Évaluation de la plate-forme de PCR en temps réel LightCycler® 480 pour la mesure des charges virales CMV, EBV, HHV-6 et BKV dans le sang total

Razonable, 2003, Herpesvirus infections in transplant recipients: current challenges in the clinical management of cytomegalovirus and Epstein-Barr virus infections, Herpes, 10, 60 De Bolle, 2005, Update on human herpesvirus 6 biology, clinical features, and therapy, Clin Microbiol Rev, 18, 217, 10.1128/CMR.18.1.217-245.2005 Randhawa, 2006, BK virus infection in transplant recipients: An overview and update, Am J Transplant, 6, 2000, 10.1111/j.1600-6143.2006.01403.x Deback, 2007, Monitoring of human cytomegalovirus infection in immunosuppressed patients using real-time PCR on whole blood, J Clin Virol, 40, 173, 10.1016/j.jcv.2007.08.014 Gautheret-Dejean, 2002, Development of a real-time PCR assay for the diagnosis of human herpesvirus-6 infection and application to bone marrow transplant recipients, J Virol Methods, 100, 27, 10.1016/S0166-0934(01)00390-1 Si-Mohamed, 2006, Detection and quantitation of BK virus DNA by real-time polymerase chain reaction in the LT-ag gene in adult renal transplant recipients, J Virol Methods, 131, 21, 10.1016/j.jviromet.2005.06.025 Brengel-Pesce, 2002, Routine use of real-time quantitative PCR for laboratory diagnosis of Epstein-Barr virus infections, J Med Virol, 66, 360, 10.1002/jmv.2153 Dumonceaux, 2008, Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK, J Clin Microbiol, 46, 2829, 10.1128/JCM.00844-08 Molenkamp, 2007, Simultaneous detection of five DNA targets by real-time TaqMan PCR using the Roche LightCycler® 480: Application in viral molecular diagnostics, J Virol Methods, 141, 205, 10.1016/j.jviromet.2006.12.007 Stevens, 2008, Development and validation of a real-time PCR assay specifically detecting human papillomavirus 52 using the Roche LightCycler® 480 system, J Virol Methods, 147, 290, 10.1016/j.jviromet.2007.09.018 Boutolleau, 2003, Human herpesvirus (HHV)-6 and HHV-7: two closely related viruses with different infection profiles in stem cell transplant recipients, J Infect Dis, 187, 179, 10.1086/367677 Fafi-Kremer, 2004, Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein-Barr virus load in whole blood, peripheral mononuclear cells and plasma, J Clin Virol, 30, 157, 10.1016/j.jcv.2003.10.002 Yeo, 2008, The prevalence of BK polyomavirus infection in outpatient kidney transplant recipients followed in a single center, Clin Transplant, 22, 532, 10.1111/j.1399-0012.2008.00817.x