Khoa Sinh học, Trường Đại học Khoa học Tự nhiên - Đại học Quốc gia Hà Nội

Objective: to examine genetic and functional stability of an osteoblast reporter transgene coding for green fluorescence protein (GFP) in the transgenic col10a1:nlGFP fish generated a decade ago. Methods: homozygous and hemizygous fish for the transgene GFP were segregated by testcrossing. PCR were performed to check for the presence of the transgene GFP in the homozygous and hemizygous genomes.  GFP signal was used to assess distribution of collagen10a1 expressing osteoblasts. Alizarin complexone (ALC) was used to visualize mineralized matrix of the live larvae. Expression pattern of the transgene GFP and level of bone mineralization in the live transgenic fish was analyzed using fluorescent imaging and ImageJ analysis for GFP and ALC signal, respectively. Results: three homozygous col10a1:nlGFP fish were found and many hemizygotes were produced. Both homozygous and hemizygous fish still contain the transgene GFP in their genomes and express GFP in a pattern recapitulating endogenous collagen10a1 gene expression in osteoblast. They also retain the pattern of GFP expression in bone structures like that of the original transgenic fish generated a decade ago. This confirms the genomic and functional stability of the transgene GFP in the fish

Objective: This study aimed to evaluate the protective effects of Vietnamese ginseng extract(VGE) on the formation of reactive oxygen species (ROS) in Cobalt chloride (CoCl2)-exposedH9C2 cells. Methods: H9C2 cells were subjected to CoCl -induced hypoxia-reoxygenation (HR)model with or without treatment of VGE. The hydroperoxide (H2O2) and superoxide (O2-)productions in H9C2 cells in the experimental groups were assessed by suitable fluorescencekits. Results: The obtained data showed that VGE at a dose of 31.25 μg/ml significantly reducedROS levels in HR-subjected H9C2 cells. In the HR group, the H2O2 and O2- content in H9C2 cellswere sharply increased to 156.81±7.82% and 160.10±2.07% (of 100% control), respectively.Interestingly, in the VGE-treated HR group, these indicators were significantly decreased to119.67± 3.37% and 124.72 ± 3.21% (p <0.05), respectively. Conclusion: The resultsdemonstrated that VGE effectively attenuated oxidative stress in cardiomyocytes under CoCl2-induced HR condition.

Melanin từ dịch túi mực ở loài mực biển được chứng minh là một chất chống oxy hóa mạnh, trong nghiên cứu này, chúng tôi tiến hành khảo sát tác dụng của melanin tách chiết từ dịch túi mực trong việc điều trị viêm gan trên đối tượng chuột Swiss được gây viêm gan mạn tính bằng tetrachloro carbon (CCl4). Kết quả nghiên cứu cho thấy, điều trị bằng melanin làm giảm nồng độ malondialdehyde (MDA) trong gan và tín hiệu phát quang hóa học phụ thuộc luminol của máu tổng số (tăng cao trong điều kiện viêm) về mức độ bình thường. Kết quả phân tích mô học cũng cho thấy điều trị bằng melanin giúp phục hồi các tế bào gan bị tổn thương một cách hiệu quả. Hơn nữa, phân tích biểu hiện gene cho thấy melanin làm tăng mức độ biểu hiện của các gen superoxide dismutase (SOD), glutathione peroxidase (GP-x) catalse (CAT) và làm giảm mức độ biểu hiện của gen cyclooxygenase-2 (COX-2) ở trên mẫu mô gan của chuột được điều trị so với trên mẫu mô gan của chuột đối chứng hoặc chuột không được điều trị có ý nghĩa thống kê. Kết quả nghiên cứu chỉ ra rằng melanin có tác dụng tốt trong việc chống viêm và có tiềm năng ứng dụng làm chất bổ sung trong thực phẩm, hỗ trợ điều trị stress oxy hóa hoặc viêm gan.

Noi chicken is one of the indigenous fighting-cock breeds of Vietnam with many valuable properties. In chicken, the Golgin subfamily B member 1 (GOLGB1) gene, which locates on chromosome 1, encodes the coat protein 1 vesicle inhibiting factor. A previous study showed that the 65-bp insertion/deletion in the GOLGB1 gene was significantly associated with chicken body weight, neck weight, abdominal fat weight, abdominal fat percentage and the yellow index b of the breast in Chinese indigenous chicken (N409-breed). In this study, 65-bp indel polymorphism in the GOLGB1gene was evaluated in association with body weight in Noi chickens. The live body weight of 170 chickens (90 females, 80 males) at continuous ages of 7-day intervals (from 28 to 84 days) was recorded. A 65-bp indel polymorphism in the GOLGB1 gene was analyzed using the PCR method. The results of PCR and sequencing revealed two alleles (I and D) corresponding to three genotypes: II, ID, DD, in which, the I allele appears with the highest frequency (0.79) in the studied population. An association study using Minitab software showed that genotype was significantly associated with body weight in Noi chickens at days 35 to 84 (P < 0.05). Individuals with more allele “I” have higher live body weight than others. Both factors (sex and genotype) simultaneously affected the body weight of Noi chickens. The results of this study suggested that the 65-bp indel in the GOLGB1 gene could be considered a potential marker for Noi chicken breeding.

Over the recent years, results from investigation into the family Horseshoe bats (Rhinolophidae) indicated that the taxonomic status of many species in Vietnam is still unclear. Having studied on 1,140 specimens of the genus Rhinolophus, we confirm that Vietnam is a home to 20 horseshoe bat species. Remarkably, our study results obtained 6 unusual forms belonging to 5 species complexes: R. affinis, R. malayanus, R. thomasi, R. paradoxolophus and R. marshalli. These froms differ from the described species in all morphology, echolocation and genetics. Conservation status of 4 rarely documented species of horseshoe bats from Vietnam: R. thomasi, R. paradoxolophus, R. osgoodi and R. luctus is also given in this paper.   Citation: Hoang Trung Thanh, Nguyen Truong Son, Nguyen Van Sang, Dinh Nho Thai, Nguyen Huy Hoang, Vuong Tan Tu, Nguyen Xuan Huan, Vu Dinh Thong, 2017. Diversity, taxonomy and conservation status of horseshoe bats (Chiroptera: Rhinolophidae) in Vietnam. Tap chi Sinh hoc, 39(2): 174-184. DOI: 10.15625/0866-7160/v39n2.9213 *Corresponding author: [email protected] Received 11 February 2017, accepted 16 February 2017