Improving the sensitivity for DNA sensing based on double-anchored DNA modified gold nanoparticles

Science in China Series B: Chemistry - Tập 59 - Trang 765-769 - 2016
Xiaozhou Ma1,2,3,4,5, Miao Wang4,5,6, Chun Chen4,5, Mark Antonin Isbell4,5,7, Rui Wang1,2,3, Dongsheng Liu4,5, Zhongqiang Yang4,5
1Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University, Lanzhou, China
2State Key Laboratory of Applied Organic Chemistry and Institute of Biochemistry and Molecular Biology, Lanzhou University, Lanzhou, China
3School of Basic Medical Science, Lanzhou University, Lanzhou, China
4Key Laboratory of Organic Optoelectronics & Molecular Engineering of the Ministry of Education, Tsinghua University, Beijing, China
5Department of Chemistry, Tsinghua University, Beijing, China
6Department of Chemistry and Chemical Biology, Cornell University, Ithaca, USA
7Department of Chemical Engineering, Imperial College London, London, UK

Tóm tắt

DNA modified nanoparticles (AuNPs) are an established and widely used type of nucleotide sensor. We sought to improve the design by applying short rigid DNA duplexes near the surface of the AuNPs forming a so called double-anchored AuNP sensor, and compared it with other conventional DNA modified AuNPs. The improved design exhibited higher assembly efficiency, and consequently increased its sensitivity to target DNA.

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