivity, H2AX phosphorylation, and kinetics of repair of DNA strand breaks in irradiated cervical cancer cell lines. Cancer Res 2004; 64: 7144–7149.
Xie A, Puget N, Shim I, Odate S, Jarzyna I, Bassing C et al. Control of sister chromatid recombination by histone H2AX. Mol Cell 2004; 16: 1017–1025.
Ström L, Lindroos H, Shirahige K, Sjögren C . Postreplicative recruitment of cohesin to double-strand breaks is required for DNA repair. Mol Cell 2004; 16: 1003–1015.
Unal E, Arbel-Eden A, Sattler U, Shroff R, Lichten M, Haber J et al. DNA damage response pathway uses histone modification to assemble a double-strand break-specific cohesin domain. Mol Cell 2004; 16: 991–1002.
Soutoglou E, Dorn JF, Sengupta K, Jasin M, Nussenzweig A, Ried T et al. Positional stability of single double-strand breaks in mammalian cells. Nat Cell Biol 2007; 9: 675–682.
Karagiannis TC, El-Osta A . Epigenetic changes activate widespread signals in response to double-strand breaks. Cancer Biol Ther 2004; 3: 617–623.
Celeste A, Difilippantonio S, Difilippantonio MJ, Fernandez-Capetillo O, Pilch DR, Sedelnikova OA et al. H2AX haploinsufficiency modifies genomic stability and tumor susceptibility. Cell 2003; 114: 371–383.
Fernandez-Capetillo O, Chen H-T, Celeste A, Ward I, Romanienko PJ, Morales JC et al. DNA damage-induced G2-M checkpoint activation by histone H2AX and 53BP1. Nat Cell Biol 2002; 4: 993.
Lees-Miller SP, Sakaguchi K, Ullrich SJ, Appella E, Anderson CW . Human DNA-activated protein kinase phosphorylates serines 15 and 37 in the amino-terminal transactivation domain of human p53. Mol Cell Biol 1992; 12: 5041–5049.
Karlsson K, Stenerlöw B . Focus formation of DNA repair proteins in normal and repair-deficient cells irradiated with high-LET ions. Radiat Res 2004; 161: 517–527.
Leatherbarrow EL, Harper JV, Cucinotta FA, O’Neill P . Induction and quantification of g-H2AX foci following low and high LET-irradiation. Int J Radiat Biol 2006; 82: 111–118.
Bakkenist CJ, Kastan MB . DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 2003; 421: 499–506.
Abraham R . Checkpoint signaling: epigenetic events sound the DNA strand-breaks alarm to the ATM protein kinase. BioEssays 2003; 25: 627–630.
Pilch DR, Sedelnikova OA, Redon C, Celeste A, Nussenzweig A, Bonner WM . Characteristics of gamma-H2AX foci at DNA double strand breaks sites. Biochem Cell Biol 2003; 81: 123–129.
Paull TT . A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage. Curr Biol 2000; 10: 886–895.
Stiff T, O’Driscoll M, Rief N, Iwabuchi K, Lobrich M, Jeggo PA . ATM and DNA-PK Function Redundantly to Phosphorylate H2AX after Exposure to Ionizing Radiation. Cancer Res 2004; 64: 2390–2396.
Falck J, Coates J, Jackson SP . Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature 2005; 434: 605–611.
Tanaka H, Arakawa H, Yamaguchi T, Shiraishi K, Fukuda S, Matsui K et al. A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage. Nature 2000; 404: 42–49.
Stiff T, Walker S, Cerosaletti K, Goodarzi A, Petermann E, Concannon P et al. ATR-dependent phosphorylation and activation of ATM in response to UV treatment or replication fork stalling. EMBO J 2006; 25: 5775–5782.
Kurz EU, Lees-Miller SP . DNA damage-induced activation of ATM and ATM-dependent signaling pathways. DNA Repair 2004; 3: 889–900.
Whalen MK, Gurai SK, Zahed-Kargaran H, Pluth JM . Specific ATM-mediated phosphorylation dependent on radiation quality. Radiat Res 2008; 170: 353–364.
Costes S, Boissière A, Ravani S, Romano R, Parvin B, Barcellos-Hoff M . Imaging features that discriminate between foci induced by high- and low-LET radiation in human fibroblasts. Radiat Res 2006; 165: 505–515.
Painter R . Radiation sensitivity and cancer in ataxia-telangiectasiaa. Ann N Y Acad Sci 1985; 459: 382–386.
Zhou B-BS, Elledge SJ . The DNA damage response: putting checkpoints in perspective. Nature 2000; 408: 433.
Bartek J, Falck J, Lukas J . Chk2 kinase [mdash] a busy messenger. Nat Rev Mol Cell Biol 2001; 2: 877–886.
Savic V, Yin B, Maas NL, Bredemeyer AL, Carpenter AC, Helmink BA et al. Formation of dynamic [gamma]-H2AX domains along broken DNA strands is distinctly regulated by ATM and MDC1 and dependent upon H2AX densities in chromatin. Mol Cell 2009; 34: 298–310.
Markova E, Schultz N, Belyaev IY . Kinetics and dose-response of residual 53BP1/gamma-H2AX foci: co-localization, relationship with DSB repair and clonogenic survival. Int J Radiat Biol 2007; 83: 319–329.
Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 2006; 439: 497–501.
Simonsson M, Qvarnström F, Nyman J, Johansson K, Garmo H, Turesson I . Low-dose hypersensitive gammaH2AX response and infrequent apoptosis in epidermis from radiotherapy patients. Radiother Oncol 2008; 88: 388–397.
Sedelnikova OA, Horikawa I, Zimonjic DB, Popescu NC, Bonner WM, Barrett JC . Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks. Nat Cell Biol 2004; 6: 168–170.
Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S et al. Gamma H2AX and cancer. Nat Rev Cancer 2008; 8: 957–967.
Sedelnikova OA, Bonner WM . gamma H2AX in cancer cells: a potential biomarker for cancer diagnostics, prediction and recurrence. Cell Cycle 2006; 5: 2909–2913.
Gorgoulis VG, Vassiliou L-VF, Karakaidos P, Zacharatos P, Kotsinas A, Liloglou T et al. Activation of the DNA damage checkpoint and genomic instability in human precancerous lesions. Nature 2005; 434: 907–913.
Olive PL, Banath JP . Phosphorylation of histone H2AX as a measure of radiosensitivity. Int J Radiat Oncol Biol Phys 2004; 58: 331–335.
Celeste A . Histone H2AX phosphorylation is dispensable for the initial recognition of DNA breaks. Nat Cell Biol 2003; 5: 675–679.
Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K et al. DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 2005; 434: 864–870.
Kato TA, Nagasawa H, Weil MM, Genik PC, Little JB, Bedford JS . Gamma-H2AX Foci after Low-Dose-Rate Irradiation Reveal Atm Haploinsufficiency in Mice. Radiat Res 2006; 166: 47–54.
Watters GP, Smart DJ, Harvey JS, Austin CA . H2AX phosphorylation as a genotoxicity endpoint. Mutat Res 2009; 679: 50–58.
Redon CE, Dickey JS, Bonner WM, Sedelnikova OA . gamma]-H2AX as a biomarker of DNA damage induced by ionizing radiation in human peripheral blood lymphocytes and artificial skin. Adv Space Res 2009; 43: 1171–1178.
Asaad N, Zeng Z, Guan J, Thacker J, Iliakis G . Homologous recombination as a potential target for caffeine radiosensitization in mammalian cells: reduced caffeine radiosensitization in XRCC2 and XRCC3 mutants. Oncogene 2000; 19: 5788–5800.
Paull T, Lee J . The Mre11/Rad50/Nbs1 complex and its role as a DNA double-strand break sensor for ATM. Cell Cycle 2005; 4: 737–740.
Fernandez-Capetillo O, Lee A, Nussenzweig M, Nussenzweig A . H2AX: the histone guardian of the genome. DNA Repair 2004; 3: 959–967.
Schultz LB, Chehab NH, Malikzay A, Halazonetis TD . p53 binding protein 1 (53BP1) is an early participant in the cellular response to DNA double-strand breaks. J Cell Biol 2000; 151: 1381–1390.
Andegeko Y . Nuclear retention of ATM at sites of DNA double strand breaks. J Biol Chem 2001; 276: 38224–38230.
Dellaire G, Bazett-Jones D . Beyond repair foci: subnuclear domains and the cellular response to DNA damage. Cell Cycle 2007; 6: 1864–1872.
Marti TM, Hefner E, Feeney L, Natale V, Cleaver JE . H2AX phosphorylation within the G1 phase after UV irradiation depends on nucleotide excision repair and not DNA double-strand breaks. Proc Natl Acad Sci 2006; 103: 9891–9896.
Ichijima Y, Sakasai R, Okita N, Asahina K, Mizutani S, Teraoka H . Phosphorylation of histone H2AX at M phase in human cells without DNA damage response. Biochem Biophys Res Commun 2005; 336: 807–812.
Fernandez-Capetillo O, Mahadevaiah SK, Celeste A, Romanienko PJ, Camerini-Otero RD, Bonner WM et al. H2AX is required for chromatin remodeling and inactivation of sex chromosomes in male mouse meiosis. Dev Cell 2003; 4: 497–508.
Jeffers LJ, Coull BJ, Stack SJ, Morrison CG . Distinct BRCT domains in Mcph1//Brit1 mediate ionizing radiation-induced focus formation and centrosomal localization. Oncogene 2007; 27: 139–144.
Liu SK, Olive PL, Bristow RG . Biomarkers for DNA DSB inhibitors and radiotherapy clinical trials. Cancer Metastasis Rev 2008; 27: 445–458.
Downs J, Allard S, Jobin-Robitaille O, Javaheri A, Auger A, Bouchard N et al. Binding of chromatin-modifying activities to phosphorylated histone H2A at DNA damage sites. Mol Cell 2004; 16: 979–990.
Morrison A, Highland J, Krogan N, Arbel-Eden A, Greenblatt J, Haber J et al. INO80 and gamma-H2AX interaction links ATP-dependent chromatin remodeling to DNA damage repair. Cell 2004; 119: 767–775.
Ikura T, Tashiro S, Kakino A, Shima H, Jacob N, Amunugama R et al. DNA damage-dependent acetylation and ubiquitination of H2AX enhances chromatin dynamics. Mol Cell Biol 2007; 27: 7028–7040.
Chowdhury D, Keogh M-C, Ishii H, Peterson CL, Buratowski S, Lieberman J . gamma]-H2AX dephosphorylation by protein phosphatase 2A facilitates DNA double-strand break repair. Mol Cell 2005; 20: 801–809.
Nakada S, Chen G, Gingras A, Durocher D . PP4 is a gamma H2AX phosphatase required for recovery from the DNA damage checkpoint. EMBO Rep 2008; 9: 1019–1026.
Altaf M, Auger A, Covic M, Côté J . Connection between histone H2A variants and chromatin remodeling complexes. Biochem Cell Biol 2009; 87: 35–50.
Kusch T, Florens L, MacDonald WH, Swanson SK, Glaser RL, Yates III JR et al. Acetylation by Tip60 is required for selective histone variant exchange at DNA lesions. Science 2004; 306: 2084–2087.
Huertas D, Sendra R, Muñoz P . Chromatin dynamics coupled to DNA repair. Epigenetics 2009; 4: 31–42.
Limoli CL, Giedzinski E, Bonner WM, Cleaver JE . UV-induced replication arrest in the xeroderma pigmentosum variant leads to DNA double-strand breaks, gH2AX formation, and Mre11 relocalization. Proc Natl Acad Sci USA 2002; 99: 233–238.
Ward IM, Minn K, Chen J . UV-induced ataxia-telangiectasia-mutated and Rad3-related (ATR) activation requires replication stress. J Biol Chem 2004; 279: 9677–9680.
Karagiannis TC, Harikrishnan KN, El-Osta A . Disparity of histone deacetylase inhibition on repair of radiation-induced DNA damage on euchromatin and constitutive heterochromatin compartments. Oncogene 2007; 26: 3963–3971.
Goodarzi AA, Noon AT, Deckbar D, Ziv Y, Shiloh Y, Löbrich M et al. ATM signaling facilitates repair of DNA double-strand breaks associated with heterochromatin. Mol Cell 2008; 31: 167–177.
Cowell IG . gammaH2AX foci form preferentially in euchromatin after ionising-radiation. PLoS ONE 2007; 2: e1057.
Kato T, Okayasu R, Bedford J . Signatures of DNA double strand breaks produced in irradiated G1 and G2 cells persist into mitosis. J Cell Physiol 2009; 219: 760–765.
Prise KM, Folkard M, Michael BB . A review of the bystander effect and its implications for low-dose exposure. Radiat Prot Dosimetry 2003; 104: 347–355.
Nagasawa H, Little J . Bystander effect for chromosomal aberrations induced in wild-type and repair deficient CHO cells by low fluences of alpha particles. Mutat Res 2002; 508: 121–129.
Schwartz JL . Variability: the common factor linking low dose-induced genomic instability, adaptation and bystander effects. Mutat Res 2007; 616: 196–200.
Mothersill C, Seymour C . Radiation-induced bystander effects: evidence for an adaptive response to low dose exposures? Dose Response 2006; 4: 283–290.
Burdak-Rothkamm S, Short SC, Folkard M, Rothkamm K, Prise KM . ATR-dependent radiation-induced [gamma]H2AX foci in bystander primary human astrocytes and glioma cells. Oncogene 2006; 26: 993–1002.
Sokolov MV, Smilenov LB, Hall EJ, Panyutin IG, Bonner WM, Sedelnikova OA . Ionizing radiation induces DNA double-strand breaks in bystander primary human fibroblasts. Oncogene 2005; 24: 7257–7265.
Sokolov MV, Dickey JS, Bonner WM, Sedelnikova OA . gamma-H2AX in bystander cells—not just a radiation-triggered event, a cellular response to stress mediated by intercellular communication. Cell Cycle 2007; 6: 2210–2212.
Smilenov LB, Hall EJ, Bonner WM, Sedelnikova OA . A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts. Radiat Prot Dosimetry 2006; 122: 256–259.
Hu B, Wu L, Han W, Zhang L, Chen S, Xu A et al. The time and spatial effects of bystander response in mammalian cells induced by low dose radiation. Carcinogenesis 2006; 27: 245–251.
Han W, Wu L, Chen S, Bao L, Zhang L, Jiang E et al. Constitutive nitric oxide acting as a possible intercellular signaling molecule in the initiation of radiation-induced DNA double strand breaks in non-irradiated bystander cells. Oncogene 2006; 26: 2330–2339.
Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M et al. UV-induced skin damage. Toxicology 2003; 189: 21–39.
Halicka H, Huang X, Traganos F, King M, Dai W, Darzynkiewicz Z . Histone H2AX phosphorylation after cell irradiation with UV-B: relationship to cell cycle phase and induction of apoptosis. Cell Cycle 2005; 4: 339–345.
Tanaka T, Halicka H, Huang X, Traganos F, Darzynkiewicz Z . Constitutive histone H2AX phosphorylation and ATM activation, the reporters of DNA damage by endogenous oxidants. Cell Cycle 2006; 5: 1940–1945.
Ward IM, Chen J . Histone H2AX Is Phosphorylated in an ATR-dependent Manner in Response to Replicational Stress. J Biol Chem 2001; 276: 47759–47762.
Cuadrado M, Martinez-Pastor B, Fernandez-Capetillo O . ATR activation in response to ionizing radiation: still ATM territory. Cell Div 2006; 1: 7.
Mena S, Ortega A, Estrela J . Oxidative stress in environmental-induced carcinogenesis. Mutat Res 2009; 674: 36–44.
Pabla N, Huang S, Mi Q-S, Daniel R, Dong Z . ATR-Chk2 Signaling in p53 Activation and DNA damage response during cisplatin-induced apoptosis. J Biol Chem 2008; 283: 6572–6583.
Clingen PH, Wu JYH, Miller J, Mistry N, Chin F, Wynne P et al. Histone H2AX phosphorylation as a molecular pharmacological marker for DNA interstrand crosslink cancer chemotherapy. Biochem Pharmacol 2008; 76: 19–27.
Olive PL, Banath JP . Kinetics of H2AX phosphorylation after exposure to cisplatin. Cytometry B Clin Cytom 2009; 76B: 79–90.
Olive PL, Banath JP, Sinnott LT . Phosphorylated histone H2AX in spheroids, tumors, and tissues of mice exposed to etoposide and 3-amino-1,2,4-benzotriazine-1,3-dioxide. Cancer Res 2004; 64: 5363–5369.
Tanaka T, Huang X, Halicka HD, Zhao H, Traganos F, Albino A et al. Cytometry of ATM activation and histone H2AX phosphoryation to estimate extent of DNA damage induced by exogenous agents. Cytometry Part A 2007; 71A: 648–661.
Del Bino G, Lassota P, Darzynkiewicz Z . The S-phase cytotoxicity of camptothecin. Exp Cell Res 1991; 193: 27–35.
Banath JP, Olive PL . Expression of phosphorylated histone H2AX as a surrogate of cell killing by drugs that create DNA double-strand breaks. Cancer Res 2003; 63: 4347–4350.
Harikrishnan KN, Karagiannis TC, Chow MZ, El-Osta A . Effect of valproic acid on radiation-induced DNA damage in euchromatic and heterochromatic compartments. Cell Cycle 2008; 7: 468–476.
Karagiannis TC, Harikrishnan KN, El-Osta A . The histone deacetylase inhibitor, Trichostatin A, enhances radiation sensitivity and accumulation of gamma H2A.X. Cancer Biol Ther 2005; 4: 787–793.
Chinnaiyan P, Cerna D, Burgan WE, Beam K, Williams ES, Camphausen K et al. Postradiation sensitization of the histone deacetylase inhibitor valproic acid. Clin Cancer Res 2008; 14: 5410–5415.
Marks PA, Breslow R . Dimethyl sulfoxide to vorinostat: development of this histone deacetylase inhibitor as an anticancer drug. Nat Biotech 2007; 25: 84–90.
Bolden JE, Peart MJ, Johnstone RW . Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Discov 2006; 5: 769–784.
Xu WS, Parmigiani RB, Marks PA . Histone deacetylase inhibitors: molecular mechanisms of action. Oncogene 2007; 26: 5541–5552.
Chen C-S, Wang Y-C, Yang H-C, Huang P-H, Kulp SK, Yang C-C et al. Histone deacetylase inhibitors sensitize prostate cancer cells to agents that produce DNA double-strand breaks by targeting Ku70 acetylation. Cancer Res 2007; 67: 5318–5327.
Sanchez-Gonzalez B, Yang H, Bueso-Ramos C, Hoshino K, Quintas-Cardama A, Richon VM et al. Antileukemia activity of the combination of an anthracycline with a histone deacetylase inhibitor. Blood 2006; 108: 1174–1182.
Zuco V, Benedetti V, De Cesare M, Zunino F . Sensitization of ovarian carcinoma cells to the atypical retinoid ST1926 by the histone deacetylase inhibitor, RC307: enhanced DNA damage response. Int J Cancer 2009; 126: 1246–1255.
Fandy TE, Herman JG, Kerns P, Jiemjit A, Sugar EA, Choi S-H . et al. Early epigenetic changes and DNA damage do not predict clinical response in an overlapping schedule of 5-azacytidine and entinostat in patients with myeloid malignancies. Blood 2009; 114: 2764–2773.
Albino A, Jorgensen E, Rainey P, Gillman G, Clark T, Gietl D et al. gammaH2AX: a potential DNA damage response biomarker for assessing toxicological risk of tobacco products. Mutat Res 2009; 678: 43–52.