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other provinces and regions in Vietnam and other country.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Address\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Headquarters of Can Tho Journal of Medicine and Pharmacy, located Scientific Research and International Cooperation Office: 179 Nguyen Van Cu Street, An Khanh Ward, Ninh Kieu District, Can Tho City, Vietnam.\u003C\u002Fspan>\u003C\u002Fp>","\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Ngày 16\u002F7\u002F2015, Tạp chí Y Dược học Cần Thơ được cấp chỉ số quốc tế: ISSN 2354-1210.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Từ tháng 4\u002F2016, Tạp chí đã được Hội đồng Giáo sư ngành Y đưa vào danh sách các tạp chí khoa học Y học được tính điểm công trình 0-0,5 điểm cho một bài báo đăng.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Năm 2020 Tạp chí Y Dược học Cần Thơ đã được phê duyệt vào danh mục của các Hội đồng Giáo sư ngành Dược học được tính điểm công trình 0-0,5 điểm cho một bài báo đăng.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tạp chí Y Dược học Cần Thơ ra 12 số\u002Fnăm, 180-200 trang\u002Fsố.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Từ tháng 12\u002F2022 Tạp chí Y Dược học Cần Thơ là thành viên của hệ thống Crossref và từ tháng 01\u002F2023 tạp chí thực hiện bình duyệt online kín 2 chiều nhằm tăng tính minh bạch, tin cậy của các công trình nghiên cứu khoa học và đảm bảo tốt nhất chất lượng khoa học của bài viết.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tôn chỉ, mục đích và phạm vi của tạp chí\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tôn chỉ và mục đích hoạt động của tạp chí: xuất bản nhằm mục đích phổ biến kết quả từ các đề tài nghiên cứu khoa học; giao lưu trao đổi khoa học, chia sẻ kinh nghiệm, học tập, đồng thời cập nhật thông tin khoa học mới trong các lĩnh vực y, sinh, dược học trong và ngoài nước.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Phạm vi của tạp chí: Tạp chí xuất bản được chia thành 3 chuyên mục: (i) Bài báo nghiên cứu khoa học là kết quả công trình nghiên cứu khoa học có giá trị đã được triển khai nghiên cứu, (ii) Bài tổng quan y, sinh, dược học: phục vụ mục tiêu đào tạo liên tục trong lĩnh vực y, sinh, dược học; nhằm hệ thống hóa những kiến thức kinh điển và hiện đại; (iii) Thông tin cập nhật kiến thức mới về y, sinh, dược học trong nước và trên thế giới.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Chính sách truy cập mở\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tạp chí Y Dược học Cần Thơ áp dụng chính sách truy cập mở đối với các bài báo đã xuất bản đến với độc giả, nhằm mở rộng cơ hội tiếp cận các kết quả nghiên cứu chất lượng cao và tăng cường trao đổi kiến thức. Tạp chí đăng tải trực tuyến (miễn phí) toàn văn các bài báo được công bố trên website của Tạp chí (https:\u002F\u002Ftapchi.ctump.edu.vn).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Đạo đức xuất bản\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tạp chí Y Dược học Cần Thơ cam kết tuân thủ đạo đức xuất bản phù hợp với các hướng dẫn và tiêu chuẩn của the Committee on Publication Ethics (COPE), tuân thủ các nguyên tắc của COPE’s Core Practices, Best Practices Guidelines for Journal Editors và Guidelines on Good Publication Practices.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Bản thảo bài báo chỉ được chấp nhận khi được tác giả chịu trách nhiệm chính cam kết các nội dung sau: Các nội dung của bản thảo chưa được đăng tải toàn bộ hoặc một phần ở các tạp chí khác; Tất cả các tác giả đều có đóng góp một cách đáng kể vào quá trình nghiên cứu hoặc chuẩn bị bản thảo và cùng chịu trách nhiệm về các nội dung của bản thảo; Tuân thủ các biện pháp đảm bảo đạo đức nghiên cứu (ví dụ thỏa thuận đồng ý tham gia nghiên cứu).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Cam kết bảo mật\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tạp chí cam kết thực hiện và tuân thủ các quy định của luật và các văn bản hướng dẫn liên quan đến bảo mật thông tin cá nhân trên không gian mạng. Các thông tin mà người dùng (tác giả, độc giả, biên tập viên, người phản biện) nhập vào các biểu mẫu trên Hệ thống Quản lý xuất bản trực tuyến của tạp chí chỉ được sử dụng vào các mục đích đã được tuyên bố rõ ràng và sẽ không được cung cấp cho bất kỳ bên thứ ba nào khác, hay dùng vào bất kỳ mục đích nào khác.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Phí gửi bài\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Lệ phí gửi đăng bài: 1.000.000đ\u002Fbài báo\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Lệ phí gửi đăng nhanh: 1.500.000đ\u002Fbài báo\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Đối với tác giả là cán bộ viên chức thuộc Trường Đại học Y Dược Cần Thơ thì được hỗ trợ 50% lệ phí gửi đăng bài.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Đối với sinh viên thực hiện đề tài nghiên cứu khoa học cấp trường được hỗ trợ 100% lệ phí đăng bài ( Tác giả gửi đính kèm “ Quyết định về việc giao tổ chức thực hiện đề tài nghiên cứu khoa học cấp Trường của sinh viên”).\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Hình thức nộp lệ phí:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">1. Tiền mặt:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Nộp trực tiếp tại Phòng Tài chính - Kế toán, Trường Đại học Y Dược Cần Thơ, số 179 Nguyễn Văn Cừ, P. An Khánh, Q. Ninh Kiều, thành phố Cần Thơ.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">2. Chuyển khoản:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tên Tài khoản: Trường ĐHYD Cần Thơ, Số TK: 0111000115668, tại ngân hàng Vietcombank chi nhánh Cần Thơ.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Thời gian: Áp dụng từ ngày 01\u002F02\u002F2023.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">* Phí gửi bài không được hoàn trả khi bài viết bị từ chối hoặc tác giả xin rút bài viết.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Quy trình phản biện bài báo\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tạp chí Y Dược học Cần Thơ thực hiện quy trình phản biện kín hai chiều nghiêm ngặt. Danh tính của những người phản biện không được tiết lộ cho các tác giả và ngược lại. Quy trình thẩm định bài báo đăng gồm các bước sau:\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tiếp nhận bản thảo\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Tác giả liên hệ gửi bản thảo đến Tạp chí qua hệ thống trực tuyến tại website: https:\u002F\u002Ftapchi.ctump.edu.vn. Hướng dẫn về cách đăng ký, gửi bài và chuẩn bị bản thảo được cung cấp trên website của Tạp chí.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Sàng lọc sơ bộ\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Sau khi Tòa soạn nhận được bài báo của tác giả, Ban Thư ký sẽ tiến hành kiểm tra sơ bộ bài báo (các yêu cầu về nội dung và hình thức). Những bài báo không đúng quy cách hoặc có nội dung không phù hợp hoặc vi phạm bản quyền sẽ bị từ chối (Ban Thư ký thông báo phản hồi đến tác giả trong vòng 1 tuần). Những bài báo đủ điều kiện, được Ban Thư ký tòa soạn chuyển đến Ban Biên tập có cùng chuyên môn với nội dung bài báo để đề xuất người phản biện. Thời gian kể từ khi Ban Biên tập nhận bài báo đến khi đề xuất người phản biện bài báo chậm nhất là 5 ngày.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Vòng phản biện\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">1. Ban Thư ký gửi bài và yêu cầu phản biện đến 02 phản biện độc lập.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">2. Các phản biện gởi nhận xét cho Ban Thư ký. Thời gian từ khi gửi bài cho phản biện đến khi nhận ý kiến của phản biện tối đa là 20 ngày.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Xử ký kết quả phản biện\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">1. Nếu ý kiến đồng ý cho đăng và không cần chỉnh sửa, Ban Thư ký tiếp tục đăng bài theo qui trình.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">2. Nếu ý kiến đồng ý đăng và cần chỉnh sửa, Ban Thư ký sẽ thông tin đến tác giả chỉnh sửa theo yêu cầu của người phản biện. Thời gian chỉnh sửa và gửi lại kéo dài không quá 2 tuần, từ khi tác giả bài báo nhận được thông tin (Quá trình này có thể lặp lại tối đa 2 lần\u002F1 bài báo). Khi có sự thống nhất, đồng ý của người phản biện; bài báo được tiếp tục đăng theo qui trình.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">3. Những bài báo có chất lượng không đạt yêu cầu, cả 2 phản biện không đồng ý cho đăng sẽ bị Tòa soạn từ chối đăng.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">Xuất bản\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">1. Ban Thư ký tổng hợp các bản thảo đã được tác giả hoàn thiện sau thẩm định trình Ban Biên tập xem xét, Tổng Biên tập phê duyệt, quyết định bài đăng theo các tiêu chí: sự phù hợp nội dung với tôn chỉ và mục đích, thể loại bài viết (ưu tiên các bài có bài có nghiên cứu chuyên sâu, hàm lượng khoa học cao), đóng góp mới bài báo, bài báo được ưu tiên đăng trong số gần nhất của Tạp chí theo thứ tự: tính thời sự, chất lượng bài báo và thời gian gửi bài.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">2. Ban Biên tập và Ban Thư ký biên tập bản thảo, chế bản, đọc rà soát lỗi. Thời gian hoàn thành từ 10-15 ngày.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">3. Ban Thư ký có trách nhiệm thông báo cho tác giả bài báo (bằng e-mail) về tình hình phê duyệt bài báo, thời gian, số kỳ, tập xuất bản bài báo theo qui định.\u003C\u002Fspan>\u003C\u002Fp>\u003Cp>\u003Cbr>\u003C\u002Fp>\u003Cp>\u003Cspan style=\"color: rgb(0, 0, 0);\">4. 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The individual rate of development of gingivitis was closely correlated with the rate of plaque accumulation. Characteristic bacteriological changes were revealed in the plaque along the gingival margin during this experiment. Initially, i.e. when the teeth were clean and the gingiva healthy, the extremely sparse plaque flora consisted almost exclusively of gram‐positive cocci and rods. The first phase of plaque development occurred during the first 2 days without oral hygiene and consisted of a proliferation of the gram‐positive cocci and rods and an addition of about 30 per cent gram‐negative cocci and rods. During the second phase (after 1–4 days) fusobacteria and filaments appeared and increased until they each made up about seven per cent of the flora. During the third phase (after 4–9 days) the flora was supplemented with spirilla and spirochetes, and at the end of the period without oral hygiene each of these two groups of organisms accounted for about two per cent of the plaque flora. In specific areas the gingival condition was correlated with the composition of the plaque and it was found that mild gingivitis could be diagnosed clinically at approximately the same time as the complex flora was established. However, sub‐clinical inflammation started much earlier, probably as a reaction to the first phases of plaque development. When oral hygiene was reinstituted, the plaque in most areas disappeared in 1–2 days and after 7–11 days the Plaque Index for each subject was as low as before the experiment. Correspondingly, after 1–2 days most tooth surfaces only harbored the original sparse flora of gram‐positive cocci and rods. The gingival inflammation in an area usually disappeared one day after the plaque had been removed.\u003C\u002Fjats:p>",{"EN":762},"Experimental gingivitis in man",{"VOID":764},"4224181",{"VOID":766},"10.1111\u002Fj.1600-0765.1966.tb01842.x","PUBLICATION","Auto Verify",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.1966.tb01842.x",[772,791,806,821],{"id":773,"sortIndex":115,"researcher":26,"roles":774,"affiliations":775,"properties":786},"a172a7cf-7d91-4f33-aa3f-5c3dec9dcdb0",[],[776],{"id":777,"sortIndex":36,"affiliation":778,"properties":26},"4e265dee-79d4-444a-b499-c30f214eb8c9",{"id":779,"createTime":780,"updateTime":780,"relativeEntities":781,"slug":782,"properties":783,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"36d8492f-3c31-4460-834e-a23b276e12e0","2025-01-25T22:20:35.486+00:00",[],"The-Departments-of-Microbiology-Oral-Diagnosis-and-Periodontology-The-Royal-Dental-College-Aarhus-Denmark",{"title":784},{"EN":785},"The Departments of Microbiology, Oral Diagnosis, and Periodontology, The Royal Dental College, Aarhus, Denmark",{"openalex":787,"title":789},{"VOID":788},"A5028573509",{"EN":790},"W. 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Ref, 146, 189",{},{"id":26,"text":894,"url":26,"identifiers":895},"Löe H., 1965, Absence and presence of fluid from normal and inflamed gingivae, Periodontics, 3, 171",{},{"id":26,"text":897,"url":26,"identifiers":898},"10.3109\u002F00016356309011240",{"doi":897},{"id":26,"text":900,"url":26,"identifiers":901},"10.1902\u002Fjop.1965.36.3.177",{"doi":900},{"id":26,"text":903,"url":26,"identifiers":904},"10.1177\u002F00220345630420016301",{"doi":903},{"id":26,"text":906,"url":26,"identifiers":907},"10.1093\u002Finfdis\u002F108.3.304",{"doi":906},{"id":26,"text":909,"url":26,"identifiers":910},"10.1177\u002F00220345560350030401",{"doi":909},{"id":26,"text":912,"url":26,"identifiers":913},"10.1177\u002F00220345540330040301",{"doi":912},{"id":26,"text":915,"url":26,"identifiers":916},"10.1177\u002F00220345550340061901",{"doi":915},{"id":26,"text":918,"url":26,"identifiers":919},"10.1177\u002F00220345560350022301",{"doi":918},{"id":26,"text":921,"url":26,"identifiers":922},"10.3109\u002F00016356408993968",{"doi":921},{"id":26,"text":924,"url":26,"identifiers":925},"10.1093\u002Finfdis\u002F115.3.247",{"doi":924},{"id":26,"text":927,"url":26,"identifiers":928},"10.1016\u002F0003-9969(63)90019-0",{"doi":927},{"id":26,"text":930,"url":26,"identifiers":931},"10.1902\u002Fjop.1964.35.3.197",{"doi":930},false,{"id":934,"createTime":935,"updateTime":935,"relativeEntities":936,"slug":937,"properties":938,"entityType":767,"verifyStatus":25,"verifyTime":935,"verifyNote":768,"syncStatus":28,"languages":952,"translateLanguages":26,"viewCount":36,"primaryUrl":953,"fullTextUrl":26,"authors":954,"publicationType":836,"publisherRelationship":1078,"citationCount":1111,"citationInfo":1112,"publishDate":1114,"publishYear":1115,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":1116,"isForceReanalyzing":932},"ab1aa165-de57-4210-aaec-44faa4cdee65","2024-09-28T15:01:02.429+00:00",[],"Susceptibility-of-various-oral-bacteria-to-antimicrobial-peptides-and-to-phagocytosis-by-neutrophils",{"mag":939,"keywords":941,"openalex":942,"abstract":944,"title":946,"pm":948,"doi":950},{"VOID":940},"2027183942",{},{"VOID":943},"W2027183942",{"EN":945},"\u003Cjats:p>\u003Cjats:bold>Background and Objective: \u003C\u002Fjats:bold> The aim of this study was to compare the susceptibility of nonperiodontopathic and periodontopathic bacteria to major defense mechanisms for bacterial clearance in gingival sulcus.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Material and Methods: \u003C\u002Fjats:bold> Twenty strains of 13 oral bacterial species were studied for their susceptibility to phagocytosis by human neutrophils and to the antimicrobial peptides LL‐37 and human beta defensin‐3. The minimum inhibitory concentrations of LL‐37 and human beta defensin‐3 were determined by a liquid dilution assay, and susceptibility to phagocytosis was examined by a flow cytometric phagocytosis assay.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results: \u003C\u002Fjats:bold> The minimum inhibitory concentrations of LL‐37 and human beta defensin‐3 varied greatly, depending on the strain and species. Although a significant difference between the non‐ and periodontopathic groups was not observed, the red‐complex bacteria were more resistant to LL‐37 than the others (\u003Cjats:italic>p =\u003C\u002Fjats:italic> 0.004). The susceptibility of oral bacteria to phagocytosis was quite variable, depending on the species but not on the strains\u003Cjats:italic>.\u003C\u002Fjats:italic> The periodontopathic bacteria, especially \u003Cjats:italic>Actinobacillus actinomycetemcomitans\u003C\u002Fjats:italic> and the red‐complex triad, were more resistant to phagocytosis than were the nonperiodontopathic bacteria (\u003Cjats:italic>p\u003C\u002Fjats:italic> = 0.0003). In addition, bacteria resistant both to antimicrobial peptides and to phagocytosis were more common in the periodontopathic group.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusion: \u003C\u002Fjats:bold> Our results indicate that immune evasion may contribute to the pathogenicity of some periodontopathic bacteria.\u003C\u002Fjats:p>",{"EN":947},"Susceptibility of various oral bacteria to antimicrobial peptides and to phagocytosis by neutrophils",{"VOID":949},"17760818",{"VOID":951},"10.1111\u002Fj.1600-0765.2006.00962.x",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.2006.00962.x",[955,974,1006,1023,1044,1061],{"id":956,"sortIndex":114,"researcher":26,"roles":957,"affiliations":958,"properties":969},"0e572732-e625-46b5-85fd-afcaf730f6cf",[],[959],{"id":960,"sortIndex":36,"affiliation":961,"properties":26},"5ab2028b-df23-43cb-8613-5821c41124dd",{"id":962,"createTime":963,"updateTime":963,"relativeEntities":964,"slug":965,"properties":966,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"fe87697b-b30b-4f47-bbfc-c8dcc1d5dab0","2024-09-28T15:01:02.466+00:00",[],"Department-of-Statistics-College-of-Natural-Sciences-Chonnam-National-University-Gwangju-Korea",{"title":967},{"EN":968},"Department of Statistics, College of Natural Sciences, Chonnam National University, Gwangju, Korea",{"openalex":970,"title":972},{"VOID":971},"A5008857923",{"EN":973},"E. 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S, 2005, Measurement of bacterial (E. coli) concentration by flow cytometry, Int J Oral Biol, 30, 65",{},{"id":26,"text":1187,"url":26,"identifiers":1188},"10.1074\u002Fjbc.274.1.29",{"doi":1187},{"id":26,"text":1190,"url":26,"identifiers":1191},"Clark RA, 1996, Current Protocols in Immunology, 7.23.1",{},{"id":26,"text":1193,"url":26,"identifiers":1194},"10.1128\u002FAAC.47.7.2339-2341.2003",{"doi":1193},{"id":26,"text":1196,"url":26,"identifiers":1197},"10.1111\u002Fj.1600-051X.1998.tb02419.x",{"doi":1196},{"id":26,"text":1199,"url":26,"identifiers":1200},"10.1111\u002Fj.1601-0825.1995.tb00174.x",{"doi":1199},{"id":26,"text":1202,"url":26,"identifiers":1203},"10.1128\u002FJCM.37.12.4028-4033.1999",{"doi":1202},{"id":26,"text":1205,"url":26,"identifiers":1206},"Kolodrubetz D, 1996, cis Elements and trans factors are both important in strain‐specific regulation of the leukotoxin gene in Actinobacillus actinomycetemcomitans, Infect Immun, 64, 3451, 10.1128\u002Fiai.64.9.3451-3460.1996",{"doi":1207},"10.1128\u002Fiai.64.9.3451-3460.1996",{"id":26,"text":1209,"url":26,"identifiers":1210},"10.1016\u002FS0022-1759(00)00237-4",{"doi":1209},{"id":26,"text":1212,"url":26,"identifiers":1213},"10.1126\u002Fscience.1096158",{"doi":1212},{"id":26,"text":1215,"url":26,"identifiers":1216},"10.1007\u002FBF01641619",{"doi":1215},{"id":26,"text":1218,"url":26,"identifiers":1219},"10.1111\u002Fj.1462-5822.2005.00601.x",{"doi":1218},{"id":26,"text":1221,"url":26,"identifiers":1222},"10.1016\u002Fj.immuni.2005.05.002",{"doi":1221},{"id":26,"text":1224,"url":26,"identifiers":1225},"10.1182\u002Fblood-2003-04-1078",{"doi":1224},{"id":26,"text":1227,"url":26,"identifiers":1228},"10.1016\u002Fj.archoralbio.2004.07.002",{"doi":1227},{"id":26,"text":1230,"url":26,"identifiers":1231},"10.1111\u002Fj.1600-051X.1993.tb01752.x",{"doi":1230},{"id":26,"text":1233,"url":26,"identifiers":1234},"10.1902\u002Fjop.1980.51.2.70",{"doi":1233},{"id":26,"text":1236,"url":26,"identifiers":1237},"Storch H, 1989, Selective IgA defect. Clinical importance for stomatology, Stomatol DDR, 39, 771",{},{"id":1239,"createTime":1240,"updateTime":1240,"relativeEntities":1241,"slug":1242,"properties":1243,"entityType":767,"verifyStatus":25,"verifyTime":1240,"verifyNote":768,"syncStatus":28,"languages":1257,"translateLanguages":26,"viewCount":36,"primaryUrl":1258,"fullTextUrl":26,"authors":1259,"publicationType":836,"publisherRelationship":1280,"citationCount":1313,"citationInfo":1314,"publishDate":1316,"publishYear":1317,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":1318,"isForceReanalyzing":932},"1696690b-02c4-4e5e-b8cc-149c9b383c31","2025-02-09T19:38:03.286+00:00",[],"Inflammatory-mediators-and-the-destruction-of-bone",{"mag":1244,"keywords":1246,"openalex":1247,"abstract":1249,"title":1251,"pm":1253,"doi":1255},{"VOID":1245},"1965740308",{},{"VOID":1248},"W1965740308",{"EN":1250},"\u003Cjats:p>Bone is remodelled by the coordinated actions of osteoclasts and osteoblasts. Cellular remodelling occurs in discrete packets of bone, and is regulated by local cytokines produced in the environment of the remodelling cells. These cytokines are secreted by immune cells and by bone cells. In addition, some growth regulatory factors are incorporated into the noncollagenous bone matrix and are released in an active form when bone is stimulated to resorb. Complex interactions between these cytokines and their target cells are responsible for the normal delicate balance between bone resorption and bone formation, and disorders of bone loss are due to imbalances between the rates of resorption and formation.\u003C\u002Fjats:p>",{"EN":1252},"Inflammatory mediators and the destruction of bone",{"VOID":1254},"1831844",{"VOID":1256},"10.1111\u002Fj.1600-0765.1991.tb01647.x",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.1991.tb01647.x",[1260],{"id":1261,"sortIndex":36,"researcher":26,"roles":1262,"affiliations":1263,"properties":1275},"d9455f00-67f0-4079-bcb6-8e9378fc1f45",[],[1264],{"id":1265,"sortIndex":36,"affiliation":1266,"properties":26},"700e8623-1ded-4234-b289-42ddd5335cc3",{"id":1267,"createTime":1268,"updateTime":1269,"relativeEntities":1270,"slug":1271,"properties":1272,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"afb8376a-6aaf-4d11-b6aa-7ff7d7f39137","2024-01-01T10:52:48.633+00:00","2025-02-09T19:38:03.300+00:00",[],"University-of-Texas-Health-Science-Center-San-Antonio-Texas-USA",{"title":1273},{"VI":1274},"University of Texas Health Science Center San Antonio, Texas USA",{"openalex":1276,"title":1278},{"VOID":1277},"A5110026446",{"EN":1279},"Gregory R. Mundy",{"url":26,"publisher":1281,"properties":1306},{"id":659,"createTime":660,"updateTime":661,"relativeEntities":1282,"slug":663,"properties":1283,"entityType":24,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36,"subjectFields":1289,"manageAffiliations":1290,"indexDatabases":1291,"url":743,"thumbnailPath":26,"statistic":26,"gsStatistic":26,"type":26,"analyzePriority":26},[],{"country":1284,"issn":1285,"introduce":1286,"eissn":1287,"title":1288},{"VOID":666},{"VOID":668},{"EN":670},{"VOID":672},{"EN":674},[],[],[1292,1299],{"id":725,"indexDatabase":1293,"url":740,"indexYears":26,"academicFieldIds":1298,"indexDatabaseRanking":26},{"id":727,"createTime":728,"updateTime":729,"relativeEntities":1294,"label":1295,"description":1296,"key":736,"publicationTags":1297,"standard":26},[],{"EN":732,"VI":732},{"VI":734,"EN":735},[738,739],[742],{"id":706,"indexDatabase":1300,"url":719,"indexYears":720,"academicFieldIds":1305,"indexDatabaseRanking":723},{"id":708,"createTime":709,"updateTime":710,"relativeEntities":1301,"label":1302,"description":1303,"key":716,"publicationTags":1304,"standard":26},[],{"EN":713,"VI":713},{"EN":713,"VI":715},[718],[722],{"volume":1307,"pages":1309,"issue":1311},{"VOID":1308},"26",{"VOID":1310},"213-217",{"VOID":1312},"3",114,{"total":1313,"publishYear":26,"statisticByYear":1315},{"2012":59,"2014":59,"2015":111,"2016":59,"2017":115,"2018":114,"2020":114,"2021":115,"2022":115,"2023":111},"1991-05-01",1991,[1319,1322,1325,1328,1331,1334,1337,1340,1343,1347,1350,1353,1356,1359,1362,1365,1369,1372,1375,1378,1381,1384,1387,1390,1393,1396,1399,1402,1405,1408],{"id":26,"text":1320,"url":26,"identifiers":1321},"10.1073\u002Fpnas.85.15.5683",{"doi":1320},{"id":26,"text":1323,"url":26,"identifiers":1324},"10.1172\u002FJCI113647",{"doi":1323},{"id":26,"text":1326,"url":26,"identifiers":1327},"10.1073\u002Fpnas.87.8.3023",{"doi":1326},{"id":26,"text":1329,"url":26,"identifiers":1330},"10.1210\u002Fendo-126-6-3069",{"doi":1329},{"id":26,"text":1332,"url":26,"identifiers":1333},"10.1016\u002F0006-291X(89)91143-1",{"doi":1332},{"id":26,"text":1335,"url":26,"identifiers":1336},"YatesAJ OreffoROC MayorK MundyGR.Inorganic phosphate inhibits osteoclast formation and the activity of mature osteoclasts.J Bone Min Res1990(in press).",{},{"id":26,"text":1338,"url":26,"identifiers":1339},"10.1016\u002FS0021-9258(18)67143-1",{"doi":1338},{"id":26,"text":1341,"url":26,"identifiers":1342},"10.1073\u002Fpnas.82.8.2267",{"doi":1341},{"id":26,"text":1344,"url":26,"identifiers":1345},"Seyedin SM, 1986, Cartilage‐inducing factor A: Apparent identity to transforming growth factor beta, J Biol Chem, 261, 5693, 10.1016\u002FS0021-9258(17)38436-3",{"doi":1346},"10.1016\u002FS0021-9258(17)38436-3",{"id":26,"text":1348,"url":26,"identifiers":1349},"10.1210\u002Fendo-124-6-2991",{"doi":1348},{"id":26,"text":1351,"url":26,"identifiers":1352},"Marcelli C, 1990, In vivo effects of human recombinant transforming growth factor beta on bone turnover in normal mice, Calcif Tiss Int, 46, A40",{},{"id":26,"text":1354,"url":26,"identifiers":1355},"10.1172\u002FJCI113318",{"doi":1354},{"id":26,"text":1357,"url":26,"identifiers":1358},"10.1210\u002Fendo-118-1-74",{"doi":1357},{"id":26,"text":1360,"url":26,"identifiers":1361},"10.1210\u002Fendo-125-3-1142",{"doi":1360},{"id":26,"text":1363,"url":26,"identifiers":1364},"10.1210\u002Fendo-125-5-2780",{"doi":1363},{"id":26,"text":1366,"url":26,"identifiers":1367},"Obberghen‐Schilling E, 1988, Transforming growth factor‐β positively regulates its own expression in normal and transformed cells, J Biol Chem, 263, 7741, 10.1016\u002FS0021-9258(18)68561-8",{"doi":1368},"10.1016\u002FS0021-9258(18)68561-8",{"id":26,"text":1370,"url":26,"identifiers":1371},"10.1126\u002Fscience.3969553",{"doi":1370},{"id":26,"text":1373,"url":26,"identifiers":1374},"10.1111\u002Fj.1600-051X.1989.tb02334.x",{"doi":1373},{"id":26,"text":1376,"url":26,"identifiers":1377},"10.1073\u002Fpnas.84.13.4616",{"doi":1376},{"id":26,"text":1379,"url":26,"identifiers":1380},"10.1073\u002Fpnas.86.7.2398",{"doi":1379},{"id":26,"text":1382,"url":26,"identifiers":1383},"10.1056\u002FNEJM198708273170902",{"doi":1382},{"id":26,"text":1385,"url":26,"identifiers":1386},"10.1182\u002Fblood.V73.8.2145.2145",{"doi":1385},{"id":26,"text":1388,"url":26,"identifiers":1389},"10.1182\u002Fblood.V74.1.380.380",{"doi":1388},{"id":26,"text":1391,"url":26,"identifiers":1392},"10.1172\u002FJCI114392",{"doi":1391},{"id":26,"text":1394,"url":26,"identifiers":1395},"BlackKS MundyGR GarrettIR.Interleukin‐6 causes hypercalcemia in vivo and enhances the bone resorbing potency of interleukin‐1 and tumor necrosis factor by two orders of magnitude in vitro.J Bone Min Res1990(in press).",{},{"id":26,"text":1397,"url":26,"identifiers":1398},"Sato K, 1989, Paraneoplastic syndrome of hypercalcemia and leukocytosis caused by squamous carcinoma cells (T3M‐1) producing parathyroid hormone‐related protein, interleukin‐1‐alpha, and granulocyte colony‐stimulating factor, Cancer Res, 49, 4740",{},{"id":26,"text":1400,"url":26,"identifiers":1401},"10.1210\u002Fendo-124-5-2172",{"doi":1400},{"id":26,"text":1403,"url":26,"identifiers":1404},"Sabatini M., 1990, Stimulation of tumor necrosis factor release from monocytic cells by the A375 human melanoma via granulocyte‐macrophage colony stimulating factor, Cancer Res, 50, 2673",{},{"id":26,"text":1406,"url":26,"identifiers":1407},"Sabatini M, 1990, Increased production of tumor necrosis factor by normal immune cells in a model of the humoral hypercalcemia of malignancy, Lab Invest, 63, 676",{},{"id":26,"text":1409,"url":26,"identifiers":1410},"Yoneda T., 1989, Hypercalcemia in a human tumor is due to tumor necrosis factor production by host immune cells, J Bone Min Res, 4, 826",{},{"id":1412,"createTime":1413,"updateTime":1413,"relativeEntities":1414,"slug":1415,"properties":1416,"entityType":767,"verifyStatus":25,"verifyTime":1430,"verifyNote":768,"syncStatus":28,"languages":1431,"translateLanguages":26,"viewCount":36,"primaryUrl":1432,"fullTextUrl":26,"authors":1433,"publicationType":836,"publisherRelationship":1522,"citationCount":258,"citationInfo":1554,"publishDate":1556,"publishYear":1557,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":1558,"isForceReanalyzing":932},"25c323d5-6f90-4f2c-9bbc-f7fb78cf12eb","2025-02-06T01:50:46.632+00:00",[],"Povidone-iodine-as-a-periodontal-pocket-disinfectant",{"mag":1417,"keywords":1419,"openalex":1420,"abstract":1422,"title":1424,"pm":1426,"doi":1428},{"VOID":1418},"1992871895",{},{"VOID":1421},"W1992871895",{"EN":1423},"\u003Cjats:p>\u003Cjats:bold>Objectives and background:\u003C\u002Fjats:bold> Povidone‐iodine [polyvinylpyrrolidone‐iodine complex (PVP‐iodine)] might constitute a valuable adjunct to current periodontal therapy because of its broad‐spectrum antimicrobial activity, low potential for developing resistance and adverse reactions, wide availability, ease of use, and low financial cost. This investigation employed a randomized, split‐mouth study design to determine the microbiological and clinical effects of 10% PVP‐iodine subgingival irrigation in periodontitis lesions showing radiographic evidence of subgingival calculus.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Methods:\u003C\u002Fjats:bold> Sixteen adults having at least one periodontal pocket of 6 mm or more in each quadrant of the dentition and harboring one or more periodontopathic bacteria participated in the study. In each subject, a study site in each quadrant was randomly chosen to receive either subgingival irrigation with 10% PVP‐iodine together with scaling and root planing, scaling and root planing alone, subgingival irrigation with 10% PVP‐iodine, or subgingival irrigation with sterile saline. Prior to therapy and at 5 weeks post‐treatment, microbiological culture was carried out without knowledge of the clinical status or the type of treatment rendered. A blinded clinical examiner determined presence of dental plaque, probing pocket depth, and gingival bleeding on probing. Microbiological and clinical data were analyzed using a repeated measures analysis of variance and Kruskal–Wallis rank test with the Tukey and Mann–Whitney \u003Cjats:italic>post hoc\u003C\u002Fjats:italic> tests.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results:\u003C\u002Fjats:bold> At 5 weeks post‐treatment, subgingival irrigation with PVP‐iodine together with scaling and root planing caused a 95% or greater reduction in total pathogen counts in 44% of pockets having ≥ 6 mm depth whereas scaling and root planing alone, povidone‐iodine irrigation alone and water irrigation alone caused 95% reduction of total pathogens only in 6–13% of similar study sites (\u003Cjats:italic>P\u003C\u002Fjats:italic> = 0.02). Reduction in mean pocket depth was 1.8 mm for the PVP‐iodine\u002Fscaling and root planing group, 1.6 mm for the scaling and root planing group, and 0.9 mm for the PVP‐iodine and the saline monotherapy groups, with statistical significance reached for the scaling and root planing group vs. the PVP‐iodine group (\u003Cjats:italic>P\u003C\u002Fjats:italic> = 0.04) and for the scaling and root planing group vs. the saline group (\u003Cjats:italic>P\u003C\u002Fjats:italic> = 0.02). Reduction in visible dental plaque, which ranged from 38% to 62%, showed no significant differences among treatment groups.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusions:\u003C\u002Fjats:bold> The addition of subgingival PVP‐iodine irrigation to conventional mechanical therapy may be a cost‐effective means of reducing total counts of periodontal pathogens and helping control periodontal disease. However, subgingival irrigation with PVP‐iodine without concomitant mechanical debridement might not improve microbiological and clinical variables in comparison with saline irrigation, at least not in sites with radiographic evidence of subgingival calculus.\u003C\u002Fjats:p>",{"EN":1425},"Povidone‐iodine as a periodontal pocket disinfectant",{"VOID":1427},"12753370",{"VOID":1429},"10.1034\u002Fj.1600-0765.2003.02016.x","2025-02-06T01:50:46.631+00:00",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1034\u002Fj.1600-0765.2003.02016.x",[1434,1454,1469,1484,1505],{"id":1435,"sortIndex":115,"researcher":26,"roles":1436,"affiliations":1437,"properties":1449},"ccc2950c-aa47-450b-a42d-309634f822df",[],[1438],{"id":1439,"sortIndex":36,"affiliation":1440,"properties":26},"d6dbf23e-a4e2-49dd-bb2b-4b84e8b5ea50",{"id":1441,"createTime":1442,"updateTime":1443,"relativeEntities":1444,"slug":1445,"properties":1446,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"b1dc0783-f4e9-4fe9-8937-1cebf65f3e4f","2023-12-14T13:47:58.906+00:00","2025-02-06T01:50:46.659+00:00",[],"University-of-Southern-California-School-of-Dentistry-Los-Angeles-CA-USA",{"title":1447},{"VI":1448},"University of Southern California, School of Dentistry, Los Angeles, CA, USA",{"openalex":1450,"title":1452},{"VOID":1451},"A5051069481",{"EN":1453},"Michael G. Jorgensen",{"id":1455,"sortIndex":59,"researcher":26,"roles":1456,"affiliations":1457,"properties":1464},"251be33c-88d8-4623-b30e-d39a0116dd25",[],[1458],{"id":1459,"sortIndex":36,"affiliation":1460,"properties":26},"57ae3838-5b6e-4877-b58b-66da6d0cfc65",{"id":1441,"createTime":1442,"updateTime":1443,"relativeEntities":1461,"slug":1445,"properties":1462,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},[],{"title":1463},{"VI":1448},{"openalex":1465,"title":1467},{"VOID":1466},"A5056042136",{"EN":1468},"Anna Matsuishi 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N, 1990, Diagnostic predictability of scores of plaque, bleeding, suppuration and probing depth for probing attachment loss. 3½ years of observation following initial periodontal therapy, J Clin Periodontol, 17, 108, 10.1111\u002Fj.1600-051X.1990.tb01071.x",{"doi":1568},"10.1111\u002Fj.1600-051X.1990.tb01071.x",{"id":26,"text":1570,"url":26,"identifiers":1571},"10.1902\u002Fjop.1988.59.12.783",{"doi":1570},{"id":26,"text":1573,"url":26,"identifiers":1574},"Tuan MC, 2000, Clinical and microbiological study of periodontal surgery by means of apically positioned flaps with and without osseous recontouring, Int J Periodontics Restorative Dent, 20, 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M, 1993, Bactericidal effects of povidone‐iodine solution to oral pathogenic bacteria in vitro, Postgrad Med J, 69, S10",{},{"id":26,"text":1609,"url":26,"identifiers":1610},"10.1128\u002FAAC.31.12.1989",{"doi":1609},{"id":26,"text":1612,"url":26,"identifiers":1613},"10.1034\u002Fj.1600-0765.2000.035002108.x",{"doi":1612},{"id":26,"text":1615,"url":26,"identifiers":1616},"10.1159\u002F000246027",{"doi":1615},{"id":26,"text":1618,"url":26,"identifiers":1619},"10.1128\u002F9781555817947.ch16",{"doi":1618},{"id":26,"text":1621,"url":26,"identifiers":1622},"10.1159\u002F000057738",{"doi":1621},{"id":26,"text":1624,"url":26,"identifiers":1625},"10.1159\u002F000246024",{"doi":1624},{"id":26,"text":1627,"url":26,"identifiers":1628},"10.1159\u002F000246025",{"doi":1627},{"id":26,"text":1630,"url":26,"identifiers":1631},"10.1159\u002F000246028",{"doi":1630},{"id":26,"text":1633,"url":26,"identifiers":1634},"Juhasz I, 2002, Experiences with the use of povidone‐iodine‐containing local therapeutics in dermatological surgery and in the treatment of burns: testing for allergic sensitization in postsurgery patients, Dermatology, 204, 52, 10.1159\u002F000057726",{"doi":1635},"10.1159\u002F000057726",{"id":26,"text":1637,"url":26,"identifiers":1638},"10.1159\u002F000246038",{"doi":1637},{"id":26,"text":1640,"url":26,"identifiers":1641},"Forabosco A, 1986, A comparative study of a surgical method and scaling and root planing using the Odontoson, J Clin Periodontol, 13, 975",{},{"id":26,"text":1643,"url":26,"identifiers":1644},"10.14219\u002Fjada.archive.1995.0334",{"doi":1643},{"id":26,"text":1646,"url":26,"identifiers":1647},"10.1902\u002Fjop.1999.70.11.1397",{"doi":1646},{"id":26,"text":1649,"url":26,"identifiers":1650},"10.1159\u002F000246022",{"doi":1649},{"id":26,"text":1652,"url":26,"identifiers":1653},"10.1159\u002F000246034",{"doi":1652},{"id":26,"text":1655,"url":26,"identifiers":1656},"Linder N, 1998, Topical iodine‐containing antiseptics and subclinical hypothyroidism in preterm infants, J Pediatr, 133, 309",{},{"id":26,"text":1658,"url":26,"identifiers":1659},"10.14219\u002Fjada.archive.1997.0375",{"doi":1658},{"id":26,"text":1661,"url":26,"identifiers":1662},"Möller ÅJR, 1966, Microbiological examination of root canals and periapical tissues of human teeth, Odontol Tidskr, 74, 1",{},{"id":26,"text":1664,"url":26,"identifiers":1665},"10.1128\u002FJCM.15.4.606-609.1982",{"doi":1664},{"id":26,"text":1667,"url":26,"identifiers":1668},"10.1111\u002Fj.1399-302X.1986.tb00318.x",{"doi":1667},{"id":26,"text":1670,"url":26,"identifiers":1671},"Eccheveria JJ, 1983, Effects of gingival curettage when performed one month after root instrumentation. A biometric evaluation, J Clin Periodontol, 10, 227",{},{"id":26,"text":1673,"url":26,"identifiers":1674},"10.1111\u002Fj.1600-051X.1984.tb00839.x",{"doi":1673},{"id":26,"text":1676,"url":26,"identifiers":1677},"10.1034\u002Fj.1600-051x.2000.027008578.x",{"doi":1676},{"id":26,"text":1679,"url":26,"identifiers":1680},"10.1128\u002FIAI.48.2.534-539.1985",{"doi":1679},{"id":26,"text":1682,"url":26,"identifiers":1683},"American Academy of Periodontology., 1989, Proceedings of the World Workshop in Clinical Periodontics. Section II. Non Surgical Periodontal Treatment., 17",{},{"id":26,"text":1685,"url":26,"identifiers":1686},"10.1902\u002Fjop.2000.71.3.376",{"doi":1685},{"id":26,"text":1688,"url":26,"identifiers":1689},"10.1902\u002Fannals.2001.6.1.138",{"doi":1688},{"id":26,"text":1691,"url":26,"identifiers":1692},"10.1902\u002Fjop.1979.50.10.495",{"doi":1691},{"id":26,"text":1694,"url":26,"identifiers":1695},"10.1111\u002Fj.1600-051X.1984.tb01323.x",{"doi":1694},{"id":26,"text":1697,"url":26,"identifiers":1698},"10.1111\u002Fj.1600-051X.1980.tb01963.x",{"doi":1697},{"id":26,"text":1700,"url":26,"identifiers":1701},"Nakagawa T, 1990, Bactericidal effects on subgingival bacteria of irrigation with a povidone‐iodine solution (Neojodin), Bull Tokyo Dent Coll, 31, 199",{},{"id":26,"text":1703,"url":26,"identifiers":1704},"Okuda K, 1998, The efficacy of antimicrobial mouth rinses in oral health care, Bull Tokyo Dent Coll, 39, 7",{},{"id":26,"text":1706,"url":26,"identifiers":1707},"10.1034\u002Fj.1600-0757.2002.2801123.x",{"doi":1706},{"id":26,"text":1709,"url":26,"identifiers":1710},"10.1902\u002Fjop.1993.64.10.998",{"doi":1709},{"id":26,"text":1712,"url":26,"identifiers":1713},"10.1007\u002Fs007840050050",{"doi":1712},{"id":26,"text":1715,"url":26,"identifiers":1716},"10.1034\u002Fj.1600-0765.2002.00004.x",{"doi":1715},{"id":26,"text":1718,"url":26,"identifiers":1719},"10.1034\u002Fj.1600-0765.2002.01003.x",{"doi":1718},{"id":26,"text":1721,"url":26,"identifiers":1722},"10.1046\u002Fj.1365-2591.2001.00411.x",{"doi":1721},{"id":26,"text":1724,"url":26,"identifiers":1725},"Cigana F, 1991, [A clinical and histological study of the efficacy of betadine on gingival inflammation], J Biol Buccale, 19, 173",{},{"id":26,"text":1727,"url":26,"identifiers":1728},"10.1177\u002F08959374880020023301",{"doi":1727},{"id":26,"text":1730,"url":26,"identifiers":1731},"10.1111\u002Fj.1600-051X.2001.281106.x",{"doi":1730},{"id":1733,"createTime":1734,"updateTime":1734,"relativeEntities":1735,"slug":1736,"properties":1737,"entityType":767,"verifyStatus":25,"verifyTime":1751,"verifyNote":768,"syncStatus":28,"languages":1752,"translateLanguages":26,"viewCount":36,"primaryUrl":1753,"fullTextUrl":26,"authors":1754,"publicationType":836,"publisherRelationship":1832,"citationCount":1865,"citationInfo":1866,"publishDate":1868,"publishYear":1869,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":1870,"isForceReanalyzing":932},"5a01eb86-d43a-47b3-82c6-e5bef3c93b15","2024-09-20T03:05:03.257+00:00",[],"Expression-of-human-%CE%B2-defensin-3-in-gingival-epithelia",{"mag":1738,"keywords":1740,"openalex":1741,"abstract":1743,"title":1745,"pm":1747,"doi":1749},{"VOID":1739},"2025267297",{},{"VOID":1742},"W2025267297",{"EN":1744},"\u003Cjats:p>\u003Cjats:bold>Objective: \u003C\u002Fjats:bold> This study aimed to investigate the expression patterns of the newly discovered human β‐defensin‐3 (hBD‐3) in human gingiva.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Background: \u003C\u002Fjats:bold> Human β‐defensins (hBDs) are a group of small, broad‐spectrum, cationic antimicrobial peptides. Our recent study showed that the expression levels of hBD‐1 and 2 peptides were associated with periodontal conditions.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Methods: \u003C\u002Fjats:bold> A total of 49 gingival biopsies were collected, including 33 samples from 21 patients with chronic periodontitis and 16 samples from 16 periodontally healthy subjects. The expression of hBD‐3 was detected by immunohistochemistry and \u003Cjats:italic>in situ\u003C\u002Fjats:italic> hybridization. Double staining was undertaken to identify hBD‐3 peptide‐positive cells, using CD‐1a and cytokeratin 20 as markers for Langerhans cells and Merkel cells, respectively.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results: \u003C\u002Fjats:bold> hBD‐3 peptide was detected in 88% of the samples, which was confined to the gingival epithelia. In healthy control subjects, hBD‐3 peptide was more frequently detected in the basal layer as compared to the patients (53% vs. 18%, \u003Cjats:italic>p\u003C\u002Fjats:italic> < 0.05). In patients, hBD‐3 expression extended from the basal layer to the spinous layers (82%), in which hBD‐3 was confined to the basal and deep spinous layers in clinically healthy tissues from patients, whereas it extended to the superficial spinous layers in pocket tissues from patients (0% vs. 50%, \u003Cjats:italic>p\u003C\u002Fjats:italic> < 0.05). In both groups, hBD‐3 peptide was expressed not only in gingival keratinocytes, but also in Langerhans cells and Merkel cells. hBD‐3 transcripts were detected in 90% of the samples and they were confined to the basal and\u002For suprabasal layers of gingival epithelia.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusions: \u003C\u002Fjats:bold> This study shows that hBD‐3 is frequently expressed in gingival epithelia. The appropriate expression of hBD‐3 peptide may contribute to the maintenance of periodontal homeostasis, possibly through its antimicrobial effect and promotion of adaptive immune responses.\u003C\u002Fjats:p>",{"EN":1746},"Expression of human β‐defensin‐3 in gingival epithelia",{"VOID":1748},"16302926",{"VOID":1750},"10.1111\u002Fj.1600-0765.2005.00827.x","2024-09-20T03:05:03.256+00:00",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.2005.00827.x",[1755,1776,1793,1815],{"id":1756,"sortIndex":36,"researcher":26,"roles":1757,"affiliations":1758,"properties":1769},"04e4627f-9f9e-45aa-96b8-e75d860a7168",[],[1759],{"id":1760,"sortIndex":36,"affiliation":1761,"properties":26},"2bcf7423-8e36-4cc9-b998-ee3e8908a962",{"id":1762,"createTime":1763,"updateTime":1763,"relativeEntities":1764,"slug":1765,"properties":1766,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"6e2b9531-c944-426c-8e07-c6352aeb6b7f","2024-09-20T03:05:03.283+00:00",[],"Faculty-of-Dentistry-Periodontology-The-University-of-Hong-Kong-Hong-Kong",{"title":1767},{"EN":1768},"Faculty of Dentistry, Periodontology, The University of Hong Kong, Hong Kong",{"openalex":1770,"orcid":1772,"title":1774},{"VOID":1771},"A5014120734",{"VOID":1773},"https:\u002F\u002Forcid.org\u002F0000-0003-2388-0357",{"EN":1775},"Qian Lü",{"id":1777,"sortIndex":59,"researcher":26,"roles":1778,"affiliations":1779,"properties":1786},"7f3e57b8-0b68-4e98-8697-708249403b4e",[],[1780],{"id":1781,"sortIndex":36,"affiliation":1782,"properties":26},"f2ff61e3-184e-473d-8642-4456267067ea",{"id":1762,"createTime":1763,"updateTime":1763,"relativeEntities":1783,"slug":1765,"properties":1784,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},[],{"title":1785},{"EN":1768},{"openalex":1787,"orcid":1789,"title":1791},{"VOID":1788},"A5100669371",{"VOID":1790},"https:\u002F\u002Forcid.org\u002F0000-0003-4305-1337",{"EN":1792},"LJ Jin",{"id":1794,"sortIndex":114,"researcher":26,"roles":1795,"affiliations":1796,"properties":1808},"f389e45f-6b62-4d7a-8100-a746f1d56283",[],[1797],{"id":1798,"sortIndex":36,"affiliation":1799,"properties":26},"f893f45a-5973-4a0c-8390-5d19cba88831",{"id":1800,"createTime":1801,"updateTime":1802,"relativeEntities":1803,"slug":1804,"properties":1805,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"02a34107-d3ce-4ef0-8386-3a8a75862ea0","2024-09-20T03:05:03.317+00:00","2025-06-11T16:02:03.134+00:00",[],"Department-of-Periodontics-University-of-Washington-School-of-Dentistry-Seattle-Washington-USA",{"title":1806},{"EN":1807},"Department of Periodontics, University of Washington School of Dentistry, Seattle, Washington, USA",{"openalex":1809,"orcid":1811,"title":1813},{"VOID":1810},"A5009466623",{"VOID":1812},"https:\u002F\u002Forcid.org\u002F0000-0002-3559-7383",{"EN":1814},"Richard P. 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and Objective: \u003C\u002Fjats:bold> The aim of the study was to evaluate the relationship between cigarette smoking and periodontal damage in terms of the levels of free radicals and antioxidants.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Material and Methods: \u003C\u002Fjats:bold> Thirty‐five healthy subjects in the age group 25–56 yr and with chronic moderate inflammatory periodontal disease (attachment loss of 3–4 mm) were selected. All subjects were matched with respect to the clinical parameters plaque index, gingival index and attachment loss. Of the 35 subjects, 25 were smokers (smoking a minimum of 15 cigarettes\u002Fday) and 10 were nonsmokers. Smokers were subdivided into three subgroups: group I (10 subjects smoking 15–20 cigarettes\u002Fday); group II (10 subjects smoking 21–30 cigarettes\u002Fday) and group III (five subjects smoking > 50 cigarettes\u002Fday). Gingival tissue (obtained during Modified Widman surgery) and blood samples were collected from each of the subjects and analyzed for the following parameters: lipid peroxide, superoxide dismutase, catalase, glutathione and total thiol.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results: \u003C\u002Fjats:bold> The level of lipid peroxide was lowest in nonsmokers (2.242 ± 0.775 in tissue and 1.352 ± 0.414 in blood) and highest in smokers smoking > 50 cigarettes\u002Fday (6.81 ± 1.971 in tissue and 4.96 ± 0.890 in blood), both in tissue and in blood. The increase was statistically significant in all groups, except in tissue of group I smokers. Catalase showed a similar trend, where the levels increased from 0.245 ± 0.043 in controls to 0.610 ± 0.076 in group III smokers for tissue, and from 0.231 ± 0.040 in controls to 0.568 ± 0.104 in group III smokers for blood. The increase was statistically significant for all groups. Total thiol levels were also higher in smokers than in controls (0.222 ± 0.050 in controls vs. 0.480 ± 0.072 in group III smokers in tissue; 0.297 ± 0.078 in controls vs. 0.617 ± 0.042 in group III smokers in blood). Except for group I in both tissue and blood, the increase was statistically significant. The superoxide dismutase (SOD) level was higher in nonsmokers (2.406 ± 0.477 in tissue and 2.611 ± 0.508 in blood) than in group III smokers (1.072 ± 0.367 in tissue and 0.938 ± 0.367 in blood), both in tissue and in blood, but this was significant only in the case of blood and for group III smokers in tissue. The glutathione level in tissue was consistently lower in smokers than in contols, showing a decrease from 121.208 ± 37.367 in controls to 46.426 ± 14.750 in group III smokers, but the decrease was not significant in group I smokers. In the case of blood, the glutathione level dropped from 262.074 ± 68.751 in controls to 154.242 ± 51.721 in group III smokers, but was statistically significant only for group III smokers.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusion: \u003C\u002Fjats:bold> The study results show that smoking increases the level of free radicals in periodontal tissues, which in turn may be responsible for the destruction seen in periodontal diseases.\u003C\u002Fjats:p>",{"EN":1966},"Levels of lipid peroxides and antioxidants in smokers and nonsmokers",{"VOID":1968},"16953817",{"VOID":1970},"10.1111\u002Fj.1600-0765.2006.00889.x","2024-09-22T14:57:33.701+00:00",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.2006.00889.x",[1975,1997,2012,2032,2049],{"id":1976,"sortIndex":115,"researcher":26,"roles":1977,"affiliations":1978,"properties":1990},"c2316894-c973-46bd-958e-043ac1bdeb58",[],[1979],{"id":1980,"sortIndex":36,"affiliation":1981,"properties":26},"cf0d4c30-0247-4e30-9f96-cdd850d0f108",{"id":1982,"createTime":1983,"updateTime":1984,"relativeEntities":1985,"slug":1986,"properties":1987,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"09e4a824-1b8e-4061-8429-6396d1a1ad62","2024-09-22T14:57:33.728+00:00","2025-06-11T22:49:23.936+00:00",[],"Microbiology-King-George-s-Medical-University-Lucknow-Uttar-Pradesh-India",{"title":1988},{"EN":1989},"Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, India",{"openalex":1991,"orcid":1993,"title":1995},{"VOID":1992},"A5020581174",{"VOID":1994},"https:\u002F\u002Forcid.org\u002F0000-0003-4649-2015",{"EN":1996},"Raj Kumar Singh",{"id":1998,"sortIndex":111,"researcher":26,"roles":1999,"affiliations":2000,"properties":2007},"5f48ead3-0544-4458-a286-ee3da7e6e79d",[],[2001],{"id":2002,"sortIndex":36,"affiliation":2003,"properties":26},"779b0de7-be8c-4e01-813e-a1c87ed7c3c2",{"id":1982,"createTime":1983,"updateTime":1984,"relativeEntities":2004,"slug":1986,"properties":2005,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},[],{"title":2006},{"EN":1989},{"openalex":2008,"title":2010},{"VOID":2009},"A5039923050",{"EN":2011},"Vandana Tewari",{"id":2013,"sortIndex":114,"researcher":26,"roles":2014,"affiliations":2015,"properties":2027},"b919ed29-a6d3-46f2-8de5-217baaa069b6",[],[2016],{"id":2017,"sortIndex":36,"affiliation":2018,"properties":26},"9fee3c9b-ebab-4189-9e27-6b3c625eca17",{"id":2019,"createTime":2020,"updateTime":2021,"relativeEntities":2022,"slug":2023,"properties":2024,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"0e18ee7d-6bff-4514-a659-f5181a3ae967","2024-09-22T14:57:33.717+00:00","2024-12-02T15:13:24.209+00:00",[],"Departments-of-Periodontics",{"title":2025},{"EN":2026},"Departments of Periodontics",{"openalex":2028,"title":2030},{"VOID":2029},"A5086015017",{"EN":2031},"Jaya Dixit",{"id":2033,"sortIndex":36,"researcher":26,"roles":2034,"affiliations":2035,"properties":2042},"c05fb9b2-6220-42b4-9390-7c67142e4033",[],[2036],{"id":2037,"sortIndex":36,"affiliation":2038,"properties":26},"f2a697b7-679d-4da9-bb6a-29d49a9d034d",{"id":2019,"createTime":2020,"updateTime":2021,"relativeEntities":2039,"slug":2023,"properties":2040,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},[],{"title":2041},{"EN":2026},{"openalex":2043,"orcid":2045,"title":2047},{"VOID":2044},"A5101815609",{"VOID":2046},"https:\u002F\u002Forcid.org\u002F0000-0002-7683-1105",{"EN":2048},"N. 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HP, 1972, The role of superoxide anion in the auto‐oxidation of epinephrine and a simple assay for superoxide Dismutase, J Biol Chem, 247, 3170, 10.1016\u002FS0021-9258(19)45228-9",{"doi":2146},"10.1016\u002FS0021-9258(19)45228-9",{"id":26,"text":2148,"url":26,"identifiers":2149},"10.1016\u002FS0076-6879(84)05016-3",{"doi":2148},{"id":26,"text":2151,"url":26,"identifiers":2152},"10.1016\u002F0003-9861(59)90090-6",{"doi":2151},{"id":26,"text":2154,"url":26,"identifiers":2155},"Hu M, Methods in Enzymology, 380",{},{"id":26,"text":2157,"url":26,"identifiers":2158},"10.1016\u002FS0021-9258(19)52451-6",{"doi":2157},{"id":26,"text":2160,"url":26,"identifiers":2161},"Hoidal JR, 1981, Altered oxidative metabolic responses in vitro of alveolar macrophages from asymptomatic cigarette smokers, Am Rev Respir Dis, 123, 85",{},{"id":26,"text":2163,"url":26,"identifiers":2164},"10.1042\u002Fbj1390043",{"doi":2163},{"id":26,"text":2166,"url":26,"identifiers":2167},"Halliwell B, 1989, Free Radical in 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and objective\u003C\u002Fjats:title>\u003Cjats:p>A prevailing dental problem in the periodontal patient is root caries. Specifically, periodontal involvement often results in root surfaces becoming exposed and at risk for this condition. Periodontal therapy often leads to increased gingival recession as well, and the associated increased root caries risk may compromise the long‐term success and survival of periodontally treated teeth.This narrative review will address the topic of root caries in the periodontal patient, focusing on unmet research needs.\u003C\u002Fjats:p>\u003C\u002Fjats:sec>\u003Cjats:sec>\u003Cjats:title>Material and Methods\u003C\u002Fjats:title>\u003Cjats:p>The Medline database was searched to identify items dealing with root caries, in terms of clinical features, diagnosis, pathogenic mechanisms and histopathology, as well as epidemiology, focusing then on the relationship between root caries and periodontal disorders.\u003C\u002Fjats:p>\u003C\u002Fjats:sec>\u003Cjats:sec>\u003Cjats:title>Results\u003C\u002Fjats:title>\u003Cjats:p>Although there is extensive literature on root caries, consensus is lacking regarding certain aspects, such as diagnostic criteria, prevalence within populations and indisputable risk factors. Advancing age could be an aggravating factor in susceptibility to root caries for the periodontal patient; however, definitive evidence in this regard is still missing. Similarly, full awareness of the increased risk of root caries in patients with periodontal disease or long‐term periodontal treatment appears to be still lacking.\u003C\u002Fjats:p>\u003C\u002Fjats:sec>\u003Cjats:sec>\u003Cjats:title>Conclusion\u003C\u002Fjats:title>\u003Cjats:p>Research regarding root caries in age‐specific (elderly) periodontal patients is needed. Improved oral hygiene practices, locally applied preventive measures, good dietary habits and regular dental check‐ups are crucial approaches to prevent both periodontal disease progression and root caries. Periodontal patients with root exposure should follow a strict root caries prevention protocol, as an integral component of their periodontal maintenance therapy.\u003C\u002Fjats:p>\u003C\u002Fjats:sec>",{"EN":2191},"Root caries: a periodontal 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Part II. Anatomical considerations, Int J Periodontics Restorative Dent, 6, 64",{},{"id":26,"text":2942,"url":26,"identifiers":2943},"10.1111\u002Fj.1600-051X.1989.tb01035.x",{"doi":2942},{"id":26,"text":2945,"url":26,"identifiers":2946},"Günay H, 2000, Placement of the preparation line and periodontal health – a prospective 2‐year clinical study, Int J Periodontics Restorative Dent, 20, 171",{},{"id":26,"text":2948,"url":26,"identifiers":2949},"10.1034\u002Fj.1600-051X.2003.01277.x",{"doi":2948},{"id":26,"text":2951,"url":26,"identifiers":2952},"10.1111\u002Fj.1600-051X.2008.01358.x",{"doi":2951},{"id":26,"text":2954,"url":26,"identifiers":2955},"10.1111\u002Fj.1600-051X.1998.tb02430.x",{"doi":2954},{"id":26,"text":2957,"url":26,"identifiers":2958},"10.12968\u002Fdenu.2012.39.2.141",{"doi":2957},{"id":26,"text":2960,"url":26,"identifiers":2961},"10.1016\u002F0003-9969(86)90116-0",{"doi":2960},{"id":26,"text":2963,"url":26,"identifiers":2964},"10.1016\u002F0003-9969(77)90158-3",{"doi":2963},{"id":26,"text":2966,"url":26,"identifiers":2967},"10.1007\u002Fs00520-003-0506-0",{"doi":2966},{"id":26,"text":2969,"url":26,"identifiers":2970},"10.1007\u002Fs00066-005-1333-7",{"doi":2969},{"id":26,"text":2972,"url":26,"identifiers":2973},"10.1177\u002F002203459607502S04",{"doi":2972},{"id":26,"text":2975,"url":26,"identifiers":2976},"Okawa Y, 1993, Prevalence of root caries in a Japanese adult population aged 20–59 years, Bull Tokyo Dent Coll, 34, 107",{},{"id":26,"text":2978,"url":26,"identifiers":2979},"10.1016\u002Fj.cden.2004.10.004",{"doi":2978},{"id":26,"text":2981,"url":26,"identifiers":2982},"Gökalp S, 2012, Root caries in 35–44 and 65–74 year‐olds in Turkey, Community Dent Health, 29, 233",{},{"id":26,"text":2984,"url":26,"identifiers":2985},"10.1590\u002FS1678-77572008000100014",{"doi":2984},{"id":26,"text":2987,"url":26,"identifiers":2988},"Matthews DC, 2012, Oral health status of long‐term care residents‐a vulnerable population, J Can Dent Assoc, 78, c3",{},{"id":26,"text":2990,"url":26,"identifiers":2991},"Ferro R, 2008, Oral health problems and needs in nursing home residents in Northern Italy, Community Dent Health, 25, 231",{},{"id":26,"text":2993,"url":26,"identifiers":2994},"10.1111\u002Fj.1447-0594.2011.00764.x",{"doi":2993},{"id":26,"text":2996,"url":26,"identifiers":2997},"Wennström JL, 2000, Treatment of periodontal disease in older adults, Periodontol, 1998, 106",{},{"id":26,"text":2999,"url":26,"identifiers":3000},"10.1902\u002Fjop.1981.52.12.719",{"doi":2999},{"id":26,"text":3002,"url":26,"identifiers":3003},"10.1563\u002F1548-1336-35.2.52",{"doi":3002},{"id":26,"text":3005,"url":26,"identifiers":3006},"Zaremba ML, 2006, Microorganisms in root carious lesions in adults, Adv Med Sci, 51, 237",{},{"id":26,"text":3008,"url":26,"identifiers":3009},"10.1007\u002Fs10096-009-0743-3",{"doi":3008},{"id":26,"text":3011,"url":26,"identifiers":3012},"Ingle NA, 2010, Oral health related quality of life in adult population attending the outpatient department of a hospital in Chennai, India, J Int Oral Health, 2, 45",{},{"id":26,"text":3014,"url":26,"identifiers":3015},"10.14219\u002Fjada.archive.2000.0281",{"doi":3014},{"id":26,"text":3017,"url":26,"identifiers":3018},"10.1080\u002F00016350310002261",{"doi":3017},{"id":26,"text":3020,"url":26,"identifiers":3021},"Anusavice KJ, 2002, Dental caries: risk assessment and treatment solutions for an elderly population, Compend Contin Educ Dent, 23, 12",{},{"id":26,"text":3023,"url":26,"identifiers":3024},"10.1177\u002F154405910408300810",{"doi":3023},{"id":26,"text":3026,"url":26,"identifiers":3027},"10.1111\u002Fj.1741-2358.2008.00263.x",{"doi":3026},{"id":26,"text":3029,"url":26,"identifiers":3030},"10.1111\u002Fj.1741-2358.1995.tb00130.x",{"doi":3029},{"id":26,"text":3032,"url":26,"identifiers":3033},"10.1902\u002Fjop.2007.060456",{"doi":3032},{"id":26,"text":3035,"url":26,"identifiers":3036},"10.1177\u002F0022034512457373",{"doi":3035},{"id":26,"text":3038,"url":26,"identifiers":3039},"10.14219\u002Fjada.archive.1998.0023",{"doi":3038},{"id":26,"text":3041,"url":26,"identifiers":3042},"Renvert S, 2000, Supportive periodontal therapy, Periodontol, 2004, 179",{},{"id":26,"text":3044,"url":26,"identifiers":3045},"Pepelassi E, 2005, Root caries in periodontally treated patients in relation to their compliance with suggested periodontal maintenance intervals, Compend Contin Educ Dent, 26, 835",{},{"id":26,"text":3047,"url":26,"identifiers":3048},"10.1111\u002Fj.1600-0528.1986.tb01491.x",{"doi":3047},{"id":26,"text":3050,"url":26,"identifiers":3051},"10.1111\u002Fj.1741-2358.2002.00080.x",{"doi":3050},{"id":26,"text":3053,"url":26,"identifiers":3054},"10.1111\u002Fj.1600-0528.1991.tb00170.x",{"doi":3053},{"id":3056,"createTime":3057,"updateTime":3057,"relativeEntities":3058,"slug":3059,"properties":3060,"entityType":767,"verifyStatus":25,"verifyTime":3057,"verifyNote":768,"syncStatus":28,"languages":3074,"translateLanguages":26,"viewCount":36,"primaryUrl":3075,"fullTextUrl":26,"authors":3076,"publicationType":836,"publisherRelationship":3130,"citationCount":253,"citationInfo":3162,"publishDate":3164,"publishYear":3165,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":3166,"isForceReanalyzing":932},"25ba3bb7-c4a6-4471-92af-2a6230faad0a","2025-02-06T17:58:22.761+00:00",[],"Inhibition-of-epithelial-cell-matrix-metalloproteinases-by-tetracyclines",{"mag":3061,"keywords":3063,"openalex":3064,"abstract":3066,"title":3068,"pm":3070,"doi":3072},{"VOID":3062},"2117420688",{},{"VOID":3065},"W2117420688",{"EN":3067},"\u003Cjats:p>The effects of tetracyclines on periodontal epithelial cells were investigated by culturing cells from porcine rests of Malassez in the presence of oxytetracycline, doxycycline or one of two analogues of tetracycline bearing no antimicrobial activity. Matrix metalloproteinase activity produced by the epithelial cells was assayed by quantitation of radioactive gelatin degradation and by gelatin enzymography. The results show that all tested tetracyclines exerted a direct dose‐dependent inhibitory effect on epithelial cell gelatinases. Furthermore, epithelial cells cultured with doxycycline, oxytetracycline and de‐dimethylaminot‐etracycline in concentrations ranging from 1 to 50 μg\u002Fml showed a marked reduction in secreted gelatinase activity when grown in alpha minimum essential medium in the absence of fetal calf serum. Viability of cells following this treatment, measured as lactate dehydrogenase activity released to the cell media, was not affected by the presence of any of these drugs at the concentrations used. Scanning electron microscopy revealed striking morphologic changes of the cells following treatment with tetracyclines in the absence of serum which include rounding, decreased intercellular contacts and increased intercellular spaces. No such effects were seen in cells cultured in the presence of serum. These results provide evidence that periodontal epithelial cells produce matrix metalloproteinases whose activities are inhibited by tetracyclines and their non‐antimicrobial analogues at concentrations present in gingival crevicular fluid following tetracycline therapy. When used as adjuncts in periodontal therapy, tetracyclines may therefore inhibit epithelial cell mediated degradation of basement membrane and subepithelial connective tissue.\u003C\u002Fjats:p>",{"EN":3069},"Inhibition of epithelial cell matrix metalloproteinases by tetracyclines",{"VOID":3071},"8410603",{"VOID":3073},"10.1111\u002Fj.1600-0765.1993.tb01082.x",[102],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0765.1993.tb01082.x",[3077,3096,3115],{"id":3078,"sortIndex":36,"researcher":26,"roles":3079,"affiliations":3080,"properties":3091},"3b49af94-6fd1-4289-bd7e-c6e4ff4ba7cb",[],[3081],{"id":3082,"sortIndex":36,"affiliation":3083,"properties":26},"306ed2e3-af51-41a4-b576-636aaae96dc0",{"id":3084,"createTime":3085,"updateTime":3085,"relativeEntities":3086,"slug":3087,"properties":3088,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"35f4d0c3-d3f7-4b7b-b61b-f2fd9860809c","2025-02-06T17:58:22.779+00:00",[],"Departments-of-Oral-Biology-University-of-British-Columbia-Vancouver-Canada",{"title":3089},{"EN":3090},"Departments of Oral Biology, University of British Columbia, Vancouver, Canada",{"openalex":3092,"title":3094},{"VOID":3093},"A5044757941",{"EN":3095},"Leslie Nip",{"id":3097,"sortIndex":114,"researcher":26,"roles":3098,"affiliations":3099,"properties":3110},"43a473bc-09e4-4fd8-a300-618b817ab429",[],[3100],{"id":3101,"sortIndex":36,"affiliation":3102,"properties":26},"e7464146-647d-4a02-89ed-127d73308902",{"id":3103,"createTime":3104,"updateTime":3104,"relativeEntities":3105,"slug":3106,"properties":3107,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},"75b9f282-ee0b-4097-9801-ce482088da8e","2025-02-06T17:58:22.819+00:00",[],"Departments-of-Oral-Biology-and-Pathology-S-U-N-Y-at-Stony-Brook-New-York-U-S-A-",{"title":3108},{"EN":3109},"Departments of Oral Biology and Pathology, S.U.N.Y. at Stony Brook, New York, U.S.A.",{"openalex":3111,"title":3113},{"VOID":3112},"A5111413407",{"EN":3114},"L M Golub",{"id":3116,"sortIndex":115,"researcher":26,"roles":3117,"affiliations":3118,"properties":3125},"29306424-68a8-430e-bb2d-629d8546fc72",[],[3119],{"id":3120,"sortIndex":36,"affiliation":3121,"properties":26},"ed43d402-9533-49c2-8977-19d0e9ce5148",{"id":3084,"createTime":3085,"updateTime":3085,"relativeEntities":3122,"slug":3087,"properties":3123,"entityType":98,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36},[],{"title":3124},{"EN":3090},{"openalex":3126,"title":3128},{"VOID":3127},"A5109278329",{"EN":3129},"V J Uitto",{"url":26,"publisher":3131,"properties":3156},{"id":659,"createTime":660,"updateTime":661,"relativeEntities":3132,"slug":663,"properties":3133,"entityType":24,"verifyStatus":28,"verifyTime":26,"verifyNote":26,"syncStatus":28,"languages":26,"translateLanguages":26,"viewCount":36,"subjectFields":3139,"manageAffiliations":3140,"indexDatabases":3141,"url":743,"thumbnailPath":26,"statistic":26,"gsStatistic":26,"type":26,"analyzePriority":26},[],{"country":3134,"issn":3135,"introduce":3136,"eissn":3137,"title":3138},{"VOID":666},{"VOID":668},{"EN":670},{"VOID":672},{"EN":674},[],[],[3142,3149],{"id":725,"indexDatabase":3143,"url":740,"indexYears":26,"academicFieldIds":3148,"indexDatabaseRanking":26},{"id":727,"createTime":728,"updateTime":729,"relativeEntities":3144,"label":3145,"description":3146,"key":736,"publicationTags":3147,"standard":26},[],{"EN":732,"VI":732},{"VI":734,"EN":735},[738,739],[742],{"id":706,"indexDatabase":3150,"url":719,"indexYears":720,"academicFieldIds":3155,"indexDatabaseRanking":723},{"id":708,"createTime":709,"updateTime":710,"relativeEntities":3151,"label":3152,"description":3153,"key":716,"publicationTags":3154,"standard":26},[],{"EN":713,"VI":713},{"EN":713,"VI":715},[718],[722],{"volume":3157,"pages":3159,"issue":3161},{"VOID":3158},"28",{"VOID":3160},"379-385",{"VOID":1110},{"total":253,"publishYear":26,"statisticByYear":3163},{"2012":59,"2013":114,"2014":59,"2015":59,"2016":115,"2017":115,"2018":59,"2019":114,"2021":59,"2024":114},"1993-09-01",1993,[3167,3170,3173,3176,3179,3181,3184,3187,3190,3193,3196,3199,3202,3205,3208,3211,3215,3219,3222,3225,3228,3231,3234,3237,3240,3243,3246,3249,3252,3255,3258,3261,3264,3268,3271,3274,3277,3280,3284,3287,3291,3295,3298,3301,3304,3307,3310],{"id":26,"text":3168,"url":26,"identifiers":3169},"10.1111\u002Fj.1600-051X.1983.tb02179.x",{"doi":3168},{"id":26,"text":3171,"url":26,"identifiers":3172},"10.1902\u002Fjop.1982.53.9.557",{"doi":3171},{"id":26,"text":3174,"url":26,"identifiers":3175},"Lindhe J, 1982, Host‐parasite interactions in periodontal diseases, 382",{},{"id":26,"text":3177,"url":26,"identifiers":3178},"10.1111\u002Fj.1600-051X.1983.tb01297.x",{"doi":3177},{"id":26,"text":1691,"url":26,"identifiers":3180},{"doi":1691},{"id":26,"text":3182,"url":26,"identifiers":3183},"Fasciano RW, 1981, Periodontal regeneration with long term tetracyclines therapy, Quint Int, 10, 1081",{},{"id":26,"text":3185,"url":26,"identifiers":3186},"10.1902\u002Fjop.1986.57.1.29",{"doi":3185},{"id":26,"text":3188,"url":26,"identifiers":3189},"10.1111\u002Fj.1600-051X.1990.tb01682.x",{"doi":3188},{"id":26,"text":3191,"url":26,"identifiers":3192},"10.1902\u002Fjop.1982.53.10.604",{"doi":3191},{"id":26,"text":3194,"url":26,"identifiers":3195},"10.1902\u002Fjop.1991.62.10.628",{"doi":3194},{"id":26,"text":3197,"url":26,"identifiers":3198},"10.1177\u002F00220345870660080401",{"doi":3197},{"id":26,"text":3200,"url":26,"identifiers":3201},"10.1177\u002F10454411910020030201",{"doi":3200},{"id":26,"text":3203,"url":26,"identifiers":3204},"Lynch WS, 1978, Pyoderma gangrenosum responsive to minocycline hydrochloride, Cutis, 21, 535",{},{"id":26,"text":3206,"url":26,"identifiers":3207},"10.1111\u002F1523-1747.ep12610281",{"doi":3206},{"id":26,"text":3209,"url":26,"identifiers":3210},"10.1016\u002FS0140-6736(89)92555-5",{"doi":3209},{"id":26,"text":3212,"url":26,"identifiers":3213},"Humbert P, 1989, Tetracyclines for dystrophic epidermolysis bullosa, The Lancet, 277, 10.1016\u002FS0140-6736(89)90461-3",{"doi":3214},"10.1016\u002FS0140-6736(89)90461-3",{"id":26,"text":3216,"url":26,"identifiers":3217},"Humbert P, 1991, Use of anticollagenase properties of doxycycline in treatment of alphaantitrypsin deficiency panniculitis, Acta Derm Venerol, 71, 189, 10.2340\u002F0001555571189194",{"doi":3218},"10.2340\u002F0001555571189194",{"id":26,"text":3220,"url":26,"identifiers":3221},"10.3109\u002F00016358709097542",{"doi":3220},{"id":26,"text":3223,"url":26,"identifiers":3224},"10.1016\u002F0006-2952(88)90159-1",{"doi":3223},{"id":26,"text":3226,"url":26,"identifiers":3227},"10.1111\u002Fj.1365-2133.1987.tb04104.x",{"doi":3226},{"id":26,"text":3229,"url":26,"identifiers":3230},"10.1111\u002Fj.1600-0765.1983.tb00388.x",{"doi":3229},{"id":26,"text":3232,"url":26,"identifiers":3233},"10.1111\u002Fj.1600-0765.1984.tb01334.x",{"doi":3232},{"id":26,"text":3235,"url":26,"identifiers":3236},"10.1111\u002Fj.1600-0765.1990.tb00923.x",{"doi":3235},{"id":26,"text":3238,"url":26,"identifiers":3239},"Uitto V‐J, 1992, Development of an in‐vitro model for periodontal epithelium, J Dent Res, 71, 714",{},{"id":26,"text":3241,"url":26,"identifiers":3242},"10.1016\u002F0003-9969(76)90001-7",{"doi":3241},{"id":26,"text":3244,"url":26,"identifiers":3245},"10.1902\u002Fjop.1983.54.12.740",{"doi":3244},{"id":26,"text":3247,"url":26,"identifiers":3248},"10.1016\u002F0003-2697(84)90199-4",{"doi":3247},{"id":26,"text":3250,"url":26,"identifiers":3251},"Bergmeyer HU, 1975, Neue Werte für die molaren Extinktions‐Koeffizienten von NADH und NADPH zum Gebrauch im Routine‐Laboratorium, Z Klin Chem Klin Biochem, 13, 507",{},{"id":26,"text":3253,"url":26,"identifiers":3254},"Katsumoto T, 1984, The effect of tannic acid on the preservation of tissue culture cells for scanning electron microscopy, J Electron Microsc, 30, 177",{},{"id":26,"text":3256,"url":26,"identifiers":3257},"Kinane DF, 1992, Metalloproteinases in the pathogenesis of periodontal diseases, Periodontol Restor Dent, 25",{},{"id":26,"text":3259,"url":26,"identifiers":3260},"10.1016\u002F0003-9969(90)90156-5",{"doi":3259},{"id":26,"text":3262,"url":26,"identifiers":3263},"10.1111\u002Fj.1600-0765.1990.tb00914.x",{"doi":3262},{"id":26,"text":3265,"url":26,"identifiers":3266},"Salo T, 1991, Transforming growth factor‐βl up‐regulates type IV collagenase expression in cultured human keratinocytes, J Biol Chem, 266, 11436, 10.1016\u002FS0021-9258(18)98977-5",{"doi":3267},"10.1016\u002FS0021-9258(18)98977-5",{"id":26,"text":3269,"url":26,"identifiers":3270},"10.1091\u002Fmbc.2.8.613",{"doi":3269},{"id":26,"text":3272,"url":26,"identifiers":3273},"10.1016\u002F0012-1606(67)90029-2",{"doi":3272},{"id":26,"text":3275,"url":26,"identifiers":3276},"10.1016\u002F0005-2744(71)90014-3",{"doi":3275},{"id":26,"text":3278,"url":26,"identifiers":3279},"10.1177\u002F00220345690480050701",{"doi":3278},{"id":26,"text":3281,"url":26,"identifiers":3282},"Lin H‐Y, 1987, Degradation of type 1 collagen by rat mucosal keratinocytes, J Biol Chem, 14, 6823, 10.1016\u002FS0021-9258(18)48319-6",{"doi":3283},"10.1016\u002FS0021-9258(18)48319-6",{"id":26,"text":3285,"url":26,"identifiers":3286},"10.1016\u002FS0934-8832(11)80226-X",{"doi":3285},{"id":26,"text":3288,"url":26,"identifiers":3289},"Seltzer JL, 1989, Cleavage of type VII collagen by interstitial collagenases and type IV collagenases (gelatinases) derived from human skin, J Biol Chem, 264, 3822, 10.1016\u002FS0021-9258(19)84924-4",{"doi":3290},"10.1016\u002FS0021-9258(19)84924-4",{"id":26,"text":3292,"url":26,"identifiers":3293},"Tonetti M, 1990, Zero‐order delivery with periodontal placement of tetracycline‐loaded ethylene vinyl acetate fibers, J Periodont Res, 25, 243, 10.1111\u002Fj.1600-0765.1990.tb00911.x",{"doi":3294},"10.1111\u002Fj.1600-0765.1990.tb00911.x",{"id":26,"text":3296,"url":26,"identifiers":3297},"10.1902\u002Fjop.1992.63.10.849",{"doi":3296},{"id":26,"text":3299,"url":26,"identifiers":3300},"Zucker S, 1989, Gelatin‐degrading type‐IV collagenase isolated from human small cell lung cancer, Invas Metast, 9, 167",{},{"id":26,"text":3302,"url":26,"identifiers":3303},"Zucker S, 1985, Diversity of melanoma plasma membrane proteinases: inhibition of collagenolytic and cytolytic activities by minocycline, J Natl Cancer Inst, 75, 517",{},{"id":26,"text":3305,"url":26,"identifiers":3306},"Yu LP, 1992, Doxycycline inhibits type XI collagenolytic activity of extracts from human osteoarthritic cartilage and of gelatinase, J Rheumatol, 18, 1450",{},{"id":26,"text":3308,"url":26,"identifiers":3309},"Lee HM, 1992, Doxycycline inhibits PMN‐mediated tissue breakdown in culture, J Dent Res, 71, 1118",{},{"id":26,"text":3311,"url":26,"identifiers":3312},"10.1177\u002F10454411910020030301",{"doi":3311},{"id":3314,"createTime":3315,"updateTime":3315,"relativeEntities":3316,"slug":3317,"properties":3318,"entityType":767,"verifyStatus":25,"verifyTime":3332,"verifyNote":768,"syncStatus":28,"languages":3333,"translateLanguages":26,"viewCount":36,"primaryUrl":3334,"fullTextUrl":26,"authors":3335,"publicationType":836,"publisherRelationship":3388,"citationCount":249,"citationInfo":3419,"publishDate":3421,"publishYear":1115,"citationAnalyzeStatus":28,"lastCitationAnalyze":26,"indexDatabases":26,"openAccess":26,"references":3422,"isForceReanalyzing":932},"d0b41e42-60bd-467a-b10e-a86f03fce0ba","2024-09-11T20:50:59.039+00:00",[],"Identification-of-genes-related-to-mechanical-stress-in-human-periodontal-ligament-cells-using-microarray-analysis",{"mag":3319,"keywords":3321,"openalex":3322,"abstract":3324,"title":3326,"pm":3328,"doi":3330},{"VOID":3320},"2053207460",{},{"VOID":3323},"W2053207460",{"EN":3325},"\u003Cjats:p>\u003Cjats:bold>Background and Objective: \u003C\u002Fjats:bold> Differential expression of genes in human periodontal ligament (PDL) under mechanical stress, such as orthodontic force, is thought to be involved in the remodeling of PDL cells and periodontal tissues. However, little is known about the genes expressed in PDL cells under mechanical stress.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Material and Methods: \u003C\u002Fjats:bold> We employed microarray analysis to assess, in a comprehensive manner, the gene expression profiles in PDL cells compressed by a static force using an \u003Cjats:italic>in vitro\u003C\u002Fjats:italic> three‐dimensional culture system. Six genes were selected and validated by quantitative real‐time polymerase chain reaction analysis, consistent with the microarray data.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results: \u003C\u002Fjats:bold> The microarray data revealed that 108 of 30,000 genes tested were differentially expressed by mechanical force loading. Among them, 85 genes were up‐regulated by mechanical stress, while 23 genes were down‐regulated, judging by the thresholds of a two‐fold increase\u002Fdecrease compared with the controls. Thirty‐two of the up‐regulated and eight of the down‐regulated genes, well‐characterized in protein function, were involved in numerous biological processes including cell communication, cell signaling, cell cycle, stress response, and calcium release. However, several genes differentially expressed in our microarray data have not been well defined as stress‐response molecules.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusion: \u003C\u002Fjats:bold> Our microarray is the first to show the gene profile in PDL cells caused by mechanical stress; however, further studies to clarify the physiological function of these molecules in PDL cells are required.\u003C\u002Fjats:p>",{"EN":3327},"Identification of genes related to mechanical stress in human periodontal ligament cells using microarray 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To establish a method for quantification of \u003Cjats:italic>Actinobacillus actinomycetemcomitans\u003C\u002Fjats:italic> and \u003Cjats:italic>Porphyromonas gingivalis\u003C\u002Fjats:italic> from subgingival plaque by real‐time polymerase chain reaction (PCR) technique.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Material and methods: \u003C\u002Fjats:bold> Bacterial cells from both species were obtained from type culture and counted microscopically. Cellular suspension in sterile destilled water was used for DNA extraction by boiling for 20 min, with a mineral oil cover. Primers for PCR were selected from sequences of LktC gene \u003Cjats:italic>(A. actinomycetemcomitans)\u003C\u002Fjats:italic> and Arg‐gingipain \u003Cjats:italic>(P. gingivalis)\u003C\u002Fjats:italic> to yield amplicons below 100 bp. SYBR Green I based real‐time PCR was adjusted to quantify separately both species.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Results: \u003C\u002Fjats:bold> A good sensitivity and specificity were obtained for both species, although the yield was better for \u003Cjats:italic>A. actinomycetemcomitans\u003C\u002Fjats:italic>. A good repeatibility of cycle threshold (\u003Cjats:italic>C\u003C\u002Fjats:italic>\u003Cjats:sub>T\u003C\u002Fjats:sub>) was encountered, so coefficient of variation was below 6% at every initial copy number.\u003C\u002Fjats:p>\u003Cjats:p>\u003Cjats:bold>Conclusion: \u003C\u002Fjats:bold> A new method of quantification of \u003Cjats:italic>A. actinomycetemcomitans\u003C\u002Fjats:italic> and \u003Cjats:italic>P. gingivalis\u003C\u002Fjats:italic> based on SYBR Green real‐time PCR is presented. Its good sensibility and repeatibility will allow its application to analysis of subgingival plaque samples.\u003C\u002Fjats:p>",{"EN":3604},"Quantitative real‐time PCR based on single copy gene sequence for detection of \u003Ci>Actinobacillus actinomycetemcomitans\u003C\u002Fi> and \u003Ci>Porphyromonas 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