Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography

Protein Expression and Purification - Tập 1 - Trang 151-154 - 1990
Jan K. Teller1, Leonard A. Fahien1
1Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, Wisconsin 53706, U.S.A.

Tài liệu tham khảo

Hansford, 1973, Active oxidative decarboxylation of malate by mitochondria isolated from L-1210 ascites tumor cells, Biochem. Biophys. Res. Commun, 51, 480, 10.1016/0006-291X(73)91282-5 Moreadith, 1984, The pathways of glutamate and glutamine oxidation by tumor cell mitochondria, J. Biol. Chem, 259, 6215, 10.1016/S0021-9258(20)82128-0 Sauer, 1980, Mitochondrial malic enzymes: Mitochondrial NAD(P)+-dependent pyruvate formation are progression-linked in Morris hepatomas, J. Biol. Chem, 255, 3844, 10.1016/S0021-9258(19)85601-6 Hsu, 1967, Pigeon liver malic enzyme: Isolation, crystallization, and some properties, J. Biol. Chem, 242, 520, 10.1016/S0021-9258(18)96304-0 Caldes, 1979, Purification of malic enzyme from bovine heart mitochondria by affinity chromatography, Anal. Biochem, 100, 299, 10.1016/0003-2697(79)90233-1 Chang, 1982, Purification of pigeon liver malic enzyme by affinity chromatography, Anal. Biochem, 121, 366, 10.1016/0003-2697(82)90494-8 Nagel, 1982, Mitochondria malic enzymes: Purification and properties of the NAD(P)+-dependent malic enzyme from canine small intestinal mucosa, J. Biol. Chem, 257, 1205, 10.1016/S0021-9258(18)33728-1 Moreadith, 1984, Purification, kinetic behavior, and regulation of NAD(P)+ malic enzyme of tumor mitochondria, J. Biol. Chem, 259, 6222, 10.1016/S0021-9258(20)82129-2 Skorkowski, 1987, Affinity chromatography on 2′,5′ ADP-Sepharose for purification of malic enzyme from crustacean muscle, J. Chromatogr, 389, 427, 10.1016/S0021-9673(01)94454-1 Sauer, 1973, Mitochondrial NAD-dependent malic enzyme: A new regulatory enzyme, FEBS Lett, 33, 251, 10.1016/0014-5793(73)80205-4 Skorkowski, 1982, Unusual behavior of NADP-linked malic enzyme from crustacean tissues on 2′,5′ ADP-Sepharose 4B, Comp. Biochem. Physiol, 728, 647 O'Carra, 1974, Lactate dehydrogenase: Specific ligand approach, Vol. 34, 598 Pedersen, 1979, Isolation of mitochondria from Morris hepatomas: Procedure using homogenization for cell disruption, Vol. 55, 84 Bradford, 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem, 72, 248, 10.1016/0003-2697(76)90527-3 Laemmli, 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227, 680, 10.1038/227680a0 Fodge, 1972, Studies on enzymes from parasitic helminths: Purification and physical properties of malic enzyme from the muscle of Ascaris suum, Biochim. Biophys. Acta, 268, 271, 10.1016/0005-2744(72)90322-1 Hsu, 1967, Pigeon liver malic enzyme: Kinetic studies, J. Biol. Chem, 242, 5315, 10.1016/S0021-9258(18)99430-5 Silverstein, 1969, Catalytic mechanism of pig heart mitochondrial malate dehydrogenase studied by kinetics at equilibrium, Biochemistry, 8, 2543, 10.1021/bi00834a042 Schimerlik, 1977, Inhibition and alternate-substrate studies on the mechanism of malic enzymes, Biochemistry, 16, 565, 10.1021/bi00623a001 Schimerlik, 1977, pH Variation of the kinetic parameters and the catalytic mechanism of malic enzyme, Biochemistry, 16, 576, 10.1021/bi00623a003