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Its bioconversion to 1,3-propanediol (1,3-PDO) is an environmentally friendly method. Continuous fermentation has many striking merits over fed-batch and batch fermentation, such as high product concentration with easy feeding operation, long-term high productivity without frequent seed culture, and energy-intensive sterilization. However, it is usually difficult to harvest high product concentrations.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Results\u003C\u002Fjats:title>\n                \u003Cjats:p>In this study, a three-stage continuous fermentation was firstly designed to produce 1,3-PDO from crude glycerol by \u003Cjats:italic>Clostridium butyricum\u003C\u002Fjats:italic>, in which the first stage fermentation was responsible for providing the excellent cells in a robust growth state, the second stage focused on promoting 1,3-PDO production, and the third stage aimed to further boost the 1,3-PDO concentration and reduce the residual glycerol concentration as much as possible. Through the three-stage continuous fermentation, 80.05 g\u002FL 1,3-PDO as the maximum concentration was produced while maintaining residual glycerol of 5.87 g\u002FL, achieving a yield of 0.48 g\u002Fg and a productivity of 3.67 g\u002F(L·h). Based on the 14 sets of experimental data from the first stage, a kinetic model was developed to describe the intricate relationships among the concentrations of 1,3-PDO, substrate, biomass, and butyrate. Subsequently, this kinetic model was used to optimize and predict the highest 1,3-PDO productivity of 11.26 g\u002F(L·h) in the first stage fermentation, while the glycerol feeding concentration and dilution rate were determined to be 92 g\u002FL and 0.341 h\u003Cjats:sup>−1\u003C\u002Fjats:sup>, separately. Additionally, to achieve a target 1,3-PDO production of 80 g\u002FL without the third stage fermentation, the predicted minimum volume ratio of the second fermenter to the first one was 11.9. The kinetics-based two-stage continuous fermentation was experimentally verified well with the predicted results.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Conclusion\u003C\u002Fjats:title>\n                \u003Cjats:p>A novel three-stage continuous fermentation and a kinetic model were reported. Then a simpler two-stage continuous fermentation was developed based on the optimization of the kinetic model. This kinetics-based development of two-stage continuous fermentation could achieve high-level production of 1,3-PDO. Meanwhile, it provides a reference for other bio-chemicals production by applying kinetics to optimize multi-stage continuous fermentation.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Graphical Abstract\u003C\u002Fjats:title>\n                \n              \u003C\u002Fjats:sec>",{"EN":132,"VI":133},"Kinetics-based development of two-stage continuous fermentation of 1,3-propanediol from crude glycerol by Clostridium butyricum","Phát triển dựa trên động học quá trình lên men liên tục hai giai đoạn sản xuất 1,3-propanediol từ glycerol thô bằng Clostridium butyricum",{"VOID":135},"38454489",{"VOID":137},"10.1186\u002Fs13068-024-02486-5","PUBLICATION","VERIFIED","2025-01-21T00:21:42.337+00:00","Auto 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M, Kamarudin SK, Kofli NT. The potential of glycerol as a value-added commodity. Chem Eng J. 2016;295:119–30. https:\u002F\u002Fdoi.org\u002F10.1016\u002Fj.cej.2016.03.012.",{"doi":288},"10.1016\u002Fj.cej.2016.03.012",{"id":18,"text":290,"url":18,"identifiers":291},"Barbirato F, Himmi EH, Conte T, Bories A. 1,3-Propanediol production by fermentation: an interesting way to valorize glycerin from the ester and ethanol industries. Ind Crop Prod. 1998;7:281–9. https:\u002F\u002Fdoi.org\u002F10.1016\u002FS0926-6690(97)00059-9.",{"doi":292},"10.1016\u002FS0926-6690(97)00059-9",{"id":18,"text":294,"url":18,"identifiers":295},"Chilakamarry CR, Sakinah AMM, Zularisam AW, Pandey A. Glycerol waste to value added products and its potential applications. Syst Microbiol Biomanuf. 2021;1:378–96. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs43393-021-00036-w.",{"doi":296},"10.1007\u002Fs43393-021-00036-w",{"id":18,"text":298,"url":18,"identifiers":299},"Fokum E, Zabed HM, Yun J, Zhang G, Qi X. Recent technological and strategical developments in the biomanufacturing of 1,3-propanediol from glycerol. Int J Environ Sci Technol. 2021;18:2467–90. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs13762-020-03036-w.",{"doi":300},"10.1007\u002Fs13762-020-03036-w",{"id":18,"text":302,"url":18,"identifiers":303},"Ruan M, Luan H, Wan G, Shen M. Bio-polyols synthesized from bio-based 1,3-propanediol and applications on polyurethane reactive hot melt adhesives. Ind Crop Prod. 2019;128:436–44. https:\u002F\u002Fdoi.org\u002F10.1016\u002Fj.indcrop.2018.11.045.",{"doi":304},"10.1016\u002Fj.indcrop.2018.11.045",{"id":18,"text":306,"url":18,"identifiers":307},"Martins FF, Liberato VDSS, Ribeiro CMS, Coelho MAZ, Ferreira TF. Low-cost medium for 1,3-propanediol production from crude glycerol by Clostridium butyricum. Biofuels Bioprod Biorefin. 2020;14:1125–34. https:\u002F\u002Fdoi.org\u002F10.1002\u002Fbbb.2133.",{"doi":308},"10.1002\u002Fbbb.2133",{"id":18,"text":310,"url":18,"identifiers":311},"Kluge M, Pérocheau Arnaud S, Robert T. 1,3-Propanediol and its application in bio-based polyesters for resin applications. Chem Africa. 2019;2:215–21. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs42250-018-0026-4.",{"doi":312},"10.1007\u002Fs42250-018-0026-4",{"id":18,"text":314,"url":18,"identifiers":315},"Lan Y, Feng J, Guo X, Fu H, Wang J. Isolation and characterization of a newly identified Clostridium butyricum strain SCUT343-4 for 1,3-propanediol production. Bioprocess Biosyst Eng. 2021;44:2375–85. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs00449-021-02610-x.",{"doi":316},"10.1007\u002Fs00449-021-02610-x",{"id":18,"text":318,"url":18,"identifiers":319},"Sun Y, Shen J, Yan L, Zhou J, Jiang L, Chen Y, Yuan J, Feng E, Xiu Z. Advances in bioconversion of glycerol to 1,3-propanediol: prospects and challenges. Process Biochem. 2018;71:134–46. https:\u002F\u002Fdoi.org\u002F10.1016\u002Fj.procbio.2018.05.009.",{"doi":320},"10.1016\u002Fj.procbio.2018.05.009",{"id":18,"text":322,"url":18,"identifiers":323},"Zhu Y, Wang Y, Gao H, Wang H, Wan Z, Jiang Y, Xin F, Zhang W, Jiang M. Current advances in microbial production of 1,3-propanediol. Biofuel Bioprod Biorefin. 2021;15:1566–83. https:\u002F\u002Fdoi.org\u002F10.1002\u002Fbbb.2254.",{"doi":324},"10.1002\u002Fbbb.2254",{"id":18,"text":326,"url":18,"identifiers":327},"Maina S, Kachrimanidou V, Ladakis D, Papanikolaou S, Castro AM, Koutinas A. Evaluation of 1,3-propanediol production by two Citrobacter freundii strains using crude glycerol and soybean cake hydrolysate. Environ Sci Pollut Res. 2019;26:35523–32. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs11356-019-05485-4.",{"doi":328},"10.1007\u002Fs11356-019-05485-4",{"id":18,"text":330,"url":18,"identifiers":331},"Ju J, Wang D, Heo S, Kim M, Seo J, Kim Y, Kim D, Kang S, Kim C, Oh B. Enhancement of 1,3-propanediol production from industrial by-product by Lactobacillus reuteri CH53. Microb Cell Fact. 2020;19:6. https:\u002F\u002Fdoi.org\u002F10.1186\u002Fs12934-019-1275-x.",{"doi":332},"10.1186\u002Fs12934-019-1275-x",{"id":18,"text":334,"url":18,"identifiers":335},"Shen J, Zhou J, Fu H, Mu Y, Sun Y, Xu Y, Xiu Z. A Klebsiella pneumoniae bacteriophage and its effect on 1,3-propanediol fermentation. Process Biochem. 2016;51:1323–30. https:\u002F\u002Fdoi.org\u002F10.1016\u002Fj.procbio.2016.07.026.",{"doi":336},"10.1016\u002Fj.procbio.2016.07.026",{"id":18,"text":338,"url":18,"identifiers":339},"Zhou J, Shen J, Wang X, Sun Y, Xiu Z. 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Biochem Eng J. 2012;68:34–41. https:\u002F\u002Fdoi.org\u002F10.1016\u002Fj.bej.2012.07.004.",{"doi":494},"10.1016\u002Fj.bej.2012.07.004",false,{"id":497,"createTime":498,"updateTime":499,"relativeEntities":500,"slug":501,"properties":502,"entityType":138,"verifyStatus":139,"verifyTime":513,"verifyNote":141,"languages":18,"translateLanguages":18,"viewCount":19,"primaryUrl":514,"fullTextUrl":18,"authors":515,"publicationType":222,"publisherRelationship":545,"citationCount":608,"citationInfo":609,"publishDate":614,"publishYear":610,"citationAnalyzeStatus":615,"lastCitationAnalyze":616,"indexDatabases":617,"openAccess":18,"references":18,"isForceReanalyzing":495},"08dfddb9-515d-48ba-813a-65c80a4bc3f1","2024-02-13T01:29:59.834+00:00","2026-07-29T00:15:17.195+00:00",[],"Detoxification-of-a-pyrolytic-aqueous-condensate-from-wheat-straw-for-utilization-as-substrate-in-Aspergillus-oryzae-DSM-1863-cultivations",{"abstract":503,"title":505,"gsPaper":507,"references":509,"doi":511},{"EN":504},"The pyrolytic aqueous condensate (PAC) formed during the fast pyrolysis of wheat straw contains a variety of organic carbons and might therefore potentially serve as an inexpensive substrate for microbial growth. One of its main components is acetic acid, which was recently shown to be a suitable carbon source for the filamentous fungus Aspergillus oryzae. However, the condensate also contains numerous toxic compounds that inhibit fungal growth and result in a tolerance of only about 1%. Therefore, to enable the use of the PAC as sole substrate for A. oryzae cultivations, a pretreatment seems to be necessary. Various conditions for treatments with activated carbon, overliming, rotary evaporation and laccase were evaluated regarding fungal growth and the content of inhibitory model substances. Whereas the first three methods considerably increased the fungal tolerance to up to 1.625%, 12.5% and 30%, respectively, the enzymatic treatment did not result in any improvement. The optimum carbon load for the treatment with activated carbon was identified to be 10% (w\u002Fv) and overliming should ideally be performed at 100 °C and an initial pH of 12. The best detoxification results were achieved with rotary evaporation at 200 mbar as a complete removal of guaiacol and a strong reduction in the concentration of acetol, furfural, 2-cyclopenten-1-one and phenol by 84.9%, 95.4%, 97.7% and 86.2%, respectively, were observed. Subsequently, all possible combinations of the effective single methods were performed and rotary evaporation followed by overliming and activated carbon treatment proved to be most efficient as it enabled growth in 100% PAC shake-flask cultures and resulted in a maximum cell dry weight of 5.21 ± 0.46 g\u002FL. This study provides a comprehensive insight into the detoxification efficiency of a variety of treatment methods at multiple conditions. It was revealed that with a suitable combination of these methods, PAC toxicity can be reduced to such an extent that growth on pure condensate is possible. 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Curr Protoc Food Anal Chem. 2002;6(1):I1. 1.1-8.",{"VOID":512},"10.1186\u002Fs13068-022-02115-z","2024-08-31T01:27:00.740+00:00","https:\u002F\u002Fbiotechnologyforbiofuels.biomedcentral.com\u002Farticles\u002F10.1186\u002Fs13068-022-02115-z",[516,532],{"id":517,"sortIndex":19,"researcher":18,"roles":518,"affiliations":520,"properties":529,"displayName":531,"givenName":18,"familyName":18},"fa0a4fa4-6a13-49aa-a5ca-ba409ffda8a3",[519],"AUTHOR",[521],{"id":522,"sortIndex":19,"affiliation":523,"properties":18},"b5db10d6-0099-4ccf-970f-43e718456f80",{"id":522,"createTime":18,"updateTime":18,"relativeEntities":524,"slug":18,"properties":525,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":528,"statistic":18},[],{"title":526},{"VI":527},"Institute of Process Engineering in Life Sciences 2-Technical Biology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany",[],{"title":530},{"VI":531},"Christin Kubisch",{"id":533,"sortIndex":112,"researcher":18,"roles":534,"affiliations":535,"properties":542,"displayName":544,"givenName":18,"familyName":18},"cb559fd4-db8b-4dca-a872-2b65fc428e11",[519],[536],{"id":522,"sortIndex":19,"affiliation":537,"properties":18},{"id":522,"createTime":18,"updateTime":18,"relativeEntities":538,"slug":18,"properties":539,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":541,"statistic":18},[],{"title":540},{"VI":527},[],{"title":543},{"VI":544},"Katrin 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lignocellulose-to-biofuel biorefinery process that enables multiple product streams is recognized as a promising strategy to improve the economics of this biorefinery and to accelerate technology commercialization. We recently identified an innovative pretreatment technology that enables of the production of sugars at high yields while simultaneously generating a high-quality lignin stream that has been demonstrated as both a promising renewable polyol replacement for polyurethane applications and is highly susceptible to depolymerization into monomers. This technology comprises a two-stage pretreatment approach that includes an alkaline pre-extraction followed by a metal-catalyzed alkaline-oxidative pretreatment. Our recent work demonstrated that H2O2 and O2 act synergistically as co-oxidants during the alkaline-oxidative pretreatment and could significantly reduce the pretreatment chemical input while maintaining high sugar yields (~ 95% glucose and ~ 100% xylose of initial sugar composition), high lignin yields (~ 75% of initial lignin), and improvements in lignin usage. This study considers the economic impact of these advances and provides strategies that could lead to additional economic improvements for future commercialization. The results of the technoeconomic analysis (TEA) demonstrated that adding O2 as a co-oxidant at 50 psig for the alkaline-oxidative pretreatment and reducing the raw material input reduced the minimum fuel selling price from $1.08\u002FL to $0.85\u002FL, assuming recoverable lignin is used as a polyol replacement. If additional lignin can be recovered and sold as more valuable monomers, the minimum fuel selling price (MFSP) can be further reduced to $0.73\u002FL. The present work demonstrated that high sugar and lignin yields combined with low raw material inputs and increasing the value of lignin could greatly increase the economic viability of a poplar-based biorefinery. Continued research on integrating sugar production with lignin valorization is thus warranted to confirm this economic potential as the technology matures.",{"EN":628},"Technoeconomic evaluation of recent process improvements in production of sugar and high-value lignin co-products via two-stage Cu-catalyzed alkaline-oxidative pretreatment",{"VOID":630},"[]",{"VOID":632},"Pinales-Márquez CD, Rodríguez-Jasso RM, Araújo RG, Loredo-Treviño A, Nabarlatz D, Gullón B, Ruiz HA. Circular bioeconomy and integrated biorefinery in the production of xylooligosaccharides from lignocellulosic biomass: a review. Ind Crops Prod. 2021;162: 113274.\nSoltanian S, Aghbashlo M, Almasi F, Hosseinzadeh-Bandbafha H, Nizami AS, Ok YS, Lam SS, Tabatabaei M. A critical review of the effects of pretreatment methods on the exergetic aspects of lignocellulosic biofuels. Energy Conver Manag. 2020;212: 112792.\nYoo CG, Meng X, Pu Y, Ragauskas AJ. 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Ind Eng Chem Res. 2019;58:16000–8.",{"VOID":634},"10.1186\u002Fs13068-022-02139-5","2024-05-17T13:46:24.609+00:00","https:\u002F\u002Fbiotechnologyforbiofuels.biomedcentral.com\u002Farticles\u002F10.1186\u002Fs13068-022-02139-5",[638,653,668,681],{"id":639,"sortIndex":19,"researcher":18,"roles":640,"affiliations":641,"properties":650,"displayName":652,"givenName":18,"familyName":18},"0465fc83-f222-4804-bff9-981379e6ba20",[519],[642],{"id":643,"sortIndex":19,"affiliation":644,"properties":18},"a38aab3d-d87a-434c-9cbf-8a7c689b83ac",{"id":643,"createTime":18,"updateTime":18,"relativeEntities":645,"slug":18,"properties":646,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":649,"statistic":18},[],{"title":647},{"VI":648},"Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, USA",[],{"title":651},{"VI":652},"Zhaoyang Yuan",{"id":654,"sortIndex":112,"researcher":18,"roles":655,"affiliations":656,"properties":665,"displayName":667,"givenName":18,"familyName":18},"1b2446be-fbb4-4a2a-93b8-76a1759c2749",[519],[657],{"id":658,"sortIndex":19,"affiliation":659,"properties":18},"4b4d7935-8c4a-464c-9382-628fd31b2033",{"id":658,"createTime":18,"updateTime":18,"relativeEntities":660,"slug":18,"properties":661,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":664,"statistic":18},[],{"title":662},{"VI":663},"Michigan Biotechnology Institute, Lansing, USA",[],{"title":666},{"VI":667},"Bryan D. Bals",{"id":669,"sortIndex":186,"researcher":18,"roles":670,"affiliations":671,"properties":678,"displayName":680,"givenName":18,"familyName":18},"6b7a0b32-d25b-4eb9-becf-b83bcb36deb6",[519],[672],{"id":643,"sortIndex":19,"affiliation":673,"properties":18},{"id":643,"createTime":18,"updateTime":18,"relativeEntities":674,"slug":18,"properties":675,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":677,"statistic":18},[],{"title":676},{"VI":648},[],{"title":679},{"VI":680},"Eric L. Hegg",{"id":682,"sortIndex":206,"researcher":18,"roles":683,"affiliations":684,"properties":702,"displayName":704,"givenName":18,"familyName":18},"c75f48be-fcdf-47b8-8ccf-853b53892c34",[519],[685,693],{"id":686,"sortIndex":19,"affiliation":687,"properties":18},"6751eaaf-f0e5-4ebf-a227-6bb33ae1d171",{"id":686,"createTime":18,"updateTime":18,"relativeEntities":688,"slug":18,"properties":689,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":692,"statistic":18},[],{"title":690},{"VI":691},"Department of Chemical & Biological Engineering, Montana State University, Bozeman, USA",[],{"id":694,"sortIndex":112,"affiliation":695,"properties":701},"87bddbb2-72aa-49f4-a265-4ac16e55e348",{"id":694,"createTime":18,"updateTime":18,"relativeEntities":696,"slug":18,"properties":697,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":700,"statistic":18},[],{"title":698},{"VI":699},"Division of Sustainable Process Engineering, Luleå University of Technology, Luleå, Sweden",[],{},{"title":703},{"VI":704},"David B. Hodge",{"url":636,"publisher":706,"properties":763},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":707,"slug":10,"properties":708,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":711,"manageAffiliations":732,"indexDatabases":743,"url":106,"thumbnailPath":18,"statistic":758,"gsStatistic":18,"type":115,"analyzePriority":18},[],{"issn":709,"title":710},{"VOID":13},{"EN":15},[712,716,720,724,728],{"id":22,"createTime":18,"updateTime":18,"relativeEntities":713,"label":714,"description":715,"parentId":18,"standard":18,"scholarHubFieldId":18},[],{"EN":25},{},{"id":28,"createTime":18,"updateTime":18,"relativeEntities":717,"label":718,"description":719,"parentId":18,"standard":18,"scholarHubFieldId":18},[],{"EN":31},{},{"id":34,"createTime":18,"updateTime":18,"relativeEntities":721,"label":722,"description":723,"parentId":18,"standard":18,"scholarHubFieldId":18},[],{"EN":37},{},{"id":40,"createTime":18,"updateTime":18,"relativeEntities":725,"label":726,"description":727,"parentId":18,"standard":18,"scholarHubFieldId":18},[],{"EN":43},{},{"id":46,"createTime":18,"updateTime":18,"relativeEntities":729,"label":730,"description":731,"parentId":18,"standard":18,"scholarHubFieldId":18},[],{"EN":49},{},[733,738],{"id":53,"createTime":18,"updateTime":18,"relativeEntities":734,"slug":18,"properties":735,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":737,"statistic":18},[],{"title":736},{"EN":57},[],{"id":60,"createTime":18,"updateTime":18,"relativeEntities":739,"slug":18,"properties":740,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":742,"statistic":18},[],{"title":741},{"EN":64},[],[744,751],{"id":68,"indexDatabase":745,"url":79,"indexYears":80,"academicFieldIds":750,"indexDatabaseRanking":87},{"id":70,"createTime":18,"updateTime":18,"relativeEntities":746,"label":747,"description":748,"key":76,"publicationTags":749,"standard":18},[],{"EN":73,"VI":73},{"EN":73,"VI":75},[78],[82,83,84,85,86],{"id":89,"indexDatabase":752,"url":102,"indexYears":18,"academicFieldIds":757,"indexDatabaseRanking":18},{"id":91,"createTime":18,"updateTime":18,"relativeEntities":753,"label":754,"description":755,"key":98,"publicationTags":756,"standard":18},[],{"EN":94,"VI":94},{"EN":96,"VI":97},[100,101],[104,105],{"impactFactor":19,"impactFactorByYear":759,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":109,"totalPublicationByYear":760,"totalCitation":19,"totalCitationByYear":761,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":762,"hindexLast5Year":19,"hindex":19},{},{"2023":111,"2024":112},{},{},{"pages":764,"volume":766},{"VOID":765},"1-12",{"VOID":607},"2022-05-04","ERROR_IN_GET_PLATFORM_ID","2026-06-17T22:56:34.458+00:00",[78,100],{"id":772,"createTime":773,"updateTime":774,"relativeEntities":775,"slug":776,"properties":777,"entityType":138,"verifyStatus":139,"verifyTime":788,"verifyNote":141,"languages":18,"translateLanguages":18,"viewCount":19,"primaryUrl":789,"fullTextUrl":18,"authors":790,"publicationType":222,"publisherRelationship":978,"citationCount":19,"citationInfo":1041,"publishDate":1044,"publishYear":1042,"citationAnalyzeStatus":17,"lastCitationAnalyze":1045,"indexDatabases":1046,"openAccess":18,"references":18,"isForceReanalyzing":495},"c414b465-1d23-4220-809c-712af44cfbe1","2024-02-18T23:25:39.170+00:00","2026-04-21T07:02:59.864+00:00",[],"Marine-cyanobacterial-biomass-is-an-efficient-feedstock-for-fungal-bioprocesses",{"abstract":778,"title":780,"gsPaper":782,"references":784,"doi":786},{"EN":779},"Marine cyanobacteria offer many sustainability advantages, such as the ability to fix atmospheric CO2, very fast growth and no dependence on freshwater for culture. Cyanobacterial biomass is a rich source of sugars and proteins, two essential nutrients for culturing any heterotroph. However, no previous study has evaluated their application as a feedstock for fungal bioprocesses. In this work, we cultured the marine cyanobacterium Synechococcus sp. PCC 7002 in a 3-L externally illuminated bioreactor with working volume of 2 L with a biomass productivity of ~ 0.8 g L−1 day−1. Hydrolysis of the biomass with acids released proteins and hydrolyzed glycogen while hydrolysis of the biomass with base released only proteins but did not hydrolyze glycogen. Among the different acids tested, treatment with HNO3 led to the highest release of proteins and glucose. Cyanobacterial biomass hydrolysate (CBH) prepared in HNO3 was used as a medium to produce cellulase enzyme by the Penicillium funiculosum OAO3 strain while CBH prepared in HCl and treated with charcoal was used as a medium for citric acid by Aspergillus tubingensis. Approximately 50% higher titers of both products were obtained compared to traditional media. These results show that the hydrolysate of marine cyanobacteria is an effective source of nutrients\u002Fproteins for fungal bioprocesses.",{"EN":781},"Marine cyanobacterial biomass is an efficient feedstock for fungal bioprocesses",{"VOID":783},"[\"9785755992941544417\"]",{"VOID":785},"CO2 emissions—Global Energy Review 2021—Analysis—IEA. https:\u002F\u002Fwww.iea.org\u002Freports\u002Fglobal-energy-review-2021\u002Fco2-emissions.\nSVS: Global Carbon Dioxide 2020–2021. https:\u002F\u002Fsvs.gsfc.nasa.gov\u002Fcgi-bin\u002Fdetails.cgi?aid=4949&button=recent. Accessed 19 Jan 2024.\nOcean acidification | National Oceanic and Atmospheric Administration. https:\u002F\u002Fwww.noaa.gov\u002Feducation\u002Fresource-collections\u002Focean-coasts\u002Focean-acidification. 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Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungus Penicillium funiculosum NCIM1228. Biotechnol Biofuels. 2018;11:1–22. https:\u002F\u002Fdoi.org\u002F10.1186\u002Fs13068-018-1011-5.\nUpton DJ, McQueen-Mason SJ, Wood AJ. An accurate description of Aspergillus niger organic acid batch fermentation through dynamic metabolic modelling. Biotechnol Biofuels. 2017;10:1–14. https:\u002F\u002Fdoi.org\u002F10.1186\u002Fs13068-017-0950-6.\nGhose TK. Measurement of cellulase activities. Pure Appl Chem. 1987;59:257–68. https:\u002F\u002Fdoi.org\u002F10.1351\u002Fpac198759020257\u002Fhtml.\nKamennaya NA, Ahn SE, Park H, Bartal R, Sasaki KA, Holman HY, et al. Installing extra bicarbonate transporters in the cyanobacterium Synechocystis sp. PCC6803 enhances biomass production. Metab Eng. 2015;29:76–85.\nWłodarczyk A, Selão TT, Norling B, Nixon PJ. Newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain for high biomass production. Commun Biol. 2020;3:1–14.\nPathania R, Srivastava S. Synechococcus elongatus BDU 130192, an attractive cyanobacterium for feedstock applications: response to culture conditions. Bioenergy Res. 2021;14:954–63. https:\u002F\u002Fdoi.org\u002F10.1007\u002Fs12155-020-10207-7.",{"VOID":787},"10.1186\u002Fs13068-024-02469-6","2024-05-08T10:47:18.869+00:00","https:\u002F\u002Fbiotechnologyforbiofuels.biomedcentral.com\u002Farticles\u002F10.1186\u002Fs13068-024-02469-6",[791,816,840,863,878,900,913,935,957],{"id":792,"sortIndex":19,"researcher":18,"roles":793,"affiliations":794,"properties":811,"displayName":813,"givenName":18,"familyName":18},"e47cf7fc-b069-4ed1-84d9-3965faa8dcc8",[519],[795,803],{"id":796,"sortIndex":19,"affiliation":797,"properties":18},"9c434fd7-3458-418d-81e8-7f83dc3282a6",{"id":796,"createTime":18,"updateTime":18,"relativeEntities":798,"slug":18,"properties":799,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":802,"statistic":18},[],{"title":800},{"VI":801},"Systems Biology for Biofuel Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India",[],{"id":804,"sortIndex":112,"affiliation":805,"properties":18},"526f855b-b108-4b30-ae81-66802532a546",{"id":804,"createTime":18,"updateTime":18,"relativeEntities":806,"slug":18,"properties":807,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":810,"statistic":18},[],{"title":808},{"VI":809},"Zero Cow Factory, Surat, India",[],{"title":812,"gsAuthor":814},{"VI":813},"Jai Kumar Gupta",{"VOID":815},"[\"v-1_QFoAAAAJ\"]",{"id":817,"sortIndex":112,"researcher":18,"roles":818,"affiliations":819,"properties":837,"displayName":839,"givenName":18,"familyName":18},"8f68a235-bd32-4496-9b58-6427a7930d89",[519],[820,828],{"id":821,"sortIndex":19,"affiliation":822,"properties":18},"a6e6d82a-9ddb-4b87-a215-a23a867bcf1d",{"id":821,"createTime":18,"updateTime":18,"relativeEntities":823,"slug":18,"properties":824,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":827,"statistic":18},[],{"title":825},{"VI":826},"DBT-ICGEB Centre for Advanced Bioenergy Research, New Delhi, India",[],{"id":829,"sortIndex":112,"affiliation":830,"properties":836},"f89d69fb-adcd-427c-a600-c2c900452e91",{"id":829,"createTime":18,"updateTime":18,"relativeEntities":831,"slug":18,"properties":832,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":835,"statistic":18},[],{"title":833},{"VI":834},"The Live Green Co., Bangalore, India",[],{},{"title":838},{"VI":839},"Kavish K. 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are emerging hosts for the sustainable production of lutein, a high-value carotenoid; however, to be commercially competitive with existing systems, their capacity for lutein sequestration must be augmented. Previous attempts to boost microalgal lutein production have focussed on upregulating carotenoid biosynthetic enzymes, in part due to a lack of metabolic engineering targets for expanding lutein storage. Here, we isolated a lutein hyper-producing mutant of the model green microalga Chlamydomonas reinhardtii and characterized the metabolic mechanisms driving its enhanced lutein accumulation using label-free quantitative proteomics. Norflurazon- and high light-resistant C. reinhardtii mutants were screened to yield four mutant lines that produced significantly more lutein per cell compared to the CC-125 parental strain. Mutant 5 (Mut-5) exhibited a 5.4-fold increase in lutein content per cell, which to our knowledge is the highest fold increase of lutein in C. reinhardtii resulting from mutagenesis or metabolic engineering so far. Comparative proteomics of Mut-5 against its parental strain CC-125 revealed an increased abundance of light-harvesting complex-like proteins involved in photoprotection, among differences in pigment biosynthesis, central carbon metabolism, and translation. Further characterization of Mut-5 under varying light conditions revealed constitutive overexpression of the photoprotective proteins light-harvesting complex stress-related 1 (LHCSR1) and LHCSR3 and PSII subunit S regardless of light intensity, and increased accrual of total chlorophyll and carotenoids as light intensity increased. Although the photosynthetic efficiency of Mut-5 was comparatively lower than CC-125, the amplitude of non-photochemical quenching responses of Mut-5 was 4.5-fold higher than in CC-125 at low irradiance. We used C. reinhardtii as a model green alga and identified light-harvesting complex-like proteins (among others) as potential metabolic engineering targets to enhance lutein accumulation in microalgae. These have the added value of imparting resistance to high light, although partially compromising photosynthetic efficiency. Further genetic characterization and engineering of Mut-5 could lead to the discovery of unknown players in photoprotective mechanisms and the development of a potent microalgal lutein production system.","Vi tảo đang nổi lên như là các đối tượng tiềm năng cho sản xuất bền vững lutein, một loại carotenoid giá trị cao; tuy nhiên, để cạnh tranh thương mại với các hệ thống hiện có, khả năng giữ lutein của chúng cần phải được tăng cường. Những nỗ lực trước đó nhằm nâng cao sản xuất lutein ở vi tảo tập trung vào việc tăng cường các enzyme sinh tổng hợp carotenoid, một phần do thiếu các mục tiêu kỹ thuật di truyền để mở rộng kho dự trữ lutein. Trong nghiên cứu này, chúng tôi đã phân lập một đột biến sản xuất lutein cao của vi tảo xanh mô hình Chlamydomonas reinhardtii và đặc trưng hóa các cơ chế chuyển hóa thúc đẩy sự tích lũy lutein tăng cường của nó thông qua proteomics định lượng không nhãn. Các đột biến C. reinhardtii chống lại norflurazon và ánh sáng mạnh đã được sàng lọc để cho ra bốn dòng đột biến sản xuất lutein nhiều hơn đáng kể so với chủng cha CC-125. Đột biến 5 (Mut-5) thể hiện sự tăng 5.4 lần về hàm lượng lutein trên mỗi tế bào, đây là mức tăng cao nhất của lutein trong C. reinhardtii do đột biến hoặc kỹ thuật di truyền cho đến nay. Proteomics so sánh của Mut-5 với chủng cha CC-125 cho thấy sự gia tăng số lượng protein giống như phức hợp thu nhận ánh sáng tham gia vào bảo vệ ánh sáng, bên cạnh những khác biệt trong sinh tổng hợp sắc tố, chuyển hóa carbon trung tâm và dịch mã. Việc đặc trưng hóa thêm Mut-5 dưới các điều kiện ánh sáng khác nhau cho thấy sự biểu hiện quá mức các protein bảo vệ ánh sáng như phức hợp thu nhận ánh sáng liên quan đến stress 1 (LHCSR1) và LHCSR3 và tiểu đơn vị PSII S không phụ thuộc vào cường độ ánh sáng, cũng như sự gia tăng tích lũy chlorophyll tổng cộng và carotenoid khi cường độ ánh sáng tăng lên. Mặc dù hiệu suất quang hợp của Mut-5 tương đối thấp hơn so với CC-125, độ cao của phản ứng giảm ánh sáng không quang hóa của Mut-5 cao hơn 4.5 lần so với CC-125 ở cường độ ánh sáng thấp. Chúng tôi đã sử dụng C. reinhardtii như một vi tảo xanh mô hình và xác định các protein giống như phức hợp thu nhận ánh sáng (trong số những cái khác) như là các mục tiêu kỹ thuật di truyền tiềm năng để tăng cường sự tích lũy lutein trong vi tảo. Những protein này cũng có giá trị bổ sung trong việc làm tăng tính kháng với ánh sáng mạnh, mặc dù có thể làm giảm hiệu suất quang hợp. Việc đặc trưng hóa và kỹ thuật di truyền thêm cho Mut-5 có thể dẫn đến việc phát hiện ra các yếu tố chưa biết trong các cơ chế bảo vệ ánh sáng và phát triển một hệ thống sản xuất lutein vi tảo hiệu quả.",{"EN":1058,"VI":1059},"Proteomic characterization of a lutein-hyperaccumulating Chlamydomonas reinhardtii mutant reveals photoprotection-related factors as targets for increasing cellular carotenoid content","Đặc điểm proteomic của đột biến Chlamydomonas reinhardtii tích lũy lutein cao cho thấy các yếu tố liên quan đến bảo vệ ánh sáng là mục tiêu để tăng cường hàm lượng carotenoid trong tế bào",{"VI":1061},"vitảo, lutein, carotenoid, Chlamydomonas reinhardtii, bảo vệ ánh sáng",{"VOID":1063},"Wijffels RH, Kruse O, Hellingwerf KJ. Potential of industrial biotechnology with cyanobacteria and eukaryotic microalgae. Curr Opin Biotechnol. 2013;24:405–13.\nGangl D, Zedler JAZ, Rajakumar PD, Martinez EMR, Riseley A, Włodarczyk A, et al. Biotechnological exploitation of microalgae. 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Production of phytoene by herbicide-treated microalgae Dunaliella bardawil in two-phase systems. Biotechnol Bioeng. 2005;92(6):695–701.\nBarera S, Dall’Osto L, Bassi R. Effect of lhcsr gene dosage on oxidative stress and light use efficiency by Chlamydomonas reinhardtii cultures. J Biotechnol. 2021;328:12–22.\nCroce R, Canino G, Ros F, Bassi R. Chromophore organization in the higher-plant photosystem II antenna protein CP26. Biochemistry. 2002;41(23):7334–43.\nHitchcock A, Jackson PJ, Chidgey JW, Dickman MJ, Hunter CN, Canniffe DP. Biosynthesis of chlorophyll a in a purple bacterial phototroph and assembly into a plant chlorophyll-protein complex. ACS Synth Biol. 2016;5(9):948–54.\nCox J, Hein MY, Luber CA, Paron I, Nagaraj N, Mann M. Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ. Mol Cell Proteomics. 2014;13(9):2513–26.\nSchwacke R, Ponce-Soto GY, Krause K, Bolger AM, Arsova B, Hallab A, et al. MapMan4: a refined protein classification and annotation framework applicable to multi-omics data analysis. Mol Plant. 2019;12(6):879–92.\nGoodstein DM, Shu S, Howson R, Neupane R, Hayes RD, Fazo J, et al. Phytozome: a comparative platform for green plant genomics. Nucleic Acids Res. 2012;40(D1):D1178–86.\nThe UniProt Consortium. UniProt: the universal protein knowledgebase in 2023. Nucleic Acids Res. 2023;51(D1):D523–31.\nCutolo E, Tosoni M, Barera S, Herrera-Estrella L, Dall’Osto L, Bassi R. A chimeric hydrolase-PTXD transgene enables chloroplast-based heterologous protein expression and non-sterile cultivation of Chlamydomonas reinhardtii. Algal Res. 2021;1(59):102429.\nVan Kooten O, Snel JFH. The use of chlorophyll fluorescence nomenclature in plant stress physiology. 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Hanover: Dartmouth College; 1995.",{"doi":1732},"10.1349\u002Fddlp.2757",{"id":18,"text":1734,"url":18,"identifiers":1735},"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215:403–10.",{"doi":1736},"10.1016\u002FS0022-2836(05)80360-2",{"id":18,"text":1738,"url":18,"identifiers":1739},"Scopes RK. An iron-activated alcohol dehydrogenase. FEBS Lett. 1983;156:303–6.",{"doi":1740},"10.1016\u002F0014-5793(83)80517-1",{"id":18,"text":1742,"url":18,"identifiers":1743},"Lamed R, Zeikus JG. Ethanol production by thermophilic bacteria: relationship between fermentation product yields of and catabolic enzyme activities in Clostridium thermocellum and Thermoanaerobium brockii. J Bacteriol. 1980;144:569–78.",{"doi":1744},"10.1128\u002Fjb.144.2.569-578.1980",{"id":1746,"createTime":1747,"updateTime":1748,"relativeEntities":1749,"slug":1750,"properties":1751,"entityType":138,"verifyStatus":139,"verifyTime":1748,"verifyNote":141,"languages":1762,"translateLanguages":18,"viewCount":19,"primaryUrl":1763,"fullTextUrl":18,"authors":1764,"publicationType":222,"publisherRelationship":1869,"citationCount":206,"citationInfo":1931,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":1933,"openAccess":18,"references":1934,"isForceReanalyzing":495},"43f4006b-3877-4e92-b4f0-b6362ef4c28d","2024-04-19T22:36:56.729+00:00","2025-02-26T11:25:37.550+00:00",[],"Enzymatic-debranching-is-a-key-determinant-of-the-xylan-degrading-activity-of-family-AA9-lytic-polysaccharide-monooxygenases",{"openalex":1752,"abstract":1754,"title":1756,"pm":1758,"doi":1760},{"VOID":1753},"W4313559575",{"EN":1755},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:sec>\n                \u003Cjats:title>Background\u003C\u002Fjats:title>\n                \u003Cjats:p>Previous studies have revealed that some Auxiliary Activity family 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) oxidize and degrade certain types of xylans when incubated with mixtures of xylan and cellulose. Here, we demonstrate that the xylanolytic activities of two xylan-active LPMOs, \u003Cjats:italic>Tt\u003C\u002Fjats:italic>LPMO9E and \u003Cjats:italic>Tt\u003C\u002Fjats:italic>LPMO9G from \u003Cjats:italic>Thermothielavioides terrestris\u003C\u002Fjats:italic>, strongly depend on the presence of xylan substitutions.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Results\u003C\u002Fjats:title>\n                \u003Cjats:p>Using mixtures of phosphoric acid-swollen cellulose (PASC) and wheat arabinoxylan (WAX), we show that removal of arabinosyl substitutions with a GH62 arabinofuranosidase resulted in better adsorption of xylan to cellulose, and enabled LPMO-catalyzed cleavage of this xylan. Furthermore, experiments with mixtures of PASC and arabinoglucuronoxylan from spruce showed that debranching of xylan with the GH62 arabinofuranosidase and a GH115 glucuronidase promoted LPMO activity. Analyses of mixtures with PASC and (non-arabinosylated) beechwood glucuronoxylan showed that GH115 action promoted LPMO activity also on this xylan. Remarkably, when WAX was incubated with Avicel instead of PASC in the presence of the GH62, both xylan and cellulose degradation by the LPMO9 were impaired, showing that the formation of cellulose–xylan complexes and their susceptibility to LPMO action also depend on the properties of the cellulose. These debranching effects not only relate to modulation of the cellulose–xylan interaction, which influences the conformation and rigidity of the xylan, but likely also affect the LPMO–xylan interaction, because debranching changes the architecture of the xylan surface.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Conclusions\u003C\u002Fjats:title>\n                \u003Cjats:p>Our results shed new light on xylanolytic LPMO9 activity and on the functional interplay and possible synergies between the members of complex lignocellulolytic enzyme cocktails. These findings will be relevant for the development of future lignocellulolytic cocktails and biomaterials.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>",{"EN":1757},"Enzymatic debranching is a key determinant of the xylan-degrading activity of family AA9 lytic polysaccharide monooxygenases",{"VOID":1759},"36604763",{"VOID":1761},"10.1186\u002Fs13068-022-02255-2",[143],"https:\u002F\u002Fbiotechnologyforbiofuels.biomedcentral.com\u002Farticles\u002F10.1186\u002Fs13068-022-02255-2",[1765,1782,1799,1818,1837,1852],{"id":1766,"sortIndex":19,"researcher":18,"roles":1767,"affiliations":1768,"properties":1777,"displayName":1779,"givenName":18,"familyName":18},"390b1160-ed79-4400-971d-5d48360f90b1",[],[1769],{"id":1770,"sortIndex":19,"affiliation":1771,"properties":18},"2eb777c3-23eb-42eb-9e77-61c0739bbe53",{"id":1770,"createTime":18,"updateTime":18,"relativeEntities":1772,"slug":18,"properties":1773,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":1776,"statistic":18},[],{"title":1774},{"EN":1775},"Division of Industrial Biotechnology, Department of Biology and Biological Engineering, Chalmers University of Technology, 412 96, Gothenburg, Sweden",[],{"title":1778,"openalex":1780},{"EN":1779},"Monika Tõlgo",{"VOID":1781},"A5024734399",{"id":1783,"sortIndex":112,"researcher":18,"roles":1784,"affiliations":1785,"properties":1794,"displayName":1796,"givenName":18,"familyName":18},"c9e324e8-cf77-47cb-9f3f-f0e674c0e442",[],[1786],{"id":1787,"sortIndex":19,"affiliation":1788,"properties":18},"9cc20ba7-6561-4282-9b6b-53be8d3b4fbe",{"id":1787,"createTime":18,"updateTime":18,"relativeEntities":1789,"slug":18,"properties":1790,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":1793,"statistic":18},[],{"title":1791},{"EN":1792},"Faculty of Chemistry, Biotechnology and Food Science, NMBU-Norwegian University of Life Sciences, 1433, Ås, Norway",[],{"title":1795,"openalex":1797},{"EN":1796},"Olav A. Hegnar",{"VOID":1798},"A5087466404",{"id":1800,"sortIndex":186,"researcher":18,"roles":1801,"affiliations":1802,"properties":1811,"displayName":1815,"givenName":18,"familyName":18},"670741db-b6e9-489c-bf29-477a2d6b9f77",[],[1803],{"id":1804,"sortIndex":19,"affiliation":1805,"properties":18},"6b47732f-e998-4b07-addb-444d8cfb1d66",{"id":1804,"createTime":18,"updateTime":18,"relativeEntities":1806,"slug":18,"properties":1807,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":1810,"statistic":18},[],{"title":1808},{"EN":1809},"Wallenberg Wood Science Centre, Chalmers University of Technology, 412 96, Gothenburg, Sweden",[],{"orcid":1812,"title":1814,"openalex":1816},{"VOID":1813},"https:\u002F\u002Forcid.org\u002F0000-0001-8386-2914",{"EN":1815},"Johan Larsbrink",{"VOID":1817},"A5021594300",{"id":1819,"sortIndex":206,"researcher":18,"roles":1820,"affiliations":1821,"properties":1830,"displayName":1834,"givenName":18,"familyName":18},"3a093e5a-d0a8-4358-b371-22cb581d47da",[],[1822],{"id":1823,"sortIndex":19,"affiliation":1824,"properties":18},"964e862f-dd58-470c-ace4-4b969003db09",{"id":1823,"createTime":18,"updateTime":18,"relativeEntities":1825,"slug":18,"properties":1826,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":1829,"statistic":18},[],{"title":1827},{"EN":1828},"Division of Glycoscience, Department of Chemistry, KTH Royal Institute of Technology, 106 91, Stockholm, Sweden",[],{"orcid":1831,"title":1833,"openalex":1835},{"VOID":1832},"https:\u002F\u002Forcid.org\u002F0000-0003-3572-7798",{"EN":1834},"Francisco Vilaplana",{"VOID":1836},"A5023486843",{"id":1838,"sortIndex":613,"researcher":18,"roles":1839,"affiliations":1840,"properties":1847,"displayName":1849,"givenName":18,"familyName":18},"e2c9c2e5-c6d6-485d-b5b4-0d7b49af9074",[],[1841],{"id":1787,"sortIndex":19,"affiliation":1842,"properties":18},{"id":1787,"createTime":18,"updateTime":18,"relativeEntities":1843,"slug":18,"properties":1844,"entityType":18,"verifyStatus":18,"verifyTime":18,"verifyNote":18,"languages":18,"translateLanguages":18,"viewCount":18,"url":18,"parentIds":1846,"statistic":18},[],{"title":1845},{"EN":1792},[],{"title":1848,"openalex":1850},{"EN":1849},"Vincent G. 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Mannanase hydrolysis of spruce galactoglucomannan focusing on the influence of acetylation on enzymatic mannan degradation. Biotechnol Biofuels. 2018;11(1):1–15.",{"doi":2120},"10.1186\u002Fs13068-018-1115-y",{"id":18,"text":2122,"url":18,"identifiers":2123},"Li X, Kouzounis D, Kabel MA, de Vries RP. GH10 and GH11 endoxylanases in Penicillium subrubescens: comparative characterization and synergy with GH51, GH54, GH62 α-L-arabinofuranosidases from the same fungus. N Biotechnol. 2022;70:84–92.",{"doi":2124},"10.1016\u002Fj.nbt.2022.05.004",{"id":18,"text":2126,"url":18,"identifiers":2127},"McKee LS, Sunner H, Anasontzis GE, Toriz G, Gatenholm P, Bulone V, et al. A GH115 α-glucuronidase from Schizophyllum commune contributes to the synergistic enzymatic deconstruction of softwood glucuronoarabinoxylan. 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In: Methods in enzymology. Academic Press; 1988. p. 19–25. https:\u002F\u002Fdoi.org\u002F10.1016\u002F0076-6879(88)60103-0",{"doi":2188},"10.1016\u002F0076-6879(88)60103-0",{"id":18,"text":2190,"url":18,"identifiers":2191},"Niedermeyer THJ, Strohalm M. mMass as a software tool for the annotation of cyclic peptide tandem mass spectra. PLoS ONE. 2012;7(9): e44913.",{"doi":2192},"10.1371\u002Fjournal.pone.0044913",{"id":2194,"createTime":2195,"updateTime":2196,"relativeEntities":2197,"slug":2198,"properties":2199,"entityType":138,"verifyStatus":139,"verifyTime":2196,"verifyNote":141,"languages":2210,"translateLanguages":18,"viewCount":19,"primaryUrl":2211,"fullTextUrl":18,"authors":2212,"publicationType":222,"publisherRelationship":2298,"citationCount":19,"citationInfo":2356,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":2358,"openAccess":18,"references":2359,"isForceReanalyzing":495},"4c25d9af-825b-4b0a-b4f1-e6a9f287ee27","2024-04-16T00:31:10.322+00:00","2025-02-26T09:06:42.646+00:00",[],"Enhanced-bacterial-cellulose-production-in-Komagataeibacter-sucrofermentans-impact-of-different-PQQ-dependent-dehydrogenase-knockouts-and-ethanol-supplementation",{"openalex":2200,"abstract":2202,"title":2204,"pm":2206,"doi":2208},{"VOID":2201},"W4392284029",{"EN":2203},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:sec>\n                \u003Cjats:title>Background\u003C\u002Fjats:title>\n                \u003Cjats:p>Bacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential. Despite many acetic acid bacteria (AAB) being BC overproducers, cost-effective production remains a challenge. The role of pyrroloquinoline quinone (PQQ)-dependent membrane dehydrogenases (mDH) is crucial in the metabolism of AAB since it links substrate incomplete oxidation in the periplasm to energy generation. Specifically, glucose oxidation to gluconic acid substantially lowers environmental pH and hinders BC production. Conversely, ethanol supplementation is known to enhance BC yields in \u003Cjats:italic>Komagataeibacter spp.\u003C\u002Fjats:italic> by promoting efficient glucose utilization.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Results\u003C\u002Fjats:title>\n                \u003Cjats:p>\u003Cjats:italic>K. sucrofermentans\u003C\u002Fjats:italic> ATCC 700178 was engineered, knocking out the four PQQ-mDHs, to assess their impact on BC production. The strain KS003, lacking PQQ-dependent glucose dehydrogenase (PQQ-GDH), did not produce gluconic acid and exhibited a 5.77-fold increase in BC production with glucose as the sole carbon source, and a 2.26-fold increase under optimal ethanol supplementation conditions. In contrast, the strain KS004, deficient in the PQQ-dependent alcohol dehydrogenase (PQQ-ADH), showed no significant change in BC yield in the single carbon source experiment but showed a restrained benefit from ethanol supplementation.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>\u003Cjats:sec>\n                \u003Cjats:title>Conclusions\u003C\u002Fjats:title>\n                \u003Cjats:p>The results underscore the critical influence of PQQ-GDH and PQQ-ADH and clarify the effect of ethanol supplementation on BC production in \u003Cjats:italic>K. sucrofermentans\u003C\u002Fjats:italic> ATCC 700178. This study provides a foundation for further metabolic pathway optimization, emphasizing the importance of diauxic ethanol metabolism for high BC production.\u003C\u002Fjats:p>\n              \u003C\u002Fjats:sec>",{"EN":2205},"Enhanced bacterial cellulose production in Komagataeibacter sucrofermentans: impact of different PQQ-dependent dehydrogenase knockouts and ethanol 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Biosci Biotechnol Biochem. 1996;60:575–9.",{"doi":2546},"10.1271\u002Fbbb.60.575",{"id":18,"text":2548,"url":18,"identifiers":2549},"Gomes RJ, Ida EI, Spinosa WA. Nutritional supplementation with amino acids on bacterial cellulose production by Komagataeibacter intermedius: effect analysis and application of response surface methodology. Appl Biochem Biotechnol. 2022;194:5017–36.",{"doi":2550},"10.1007\u002Fs12010-022-04013-4",{"id":2552,"createTime":2553,"updateTime":2554,"relativeEntities":2555,"slug":2556,"properties":2557,"entityType":138,"verifyStatus":139,"verifyTime":2571,"verifyNote":141,"languages":18,"translateLanguages":2572,"viewCount":19,"primaryUrl":2573,"fullTextUrl":18,"authors":2574,"publicationType":222,"publisherRelationship":2682,"citationCount":18,"citationInfo":18,"publishDate":2740,"publishYear":610,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":2741,"openAccess":18,"references":18,"isForceReanalyzing":495},"9ea6d35f-44e8-430f-a8a9-f90103a7540e","2024-04-07T09:01:13.349+00:00","2025-02-26T08:36:33.640+00:00",[],"Could-termites-be-hiding-a-goldmine-of-obscure-yet-promising-yeasts-for-energy-crisis-solutions-based-on-aromatic-wastes-A-critical-state-of-the-art-review",{"abstract":2558,"title":2561,"keywords":2564,"references":2567,"doi":2569},{"EN":2559,"VI":2560},"Biodiesel is a renewable fuel that can be produced from a range of organic and renewable feedstock including fresh or vegetable oils, animal fats, and oilseed plants. In recent years, the lignin-based aromatic wastes, such as various aromatic waste polymers from agriculture, or organic dye wastewater from textile industry, have attracted much attention in academia, which can be uniquely selected as a potential renewable feedstock for biodiesel product converted by yeast cell factory technology. This current investigation indicated that the highest percentage of lipid accumulation can be achieved as high as 47.25% by an oleaginous yeast strain, Meyerozyma caribbica SSA1654, isolated from a wood-feeding termite gut system, where its synthetic oil conversion ability can reach up to 0.08 (g\u002Fl\u002Fh) and the fatty acid composition in yeast cells represents over 95% of total fatty acids that are similar to that of vegetable oils. Clearly, the use of oleaginous yeasts, isolated from wood-feeding termites, for synthesizing lipids from aromatics is a clean, efficient, and competitive path to achieve \"a sustainable development\" towards biodiesel production. However, the lacking of potent oleaginous yeasts to transform lipids from various aromatics, and an unknown metabolic regulation mechanism presented in the natural oleaginous yeast cells are the fundamental challenge we have to face for a potential cell factory development. Under this scope, this review has proposed a novel concept and approach strategy in utilization of oleaginous yeasts as the cell factory to convert aromatic wastes to lipids as the substrate for biodiesel transformation. Therefore, screening robust oleaginous yeast strain(s) from wood-feeding termite gut system with a set of the desirable specific tolerance characteristics is essential. In addition, to reconstruct a desirable metabolic pathway\u002Fnetwork to maximize the lipid transformation and accumulation rate from the aromatic wastes with the applications of various “omics” technologies or a synthetic biology approach, where the work agenda will also include to analyze the genome characteristics, to develop a new base mutation gene editing technology, as well as to clarify the influence of the insertion position of aromatic compounds and other biosynthetic pathways in the industrial chassis genome on the expressional level and genome stability. With these unique designs running with a set of the advanced biotech approaches, a novel metabolic pathway using robust oleaginous yeast developed as a cell factory concept can be potentially constructed, integrated and optimized, suggesting that the hypothesis we proposed in utilizing aromatic wastes as a feedstock towards biodiesel product is technically promising and potentially applicable in the near future.","Nhiên liệu sinh học là một loại nhiên liệu tái tạo có thể được sản xuất từ nhiều nguồn nhiên liệu hữu cơ và tái tạo khác nhau bao gồm dầu thực vật, mỡ động vật và các loại cây có chứa dầu. Trong những năm gần đây, các loại chất thải thơm dựa trên lignin, như các loại polyme chất thải thơm từ nông nghiệp hoặc nước thải nhuộm hữu cơ từ ngành công nghiệp dệt may, đã thu hút nhiều sự chú ý trong giới học thuật, vì có thể được lựa chọn một cách độc đáo như một nguồn nguyên liệu tái tạo tiềm năng cho sản phẩm biodiesel được chuyển đổi thông qua công nghệ nhà máy tế bào nấm men. Nghiên cứu hiện tại chỉ ra rằng tỷ lệ tích lũy lipid cao nhất có thể đạt đến 47,25% bởi một chủng nấm men có dầu, Meyerozyma caribbica SSA1654, được phân lập từ hệ vi khuẩn của mối ăn gỗ, nơi khả năng chuyển đổi dầu tổng hợp của nó có thể đạt tới 0,08 (g\u002Fl\u002Fh) và thành phần axit béo trong tế bào nấm men đại diện cho hơn 95% tổng số axit béo tương tự như của dầu thực vật. Rõ ràng, việc sử dụng nấm men có dầu, được phân lập từ mối ăn gỗ, để tổng hợp lipid từ các chất thơm là một con đường sạch, hiệu quả và cạnh tranh nhằm đạt được \"phát triển bền vững\" hướng tới sản xuất biodiesel. Tuy nhiên, sự thiếu hụt các loại nấm men có dầu mạnh mẽ để chuyển đổi lipid từ nhiều loại chất thơm và cơ chế điều chỉnh chuyển hóa chưa được hiểu trong các tế bào nấm men tự nhiên là thách thức cơ bản mà chúng ta phải đối mặt trong việc phát triển nhà máy tế bào tiềm năng. Trong khuôn khổ này, bài tổng quan đã đề xuất một khái niệm và chiến lược tiếp cận mới về việc sử dụng nấm men có dầu như một nhà máy tế bào để chuyển đổi chất thải thơm thành lipid làm chất nền cho quá trình chuyển hóa biodiesel. Do đó, việc sàng lọc các chủng nấm men có dầu mạnh mẽ từ hệ vi khuẩn của mối ăn gỗ với một tập hợp các đặc tính chịu đựng mong muốn là điều cần thiết. Ngoài ra, việc tái cấu trúc mạng lưới\u002Fđường đi chuyển hóa mong muốn để tối đa hóa tỷ lệ chuyển hóa và tích lũy lipid từ các chất thải thơm bằng cách áp dụng các công nghệ \"omics\" khác nhau hoặc tiếp cận sinh học tổng hợp, nơi mà chương trình công việc cũng sẽ bao gồm phân tích các đặc điểm genome, phát triển một công nghệ chỉnh sửa gene đột biến cơ sở mới, cũng như làm rõ ảnh hưởng của vị trí chèn các hợp chất thơm và các con đường sinh tổng hợp khác trong genome khung công nghiệp đối với mức độ biểu hiện và ổn định genome. Với các thiết kế độc đáo này cùng với một tập hợp các phương pháp công nghệ sinh học tiên tiến, một con đường chuyển hóa mới sử dụng nấm men có dầu mạnh mẽ phát triển như một khái niệm nhà máy tế bào có thể sẽ được xây dựng, tích hợp và tối ưu hóa, cho thấy giả thuyết mà chúng tôi đề xuất trong việc sử dụng chất thải thơm làm nguyên liệu cho sản phẩm biodiesel có tiềm năng kỹ thuật hứa hẹn và khả năng áp dụng trong tương lai gần.",{"EN":2562,"VI":2563},"Could termites be hiding a goldmine of obscure yet promising yeasts for energy crisis solutions based on aromatic wastes? A critical state-of-the-art review","Liệu những con mối có đang ẩn chứa một kho báu của những giống nấm men tiềm năng để giải quyết khủng hoảng năng lượng từ các chất thải thơm? 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Identification and characterization of genes involved in the downstream degradation pathway of γ-hexachlorocyclohexane in Sphingomonas paucimobilis UT26. J Bacteriol. 2005;187:847–53.\nWatanabe T, Tsuda S, Nishimura H, Honda Y, Watanabe T. Characterization of a Δ12-fatty acid desaturase gene from Ceriporiopsis subvermispora, a selective lignin-degrading fungus. Appl Microbiol Biotechnol. 2010;87:215–24.\nYu X, Zeng J, Zheng Y, Chen S. Effect of lignocellulose degradation products on microbial biomass and lipid production by the oleaginous yeast Cryptococcus curvatus. Process Biochem. 2014;49:457–65.\nHu J, Zhang S, Xiao R, Jiang X, Wang Y, Sun Y, Lu P. Catalytic transfer hydrogenolysis of lignin into monophenols over platinum-rhenium supported on titanium dioxide using isopropanol as in situ hydrogen source. Bioresour Technol. 2019;279:228–33.\nSingh S, Pandey D, Saravanabhupathy S, Daverey A, Dutta K, Arunachalam K. Liquid wastes as a renewable feedstock for yeast biodiesel production: opportunities and challenges. Environ Res. 2022;207:112100.\nBhatia SK, Kim SH, Yoon JJ, Yang YH. Current status and strategies for second generation biofuel production using microbial systems. Energy Convers Manag. 2017;148:1142–56.\nAli SS, Sun J. Effective thermal pretreatment of water hyacinth (Eichhornia crassipes) for the enhancement of biomethanation: VIT® gene probe technology for microbial community analysis with special reference to methanogenic Archaea. J Environ Chem Eng. 2019;7:102853.\nAli SS, Mustafa AM, Kornaros M, Sun J, Khalil M, El-Shetehy M. Biodegradation of creosote-treated wood by two novel constructed microbial consortia for the enhancement of methane production. Bioresour Technol. 2021;323:124544.\nAli SS, Mustafa AM, Sun J. Wood-feeding termites as an obscure yet promising source of bacteria for biodegradation and detoxification of creosote-treated wood along with methane production enhancement. Bioresour Technol. 2021;338:125521.\nAli SS, Mustafa AM, Kornaros M, Manni A, Sun J, Khalil MA. Construction of novel microbial consortia CS-5 and BC-4 valued for the degradation of catalpa sawdust and chlorophenols simultaneously with enhancing methane production. Bioresour Technol. 2020;301:122720.\nAli SS, Kornaros M, Manni A, Sun J, El-Shanshoury AE, Kenawy ER, Khalil MA. Enhanced anaerobic digestion performance by two artificially constructed microbial consortia capable of woody biomass degradation and chlorophenols detoxification. J Hazard Mater. 2020;389:122076.\nAli SS, Jiao H, Mustafa AM, Koutra E, El-Sapagh S, Kornaros M, Elsamahy T, Khalil M, Bulgariu L, Sun J. Construction of a novel microbial consortium valued for the effective degradation and detoxification of creosote-treated sawdust along with enhanced methane production. J Hazard Mater. 2021;418:126091.\nKhot M, Ghosh D. Lipids of Rhodotorula mucilaginosa IIPL32 with biodiesel potential: oil yield, fatty acid profile, fuel properties. J Basic Microbiol. 2017;57:345–52.\nLing J, Tian Y, de Toledo RA, Shim H. Cost reduction for the lipid production from distillery and domestic mixed wastewater by Rhodosporidium toruloides via the reutilization of spent seed culture medium. Energy. 2017;136:135–41.\nHuang XF, Liu JN, Lu LJ, Peng KM, Yang GX, Liu J. Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides. Bioresour Technol. 2016;206:141–9.\nPatel A, Arora N, Pruthi V, Pruthi PA. Biological treatment of pulp and paper industry effluent by oleaginous yeast integrated with production of biodiesel as sustainable transportation fuel. J Clean Prod. 2017;142:2858–64.\nWare J, Megan W. Termites, social cockroaches. Reference module in life sciences. Cham: Elsevier; 2022.\nBrink DP, Ravi K, Lidén G, Gorwa-Grauslund MF. Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database. Appl Microbiol Biotechnol. 2019;103:3979–4002.\nMadzak C. Yarrowia lipolytica strains and their biotechnological applications: how natural biodiversity and metabolic engineering could contribute to cell factories improvement. J Fungi. 2021;7:548.\nGhogare R, Chen S, Xiong X. Metabolic engineering of oleaginous yeast Yarrowia lipolytica for overproduction of fatty acids. Front Microbiol. 2020;11:1717.\nVichaphund S, Wimuktiwan P, Soongprasit C, Sricharoenchaikul V, Atong D. Aromatic and aliphatic production of catalytic pyrolysis of lignin using ZSM-5\u002FAl-SBA-15 catalyst derived from high-calcium fly ash. Energy Rep. 2021;7:232–47.\nQiao K, Wasylenko TM, Zhou K, Xu P, Stephanopoulos G. Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism. 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the thermodynamic property is closer to gasoline, advanced biofuels (C ≥ 6) are appealing for replacing non-renewable fossil fuels using biosynthesis method that has presented a promising approach. Synthesizing advanced biofuels (C ≥ 6), in general, requires the expansion of carbon chains from three carbon atoms to more than six carbon atoms. Despite some specific biosynthesis pathways that have been developed in recent years, adequate summary is still lacking on how to obtain an effective metabolic pathway. Review of biosynthesis pathways for expanding carbon chains will be conducive to selecting, optimizing and discovering novel synthetic route to obtain new advanced biofuels. Herein, we first highlighted challenges on expanding carbon chains, followed by presentation of two biosynthesis strategies and review of three different types of biosynthesis pathways of carbon chain expansion for synthesizing advanced biofuels. Finally, we provided an outlook for the introduction of gene-editing technology in the development of new biosynthesis pathways of carbon chain expansion.\u003C\u002Fjats:p>",{"EN":2754},"Biosynthesis pathways of expanding carbon chains for producing advanced 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