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Epithelial‐specific knockout of the\u003C\u002Fjats:italic> Rac1 \u003Cjats:italic>gene leads to enamel defects. \u2028Eur J Oral Sci 2011; 119 (Suppl. 1): 168–176. © 2011 Eur J Oral Sci\u003C\u002Fjats:italic>\u003C\u002Fjats:p>\u003Cjats:p>The Ras‐related C3 botulinum toxin substrate 1 (\u003Cjats:italic>Rac1\u003C\u002Fjats:italic>) gene encodes a 21‐kDa GTP‐binding protein belonging to the RAS superfamily. RAS members play important roles in controlling focal adhesion complex formation and cytoskeleton contraction, activities with consequences for cell growth, adhesion, migration, and differentiation. To examine the role(s) played by RAC1 protein in cell–matrix interactions and enamel matrix biomineralization, we used the \u003Cjats:italic>Cre\u003C\u002Fjats:italic>\u002F\u003Cjats:italic>loxP\u003C\u002Fjats:italic> binary recombination system to characterize the expression of enamel matrix proteins and enamel formation in \u003Cjats:italic>Rac1\u003C\u002Fjats:italic> knockout mice (\u003Cjats:italic>Rac1\u003C\u002Fjats:italic>\u003Cjats:sup>\u003Cjats:italic>−\u002F−\u003C\u002Fjats:italic>\u003C\u002Fjats:sup>). Mating between mice bearing the floxed \u003Cjats:italic>Rac1\u003C\u002Fjats:italic> allele and mice bearing a cytokeratin 14‐\u003Cjats:italic>Cre\u003C\u002Fjats:italic> transgene generated mice in which \u003Cjats:italic>Rac1\u003C\u002Fjats:italic> was absent from epithelial organs. Enamel of the \u003Cjats:italic>Rac1\u003C\u002Fjats:italic> conditional knockout mouse was characterized by light microscopy, backscattered electron imaging in the scanning electron microscope, microcomputed tomography, and histochemistry. Enamel matrix protein expression was analyzed by western blotting. Major findings showed that the Tomes’ processes of \u003Cjats:italic>Rac1\u003C\u002Fjats:italic>\u003Cjats:sup>\u003Cjats:italic>−\u002F−\u003C\u002Fjats:italic>\u003C\u002Fjats:sup> ameloblasts lose contact with the forming enamel matrix in unerupted teeth, the amounts of amelogenin and ameloblastin are reduced in \u003Cjats:italic>Rac1\u003C\u002Fjats:italic>\u003Cjats:sup>\u003Cjats:italic>−\u002F−\u003C\u002Fjats:italic>\u003C\u002Fjats:sup> ameloblasts, and after eruption, the enamel from \u003Cjats:italic>Rac1\u003C\u002Fjats:italic>\u003Cjats:sup>\u003Cjats:italic>−\u002F−\u003C\u002Fjats:italic>\u003C\u002Fjats:sup> mice displays severe structural defects with a complete loss of enamel. 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measures are often preferred to examine outcomes of oral disorders. However, generic measures can add additional information, including health utility. The aim was to assess the Oral Health Impact Profile (\u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content>), EuroQol (\u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D), and Assessment of Quality of Life (\u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL) instruments in relation to oral health in terms of their discriminative and convergent validities. Data were collected from adults, 30–61 yr of age, in Australia by mailed survey during 2009 and 2010, including the \u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content>‐14, the \u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D, and the \u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL, a range of self‐reported oral health variables, and by self‐rated oral and general health. Responses were collected from 1093 subjects (a response rate of 39.1%). The \u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content>, the \u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D, and the \u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL were associated with oral health variables, with effect sizes ranging from 0.6 to 1.1 for the \u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content>, from 0.3 to 0.5 for the \u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D, and from 0.4 to 0.6 for the \u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL. The \u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content> tended to be more strongly correlated with self‐rated oral health (rho = −0.5) than with general health (rho = −0.3), whilst the \u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D and the \u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL were less strongly correlated with oral health (rho = −0.3 and −0.3, respectively) than with general health (rho = −0.4 and −0.5, respectively). Whilst the \u003Cjats:styled-content style=\"fixed-case\">OHIP\u003C\u002Fjats:styled-content> was more sensitive to differences in oral health, the generic measures of \u003Cjats:styled-content style=\"fixed-case\">EQ\u003C\u002Fjats:styled-content>‐5D and \u003Cjats:styled-content style=\"fixed-case\">AQ\u003C\u002Fjats:styled-content>oL both exhibited discriminative validity and convergent validity in relation to oral health variables, supporting their use in oral health studies.\u003C\u002Fjats:p>",{"EN":1005},"Oral Health Impact Profile, \u003Cscp>E\u003C\u002Fscp>uro\u003Cscp>Q\u003C\u002Fscp>ol, and Assessment of Quality of Life instruments as quality of life and health‐utility measures of oral health",{"VOID":1007},"23659242",{"VOID":1009},"10.1111\u002Feos.12035",[127],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Feos.12035",[1013],{"id":1014,"sortIndex":25,"researcher":24,"roles":1015,"affiliations":1016,"properties":1033,"displayName":1037,"givenName":24,"familyName":24},"914c5d77-e87f-4377-beca-fa8c782f3dce",[],[1017,1025],{"id":1018,"sortIndex":25,"affiliation":1019,"properties":24},"22b667b6-60c8-42a7-8ff2-419e771d8dda",{"id":1018,"createTime":24,"updateTime":24,"relativeEntities":1020,"slug":24,"properties":1021,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":1024,"statistic":24},[],{"title":1022},{"EN":1023},"Australian Research Centre for Population Oral Health, School of Dentistry, Adelaide, Australia",[],{"id":1026,"sortIndex":89,"affiliation":1027,"properties":24},"7a9e89e5-3516-47bb-8710-a92ff1efe8ec",{"id":1026,"createTime":24,"updateTime":24,"relativeEntities":1028,"slug":24,"properties":1029,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":1032,"statistic":24},[],{"title":1030},{"EN":1031},"David S. 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With regard to oral implantology, patient situations of an edentulous maxilla and mandible as well as a single tooth gap in regions 16 and 46 were simulated. Radiation doses were measured between 0.2 and 22.5 mGy to organs and tissues in the head and neck region when the complete maxilla or mandible was examined. When examining a single tooth gap, only 8% to 40% of that radiation dose was generally observed. Based on these results, the mortality risk was estimated according to a calculation model recommended by the \u003Cjats:italic>Committee on the Biological Effects of Ionizing Radiations.\u003C\u002Fjats:italic> The mortality risk ranged from 31.4 × 10\u003Cjats:sup>−6\u003C\u002Fjats:sup> for 20‐year‐old men to 4.8 × 10\u003Cjats:sup>−6\u003C\u002Fjats:sup> for 65‐year‐old women when cross‐sectional imaging of the complete maxilla was performed. The values decreased by 70% when a single tooth gap in the molar region of the maxilla was radiographed. The figures for the mortality risk for examinations of the complete mandible were similar to those for the complete maxilla, but the mortality risk decreased by 80% if only a single tooth gap in the molar region of the mandible was examined. Calculations according to the \u003Cjats:italic>International Commission on Radiological Protection\u003C\u002Fjats:italic> carried out for comparison did not reveal the decrease of the mortality risk with age and resulted in a higher risk value in comparison to the group of 35‐year old individuals in calculations according to the \u003Cjats:italic>Committee on the Biological Effects of Ionizing Radiations.\u003C\u002Fjats:italic>\u003C\u002Fjats:p>",{"EN":1217},"Hypothetical mortality risk associated with spiral tomography of the maxilla and mandible prior to endosseous implant treatment",{"VOID":1219},"9151064",{"VOID":1221},"10.1111\u002Fj.1600-0722.1997.tb00190.x",[127],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0722.1997.tb00190.x",[1225,1250,1267,1284,1301,1316,1331,1346],{"id":1226,"sortIndex":25,"researcher":24,"roles":1227,"affiliations":1228,"properties":1245,"displayName":1247,"givenName":24,"familyName":24},"4d9f507c-f44e-44cb-887e-331bbed18c0c",[],[1229,1237],{"id":1230,"sortIndex":25,"affiliation":1231,"properties":24},"fd542c11-5f73-4e38-acf4-2638ff683d16",{"id":1230,"createTime":24,"updateTime":24,"relativeEntities":1232,"slug":24,"properties":1233,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":1236,"statistic":24},[],{"title":1234},{"EN":1235},"Department of Oral Radiology, School of Dentistry, Academic Center for Dentistry Amsterdam, Amsterdam, The Netherlands",[],{"id":1238,"sortIndex":89,"affiliation":1239,"properties":24},"15eace1b-2e72-4b1e-a9e9-47e04b197947",{"id":1238,"createTime":24,"updateTime":24,"relativeEntities":1240,"slug":24,"properties":1241,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":1244,"statistic":24},[],{"title":1242},{"EN":1243},"Department of Oral Surgery, Section of Dental Radiology and Diagnostic Stomatology, School of Dental Medicine, University of Berne, Berne, Switzerland",[],{"title":1246,"openalex":1248},{"EN":1247},"Karl Dula",{"VOID":1249},"A5061442956",{"id":1251,"sortIndex":89,"researcher":24,"roles":1252,"affiliations":1253,"properties":1262,"displayName":1264,"givenName":24,"familyName":24},"9949efc3-fed0-4b48-9daf-4a494ee51515",[],[1254],{"id":1255,"sortIndex":25,"affiliation":1256,"properties":24},"e8b46861-68fc-4daa-998b-c4530c07edf8",{"id":1255,"createTime":24,"updateTime":24,"relativeEntities":1257,"slug":24,"properties":1258,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":1261,"statistic":24},[],{"title":1259},{"EN":1260},"Department of Radio-oncology, Division of Medical Radiation Physics, School of Medicine, University of Berne, Berne, Switzerland",[],{"title":1263,"openalex":1265},{"EN":1264},"R. 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et dispositifs de radiographie sur plaque en mouvement. 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The measurements were carried out with 2 phantoms, a head and neck phantom and a full body phantom. The analysis of applied thermoluminescent dosimeters yielded radiation doses for organs and tissues in the head and neck region between 0.6 and 16.7 mGy when 40 axial slices and 120 kV\u002F165 mAs were used as exposure parameters. The effective dose was calculated as 0.58 and 0.48 mSv in the maxilla and mandible, respectively. Tested methods for dose reduction showed a significant decrease of radiation dose from 40 to 65%. Based on these results, the mortality risk was estimated according to calculation models recommended by the \u003Cjats:italic>Committee on the Biological Effects of Ionizing Radiations and by the International Commission on Radiological Protection.\u003C\u002Fjats:italic> Both models resulted in similar values. The mortality risk ranges from 46.2×10‐6 for 20‐year‐old men to 11.2×10\u003Cjats:sup>−6\u003C\u002Fjats:sup> for 65‐year‐old women. Using 2 methods of dose reduction, the mortality risk decreased by ∼ 50 to 60% to 19.1×10\u003Cjats:sup>−6\u003C\u002Fjats:sup> for 20‐year‐old men and 5.5×10\u003Cjats:sup>−6\u003C\u002Fjats:sup> for 65‐year‐old women. It can be concluded that a CT scan of the maxillofacial complex causes a considerable radiation dose when compared with conventional radiographic examinations. 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purpose of this study was to investigate the condensation pressure during amalgam placement in patients. Forty‐four practitioners were asked to fill two class 2 cavities in the lower jaw on their patients in their own practice with the aid of a force‐measuring plugger. This device, with semiconductor strain gauges, allowed the dentist to have an identical tactile feedback as with any other plugger. Three different amalgams, with different shapes of alloy particles, were tested. The results showed a maximum condensation pressure of 8.9±2.4 MPa and 5.5±1.8 MPa with a small and a large amalgam plugger, respectively. Average condensation pressures were 3.7±1.3 MPa for the small and 2.2±0.9 MPa for the large instrument. The total working time required to fill a cavity was on average 131 s; the amalgam was effectively condensed for 44 s. No significant differences between amalgams with different shapes of alloy particles, no influence of time of day, and no difference between female and male dentists were found. This study showed that the condensation pressure is lower than often recommended, and that it is not statistically different from the values obtained in a previous laboratory study (1).\u003C\u002Fjats:p>",{"EN":2019},"Condensation pressure during amalgam placement in patients",{"VOID":2021},"8747675",{"VOID":2023},"10.1111\u002Fj.1600-0722.1995.tb01862.x",[127],"https:\u002F\u002Fonlinelibrary.wiley.com\u002Fdoi\u002F10.1111\u002Fj.1600-0722.1995.tb01862.x",[2027,2044,2061,2076],{"id":2028,"sortIndex":25,"researcher":24,"roles":2029,"affiliations":2030,"properties":2039,"displayName":2041,"givenName":24,"familyName":24},"c24fc41b-2561-4d59-ac68-3f45b38bd2b8",[],[2031],{"id":2032,"sortIndex":25,"affiliation":2033,"properties":24},"d48be194-5354-4c52-a710-a5476e10c130",{"id":2032,"createTime":24,"updateTime":24,"relativeEntities":2034,"slug":24,"properties":2035,"entityType":24,"verifyStatus":24,"verifyTime":24,"verifyNote":24,"languages":24,"translateLanguages":24,"viewCount":24,"url":24,"parentIds":2038,"statistic":24},[],{"title":2036},{"EN":2037},"Department of Operative, Preventive and Pediatric Dentistry, University of Bern, School of Dental Medicine, Freiburgstrasse 7, CH – 3010 Bern, 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AE, 1981, The influence of condensing pressure on the strength of three dental amalgams, Operative Dent, 6, 6",{},{"id":24,"text":2159,"url":24,"identifiers":2160},"10.3109\u002F00016358309162343",{"doi":2159},{"id":24,"text":2162,"url":24,"identifiers":2163},"10.1016\u002F0022-3913(84)90094-5",{"doi":2162},{"id":24,"text":2165,"url":24,"identifiers":2166},"10.1111\u002Fj.1365-2842.1987.tb00702.x",{"doi":2165},{"id":24,"text":2168,"url":24,"identifiers":2169},"Rebel H., 1950, Konservierende Zahnheilkunde, 139",{},{"id":24,"text":2171,"url":24,"identifiers":2172},"Jørgensen KD., 1977, Amalgame in der Zahnheilkunde",{},{"id":24,"text":2174,"url":24,"identifiers":2175},"Mayer R., 1984, Amalgam‐Kondensation – maschinell oder manuell, Dtsch Zahnärztl Z, 39, 736",{},{"id":24,"text":2177,"url":24,"identifiers":2178},"10.14219\u002Fjada.archive.1965.0099",{"doi":2177},{"id":24,"text":2180,"url":24,"identifiers":2181},"Basker RM, 1968, Condensation of amalgam. The clinical measurement of forces and rates of packing, Br Dent J, 124, 451",{},{"id":24,"text":2183,"url":24,"identifiers":2184},"Brown IH, 1993, Variation in condensation pressure during clinical packing of amalgam restorations, Am J Dent, 6, 255",{},{"id":24,"text":2186,"url":24,"identifiers":2187},"10.1038\u002Fsj.bdj.4802666",{"doi":2186},{"id":24,"text":2189,"url":24,"identifiers":2190},"Hegdahl T, 1970, Porosity of spherical amalgam, Scand J Dent Res, 78, 62",{},{"id":24,"text":2192,"url":24,"identifiers":2193},"10.1016\u002F0022-3913(85)90548-7",{"doi":2192},{"id":24,"text":2195,"url":24,"identifiers":2196},"10.14219\u002Fjada.archive.1967.0160",{"doi":2195},{"id":24,"text":2198,"url":24,"identifiers":2199},"Brown IH, 1993, Alloy particle shape and sensitivity of high‐copper amalgams to manipulative variables, Am J Dent, 6, 248",{},{"id":24,"text":2201,"url":24,"identifiers":2202},"10.3109\u002F00016358009003482",{"doi":2201},{"id":24,"text":2204,"url":24,"identifiers":2205},"Osborne WJ., 1990, Three year clinical performance of eight amalgam alloys, Am J Dent, 3, 157",{},{"id":24,"text":2207,"url":24,"identifiers":2208},"Lehmann G., 1965, Arbeitsrnedizin I, 349",{},{"id":2210,"createTime":2211,"updateTime":2212,"relativeEntities":2213,"slug":2214,"properties":2215,"entityType":122,"verifyStatus":123,"verifyTime":2211,"verifyNote":125,"languages":2232,"translateLanguages":2233,"viewCount":25,"primaryUrl":2235,"fullTextUrl":24,"authors":2236,"publicationType":199,"publisherRelationship":2330,"citationCount":2380,"citationInfo":2381,"publishDate":2384,"publishYear":2382,"citationAnalyzeStatus":23,"lastCitationAnalyze":24,"indexDatabases":2385,"openAccess":24,"references":2386,"isForceReanalyzing":261},"f7c6bc9e-7470-43f8-b511-d460d8d53ed4","2024-12-17T19:28:28.859+00:00","2024-12-25T23:54:33.926+00:00",[],"Toothache-prevalence-and-associated-factors-a-life-course-study-from-birth-to-age-12-yr",{"mag":2216,"keywords":2218,"openalex":2220,"abstract":2222,"title":2225,"pm":2228,"doi":2230},{"VOID":2217},"2164670259",{"VI":2219},"đau răng, tần suất, yếu tố liên quan, nghiên cứu cuộc sống, chỉ số dmf-t, chỉ số DMF-T, kinh tế-xã hội, bối cảnh gia đình, Brazil, trẻ em",{"VOID":2221},"W2164670259",{"EN":2223,"VI":2224},"\u003Cjats:p>This study estimated the lifetime prevalence of toothache at ages 6 and 12 yr, the prevalence of toothache during the last month, and their association with social, behavioural and clinical exposures in the course of life of 339 12‐yr‐old children from a birth cohort in Pelotas, Brazil. Exploratory variables were collected in the perinatal study and during several follow‐up studies. Prevalence ratios were calculated using Poisson regression, following a hierarchical conceptual model. The lifetime prevalence of toothache at ages 6 and 12 yr were 39% [95% confidence interval (CI) = (34;45)] and 63% [95% CI = (58;69)], respectively. Toothache during the last month was reported by 11% [95% CI = (8;15)]. Children who did not live with their biological father at birth, and children with higher dmf‐t counts, reported a higher lifetime prevalence of toothache at age 6 yr. Children experiencing poverty between ages 0 and 4 yr, with higher dmf‐t and DMF‐T indexes presented a greater lifetime prevalence of toothache at 12 yr. Toothache within the last month was more likely to be reported by girls and by children who did not live with their biological father at birth. Preventive strategies should be implemented in early stages of the life cycle, taking into account the socio‐economic and family context in which pain mostly occurs.\u003C\u002Fjats:p>","\u003Cjats:p>Nghiên cứu này ước tính tỷ lệ đau răng trọn đời ở độ tuổi 6 và 12 tuổi, tỷ lệ đau răng trong tháng vừa qua và sự liên quan của chúng với các yếu tố xã hội, hành vi và lâm sàng trong suốt cuộc đời của 339 trẻ 12 tuổi từ một đoàn hệ sinh ở Pelotas, Brazil. Các biến số khám phá được thu thập từ nghiên cứu chu sinh và trong nhiều nghiên cứu theo dõi. Các tỷ lệ thịnh hành được tính toán bằng hồi quy Poisson, theo một mô hình khái niệm phân cấp. Tỷ lệ đau răng trọn đời ở độ tuổi 6 và 12 tuổi lần lượt là 39% [khoảng tin cậy 95% (CI) = (34;45)] và 63% [95% CI = (58;69)]. Đau răng trong tháng qua được báo cáo bởi 11% [95% CI = (8;15)]. Trẻ không sống cùng cha ruột khi sinh và trẻ có chỉ số dmf-t cao hơn báo cáo tỷ lệ đau răng trọn đời cao hơn ở độ tuổi 6 tuổi. Trẻ trải qua nghèo đói từ 0 đến 4 năm tuổi, có chỉ số dmf-t và DMF-T cao hơn có tỷ lệ đau răng trọn đời lớn hơn ở độ tuổi 12 tuổi. Đau răng trong tháng qua có khả năng được báo cáo nhiều hơn bởi các bé gái và trẻ không sống với cha ruột khi sinh. 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