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The CRISPR tool kit for genome editing and beyond. Nat Commun. 2018;9:1911.\nIshino S, Ishino Y. DNA polymerases as useful reagents for biotechnology - the history of developmental research in the field. Front Microbiol. 2014;5:465.\nIshino Y, Krupovic M, Forterre P. History of CRISPR-Cas from encounter with a mysterious repeated sequence to genome editing technology. J Bacteriol. 2018;200:e00580-e617.\nKurtzhals P, Nishimura E, Haahr H, Høeg-Jensen T, Johansson E, Madsen P, Sturis J, Kjeldsen T. Commemorating insulin’s centennial: engineering insulin pharmacology towards physiology. Trends Pharmacol Sci. 2021;42:620–39.\nRodriguez EL, Poddar S, Iftekhar S, Suh K, Woolfork AG, Ovbude S, Pekarek A, Walters M, Lott S, Hage DS. Affinity chromatography: a review of trends and developments over the past 50 years. J Chromatogr B Analyt Technol Biomed Life Sci. 2020;1157:122332.",{"EN":45},"Launching Advanced Biotechnology to elevate biotechnology research across disciplines, from biomedicine to agriculture",{"VOID":47},"10.1007\u002Fs44307-023-00001-9","PUBLICATION","VERIFIED","2025-02-09T20:14:09.109+00:00","Auto Verify","https:\u002F\u002Flink.springer.com\u002Farticle\u002F10.1007\u002Fs44307-023-00001-9",[54,71,94,107,120],{"id":55,"sortIndex":26,"researcher":18,"roles":56,"affiliations":58,"properties":68},"adb33c92-7fec-4b96-8e7a-a7a5965f52cf",[57],"AUTHOR",[59],{"id":18,"sortIndex":19,"affiliation":60,"properties":18},{"id":61,"createTime":62,"updateTime":62,"relativeEntities":63,"slug":18,"properties":64,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"be55fe18-c459-4862-98bc-f88f90ca6098","2023-12-28T08:50:00.275+00:00",[],{"title":65},{"VI":66},"State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, China","AFFILIATION",{"title":69},{"VI":70},"Jianhua Yang",{"id":72,"sortIndex":73,"researcher":18,"roles":74,"affiliations":75,"properties":91},"56e7aa09-b37a-4b70-adb5-e890af922915",4,[57],[76,86],{"id":77,"sortIndex":26,"affiliation":78,"properties":85},"0c39f18a-836c-43de-9c88-2e0d579754dd",{"id":79,"createTime":80,"updateTime":80,"relativeEntities":81,"slug":18,"properties":82,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"8b9ce231-eed1-4b9b-a272-8f0d7a64b6d6","2023-12-07T14:35:39.994+00:00",[],{"title":83},{"VI":84},"Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (CAFS), Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Qingdao, China",{},{"id":18,"sortIndex":19,"affiliation":87,"properties":18},{"id":61,"createTime":62,"updateTime":62,"relativeEntities":88,"slug":18,"properties":89,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":90},{"VI":66},{"title":92},{"VI":93},"Songlin Chen",{"id":95,"sortIndex":96,"researcher":18,"roles":97,"affiliations":98,"properties":104},"441304ba-743a-4773-9757-f4f7776a8eda",3,[57],[99],{"id":18,"sortIndex":19,"affiliation":100,"properties":18},{"id":61,"createTime":62,"updateTime":62,"relativeEntities":101,"slug":18,"properties":102,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":103},{"VI":66},{"title":105},{"VI":106},"Lianghu Qu",{"id":108,"sortIndex":109,"researcher":18,"roles":110,"affiliations":111,"properties":117},"26458fa5-8e69-463f-b78f-c9e83dc8425f",2,[57],[112],{"id":18,"sortIndex":19,"affiliation":113,"properties":18},{"id":61,"createTime":62,"updateTime":62,"relativeEntities":114,"slug":18,"properties":115,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":116},{"VI":66},{"title":118},{"VI":119},"Jianguo He",{"id":121,"sortIndex":19,"researcher":18,"roles":122,"affiliations":123,"properties":129},"7b9b1c43-adc8-4432-aa21-d89e6729ee36",[57],[124],{"id":18,"sortIndex":19,"affiliation":125,"properties":18},{"id":61,"createTime":62,"updateTime":62,"relativeEntities":126,"slug":18,"properties":127,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":128},{"VI":66},{"title":130},{"VI":131},"Shi Xiao","ARTICLE",{"url":52,"publisher":134,"properties":147},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":135,"slug":10,"properties":136,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":139,"manageAffiliations":140,"indexDatabases":141,"url":23,"thumbnailPath":18,"statistic":142,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":137,"title":138},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":143,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":144,"totalCitation":19,"totalCitationByYear":145,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":146,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},{"volume":148,"pages":150},{"VOID":149},"1",{"VOID":151},"1-2","2023-10-26",2023,false,{"id":156,"createTime":157,"updateTime":158,"relativeEntities":159,"slug":160,"properties":161,"entityType":48,"verifyStatus":49,"verifyTime":175,"verifyNote":51,"syncStatus":17,"languages":18,"translateLanguages":176,"viewCount":19,"primaryUrl":178,"fullTextUrl":18,"authors":179,"publicationType":132,"publisherRelationship":359,"citationCount":18,"citationInfo":18,"publishDate":373,"publishYear":374,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":18,"isForceReanalyzing":154},"e6e6be57-e189-41e0-855c-4355d446a105","2024-04-06T18:33:58.536+00:00","2025-02-19T18:33:06.547+00:00",[],"Development-of-a-CRISPR-Cas12a-based-assay-for-the-detection-of-swine-enteric-coronaviruses-in-pig-herds-in-China",{"references":162,"keywords":164,"abstract":167,"title":170,"doi":173},{"VOID":163},"Bandyopadhyay 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Novel human coronavirus (SARS-CoV-2): A lesson from animal coronaviruses. Vet Microbiol. 2020;244: 108693.\nDomingo E, Garcia-Crespo C, Lobo-Vega R, Perales C. Mutation rates, mutation frequencies, and proofreading-repair activities in RNA virus genetics. Viruses. 2021;13:1882.\nEdwards CE, Yount BL, Graham RL, Leist SR, Hou YJ, Dinnon KR, Sims AC, Swanstrom J, Gully K, Scobey TD, Cooley MR, Currie CG, Randell SH, Baric RS. Swine acute diarrhea syndrome coronavirus replication in primary human cells reveals potential susceptibility to infection. Proc Natl Acad Sci U S A. 2020;117:26915–25.\nGong L, Li J, Zhou Q, Xu Z, Chen L, Zhang Y, Xue C, Wen Z, Cao Y. A New Bat-HKU2-like Coronavirus in Swine, China, 2017. Emerg Infect Dis. 2017;23:1607–9.\nGong H, Wu Y, Zeng R, Zeng Y, Liu X, Tang D. CRISPR\u002FCas12a-mediated liposome-amplified strategy for the photoelectrochemical detection of nucleic acid. Chem Commun (camb). 2021;57:8977–80.\nGootenberg JS, Abudayyeh OO, Kellner MJ, Joung J, Collins JJ, Zhang F. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6. Science. 2018;360(6387):439–44.\nHaake C, Cook S, Pusterla N, Murphy B. Coronavirus infections in companion animals: virology, epidemiology, clinical and pathologic features. Viruses. 2020;12(9):1023.\nHao J, Xue C, He L, Wang Y, Cao Y. Bioinformatics insight into the spike glycoprotein gene of field porcine epidemic diarrhea strains during 2011–2013 in Guangdong. China Virus Genes. 2014;49:58–67.\nHe WT, Ji X, He W, Dellicour S, Wang S, Li G, Zhang L, Gilbert M, Zhu H, Xing G, Veit M, Huang Z, Han GZ, Huang Y, Suchard MA, Baele G, Lemey P, Su S. Genomic epidemiology, evolution, and transmission dynamics of porcine deltacoronavirus. Mol Biol Evol. 2020;37:2641–54.\nJung K, Saif LJ, Wang Q. Porcine epidemic diarrhea virus (PEDV): An update on etiology, transmission, pathogenesis, and prevention and control. Virus Res. 2020;286: 198045.\nKim SH, Kim IJ, Pyo HM, Tark DS, Song JY, Hyun BH. Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus. J Virol Methods. 2007;146:172–7.\nLednicky JA, Tagliamonte MS, White SK, Elbadry MA, Alam MM, Stephenson CJ, Bonny TS, Loeb JC, Telisma T, Chavannes S, Ostrov DA, Mavian C, Beau DRV, Salemi M, Morris JJ. Independent infections of porcine deltacoronavirus among Haitian children. Nature. 2021;600:133–7.\nLi P, Ren X. Reverse transcription loop-mediated isothermal amplification for rapid detection of transmissible gastroenteritis virus. Curr Microbiol. 2011;62:1074–80.\nLi Y, Zheng F, Fan B, Muhammad HM, Zou Y, Jiang P. Development of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virus. Can J Microbiol. 2015;61:811–7.\nLi S, Cheng Q, Wang J, Li X, Zhang Z, Gao S, Cao R, Zhao G, Wang J. CRISPR-Cas12a-assisted nucleic acid detection. Cell Discov. 2018;4:20.\nLi C, Lu H, Geng C, Yang K, Liu W, Liu Z, Yuan F, Gao T, Wang S, Wen P, Song H, Tian Y, Zhou D. Epidemic and evolutionary characteristics of swine enteric viruses in south-central China from 2018 to 2021. Viruses. 2022;14:1420.\nLi M, Pan Y, Xi Y, Wang M, Zeng Q. Insights and progress on epidemic characteristics, genotyping, and preventive measures of PEDV in China: A review. Microb Pathog. 2023;181: 106185.\nLiang Y, Lin H, Zou L, Zhao J, Li B, Wang H, Lu J, Sun J, Yang X, Deng X, Tang S. CRISPR-Cas12a-Based Detection for the Major SARS-CoV-2 Variants of Concern. Microbiol Spectr. 2021;9: e0101721.\nLin H, Zhou H, Gao L, Li B, He K, Fan H. Development and application of an indirect ELISA for the detection of antibodies to porcine epidemic diarrhea virus based on a recombinant spike protein. BMC Vet Res. 2018;14:243.\nLiu Q, Wang HY. Porcine enteric coronaviruses: an updated overview of the pathogenesis, prevalence, and diagnosis. Vet Res Commun. 2021;45:75–86.\nLiu Y, Liang QZ, Lu W, Yang YL, Chen R, Huang YW, Wang B. A comparative analysis of coronavirus nucleocapsid (N) proteins reveals the SADS-CoV N protein antagonizes IFN-beta production by inducing ubiquitination of RIG-I. Front Immunol. 2021;12: 688758.\nLu R, Zhao X, Li J, Niu P, Yang B, Wu H, Wang W, Song H, Huang B, Zhu N, Bi Y, Ma X, Zhan F, Wang L, Hu T, Zhou H, Hu Z, Zhou W, Zhao L, Chen J, Meng Y, Wang J, Lin Y, Yuan J, Xie Z, Ma J, Liu WJ, Wang D, Xu W, Holmes EC, Gao GF, Wu G, Chen W, Shi W, Tan W. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding. Lancet. 2020;395:565–74.\nMa Y, Zhang Y, Liang X, Lou F, Oglesbee M, Krakowka S. Origin, evolution, and virulence of porcine deltacoronaviruses in the United States. mBio. 2015;6(2):e00064.\nMackay IM, Arden KE, Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 2002;30:1292–305.\nManghwar H, Lindsey K, Zhang X, Jin S. CRISPR\u002FCas system: recent advances and future prospects for genome editing. Trends Plant Sci. 2019;24:1102–25.\nMao Z, Chen R, Wang X, Zhou Z, Peng Y, Li S, Han D, Li S, Wang Y, Han T, Liang J, Ren S, Gao Z. CRISPR\u002FCas12a-based technology: A powerful tool for biosensing in food safety. Trends Food Sci Technol. 2022;122:211–22.\nMarraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet. 2010;11:181–90.\nPan Z, Lu J, Wang N, He WT, Zhang L, Zhao W, Su S. Development of a TaqMan-probe-based multiplex real-time PCR for the simultaneous detection of emerging and reemerging swine coronaviruses. Virulence. 2020;11:707–18.\nPaul B, Montoya G. 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Virol J. 2018;15:102.\nXiong D, Dai W, Gong J, Li G, Liu N, Wu W, Pan J, Chen C, Jiao Y, Deng H, Ye J, Zhang X, Huang H, Li Q, Xue L, Zhang X, Tang G. Rapid detection of SARS-CoV-2 with CRISPR-Cas12a. PLoS Biol. 2020;18: e3000978.\nXu Z, Zhong H, Zhou Q, Du Y, Chen L, Zhang Y, Xue C, Cao Y. A Highly Pathogenic Strain of Porcine Deltacoronavirus Caused Watery Diarrhea in Newborn Piglets. Virol Sin. 2018;33:131–41.\nXu Z, Zhang Y, Gong L, Huang L, Lin Y, Qin J, Du Y, Zhou Q, Xue C, Cao Y. Isolation and characterization of a highly pathogenic strain of Porcine enteric alphacoronavirus causing watery diarrhoea and high mortality in newborn piglets. Transbound Emerg Dis. 2019;66:119–30.\nYan Q, Liu X, Sun Y, Zeng W, Li Y, Zhao F, Wu K, Fan S, Zhao M, Chen J, Yi L. Swine enteric coronavirus: diverse pathogen-host interactions. Int J Mol Sci. 2022;23:3953.\nYang YL, Qin P, Wang B, Liu Y, Xu GH, Peng L, Zhou J, Zhu SJ, Huang YW. Broad cross-species infection of cultured cells by bat HKU2-related swine acute diarrhea syndrome coronavirus and identification of its replication in murine dendritic cells in vivo highlight its potential for diverse interspecies transmission. J Virol. 2019;93:e01448-e1519.\nYang YL, Yu JQ, Huang YW. Swine enteric alphacoronavirus (swine acute diarrhea syndrome coronavirus): An update three years after its discovery. Virus Res. 2020;285: 198024.\nZetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system. Cell. 2015;163:759–71.\nZhai SL, Wei WK, Li XP, Wen XH, Zhou X, Zhang H, Lv DH, Li F, Wang D. Occurrence and sequence analysis of porcine deltacoronaviruses in southern China. Virol J. 2016;13:136.\nZhai X, Kong N, Zhang Y, Song Y, Qin W, Yang X, Ye C, Ye M, Tong W, Liu C, Zheng H, Yu H, Zhang W, Yang X, Zhang G, Tong G, Shan T. N protein of PEDV plays chess game with host proteins by selective autophagy. Autophagy. 2023;19:2338–52.\nZhang J. Porcine deltacoronavirus: Overview of infection dynamics, diagnostic methods, prevalence and genetic evolution. Virus Res. 2016;226:71–84.\nZhang H, Zou C, Peng O, Ashraf U, Xu Q, Gong L, Fan B, Zhang Y, Xu Z, Xue C, Wei X, Zhou Q, Tian X, Shen H, Li B, Zhang X, Cao Y. Global dynamics of porcine enteric coronavirus PEDV epidemiology, evolution, and transmission. Mol Biol Evol. 2023;40(3):msad052.",{"EN":165,"VI":166},"","Virus tiêu chảy dịch tả lợn, Virus tiêu chảy truyền nhiễm, Virus delta coronavirus ở lợn, Coronavirus hội chứng tiêu chảy cấp lợn, Phương pháp phát hiện, CRISPR-Cas12a, Chăn nuôi lợn",{"EN":168,"VI":169},"Porcine epidemic diarrhea virus (PEDV), Transmissible gastroenteritis virus (TGEV), Porcine deltacoronavirus (PDCoV) and Swine acute diarrhea syndrome coronavirus (SADS-CoV) rank among the most frequently encountered swine enteric coronaviruses (SECoVs), leading to substantial economic losses to the swine industry. The availability of a rapid and highly sensitive detection method proves beneficial for the monitoring and surveillance of SECoVs. Based on the N genes of four distinct SECoVs, a novel detection method was developed in this study by combining recombinant enzyme polymerase isothermal amplification (RPA) with clustered regularly interspaced short palindromic repeats (CRISPR)-associated proteins (Cas) 12a. Results showed that the cut-off value of CRISPR-Cas12a assay for SADS-CoV, PEDV, PDCoV and TGEV was 2.19 × 104 Relative Fluorescence Units (RFU), 1.57 × 104 RFU, 3.07 × 104 RFU and 1.64 × 104 RFU, respectively. The coefficient of variation (CV) of within and between runs by CRISPR-Cas12a assay for 6 clinical diarrhea samples were both less than 10%. The CRISPR-Cas12a assay demonstrated high specificity for TGEV, PEDV, PDCoV, and SADS-CoV with no cross-reactivity to other common swine viruses. This method also exhibited a low limit of detection of 2 copies for each virus. Additionally, the results demonstrated a perfect agreement (100%) between the CRISPR-Cas12a assay and the RT-qPCR assay. Finally, a total of 494 pig samples from the field tested by CRISPR-Cas12a assay showed that positive rate for SADS-CoV, TGEV, PDCoV and PEDV was 0, 0, 1.2% and 48.6%, respectively. The results suggested the great potential of CRISPR-Cas12a assay to detect SECoVs in the field.","Virus tiêu chảy dịch tả lợn (PEDV), virus tiêu chảy truyền nhiễm (TGEV), virus delta coronavirus ở lợn (PDCoV) và coronavirus hội chứng tiêu chảy cấp lợn (SADS-CoV) là những loại coronavirus đường ruột ở lợn (SECoVs) thường gặp nhất, gây ra tổn thất kinh tế đáng kể cho ngành chăn nuôi lợn. Việc có một phương pháp phát hiện nhanh chóng và nhạy cảm cao là rất có lợi cho việc giám sát và kiểm tra SECoVs. Dựa trên các gen N của bốn loại SECoVs khác nhau, một phương pháp phát hiện mới đã được phát triển trong nghiên cứu này bằng cách kết hợp khuếch đại enzyme polymerase tái tổ hợp (RPA) với các protein liên quan đến các đoạn lặp lại ngắn phân tán có cụm (CRISPR) - Cas 12a. Kết quả cho thấy giá trị ngưỡng của xét nghiệm CRISPR-Cas12a cho SADS-CoV, PEDV, PDCoV và TGEV lần lượt là 2,19×104 Đơn vị phát quang tương đối (RFU), 1,57×104 RFU, 3,07×104 RFU và 1,64×104 RFU. Hệ số biến thiên (CV) của các mẫu tiêu chảy lâm sàng với xét nghiệm CRISPR-Cas12a đều nhỏ hơn 10%. Xét nghiệm CRISPR-Cas12a thể hiện tính chuyên biệt cao đối với TGEV, PEDV, PDCoV và SADS-CoV, không có sự phản ứng chéo với các virus lợn thông thường khác. Phương pháp này cũng cho thấy ngưỡng phát hiện thấp là 2 bản sao cho mỗi virus. Thêm vào đó, kết quả cho thấy sự đồng nhất hoàn hảo (100%) giữa xét nghiệm CRISPR-Cas12a và xét nghiệm RT-qPCR. Cuối cùng, tổng cộng 494 mẫu lợn từ thực địa được thử nghiệm bằng xét nghiệm CRISPR-Cas12a cho thấy tỷ lệ dương tính với SADS-CoV, TGEV, PDCoV và PEDV lần lượt là 0, 0, 1,2% và 48,6%. Kết quả cho thấy tiềm năng lớn của xét nghiệm CRISPR-Cas12a trong việc phát hiện SECoVs tại thực địa.",{"EN":171,"VI":172},"Development of a CRISPR-Cas12a based assay for the detection of swine enteric coronaviruses in pig herds in China","Phát triển phương pháp xét nghiệm dựa trên CRISPR-Cas12a cho việc phát hiện coronavirus đường ruột ở lợn trong các trang trại lợn ở Trung 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Additionally, we highlight recent advancements in ribosome profiling that enable the transition from bulk to low-input and single-cell applications. Single-cell ribosome profiling has emerged as a crucial tool for exploring translation heterogeneity within specific cell populations. However, the challenges of capturing mRNAs efficiently and the sparse nature of footprint reads in single-cell ribosome profiling present ongoing obstacles. The need to refine ribosome profiling techniques remains, especially when used at the single-cell level.","Phân tích ribosome đã cách mạng hóa hiểu biết của chúng ta về sự điều chỉnh biểu hiện gen bằng cách cung cấp một cái nhìn tổng quan về dịch mã toàn cầu trong điều kiện sống. Kỹ thuật mạnh mẽ này cho phép điều tra động thái của sự khởi đầu dịch mã, kéo dài và kết thúc, và đã cung cấp những hiểu biết về quy trình tổng hợp protein trong các điều kiện khác nhau. Mặc dù việc áp dụng rộng rãi, những thách thức vẫn tồn tại trong việc thu được dữ liệu phân tích ribosome chất lượng cao. Trong bài tổng quan này, chúng tôi thảo luận về các nguyên tắc cơ bản của phân tích ribosome và các phương pháp liên quan, bao gồm phân tích ribosome chọn lọc và phân tích phức hợp dịch mã. Chúng tôi cũng đi sâu vào kiểm soát chất lượng để đánh giá độ tin cậy của các bộ dữ liệu phân tích ribosome, và những nỗ lực nhằm cải thiện chất lượng dữ liệu bằng cách điều chỉnh các quy trình tiêu chuẩn. Thêm vào đó, chúng tôi nhấn mạnh những tiến bộ gần đây trong phân tích ribosome cho phép chuyển đổi từ khối lượng lớn sang các ứng dụng đầu vào thấp và tế bào đơn. Phân tích ribosome tế bào đơn đã nổi lên như một công cụ quan trọng để khám phá sự khác biệt trong dịch mã trong các quần thể tế bào cụ thể. Tuy nhiên, những thách thức trong việc nắm bắt mRNA một cách hiệu quả và bản chất thưa thớt của các biến thể đọc trong phân tích ribosome tế bào đơn vẫn là những rào cản chưa được giải quyết. Nhu cầu cải tiến kỹ thuật phân tích ribosome vẫn còn, đặc biệt khi được sử dụng ở cấp độ tế bào đơn.",{"EN":388,"VI":389},"Principles, challenges, and advances in ribosome profiling: from bulk to low-input and single-cell analysis","Nguyên tắc, thách thức và tiến bộ trong phân tích ribosome: từ khối lượng lớn tới đầu vào thấp và phân tích tế bào đơn",{"VOID":391},"10.1007\u002Fs44307-023-00006-4",{"VI":393},"ribosome profiling, dịch mã, sự điều chỉnh biểu hiện gen, phân tích tế bào đơn, chất lượng dữ liệu","2025-01-03T03:16:51.459+00:00",[177],"https:\u002F\u002Flink.springer.com\u002Farticle\u002F10.1007\u002Fs44307-023-00006-4",[398,425],{"id":399,"sortIndex":19,"researcher":18,"roles":400,"affiliations":401,"properties":422},"db64858b-6f1a-4487-a746-743e74506708",[57],[402,412],{"id":403,"sortIndex":26,"affiliation":404,"properties":411},"731d0546-9310-43f3-bbd1-91ce07b7307f",{"id":405,"createTime":406,"updateTime":406,"relativeEntities":407,"slug":18,"properties":408,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"cc0f1b29-2f7c-468c-846d-b759df6d9299","2024-02-19T02:02:22.863+00:00",[],{"title":409},{"VI":410},"Liangzhu Laboratory, School of Medicine, Zhejiang University, Hangzhou, China",{},{"id":18,"sortIndex":19,"affiliation":413,"properties":18},{"id":414,"createTime":415,"updateTime":416,"relativeEntities":417,"slug":418,"properties":419,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"162eca9d-dbea-4416-98ca-f51f8a5a6305","2023-12-26T01:43:28.294+00:00","2025-02-04T07:23:39.175+00:00",[],"Bone-Marrow-Transplantation-Center-The-First-Affiliated-Hospital-School-of-Medicine-Zhejiang-University-Hangzhou-China",{"title":420},{"VI":421},"Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China",{"title":423},{"VI":424},"Qiuyi Wang",{"id":426,"sortIndex":26,"researcher":18,"roles":427,"affiliations":428,"properties":441},"ff99e63d-0c38-45b9-98f1-f9901679fa69",[57],[429,434],{"id":18,"sortIndex":19,"affiliation":430,"properties":18},{"id":414,"createTime":415,"updateTime":416,"relativeEntities":431,"slug":418,"properties":432,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":433},{"VI":421},{"id":435,"sortIndex":26,"affiliation":436,"properties":440},"7314180d-82cf-4363-b0f7-9d0bdac91d49",{"id":405,"createTime":406,"updateTime":406,"relativeEntities":437,"slug":18,"properties":438,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":439},{"VI":410},{},{"title":442},{"VI":443},"Yuanhui Mao",{"url":396,"publisher":445,"properties":458},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":446,"slug":10,"properties":447,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":450,"manageAffiliations":451,"indexDatabases":452,"url":23,"thumbnailPath":18,"statistic":453,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":448,"title":449},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":454,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":455,"totalCitation":19,"totalCitationByYear":456,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":457,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},{"volume":459,"pages":460},{"VOID":149},{"VOID":461},"1-14","2023-12-01",{"id":464,"createTime":465,"updateTime":466,"relativeEntities":467,"slug":468,"properties":469,"entityType":48,"verifyStatus":49,"verifyTime":482,"verifyNote":51,"syncStatus":17,"languages":18,"translateLanguages":483,"viewCount":19,"primaryUrl":484,"fullTextUrl":18,"authors":485,"publicationType":132,"publisherRelationship":514,"citationCount":18,"citationInfo":18,"publishDate":528,"publishYear":374,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":18,"isForceReanalyzing":154},"3eab0538-029d-4e16-9601-9161c079bbe4","2024-04-06T19:22:09.698+00:00","2025-02-19T18:31:08.478+00:00",[],"Technologies-for-studying-phase-separated-biomolecular-condensates",{"references":470,"keywords":472,"abstract":474,"title":477,"doi":480},{"VOID":471},"Alberti S, et al. 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APL Bioengineering. 2023c;7:021502.\nZhao EM, et al. Light-based control of metabolic flux through assembly of synthetic organelles. Nat Chem Biol. 2019;15:589–97.\nZhou W, Mohr L, Maciejowski J, Kranzusch PJ. cGAS phase separation inhibits TREX1-mediated DNA degradation and enhances cytosolic DNA sensing. Mol Cell. 2021;81:739-755 e737.\nZhou XM, et al. Mutations linked to neurological disease enhance self-association of low-complexity protein sequences. Science. 2022;377(6601):eabn5582.\nZhu S, et al. Liquid-liquid phase separation of RBGD2\u002F4 is required for heat stress resistance in Arabidopsis. Dev Cell. 2022;57:583–597.e586.\nZhuang YR, et al. Circadian clocks are modulated by compartmentalized oscillating translation. Cell. 2023;186(15):3245–60.e23.",{"EN":165,"VI":473},"ngưng tụ phân tử sinh học, phân pha lỏng-lỏng, bào quan không có màng, công nghệ sinh học, sinh học tổng hợp",{"EN":475,"VI":476},"Biomolecular condensates, also referred to as membrane-less organelles, function as fundamental organizational units within cells. These structures primarily form through liquid–liquid phase separation, a process in which proteins and nucleic acids segregate from the surrounding milieu to assemble into micron-scale structures. By concentrating functionally related proteins and nucleic acids, these biomolecular condensates regulate a myriad of essential cellular processes. To study these significant and intricate organelles, a range of technologies have been either adapted or developed. In this review, we provide an overview of the most utilized technologies in this rapidly evolving field. These include methods used to identify new condensates, explore their components, investigate their properties and spatiotemporal regulation, and understand the organizational principles governing these condensates. We also discuss potential challenges and review current advancements in applying the principles of biomolecular condensates to the development of new technologies, such as those in synthetic biology.","Các ngưng tụ phân tử sinh học, còn được gọi là bào quan không có màng, đóng vai trò là đơn vị tổ chức cơ bản trong tế bào. Các cấu trúc này chủ yếu hình thành thông qua quá trình phân pha lỏng-lỏng, trong đó protein và axit nucleic tách biệt khỏi môi trường xung quanh để lắp ráp thành các cấu trúc ở quy mô micromet. Bằng cách tập trung các protein và axit nucleic có chức năng liên quan, các ngưng tụ phân tử sinh học này điều chỉnh vô số quá trình tế bào thiết yếu. Để nghiên cứu những bào quan quan trọng và phức tạp này, một loạt các công nghệ đã được điều chỉnh hoặc phát triển. Trong bài đánh giá này, chúng tôi cung cấp cái nhìn tổng quan về các công nghệ được sử dụng nhiều nhất trong lĩnh vực đang phát triển nhanh chóng này. Những công nghệ này bao gồm các phương pháp được sử dụng để xác định các ngưng tụ mới, khám phá các thành phần của chúng, điều tra các thuộc tính và quy định không gian-thời gian của chúng, và hiểu các nguyên tắc tổ chức điều khiển các ngưng tụ này. Chúng tôi cũng thảo luận về những thách thức tiềm năng và đánh giá những tiến bộ hiện tại trong việc áp dụng các nguyên tắc của các ngưng tụ phân tử sinh học vào việc phát triển các công nghệ mới, chẳng hạn như trong sinh học tổng hợp.",{"EN":478,"VI":479},"Technologies for studying phase-separated biomolecular condensates","Công nghệ nghiên cứu các ngưng tụ phân tử sinh học phân pha",{"VOID":481},"10.1007\u002Fs44307-024-00020-0","2025-01-05T17:19:36.228+00:00",[177],"https:\u002F\u002Flink.springer.com\u002Farticle\u002F10.1007\u002Fs44307-024-00020-0",[486,502],{"id":487,"sortIndex":19,"researcher":18,"roles":488,"affiliations":489,"properties":499},"2887ae94-c160-49d3-8483-3d28118b9c26",[57],[490],{"id":18,"sortIndex":19,"affiliation":491,"properties":18},{"id":492,"createTime":493,"updateTime":493,"relativeEntities":494,"slug":495,"properties":496,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"2602c1af-61a5-41ab-8275-1260ec8e6a99","2024-04-06T19:22:09.714+00:00",[],"Guangdong-Provincial-Key-Laboratory-of-Pharmaceutical-Functional-Genes-MOE-Key-Laboratory-of-Gene-Function-and-Regulation-State-Key-Laboratory-of-Biocontrol-School-of-Life-Sciences-Sun-Yat-Sen-University-GuangZhou-China",{"title":497},{"VI":498},"Guangdong Provincial Key Laboratory of Pharmaceutical Functional Genes, MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, GuangZhou, China",{"title":500},{"VI":501},"Boyuan Deng",{"id":503,"sortIndex":26,"researcher":18,"roles":504,"affiliations":505,"properties":511},"d27a20fa-7e94-49ce-badb-303a24fc5f43",[57],[506],{"id":18,"sortIndex":19,"affiliation":507,"properties":18},{"id":492,"createTime":493,"updateTime":493,"relativeEntities":508,"slug":495,"properties":509,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":510},{"VI":498},{"title":512},{"VI":513},"Gang Wan",{"url":18,"publisher":515,"properties":18},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":516,"slug":10,"properties":517,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":520,"manageAffiliations":521,"indexDatabases":522,"url":23,"thumbnailPath":18,"statistic":523,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":518,"title":519},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":524,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":525,"totalCitation":19,"totalCitationByYear":526,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":527,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},"2024-03-07",{"id":530,"createTime":531,"updateTime":532,"relativeEntities":533,"slug":534,"properties":535,"entityType":48,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":547,"translateLanguages":549,"viewCount":19,"primaryUrl":550,"fullTextUrl":18,"authors":551,"publicationType":132,"publisherRelationship":618,"citationCount":19,"citationInfo":632,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":634,"isForceReanalyzing":154},"d2944a6a-1642-4977-bf80-bd24618f0571","2024-04-14T09:58:33.603+00:00","2025-02-19T18:30:11.129+00:00",[],"Applications-of-mass-spectrometry-imaging-in-botanical-research",{"keywords":536,"openalex":537,"abstract":539,"title":542,"doi":545},{"VI":165},{"VOID":538},"W4391845987",{"EN":540,"VI":541},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>Mass spectrometry imaging (MSI) serves as a valuable tool enabling researchers to scrutinize various compounds, peptides, and proteins within a sample, providing detailed insights at both elemental and molecular levels. This innovative technology transforms information obtained from a mass spectrometer— encompassing ionic strength, mass-to-charge ratio, and ionized molecule coordinates—within a defined region into a pixel-based model. Consequently, it reconstructs the spatial distribution of ions, allowing for a comprehensive understanding of molecular landscapes. The significance of MSI lies in its ability to offer multiple advantages, including straightforward sample preparation and remarkable sensitivity, all achieved without the necessity for labeling. Particularly in the realm of plant biology, MSI finds frequent application in examining the distribution of target metabolites and other components within plant tissues. This review delves into the fundamental principles, distinguishing features, merits, and applications of three prominent MSI technologies. Furthermore, we aim to assist readers in navigating the utilization of MSI in their plant biology research by discussing primary challenges, proposing potential solutions, and elucidating future prospects associated with this cutting-edge technology.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Phân tích quang phổ khối hình ảnh (MSI) là một công cụ quý giá cho phép các nhà nghiên cứu khảo sát nhiều hợp chất, peptide và protein trong một mẫu vật, cung cấp những hiểu biết chi tiết ở cả cấp độ nguyên tố và phân tử. Công nghệ đổi mới này chuyển đổi thông tin thu được từ một thiết bị quang phổ khối—bao gồm độ mạnh ion, tỉ lệ khối lượng trên điện tích và tọa độ phân tử ion hóa—trong một khu vực xác định thành một mô hình dựa trên pixel. Do đó, nó tái tạo cấu trúc phân bố không gian của các ion, cho phép hiểu biết toàn diện về cảnh quan phân tử. Ý nghĩa của MSI nằm ở khả năng cung cấp nhiều lợi ích, bao gồm chuẩn bị mẫu đơn giản và độ nhạy đáng kinh ngạc, tất cả đều đạt được mà không cần đánh dấu. Đặc biệt trong lĩnh vực sinh học thực vật, MSI thường được áp dụng để nghiên cứu sự phân bố của các chuyển hoá mục tiêu và các thành phần khác trong mô thực vật. Bài đánh giá này đi sâu vào các nguyên tắc cơ bản, đặc điểm nổi bật, những ưu điểm và ứng dụng của ba công nghệ MSI nổi bật. Hơn nữa, chúng tôi mong muốn hỗ trợ độc giả trong việc sử dụng MSI trong nghiên cứu sinh học thực vật của họ bằng cách thảo luận về các thách thức chính, đề xuất các giải pháp khả thi và làm sáng tỏ triển vọng tương lai liên quan đến công nghệ tiên tiến này.\u003C\u002Fjats:p>",{"EN":543,"VI":544},"Applications of mass spectrometry imaging in botanical research","Các Ứng Dụng của Phân Tích Quang Phổ Khối Trong Nghiên Cứu Thực Vật",{"VOID":546},"10.1007\u002Fs44307-024-00014-y",[548],"EN",[177],"https:\u002F\u002Flink.springer.com\u002F10.1007\u002Fs44307-024-00014-y",[552,572,588,604],{"id":553,"sortIndex":19,"researcher":18,"roles":554,"affiliations":555,"properties":565},"e016abcc-a1ec-4798-9138-c6af9385547e",[],[556],{"id":18,"sortIndex":19,"affiliation":557,"properties":18},{"id":558,"createTime":559,"updateTime":559,"relativeEntities":560,"slug":561,"properties":562,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"91e73e16-c445-4541-8043-be3e130b246d","2024-04-14T09:58:33.627+00:00",[],"State-Key-Laboratory-of-Traditional-Chinese-Medicine-School-of-Pharmaceutical-Sciences-Guangzhou-University-of-Chinese-Medicine-Guangzhou-510006-China",{"title":563},{"EN":564},"State Key Laboratory of Traditional Chinese Medicine\u002FSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China",{"openalex":566,"orcid":568,"title":570},{"VOID":567},"A5059868960",{"VOID":569},"https:\u002F\u002Forcid.org\u002F0000-0002-4920-152X",{"EN":571},"Yijun Chen",{"id":573,"sortIndex":96,"researcher":18,"roles":574,"affiliations":575,"properties":581},"2ba903dd-4d00-47e1-8062-18046ebe0184",[],[576],{"id":18,"sortIndex":19,"affiliation":577,"properties":18},{"id":558,"createTime":559,"updateTime":559,"relativeEntities":578,"slug":561,"properties":579,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":580},{"EN":564},{"openalex":582,"orcid":584,"title":586},{"VOID":583},"A5026465582",{"VOID":585},"https:\u002F\u002Forcid.org\u002F0000-0003-4957-0509",{"EN":587},"Hong‐Bin Wang",{"id":589,"sortIndex":109,"researcher":18,"roles":590,"affiliations":591,"properties":597},"8a01b7f3-6593-4032-a2fd-1fa29f40568d",[],[592],{"id":18,"sortIndex":19,"affiliation":593,"properties":18},{"id":558,"createTime":559,"updateTime":559,"relativeEntities":594,"slug":561,"properties":595,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":596},{"EN":564},{"openalex":598,"orcid":600,"title":602},{"VOID":599},"A5021672168",{"VOID":601},"https:\u002F\u002Forcid.org\u002F0000-0001-9148-9937",{"EN":603},"Haoli Jin",{"id":605,"sortIndex":26,"researcher":18,"roles":606,"affiliations":607,"properties":613},"a87e10f2-e0cc-46dd-9a33-7a8c9175b48f",[],[608],{"id":18,"sortIndex":19,"affiliation":609,"properties":18},{"id":558,"createTime":559,"updateTime":559,"relativeEntities":610,"slug":561,"properties":611,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":612},{"EN":564},{"openalex":614,"title":616},{"VOID":615},"A5077656240",{"EN":617},"Hai-Sheng Zeng",{"url":18,"publisher":619,"properties":18},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":620,"slug":10,"properties":621,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":624,"manageAffiliations":625,"indexDatabases":626,"url":23,"thumbnailPath":18,"statistic":627,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":622,"title":623},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":628,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":629,"totalCitation":19,"totalCitationByYear":630,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":631,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},{"total":19,"publishYear":18,"statisticByYear":633},{},[635,639,643,647,651,655,659,663,667,671,675,679,683,687,691,695,699,703,707,711,715,719,723,727,731,734,738,742,746,750,754,758,762,766,770,774,778,782,786,790,794,798,802,806,810,814,818,822,826,830,834,838,842,846,850,854,858,862,866,870,874,878,882,886,890,894,898,902,906,909,913,917,921,925,929,933,937,941,945,949,953,957,961,965,969,973,977,981,985,989,993,997,1001,1005,1009,1013,1017,1021,1025,1029,1033,1037,1041,1045,1049,1053,1057,1061,1065,1069,1073,1077,1081,1085,1089],{"id":18,"text":636,"url":18,"identifiers":637},"Baker TC, Han J, Borchers CH. 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Analyst. 2015;140:7696–709.",{"doi":650},"10.1039\u002FC5AN01065A",{"id":18,"text":652,"url":18,"identifiers":653},"Boskamp T, Casadonte R, Hauberg-Lotte L, et al. Cross-Normalization of MALDI Mass Spectrometry Imaging Data Improves Site-to-Site Reproducibility. Anal Chem. 2021;93:10584–92.",{"doi":654},"10.1021\u002Facs.analchem.1c01792",{"id":18,"text":656,"url":18,"identifiers":657},"Cabral EC, Ifa DR. DESI imaging of small molecules in biological tissues. Methods Mol Biol. 2015;1203:63–77.",{"doi":658},"10.1007\u002F978-1-4939-1357-2_7",{"id":18,"text":660,"url":18,"identifiers":661},"Cailletaud J, Bleye C, Dumont E, et al. Towards a spray-coating method for the detection of low-dose compounds in pharmaceutical tablets using surface-enhanced Raman chemical imaging (SER-CI). Talanta. 2018;188:584–92.",{"doi":662},"10.1016\u002Fj.talanta.2018.06.037",{"id":18,"text":664,"url":18,"identifiers":665},"Caprioli RM, Farmer TB, Gile J. Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS. Anal Chem. 1997;69:4751–60.",{"doi":666},"10.1021\u002Fac970888i",{"id":18,"text":668,"url":18,"identifiers":669},"Chu B, Chen Z, Shi H, et al. Fluorescence, ultrasonic and photoacoustic imaging for analysis and diagnosis of diseases. Chem Commun (camb). 2023;59:2399–412.",{"doi":670},"10.1039\u002FD2CC06654H",{"id":18,"text":672,"url":18,"identifiers":673},"Conceição RS, Perez CJ, Branco A, et al. Identification of Sassafras albidum alkaloids by high-performance thin-layer chromatography tandem mass spectrometry and mapping by desorption electrospray ionization mass spectrometry imaging. J Mass Spectrom. 2021;56:e4674.",{"doi":674},"10.1002\u002Fjms.4674",{"id":18,"text":676,"url":18,"identifiers":677},"Conceicao RS, Reis IMA, Cerqueira APM, et al. Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MS(n). Phytochem Anal. 2020;31:711–21.",{"doi":678},"10.1002\u002Fpca.2935",{"id":18,"text":680,"url":18,"identifiers":681},"Cooks RG, Ouyang Z, Takats Z, et al. Detection Technologies. Ambient mass spectrometry. Science. 2006;311:1566–70.",{"doi":682},"10.1126\u002Fscience.1119426",{"id":18,"text":684,"url":18,"identifiers":685},"Cordes J, Enzlein T, Marsching C, et al. M2aia—Interactive, fast, and memory-efficient analysis of 2D and 3D multi-modal mass spectrometry imaging data. GigaScience. 2021;10:giab049.",{"doi":686},"10.1093\u002Fgigascience\u002Fgiab049",{"id":18,"text":688,"url":18,"identifiers":689},"Cornett DS, Reyzer ML, Chaurand P, et al. MALDI imaging mass spectrometry: molecular snapshots of biochemical systems. Nat Methods. 2007;4:828–33.",{"doi":690},"10.1038\u002Fnmeth1094",{"id":18,"text":692,"url":18,"identifiers":693},"de Abreu LB, Augusti R, Schmidt L, et al. Desorption electrospray ionization mass spectrometry (DESI-MS) applied to the speciation of arsenic compounds from fern leaves. Anal Bioanal Chem. 2013;405:7643–51.",{"doi":694},"10.1007\u002Fs00216-013-6986-y",{"id":18,"text":696,"url":18,"identifiers":697},"Dong Y, Aharoni A. Image to insight: exploring natural products through mass spectrometry imaging. Nat Prod Rep. 2022;39:1510–30.",{"doi":698},"10.1039\u002FD2NP00011C",{"id":18,"text":700,"url":18,"identifiers":701},"Dreisbach D, Petschenka G, Spengler B, et al. 3D-surface MALDI mass spectrometry imaging for visualising plant defensive cardiac glycosides in Asclepias curassavica. Anal Bioanal Chem. 2021;413:2125–34.",{"doi":702},"10.1007\u002Fs00216-021-03177-y",{"id":18,"text":704,"url":18,"identifiers":705},"Duan S, Xu X. Accurate Simulations of Scanning Tunneling Microscope: Both Tip and Substrate States Matter. J Phys Chem Lett. 2023;14:6726–35.",{"doi":706},"10.1021\u002Facs.jpclett.3c01603",{"id":18,"text":708,"url":18,"identifiers":709},"Dueñas ME, Klein AT, Alexander LE, et al. High spatial resolution mass spectrometry imaging reveals the genetically programmed, developmental modification of the distribution of thylakoid membrane lipids among individual cells of maize leaf. Plant J. 2017;89:825–38.",{"doi":710},"10.1111\u002Ftpj.13422",{"id":18,"text":712,"url":18,"identifiers":713},"Dueñas ME, Larson EA, Lee YJ. Toward mass spectrometry imaging in the metabolomics scale: increasing metabolic coverage through multiple on-tissue chemical modifications. Front Plant Sci. 2019;10:860.",{"doi":714},"10.3389\u002Ffpls.2019.00860",{"id":18,"text":716,"url":18,"identifiers":717},"Dutkiewicz EP, Su CH, Lee HJ, et al. Visualizing vinca alkaloids in the petal of Catharanthus roseus using functionalized titanium oxide nanowire substrate for surface-assisted laser desorption\u002Fionization imaging mass spectrometry. Plant J. 2021;105:1123–33.",{"doi":718},"10.1111\u002Ftpj.15092",{"id":18,"text":720,"url":18,"identifiers":721},"Ellis SR, Paine MRL, Eijkel GB, et al. Automated, parallel mass spectrometry imaging and structural identification of lipids. Nat Methods. 2018;15:515–8.",{"doi":722},"10.1038\u002Fs41592-018-0010-6",{"id":18,"text":724,"url":18,"identifiers":725},"Esquenazi E, Dorrestein PC, Gerwick WH. Probing marine natural product defenses with DESI-imaging mass spectrometry. Proc Natl Acad Sci U S A. 2009;106:7269–70.",{"doi":726},"10.1073\u002Fpnas.0902840106",{"id":18,"text":728,"url":18,"identifiers":729},"Feldberg L, Dong Y, Heinig U, et al. DLEMMA-MS-imaging for identification of spatially localized metabolites and metabolic network map reconstruction. 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J Mass Spectrom. 2016;51:1168–79.",{"doi":1092},"10.1002\u002Fjms.3888",{"id":1094,"createTime":1095,"updateTime":1096,"relativeEntities":1097,"slug":1098,"properties":1099,"entityType":48,"verifyStatus":49,"verifyTime":1111,"verifyNote":51,"syncStatus":17,"languages":1112,"translateLanguages":1113,"viewCount":19,"primaryUrl":1114,"fullTextUrl":18,"authors":1115,"publicationType":132,"publisherRelationship":1207,"citationCount":19,"citationInfo":1221,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":1223,"isForceReanalyzing":154},"19639203-ff0f-4f37-8c07-353ae2898e12","2024-04-16T04:50:58.315+00:00","2025-02-19T18:29:01.204+00:00",[],"A-human-antibody-derived-from-original-SARS-CoV-2-infection-effectively-neutralizes-omicron",{"keywords":1100,"openalex":1101,"abstract":1103,"title":1106,"doi":1109},{"VI":165},{"VOID":1102},"W4391295840",{"EN":1104,"VI":1105},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) Variants of Concern (VOCs), such as the Omicron sub-variants, present significant challenges in pandemic control due to their capacity to escape antibodies and breach vaccine protections. Discovering antibodies that can tolerate mutations in VOCs and understanding their underlying mechanisms is crucial for developing therapeutics for COVID-19 patients, particularly those for whom other therapies may be unsuitable. Here, we report the neutralization of the Omicron variant by FD20, a broadly active human monoclonal antibody. In contrast to a clinically approved control antibody, FD20 neutralizes Omicron with comparable IC\u003Cjats:sub>50\u003C\u002Fjats:sub> values to those observed for previously circulating VOCs and the original strain reported in Wuhan. Leveraging structural information, we provide insights into its resilience against mutations in Omicron. The results encourage the prospective development of FD20 as a therapeutic option for COVID-19 caused by current and potentially future VOCs.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Biến thể đáng lo ngại (Variants of Concern - VOCs) của SARS-CoV-2 (Virus corona gây hội chứng hô hấp cấp tính nghiêm trọng 2), chẳng hạn như các biến thể phụ Omicron, đặt ra những thách thức lớn trong việc kiểm soát đại dịch do khả năng của chúng trong việc thoát khỏi kháng thể và vượt qua mức bảo vệ của vắc xin. Việc phát hiện ra các kháng thể có khả năng dung nạp sự đột biến trong các VOC và hiểu rõ các cơ chế bên dưới là rất quan trọng cho sự phát triển các liệu pháp điều trị cho bệnh nhân COVID-19, đặc biệt là đối với những bệnh nhân mà các liệu pháp khác có thể không phù hợp. Trong nghiên cứu này, chúng tôi báo cáo việc trung hòa biến thể Omicron bởi FD20, một kháng thể đơn dòng của con người hoạt động mạnh mẽ. Khác với một kháng thể đối chứng được phê duyệt lâm sàng, FD20 trung hòa Omicron với giá trị IC\u003Cjats:sub>50\u003C\u002Fjats:sub> tương đương với những gì được quan sát cho các VOC đã lưu hành trước đó và chủng gốc được báo cáo tại Vũ Hán. Tận dụng thông tin cấu trúc, chúng tôi cung cấp cái nhìn sâu sắc về khả năng chịu đựng đột biến của kháng thể này trong biến thể Omicron. Kết quả khuyến khích việc phát triển FD20 như một lựa chọn điều trị cho COVID-19 do các VOC hiện tại và có thể xảy ra trong tương lai gây ra.\u003C\u002Fjats:p>",{"EN":1107,"VI":1108},"A human antibody derived from original SARS-CoV-2 infection effectively neutralizes omicron","Kháng thể người có nguồn gốc từ nhiễm SARS-CoV-2 ban đầu trung hòa hiệu quả biến thể Omicron",{"VOID":1110},"10.1007\u002Fs44307-024-00011-1","2024-12-20T04:34:17.331+00:00",[548],[177],"https:\u002F\u002Flink.springer.com\u002F10.1007\u002Fs44307-024-00011-1",[1116,1136,1155,1171,1191],{"id":1117,"sortIndex":26,"researcher":18,"roles":1118,"affiliations":1119,"properties":1129},"dca19448-5631-41bd-a30f-a7218e11cdf5",[],[1120],{"id":18,"sortIndex":19,"affiliation":1121,"properties":18},{"id":1122,"createTime":1123,"updateTime":1123,"relativeEntities":1124,"slug":1125,"properties":1126,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"0f35d2a3-1340-4a86-8051-49fdc2d0d2fb","2024-04-16T04:50:58.335+00:00",[],"Shanghai-Institute-of-Immunity-and-Infection-Chinese-Academy-of-Sciences-320-Yueyang-Road-Shanghai-200030-China",{"title":1127},{"EN":1128},"Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200030, China",{"openalex":1130,"orcid":1132,"title":1134},{"VOID":1131},"A5028183150",{"VOID":1133},"https:\u002F\u002Forcid.org\u002F0000-0002-7753-4065",{"EN":1135},"Bingjie Zhou",{"id":1137,"sortIndex":96,"researcher":18,"roles":1138,"affiliations":1139,"properties":1148},"0b4fad45-1b0d-4f04-b309-049d29d69266",[],[1140],{"id":18,"sortIndex":19,"affiliation":1141,"properties":18},{"id":1142,"createTime":1143,"updateTime":1143,"relativeEntities":1144,"slug":18,"properties":1145,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"2f5815ae-5136-483d-9387-b141640ee13d","2024-01-08T04:53:42.324+00:00",[],{"title":1146},{"VI":1147},"Pasteurien College, Soochow University, Jiangsu, China",{"openalex":1149,"orcid":1151,"title":1153},{"VOID":1150},"A5032903400",{"VOID":1152},"https:\u002F\u002Forcid.org\u002F0000-0002-4706-1519",{"EN":1154},"Dimitri Lavillette",{"id":1156,"sortIndex":109,"researcher":18,"roles":1157,"affiliations":1158,"properties":1164},"553e244b-649d-40ca-b355-ef59bcc760b6",[],[1159],{"id":18,"sortIndex":19,"affiliation":1160,"properties":18},{"id":1142,"createTime":1143,"updateTime":1143,"relativeEntities":1161,"slug":18,"properties":1162,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":1163},{"VI":1147},{"openalex":1165,"orcid":1167,"title":1169},{"VOID":1166},"A5090609624",{"VOID":1168},"https:\u002F\u002Forcid.org\u002F0009-0007-5003-6801",{"EN":1170},"Huafeng Dong",{"id":1172,"sortIndex":19,"researcher":18,"roles":1173,"affiliations":1174,"properties":1184},"0855a36e-7cfe-431e-9ce0-1d81ce7bcbae",[],[1175],{"id":18,"sortIndex":19,"affiliation":1176,"properties":18},{"id":1177,"createTime":1178,"updateTime":1178,"relativeEntities":1179,"slug":1180,"properties":1181,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"9692c04a-8947-4f5e-a776-262fffeca674","2024-04-16T04:50:58.324+00:00",[],"Center-for-Excellence-in-Molecular-Cell-Science-Shanghai-Institute-of-Biochemistry-and-Cell-Biology-Chinese-Academy-of-Sciences-320-Yueyang-Road-Shanghai-200030-China",{"title":1182},{"EN":1183},"Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai, 200030, China",{"openalex":1185,"orcid":1187,"title":1189},{"VOID":1186},"A5061396145",{"VOID":1188},"https:\u002F\u002Forcid.org\u002F0000-0002-6113-1624",{"EN":1190},"Tingting Li",{"id":1192,"sortIndex":73,"researcher":18,"roles":1193,"affiliations":1194,"properties":1200},"ba92fa02-5f72-442d-b4d9-a103bce7cc1f",[],[1195],{"id":18,"sortIndex":19,"affiliation":1196,"properties":18},{"id":1177,"createTime":1178,"updateTime":1178,"relativeEntities":1197,"slug":1180,"properties":1198,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":1199},{"EN":1183},{"openalex":1201,"orcid":1203,"title":1205},{"VOID":1202},"A5026452363",{"VOID":1204},"https:\u002F\u002Forcid.org\u002F0000-0003-4729-4678",{"EN":1206},"Dianfan Li",{"url":18,"publisher":1208,"properties":18},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":1209,"slug":10,"properties":1210,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":1213,"manageAffiliations":1214,"indexDatabases":1215,"url":23,"thumbnailPath":18,"statistic":1216,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":1211,"title":1212},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":1217,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":1218,"totalCitation":19,"totalCitationByYear":1219,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":1220,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},{"total":19,"publishYear":18,"statisticByYear":1222},{},[1224,1228,1232,1236,1240,1244,1248,1252,1256,1260,1264,1268,1272,1276,1280,1284,1288,1292,1296,1300,1303,1307,1311,1315,1319,1323,1327,1331,1335,1339,1343,1347,1351,1355,1359,1363,1367,1371,1375,1379,1383,1387,1391,1395,1399],{"id":18,"text":1225,"url":18,"identifiers":1226},"Barnes CO, et al. 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Nature. 2020;581:215–20.",{"doi":1291},"10.1038\u002Fs41586-020-2180-5",{"id":18,"text":1293,"url":18,"identifiers":1294},"Li T, et al. Uncovering a conserved vulnerability site in SARS-CoV-2 by a human antibody. EMBO Mol Med. 2021a;13: e14544.",{"doi":1295},"10.15252\u002Femmm.202114544",{"id":18,"text":1297,"url":18,"identifiers":1298},"Li T, et al. A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection. Nat Commun. 2021b;12:4635.",{"doi":1299},"10.1038\u002Fs41467-021-24905-z",{"id":18,"text":1301,"url":18,"identifiers":1302},"Li T, et al. A synthetic nanobody targeting RBD protects hamsters from SARS-CoV-2 infection. Nat Commun. 2021c;12:4635.",{"doi":1299},{"id":18,"text":1304,"url":18,"identifiers":1305},"Li T, et al. Isolation, characterization, and structure-based engineering of a neutralizing nanobody against SARS-CoV-2. 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PLoS Pathog. 2021;17:e1009328.",{"doi":1398},"10.1371\u002Fjournal.ppat.1009328",{"id":18,"text":1400,"url":18,"identifiers":1401},"Zhou D, et al. Structural basis for the neutralization of SARS-CoV-2 by an antibody from a convalescent patient. Nat Struct Mol Biol. 2020;27:950–8.",{"doi":1402},"10.1038\u002Fs41594-020-0480-y",{"id":1404,"createTime":1405,"updateTime":1406,"relativeEntities":1407,"slug":1408,"properties":1409,"entityType":48,"verifyStatus":49,"verifyTime":1421,"verifyNote":51,"syncStatus":17,"languages":1422,"translateLanguages":1423,"viewCount":19,"primaryUrl":1424,"fullTextUrl":18,"authors":1425,"publicationType":132,"publisherRelationship":1503,"citationCount":19,"citationInfo":1517,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":1519,"isForceReanalyzing":154},"8c941d1e-3475-404b-8a00-310217600b9d","2024-04-11T16:13:58.481+00:00","2025-02-19T18:26:10.414+00:00",[],"Strategies-to-increase-the-robustness-of-microbial-cell-factories",{"keywords":1410,"openalex":1411,"abstract":1413,"title":1416,"doi":1419},{"VI":165},{"VOID":1412},"W4392365556",{"EN":1414,"VI":1415},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>Engineering microbial cell factories have achieved much progress in producing fuels, natural products and bulk chemicals. However, in industrial fermentation, microbial cells often face various predictable and stochastic disturbances resulting from intermediate metabolites or end product toxicity, metabolic burden and harsh environment. These perturbances can potentially decrease productivity and titer. Therefore, strain robustness is essential to ensure reliable and sustainable production efficiency. In this review, the current strategies to improve host robustness were summarized, including knowledge-based engineering approaches, such as transcription factors, membrane\u002Ftransporters and stress proteins, and the traditional adaptive laboratory evolution based on natural selection. Computation-assisted (e.g. GEMs, deep learning and machine learning) design of robust industrial hosts was also introduced. Furthermore, the challenges and future perspectives on engineering microbial host robustness are proposed to promote the development of green, efficient and sustainable biomanufacturers.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Các nhà máy tế bào vi sinh vật đã đạt được nhiều tiến bộ trong việc sản xuất nhiên liệu, sản phẩm tự nhiên và hóa chất số lượng lớn. Tuy nhiên, trong quá trình lên men công nghiệp, các tế bào vi sinh vật thường phải đối mặt với nhiều rối loạn có thể dự đoán và ngẫu nhiên do độc tính của các metabolite trung gian hoặc sản phẩm cuối cùng, gánh nặng chuyển hóa và môi trường khắc nghiệt. Những rối loạn này có thể giảm năng suất và mật độ sản phẩm. Do đó, độ bền của các chủng vi sinh vật là rất cần thiết để đảm bảo hiệu quả sản xuất đáng tin cậy và bền vững. Trong bài đánh giá này, các chiến lược hiện tại để cải thiện độ bền của chủ nhà đã được tóm tắt, bao gồm các phương pháp kỹ thuật dựa trên kiến thức, chẳng hạn như các yếu tố phiên mã, màng\u002F transporter và protein phản ứng stress, cùng với quá trình tiến hóa thích nghi truyền thống dựa trên chọn lọc tự nhiên. Thiết kế chủ vi sinh vật công nghiệp bền vững hỗ trợ bởi tính toán (ví dụ: GEMs, học sâu và học máy) cũng đã được giới thiệu. Hơn nữa, các thách thức và viễn cảnh trong tương lai về cải tiến độ bền của chủ vi sinh vật đã được đề xuất nhằm thúc đẩy phát triển các nhà sản xuất sinh học xanh, hiệu quả và bền vững.\u003C\u002Fjats:p>",{"EN":1417,"VI":1418},"Strategies to increase the robustness of microbial cell factories","Chiến lược tăng cường độ bền của nhà máy tế bào vi sinh 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China",{"openalex":1441,"orcid":1443,"title":1445},{"VOID":1442},"A5030104317",{"VOID":1444},"https:\u002F\u002Forcid.org\u002F0000-0001-5721-020X",{"EN":1446},"Ning Lin",{"id":1448,"sortIndex":96,"researcher":18,"roles":1449,"affiliations":1450,"properties":1461},"e64b7d68-3b6c-4332-b6f4-2f8c2990943d",[],[1451],{"id":18,"sortIndex":19,"affiliation":1452,"properties":18},{"id":1453,"createTime":1454,"updateTime":1455,"relativeEntities":1456,"slug":1457,"properties":1458,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"09d96e95-fea3-4ddd-a11d-8d94346c0a41","2024-04-11T16:13:59.533+00:00","2025-06-11T22:46:47.729+00:00",[],"Joint-Research-Center-of-Engineering-Biology-Technology-of-Sun-Yat-Sen-University-and-Tidetron-Bioworks-Guangzhou-510275-China",{"title":1459},{"EN":1460},"Joint Research Center of Engineering Biology Technology of Sun Yat-Sen University and Tidetron Bioworks, Guangzhou, 510275, China",{"openalex":1462,"orcid":1464,"title":1466},{"VOID":1463},"A5022912106",{"VOID":1465},"https:\u002F\u002Forcid.org\u002F0000-0002-1570-3214",{"EN":1467},"Jian-Zhong Liu",{"id":1469,"sortIndex":109,"researcher":18,"roles":1470,"affiliations":1471,"properties":1482},"5bc8f1a4-e405-42ab-971d-08574f3f654e",[],[1472],{"id":18,"sortIndex":19,"affiliation":1473,"properties":18},{"id":1474,"createTime":1475,"updateTime":1476,"relativeEntities":1477,"slug":1478,"properties":1479,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"08213ac6-d22e-4207-b58b-5365eef84665","2024-04-11T16:13:59.493+00:00","2025-06-11T21:08:20.285+00:00",[],"Tidetron-Bioworks-Technology-Guangzhou-Co-Ltd-Guangzhou-510399-China",{"title":1480},{"EN":1481},"Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou, 510399, China",{"openalex":1483,"title":1485},{"VOID":1484},"A5036950109",{"EN":1486},"Zhi‐Qian Zhang",{"id":1488,"sortIndex":19,"researcher":18,"roles":1489,"affiliations":1490,"properties":1496},"2df9868e-cf24-4273-ba7e-30b1dc9747d2",[],[1491],{"id":18,"sortIndex":19,"affiliation":1492,"properties":18},{"id":1432,"createTime":1433,"updateTime":1434,"relativeEntities":1493,"slug":1436,"properties":1494,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":1495},{"VI":1439},{"openalex":1497,"orcid":1499,"title":1501},{"VOID":1498},"A5070751588",{"VOID":1500},"https:\u002F\u002Forcid.org\u002F0000-0002-4053-9495",{"EN":1502},"Pei 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Chinese Sci Bull. 2023;2023(68):1626–36.",{"doi":1923},"10.1360\u002FTB-2022-1106",{"id":1925,"createTime":1926,"updateTime":1927,"relativeEntities":1928,"slug":1929,"properties":1930,"entityType":48,"verifyStatus":49,"verifyTime":1943,"verifyNote":51,"syncStatus":17,"languages":1944,"translateLanguages":1945,"viewCount":19,"primaryUrl":1946,"fullTextUrl":18,"authors":1947,"publicationType":132,"publisherRelationship":2075,"citationCount":19,"citationInfo":2093,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":2095,"isForceReanalyzing":154},"458f2535-87c2-45ac-99cb-e4199294b4e5","2024-04-16T16:52:42.553+00:00","2025-02-19T18:25:13.258+00:00",[],"Importance-of-pre-mRNA-splicing-and-its-study-tools-in-plants",{"keywords":1931,"openalex":1933,"abstract":1935,"title":1938,"doi":1941},{"VI":1932},"quá trình cắt nối tiền mRNA, đa dạng phiên mã, sinh vật nhân chuẩn, stress phi sinh học, nghiên cứu di truyền, kỹ thuật omics",{"VOID":1934},"W4391647954",{"EN":1936,"VI":1937},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>Alternative splicing (AS) significantly enriches the diversity of transcriptomes and proteomes, playing a pivotal role in the physiology and development of eukaryotic organisms. With the continuous advancement of high-throughput sequencing technologies, an increasing number of novel transcript isoforms, along with factors related to splicing and their associated functions, are being unveiled. In this review, we succinctly summarize and compare the different splicing mechanisms across prokaryotes and eukaryotes. Furthermore, we provide an extensive overview of the recent progress in various studies on AS covering different developmental stages in diverse plant species and in response to various abiotic stresses. Additionally, we discuss modern techniques for studying the functions and quantification of AS transcripts, as well as their protein products. By integrating genetic studies, quantitative methods, and high-throughput omics techniques, we can discover novel transcript isoforms and functional splicing factors, thereby enhancing our understanding of the roles of various splicing modes in different plant species.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Quá trình cắt nối thay thế (AS) làm giàu đáng kể sự đa dạng của hệ gen phiên mã và proteom, đóng vai trò chủ chốt trong sinh lý học và sự phát triển của các sinh vật nhân chuẩn. Với sự tiến bộ không ngừng của các công nghệ giải trình tự gen cao thông lượng, ngày càng nhiều các isoform phiên mã mới, cùng với các yếu tố liên quan đến quá trình cắt nối và các chức năng liên quan của chúng, đang được khám phá. Trong bài tổng quan này, chúng tôi tóm tắt ngắn gọn và so sánh các cơ chế cắt nối khác nhau giữa vi khuẩn và sinh vật nhân chuẩn. Hơn nữa, chúng tôi cung cấp cái nhìn tổng quan rộng rãi về những tiến bộ gần đây trong nhiều nghiên cứu về AS, bao gồm các giai đoạn phát triển khác nhau ở các loài thực vật đa dạng và phản ứng với nhiều loại stress phi sinh học khác nhau. Thêm vào đó, chúng tôi thảo luận về các kỹ thuật hiện đại để nghiên cứu chức năng và định lượng các phiên bản AS, cũng như các sản phẩm protein của chúng. Bằng cách tích hợp các nghiên cứu di truyền, các phương pháp định lượng và các kỹ thuật omics cao thông lượng, chúng ta có thể khám phá các isoform phiên mã mới và các yếu tố cắt nối chức năng, từ đó nâng cao sự hiểu biết của chúng ta về vai trò của các dạng cắt nối khác nhau ở các loài thực vật khác nhau.\u003C\u002Fjats:p>",{"EN":1939,"VI":1940},"Importance of pre-mRNA splicing and its study tools in plants","Tầm quan trọng của quá trình cắt nối mRNA tiền thân và các công cụ nghiên cứu của nó trong thực vật",{"VOID":1942},"10.1007\u002Fs44307-024-00009-9","2025-01-11T22:21:59.262+00:00",[548],[177],"https:\u002F\u002Flink.springer.com\u002F10.1007\u002Fs44307-024-00009-9",[1948,1968,1990,2011,2028,2043,2060],{"id":1949,"sortIndex":96,"researcher":18,"roles":1950,"affiliations":1951,"properties":1961},"1c2c8b77-e008-499f-86bc-883305eced5b",[],[1952],{"id":1953,"sortIndex":19,"affiliation":1954,"properties":18},"a568ec24-f5bd-4732-ae4d-b480831a734c",{"id":1955,"createTime":1956,"updateTime":1956,"relativeEntities":1957,"slug":18,"properties":1958,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"91fde863-8acd-46f8-82be-0b250cb4e056","2023-12-15T02:35:24.339+00:00",[],{"title":1959},{"VI":1960},"National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for Research and Development of Fine Chemicals, Guizhou University, Guiyang, China",{"openalex":1962,"orcid":1964,"title":1966},{"VOID":1963},"A5049507373",{"VOID":1965},"https:\u002F\u002Forcid.org\u002F0000-0002-2764-6827",{"EN":1967},"Jingxin Li",{"id":1969,"sortIndex":245,"researcher":18,"roles":1970,"affiliations":1971,"properties":1983},"3bff47f9-63ac-4f5c-9631-f0430a91508b",[],[1972],{"id":1973,"sortIndex":19,"affiliation":1974,"properties":18},"3a2dc50a-2268-4101-b217-ec81bd89d032",{"id":1975,"createTime":1976,"updateTime":1977,"relativeEntities":1978,"slug":1979,"properties":1980,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"85565924-691d-4d12-b7ad-e2113198af7d","2024-01-12T23:08:13.198+00:00","2024-11-26T12:08:18.667+00:00",[],"Department-of-Molecular-Biosciences-and-Bioengineering-University-of-Hawaii-at-Manoa-Honolulu-HI-96822-USA",{"title":1981},{"VI":1982},"Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA",{"openalex":1984,"orcid":1986,"title":1988},{"VOID":1985},"A5013210570",{"VOID":1987},"https:\u002F\u002Forcid.org\u002F0000-0002-7903-4777",{"EN":1989},"Zhimin Du",{"id":1991,"sortIndex":73,"researcher":18,"roles":1992,"affiliations":1993,"properties":2004},"381c4e5b-9894-467d-bc77-4c35ad9ec570",[],[1994],{"id":1995,"sortIndex":19,"affiliation":1996,"properties":18},"150f2728-a0b3-43d7-8ab8-53377aeee8eb",{"id":1997,"createTime":1998,"updateTime":1998,"relativeEntities":1999,"slug":2000,"properties":2001,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"a1babb12-6525-466b-89d9-c8868532c86e","2024-04-16T16:52:42.618+00:00",[],"National-Key-Laboratory-of-Wheat-Improvement-College-of-Life-Science-Shandong-Agricultural-University-Taian-Shandong-China",{"title":2002},{"EN":2003},"National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Taian, Shandong, China",{"openalex":2005,"orcid":2007,"title":2009},{"VOID":2006},"A5083484231",{"VOID":2008},"https:\u002F\u002Forcid.org\u002F0009-0006-0101-4174",{"EN":2010},"Yinggao Liu",{"id":2012,"sortIndex":19,"researcher":18,"roles":2013,"affiliations":2014,"properties":2021},"a0cdbbb8-53d7-47b0-9716-d6ae0ff14e0e",[],[2015],{"id":2016,"sortIndex":19,"affiliation":2017,"properties":18},"b0313292-0c2f-47c2-b8dd-0a06ca845454",{"id":1997,"createTime":1998,"updateTime":1998,"relativeEntities":2018,"slug":2000,"properties":2019,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":2020},{"EN":2003},{"openalex":2022,"orcid":2024,"title":2026},{"VOID":2023},"A5054795265",{"VOID":2025},"https:\u002F\u002Forcid.org\u002F0000-0002-3192-6020",{"EN":2027},"Yue 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Alternative Splicing, An Overlooked Defense Frontier of Plants with Respect to Bacterial Infection. J Agric Food Chem. 2023.",{"doi":2795},"10.1021\u002Facs.jafc.3c04163",{"id":18,"text":2797,"url":18,"identifiers":2798},"Xin R, Kathare PK, Huq E. Coordinated regulation of pre-mRNA splicing by the SFPS-RRC1 complex to promote photomorphogenesis. Plant Cell. 2019;31(9):2052–69.",{"doi":2799},"10.1105\u002Ftpc.18.00786",{"id":18,"text":2801,"url":18,"identifiers":2802},"Xiong F, Liu HH, Duan CY, Zhang BK, Wei G, Zhang Y, Li S. Arabidopsis JANUS regulates embryonic pattern formation through Pol II-mediated transcription of WOX2 and PIN7. Iscience. 2019a;19:1179–88.",{"doi":2803},"10.1016\u002Fj.isci.2019.09.004",{"id":18,"text":2805,"url":18,"identifiers":2806},"Xiong F, Ren JJ, Yu Q, Wang YY, Kong LJ, Otegui MS, Wang XL. At BUD 13 affects pre-mRNA splicing and is essential for embryo development in Arabidopsis. Plant J. 2019b;98(4):714–26.",{"doi":2807},"10.1111\u002Ftpj.14268",{"id":18,"text":2809,"url":18,"identifiers":2810},"Xiong F, Ren JJ, Yu Q, Wang YY, Lu CC, Kong LJ, Otegui MS, Wang XL. Atu2 AF 65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI 5 and FLC in Arabidopsis. New Phytol. 2019c;223(1):277–92.",{"doi":2811},"10.1111\u002Fnph.15756",{"id":18,"text":2813,"url":18,"identifiers":2814},"Xiong F, Ren JJ, Wang YY, Zhou Z, Qi HD, Otegui MS, Wang XL. An Arabidopsis retention and splicing complex regulates root and embryo development through pre-mRNA splicing. Plant Physiol. 2022;190(1):621–39.",{"doi":2815},"10.1093\u002Fplphys\u002Fkiac256",{"id":18,"text":2817,"url":18,"identifiers":2818},"Xiu Z, Sun F, Shen Y, Zhang X, Jiang R, Bonnard G, Zhang J, Tan BC. EMPTY PERICARP 16 is required for mitochondrial nad2 intron 4 cis-splicing, complex I assembly and seed development in maize. Plant J. 2016;85(4):507–19.",{"doi":2819},"10.1111\u002Ftpj.13122",{"id":18,"text":2821,"url":18,"identifiers":2822},"Xu T, Kim BM, Kwak KJ, Jung HJ, Kang H. The Arabidopsis homolog of human minor spliceosomal protein U11–48K plays a crucial role in U12 intron splicing and plant development. J Exp Bot. 2016;67(11):3397–406.",{"doi":2823},"10.1093\u002Fjxb\u002Ferw158",{"id":18,"text":2825,"url":18,"identifiers":2826},"Yan Z, Shi H, Liu Y, Jing M, Han Y. KHZ1 and KHZ2, novel members of the autonomous pathway, repress the splicing efficiency of FLC pre-mRNA in Arabidopsis. J Exp Bot. 2020;71(4):1375–86.",{"doi":2827},"10.1093\u002Fjxb\u002Ferz499",{"id":18,"text":2829,"url":18,"identifiers":2830},"Yan X, Bai D, Song H, Lin K, Pang E. Alternative splicing during fruit development among fleshy fruits. BMC Genomics. 2021;22(1):1–14.",{"doi":2831},"10.1186\u002Fs12864-021-08111-1",{"id":18,"text":2833,"url":18,"identifiers":2834},"Yan T, Heng Y, Wang W, Li J, Deng XW. SWELLMAP 2, a phyB-interacting splicing factor, negatively regulates seedling photomorphogenesis in Arabidopsis. Front Plant Sci. 2022;13:836519.",{"doi":2835},"10.3389\u002Ffpls.2022.836519",{"id":18,"text":2837,"url":18,"identifiers":2838},"Yang YZ, Ding S, Wang Y, Wang HC, Liu XY, Sun F, Xu C, Liu B, Tan BC. PPR20 is required for the cis-splicing of mitochondrial nad2 intron 3 and seed development in maize. Plant Cell Physiol. 2020;61(2):370–80.",{"doi":2839},"10.1093\u002Fpcp\u002Fpcz204",{"id":18,"text":2841,"url":18,"identifiers":2842},"Yang YZ, Ding S, Liu XY, Tang JJ, Wang Y, Sun F, Xu C, Tan BC. EMP32 is required for the cis-splicing of nad7 intron 2 and seed development in maize. RNA Biol. 2021;18(4):499–509.",{"doi":2843},"10.1080\u002F15476286.2020.1817267",{"id":18,"text":2845,"url":18,"identifiers":2846},"Yu H, Tian C, Yu Y, Jiao Y. Transcriptome survey of the contribution of alternative splicing to proteome diversity in Arabidopsis thaliana. Mol Plant. 2016;9(5):749–52.",{"doi":2847},"10.1016\u002Fj.molp.2015.12.018",{"id":18,"text":2849,"url":18,"identifiers":2850},"Yu K, Feng M, Yang G, Sun L, Qin Z, Cao J, Wen J, Li H, Zhou Y, Chen X. Changes in alternative splicing in response to domestication and polyploidization in wheat. Plant Physiol. 2020;184(4):1955–68.",{"doi":2851},"10.1104\u002Fpp.20.00773",{"id":18,"text":2853,"url":18,"identifiers":2854},"Yu H, Du Q, Campbell M, Yu B, Walia H, Zhang C. Genome-wide discovery of natural variation in pre-mRNA splicing and prioritising causal alternative splicing to salt stress response in rice. New Phytol. 2021;230(3):1273–87.",{"doi":2855},"10.1111\u002Fnph.17189",{"id":18,"text":2857,"url":18,"identifiers":2858},"Yuan N, Wang J, Zhou Y, An D, Xiao Q, Wang W, Wu Y. EMB-7L is required for embryogenesis and plant development in maize involved in RNA splicing of multiple chloroplast genes. Plant Sci. 2019;287:110203.",{"doi":2859},"10.1016\u002Fj.plantsci.2019.110203",{"id":18,"text":2861,"url":18,"identifiers":2862},"Zhang H, Lin C, Gu L. Light regulation of alternative Pre-mRNA splicing in plants. Photochem Photobiol. 2017a;93(1):159–65.",{"doi":2863},"10.1111\u002Fphp.12680",{"id":18,"text":2865,"url":18,"identifiers":2866},"Zhang Y, Ma X, Xie X, Liu YG. CRISPR\u002FCas9-based genome editing in plants. Prog Mol Biol Transl Sci. 2017b;149:133–50.",{"doi":2867},"10.1016\u002Fbs.pmbts.2017.03.008",{"id":18,"text":2869,"url":18,"identifiers":2870},"Zhang D, Chen MX, Zhu FY, Zhang J, Liu YG. Emerging functions of plant Serine\u002FArginine-Rich (SR) proteins: Lessons from animals. Crit Rev Plant Sci. 2020;39(2):173–94.",{"doi":2871},"10.1080\u002F07352689.2020.1770942",{"id":18,"text":2873,"url":18,"identifiers":2874},"Zhang Q, Zhang W, Wei J, Gao Z, Guan J, Cui Z, Wang X. SKIP regulates ABA signaling through alternative splicing in Arabidopsis. Plant Cell Physiol. 2022;63(4):494–507.",{"doi":2875},"10.1093\u002Fpcp\u002Fpcac014",{"id":18,"text":2877,"url":18,"identifiers":2878},"Zhang H, Kumimoto RW, Anver S, Harmer SL. XAP5 CIRCADIAN TIMEKEEPER regulates RNA splicing and the circadian clock by genetically separable pathways. Plant Physiol. 2023a;192:kiad193.",{"doi":2879},"10.1093\u002Fplphys\u002Fkiad193",{"id":18,"text":2881,"url":18,"identifiers":2882},"Zhang Q, Chen C, Wang Y, He M, Li Z, Shen L, Li Q, Zhu L, Ren D, Hu J. OsPPR11 encoding P-type PPR protein that affects group II intron splicing and chloroplast development. Plant Cell Rep. 2023b;42(2):421–31.",{"doi":2883},"10.1007\u002Fs00299-020-02644-7",{"id":18,"text":2885,"url":18,"identifiers":2886},"Zheng P, Liu Y, Liu X, Huang Y, Sun F, Wang W, Chen H, Jan M, Zhang C, Yuan Y. OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice. Theor Appl Genet. 2021;134:923–40.",{"doi":2887},"10.1007\u002Fs00122-020-03742-6",{"id":18,"text":2889,"url":18,"identifiers":2890},"Zheng J, Wen S, Yu Z, Luo K, Rong J, Ding M. Alternative splicing during fiber development in G. hirsutum. Int J Mol Sci. 2023;24(14):11812.",{"doi":2891},"10.3390\u002Fijms241411812",{"id":18,"text":2893,"url":18,"identifiers":2894},"Zhu J, Wang X, Xu Q, Zhao S, Tai Y, Wei C. Global dissection of alternative splicing uncovers transcriptional diversity in tissues and associates with the flavonoid pathway in tea plant (Camellia sinensis). BMC Plant Biol. 2018;18(1):1–12.",{"doi":2895},"10.1186\u002Fs12870-018-1497-9",{"id":18,"text":2897,"url":18,"identifiers":2898},"Zhu Y, Wu W, Shao W, Chen J, Shi X, Ma X, Xu YZ, Huang W, Huang J. SPLICING FACTOR1 is important in chloroplast development under cold stress. Plant Physiol. 2020;184(2):973–87.",{"doi":2899},"10.1104\u002Fpp.20.00706",{"id":18,"text":2901,"url":18,"identifiers":2902},"Zhu FY, Chen X, Song YC, Lam LPY, Tobimatsu Y, Gao B, Chen MX, Cao FL. SWATH-MS-based proteogenomic analysis reveals the involvement of alternative splicing in poplar upon lead stress. Genome Res. 2023;33(3):371–85.",{"doi":2903},"10.1101\u002Fgr.277473.122",{"id":18,"text":2905,"url":18,"identifiers":2906},"Zou C, Liu D, Wu P, Wang Y, Gai Z, Liu L, Yang F, Li C, Guo G. Transcriptome analysis of sugar beet (Beta vulgaris L.) in response to alkaline stress. Plant Mol Biol. 2020;102:645–57.",{"doi":2907},"10.1007\u002Fs11103-020-00971-7",{"id":2909,"createTime":2910,"updateTime":2911,"relativeEntities":2912,"slug":2913,"properties":2914,"entityType":48,"verifyStatus":49,"verifyTime":2926,"verifyNote":51,"syncStatus":17,"languages":2927,"translateLanguages":2928,"viewCount":19,"primaryUrl":2929,"fullTextUrl":18,"authors":2930,"publicationType":132,"publisherRelationship":3003,"citationCount":19,"citationInfo":3017,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":3019,"isForceReanalyzing":154},"088c6e53-0237-484e-a159-3066e8cbf17e","2024-04-15T20:51:18.595+00:00","2025-02-19T18:24:15.853+00:00",[],"Unraveling-the-role-of-autophagy-regulation-in-Crohn-s-disease-from-genetic-mechanisms-to-potential-therapeutics",{"keywords":2915,"openalex":2916,"abstract":2918,"title":2921,"doi":2924},{"VI":165},{"VOID":2917},"W4393054775",{"EN":2919,"VI":2920},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>Autophagy serves as the primary intracellular degradation mechanism in which damaged organelles and self-cytoplasmic proteins are transported to the lysosome for degradation. Crohn's disease, an idiopathic chronic inflammatory disorder of the gastrointestinal tract, manifests in diverse regions of the digestive system. Recent research suggests that autophagy modulation may be a new avenue for treating Crohn's disease, and several promising small-molecule modulators of autophagy have been reported as therapeutic options. In this review, we discuss in detail how mutations in autophagy-related genes function in Crohn's disease and summarize the modulatory effects on autophagy of small-molecule drugs currently used for Crohn's disease treatment. Furthermore, we delve into the therapeutic potential of small-molecule autophagy inducers on Crohn's disease, emphasizing the prospects for development in this field. We aim to highlight the significance of autophagy modulation in Crohn's disease, with the aspiration of contributing to the development of more efficacious treatments that can alleviate their suffering, and improve their quality of life.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Tự dị hóa hoạt động như cơ chế phân hủy nội bào chính, giúp vận chuyển các bào quan bị hư hại và protein tự bào chất đến không bào lysosome để phân hủy. Bệnh Crohn, một rối loạn viêm mãn tính không rõ nguyên nhân của đường tiêu hóa, thể hiện ở nhiều vùng khác nhau của hệ thống tiêu hóa. Nghiên cứu gần đây cho thấy việc điều chỉnh tự dị hóa có thể là một hướng đi mới trong điều trị bệnh Crohn, và một số chất điều chỉnh tự dị hóa phân tử nhỏ đầy hứa hẹn đã được báo cáo như các lựa chọn điều trị. Trong bài đánh giá này, chúng tôi sẽ thảo luận chi tiết về cách các đột biến trong các gen liên quan đến tự dị hóa hoạt động trong bệnh Crohn và tóm tắt các tác động điều chỉnh lên tự dị hóa của các loại thuốc phân tử nhỏ hiện đang được sử dụng để điều trị bệnh Crohn. Hơn nữa, chúng tôi cũng sẽ đi sâu vào tiềm năng điều trị của các tác nhân kích thích tự dị hóa phân tử nhỏ đối với bệnh Crohn, nhấn mạnh triển vọng phát triển trong lĩnh vực này. Chúng tôi nhằm mục đích làm nổi bật tầm quan trọng của việc điều chỉnh tự dị hóa trong bệnh Crohn, với hy vọng đóng góp vào sự phát triển của các phương pháp điều trị hiệu quả hơn có thể giảm bớt đau khổ của bệnh nhân và cải thiện chất lượng cuộc sống của họ.\u003C\u002Fjats:p>",{"EN":2922,"VI":2923},"Unraveling the role of autophagy regulation in Crohn's disease: from genetic mechanisms to potential therapeutics","Khám Phá Vai Trò Của Việc Điều Chỉnh Tự Dị hóa Trong Bệnh Crohn: Từ Cơ Chế Di Truyền Đến Các Phương Pháp Điều Trị Tiềm Năng",{"VOID":2925},"10.1007\u002Fs44307-024-00021-z","2025-01-24T15:04:49.447+00:00",[548],[177],"https:\u002F\u002Flink.springer.com\u002F10.1007\u002Fs44307-024-00021-z",[2931,2952,2968,2987],{"id":2932,"sortIndex":19,"researcher":18,"roles":2933,"affiliations":2934,"properties":2945},"b6e503f8-f954-4615-9183-b98db42e6193",[],[2935],{"id":18,"sortIndex":19,"affiliation":2936,"properties":18},{"id":2937,"createTime":2938,"updateTime":2939,"relativeEntities":2940,"slug":2941,"properties":2942,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"69aada00-1b3a-49ec-b9d7-ea8fb4907d50","2023-12-25T22:29:26.233+00:00","2024-09-01T02:24:02.623+00:00",[],"Sichuan-Engineering-Research-Center-for-Biomimetic-Synthesis-of-Natural-Drugs-School-of-Life-Science-and-Engineering-Southwest-Jiaotong-University-Chengdu-610031-China",{"title":2943},{"VI":2944},"Sichuan Engineering Research Center for Biomimetic Synthesis of Natural Drugs, School of Life Science and Engineering, Southwest Jiaotong University, Chengdu, 610031, China",{"openalex":2946,"orcid":2948,"title":2950},{"VOID":2947},"A5024372183",{"VOID":2949},"https:\u002F\u002Forcid.org\u002F0000-0003-4652-9156",{"EN":2951},"Yang Zhi-qiang",{"id":2953,"sortIndex":96,"researcher":18,"roles":2954,"affiliations":2955,"properties":2961},"1fc62379-809d-49cd-a02d-383670b34c8c",[],[2956],{"id":18,"sortIndex":19,"affiliation":2957,"properties":18},{"id":2937,"createTime":2938,"updateTime":2939,"relativeEntities":2958,"slug":2941,"properties":2959,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},[],{"title":2960},{"VI":2944},{"openalex":2962,"orcid":2964,"title":2966},{"VOID":2963},"A5003547177",{"VOID":2965},"https:\u002F\u002Forcid.org\u002F0000-0002-6905-2189",{"EN":2967},"Lan Zhang",{"id":2969,"sortIndex":109,"researcher":18,"roles":2970,"affiliations":2971,"properties":2980},"13086585-5637-47e1-aabf-cc06ab52f4c7",[],[2972],{"id":18,"sortIndex":19,"affiliation":2973,"properties":18},{"id":2974,"createTime":2975,"updateTime":2975,"relativeEntities":2976,"slug":18,"properties":2977,"entityType":67,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19},"4b420720-ba31-4989-984e-3dfdfdfd41ec","2023-12-07T17:43:25.787+00:00",[],{"title":2978},{"VI":2979},"Department of Biotherapy, Cancer Center and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, 610041, China",{"openalex":2981,"orcid":2983,"title":2985},{"VOID":2982},"A5090815103",{"VOID":2984},"https:\u002F\u002Forcid.org\u002F0000-0002-3603-6617",{"EN":2986},"Bo 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DW, Yang Y, Hutti JE, Madhavarapu S, Kelliher MA, Cantley LC. 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Cell Res. 2017;27:184–201.",{"doi":3704},"10.1038\u002Fcr.2016.146",{"id":3706,"createTime":3707,"updateTime":3708,"relativeEntities":3709,"slug":3710,"properties":3711,"entityType":48,"verifyStatus":17,"verifyTime":3723,"verifyNote":3724,"syncStatus":17,"languages":3725,"translateLanguages":3726,"viewCount":19,"primaryUrl":3727,"fullTextUrl":18,"authors":3728,"publicationType":132,"publisherRelationship":3804,"citationCount":19,"citationInfo":3821,"publishDate":18,"publishYear":18,"citationAnalyzeStatus":17,"lastCitationAnalyze":18,"indexDatabases":18,"openAccess":18,"references":3823,"isForceReanalyzing":154},"3fdf098e-9985-486a-a010-22ed1dd3c5f7","2024-04-21T02:53:48.470+00:00","2025-02-19T18:23:17.688+00:00",[],"Genetic-evidence-for-functions-of-Chloroplast-CA-in-Pyropia-yezoensis-decreased-CCM-but-increased-starch-accumulation",{"keywords":3712,"openalex":3713,"abstract":3715,"title":3718,"doi":3721},{"VI":165},{"VOID":3714},"W4394849331",{"EN":3716,"VI":3717},"\u003Cjats:title>Abstract\u003C\u002Fjats:title>\u003Cjats:p>In response to the changing intertidal environment, intertidal macroalgae have evolved complicated Ci utilization mechanisms. However, our knowledge regarding the CO\u003Cjats:sub>2\u003C\u002Fjats:sub> concentrating mechanism (CCM) of macroalgae is limited. Carbonic anhydrase (CA), a key component of CCM, plays essential roles in many physiological reactions in various organisms. While many genes encode CA in the \u003Cjats:italic>Pyropia yezoensis\u003C\u002Fjats:italic> genome, the exact function of specific CA in \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> remains elusive. To explore the particular function of chloroplast CA in intertidal macroalgae, we produced chloroplast-localized βCA1 knockdown mutants of \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> through RNA interference, and \u003Cjats:italic>Pyβca1i\u003C\u002Fjats:italic> mutants (hereinafter referred to as \u003Cjats:italic>ca1i\u003C\u002Fjats:italic>) showed a notable decrease in leaf area and overall biomass, as well as decreased soluble protein and unsaturated fatty acid content under different DIC conditions. However, \u003Cjats:italic>ca1i\u003C\u002Fjats:italic> mutants showed relatively higher starch content compared to the wild-type. The activity of enzymes involved in the Calvin cycle, photorespiration, Pentose-phosphate pathway, and floridean starch synthesis of \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> indicated an effective starch accumulation pathway after the interference of βCA1. All results suggest that the decreased activity of PyβCA1 impaired the CCM and development of thalli of \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic>, but stimulated starch accumulation in the cytoplasm through feedback to the photorespiration pathway and pentose phosphate pathway to replenish intermediates for the Calvin cycle. This study is the first to explore the specific function of chloroplast CA in intertidal macroalgae using genomic technology. The results provide valuable insights into the adaption mechanisms of intertidal macroalgae to their environment.\u003C\u002Fjats:p>","\u003Cjats:title>Tóm tắt\u003C\u002Fjats:title>\u003Cjats:p>Để phản ứng với môi trường lồi lõm thay đổi, macroalgae lồi lõm đã tiến hóa ra các cơ chế sử dụng CO\u003Cjats:sub>2\u003C\u002Fjats:sub> phức tạp. Tuy nhiên, kiến thức của chúng ta về cơ chế tập trung CO\u003Cjats:sub>2\u003C\u002Fjats:sub> (CCM) của macroalgae còn hạn chế. Carbonic anhydrase (CA), một thành phần chính của CCM, đóng vai trò thiết yếu trong nhiều phản ứng sinh lý ở các loài khác nhau. Trong khi nhiều gen mã hóa CA trong bộ gen \u003Cjats:italic>Pyropia yezoensis\u003C\u002Fjats:italic>, chức năng chính xác của CA cụ thể trong \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> vẫn chưa rõ ràng. Để khám phá chức năng đặc biệt của CA lạp thể trong macroalgae lồi lõm, chúng tôi đã tạo ra các đột biến giảm biểu hiện βCA1 lạp thể của \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> thông qua giao thoa RNA, và các đột biến \u003Cjats:italic>Pyβca1i\u003C\u002Fjats:italic> (sau đây gọi là \u003Cjats:italic>ca1i\u003C\u002Fjats:italic>) cho thấy diện tích lá và sinh khối tổng thể giảm đáng kể, cũng như giảm protein hòa tan và hàm lượng axit béo không bão hòa dưới các điều kiện DIC khác nhau. Tuy nhiên, đột biến \u003Cjats:italic>ca1i\u003C\u002Fjats:italic> cho thấy hàm lượng tinh bột tương đối cao hơn so với kiểu hình hoang dã. Hoạt động của các enzyme tham gia vào chu trình Calvin, quá trình quang hô hấp, con đường pentose-phosphate và tổng hợp tinh bột floridean của \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic> cho thấy một con đường tích lũy tinh bột hiệu quả sau khi can thiệp βCA1. Tất cả các kết quả cho thấy rằng hoạt động giảm của PyβCA1 làm suy yếu CCM và sự phát triển của thalli của \u003Cjats:italic>P. yezoensis\u003C\u002Fjats:italic>, nhưng kích thích tích lũy tinh bột trong tế bào chất thông qua phản hồi tới con đường quang hô hấp và con đường pentose phosphate để bổ sung các trung gian cho chu trình Calvin. Nghiên cứu này lần đầu tiên khám phá chức năng cụ thể của CA lạp thể trong macroalgae lồi lõm bằng công nghệ di truyền. Các kết quả cung cấp cái nhìn quý giá về các cơ chế thích ứng của macroalgae lồi lõm đối với môi trường của chúng.\u003C\u002Fjats:p>",{"EN":3719,"VI":3720},"Genetic evidence for functions of Chloroplast CA in Pyropia yezoensis: decreased CCM but increased starch accumulation","Bằng chứng di truyền cho chức năng của CA lạp thể trong Pyropia yezoensis: giảm CCM nhưng gia tăng tích lũy tinh bột",{"VOID":3722},"10.1007\u002Fs44307-024-00019-7","2025-01-22T11:31:57.247+00:00","Author affiliation is blank",[548],[177],"https:\u002F\u002Flink.springer.com\u002F10.1007\u002Fs44307-024-00019-7",[3729,3740,3749,3760,3771,3782,3793],{"id":3730,"sortIndex":296,"researcher":18,"roles":3731,"affiliations":3732,"properties":3733},"ce497e5c-fc0e-472a-b273-677b71225c34",[],[],{"openalex":3734,"orcid":3736,"title":3738},{"VOID":3735},"A5078979375",{"VOID":3737},"https:\u002F\u002Forcid.org\u002F0000-0001-9307-3994",{"EN":3739},"Guangce Wang",{"id":3741,"sortIndex":73,"researcher":18,"roles":3742,"affiliations":3743,"properties":3744},"d9123f9c-947d-4164-9fa2-fc575c2455f1",[],[],{"openalex":3745,"title":3747},{"VOID":3746},"A5024842511",{"EN":3748},"Zhizhuo Shao",{"id":3750,"sortIndex":245,"researcher":18,"roles":3751,"affiliations":3752,"properties":3753},"f2ad0a74-e16c-4f5d-b3e9-2689ea557512",[],[],{"openalex":3754,"orcid":3756,"title":3758},{"VOID":3755},"A5058065156",{"VOID":3757},"https:\u002F\u002Forcid.org\u002F0000-0001-7646-2429",{"EN":3759},"Zhi‐Yan Du",{"id":3761,"sortIndex":109,"researcher":18,"roles":3762,"affiliations":3763,"properties":3764},"b1ff453b-16f6-4fe8-9a14-dcd469a80b8e",[],[],{"openalex":3765,"orcid":3767,"title":3769},{"VOID":3766},"A5000598921",{"VOID":3768},"https:\u002F\u002Forcid.org\u002F0000-0002-0672-9199",{"EN":3770},"Xiujun Xie",{"id":3772,"sortIndex":19,"researcher":18,"roles":3773,"affiliations":3774,"properties":3775},"60301b85-91b6-4c3b-8c8a-c6598917b1c1",[],[],{"openalex":3776,"orcid":3778,"title":3780},{"VOID":3777},"A5063967891",{"VOID":3779},"https:\u002F\u002Forcid.org\u002F0000-0003-2413-7442",{"EN":3781},"Baoyu Zhang",{"id":3783,"sortIndex":96,"researcher":18,"roles":3784,"affiliations":3785,"properties":3786},"d22b15c0-4f4c-46a2-8e34-cb2bda86ac9f",[],[],{"openalex":3787,"orcid":3789,"title":3791},{"VOID":3788},"A5034688142",{"VOID":3790},"https:\u002F\u002Forcid.org\u002F0000-0001-9338-3001",{"EN":3792},"Huan Li",{"id":3794,"sortIndex":26,"researcher":18,"roles":3795,"affiliations":3796,"properties":3797},"1dcb0098-e9d0-4cfc-bfdd-4e647bcbb6a5",[],[],{"openalex":3798,"orcid":3800,"title":3802},{"VOID":3799},"A5000229520",{"VOID":3801},"https:\u002F\u002Forcid.org\u002F0000-0002-5582-5347",{"EN":3803},"Xueying Liu",{"url":18,"publisher":3805,"properties":3818},{"id":6,"createTime":7,"updateTime":8,"relativeEntities":3806,"slug":10,"properties":3807,"entityType":16,"verifyStatus":17,"verifyTime":18,"verifyNote":18,"syncStatus":17,"languages":18,"translateLanguages":18,"viewCount":19,"subjectFields":3810,"manageAffiliations":3811,"indexDatabases":3812,"url":23,"thumbnailPath":18,"statistic":3813,"gsStatistic":18,"type":30,"analyzePriority":18},[],{"issn":3808,"title":3809},{"VOID":13},{"EN":15},[],[],[],{"impactFactor":19,"impactFactorByYear":3814,"i10Index":19,"i10IndexLast5Year":19,"totalPublication":26,"totalPublicationByYear":3815,"totalCitation":19,"totalCitationByYear":3816,"totalCitationPerPublication":19,"totalCitationPerPublicationByYear":3817,"hindexLast5Year":19,"hindex":19},{},{"2024":26},{},{},{"volume":3819,"issue":3820},{"VOID":2091},{"VOID":2091},{"total":19,"publishYear":18,"statisticByYear":3822},{},[3824,3828,3832,3836,3840,3844,3848,3852,3856,3859,3863,3867,3871,3875,3879,3883,3887,3891,3895,3899,3903,3906,3910,3914,3918,3922,3926,3930,3934,3938,3941,3945,3949,3953,3957,3961,3964,3968,3972,3976,3980,3984,3988,3992,3996,4000,4004,4008,4012,4016,4020,4024],{"id":18,"text":3825,"url":18,"identifiers":3826},"Adler L, Díaz-Ramos A, Mao Y, Pukacz KR, Fei C, McCormick AJ. 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