Plant growth promoting activity of an auxin and siderophore producing isolate of Streptomyces under saline soil conditions

World Journal of Microbiology and Biotechnology - Tập 28 - Trang 1503-1509 - 2011
Akram Sadeghi1, Ebrahim Karimi1, Peyman Abaszadeh Dahaji2, Majid Ghorbani Javid3, Yadola Dalvand1, Hossein Askari4
1Department of Microbial Biotechnology and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
2Department of Soil Science, College of Agriculture, University of Tehran, Karaj, Iran
3Department of Agronomy and Plant Breeding, College of Abureyhan, University of Tehran, Tehran, Iran
4Department of Biotechnology, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran

Tóm tắt

A biocontrol Streptomyces isolate (C) was tested for its plant growth promoting qualities under saline conditions. Exposure to elevated osmotic strengths up to 300 mM NaCl increased dry weight and cfu/ml significantly. The isolate C produced indolyl-3-acetic acid (IAA) into the medium in the amount of 2.4 μg/ml. The amount of auxin increased after adding salt and reached to 4.7 μg/ml in 300 mM NaCl. Biosynthesis of siderophore was detectable and increased in presence of NaCl. Streptomyces isolate C showed good solubilization of tricalcium phosphate in culture medium with 92 mg/l. Solubilization decreased in presence of NaCl. Soil treatment with isolate C increased the growth and development of wheat plant in normal and saline conditions. In this treatment there were significant increases in germination rate, percentage and uniformity, shoot length and dry weight compared to the control. Applying the bacterial inocula increased the concentration of N, P, Fe and Mn in wheat shoots grown in normal and saline soil, but had non-significant effect on other micro and macronutrients concentrations. Results of this study show that Streptomyces isolate C has potential to be utilized as biofertilizer in saline soils.

Tài liệu tham khảo

Aldesuquy HS, Mansour FA, Abo-Hamed SA (1998) Effect of the culture filtrates of Streptomyces on growth and productivity of wheat plants. Folia Microbiol 43:465–470 Alexander DB, Zuberer DA (1991) Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 12:39–45 Aly MM, El-Sabbagh SM, El-Shouny WA, Ebrahim MKH (2003) Physiological response of Zea mays to NaCl stress with respect to Azotobacter chroococcum and Streptomyces niveus. Pak J Biol Sci 6:2073–2080 Argandona M, Nieto JJ, Iglesias-Guerra F, Calderón MI, García-Estepa R, Vargas C (2010) Interplay between iron homeostasis and the osmotic stress response in the halophilic bacterium Chromohalobacter salexigens. Appl Environ Microb 76:3575–3589 Ashraf Soltani A, Khavazi K, Asadi-Rahmani H, Omidvari M, Abaszadeh Dahaji P, Mirhoseyni H (2010) Plant growth promoting characteristics in some Flavobacterium spp. isolated from soils of Iran. J Agr Sci 2:106–115 Berg G (2009) Plant–microbe interactions promoting plant growth and health: perspectives for controlled use of microorganisms in agriculture. Appl Microbiol Biot 84:11–18 Bremner JM (1965) Total nitrogen. In: Black CA (ed) Methods of soil analysis, Part 2. Chemical and microbiological properties. American Society of Agronomy, Agron. Series. No. 9, Madison, pp 1066–1083 Crowley DE, Wang YC, Reid CPP, Szaniszlo PJ (1991) Mechanisms of iron acquisition from siderophores by microorganisms and plants. Plant Soil 130:179–198 de Vasconcellos RLF, Cardoso EJBN (2009) Rhizospheric Streptomycetes as potential biocontrol agents of Fusarium and Armillaria pine rot and as PGPR for Pinus taeda. Biocontrol 54:807–816 de Weger LA, van Boxtel R, van der Burg B, Gruters RA, Geels FP, Schippers B, Lugtenberg B (1986) Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp. J Bacteriol 165:585–594 Dileep Kumar BS, Dube HC (1992) Seed bacterization with a Pseudomonas for enhanced plant growth, yield and disease control. Soil Biol Biochem 24:539–542 El-Shanshoury AR (1989) Growth promotion of wheat seedlings by Streptomyces atroolivaceus. J Agron Crop Sci 163:109–114 El-Tarabily KA (2008) Promotion of tomato (Lycopersicon esculentum Mill.) plant growth by rhizosphere competent 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomycete Actinomycetes. Plant Soil 308:161–174 Fallik E, Okon Y, Epstein E, Goldman A, Fischer M (1989) Identification and quantification of IAA and IBA in Azospirillum brasilense-inoculated maize roots. Soil Biol Biochem 21:147–153 Gupta N, Sahoo D, Bas UC (2010) Evaluation of in vitro solubilization potential of phosphate solubilising Streptomyces isolated from phyllosphere of Heritiera fomes (mangrove). Afr J Microbiol Res 4:136–142 Jeon JS, Lee SS, Kim HY, Ahn TS, Song HG (2003) Plant growth promoting in soil by some inoculated microorganism. The J Microbiol 41:271–276 M’sehli W, Jellali N, Dell’Orto M, Abdelly C, Zocchi G, Gharsalli M (2010) Responses of two lines of Medicago ciliaris to Fe deficiency under saline conditions. Plant Growth Regul doi: 10.1007/s10725-010-9561-y Macagnan D, Romeiro RS, Pomella AWV, deSouza JT (2008) Production of lytic enzymes and siderophores, and inhibition of germination of basidiospores of Moniliophthora (ex Crinipellis) perniciosa by phylloplane Actinomycetes. Biol Control 47:309–314 Mahadevan B, Crawford DL (1997) Properties of the chitinase of the antifungal biocontrol agent Streptomyces lydicus WYEC108. Enzyme Microb Tech 20:489–493 Nassar AH, El-Tarabily KA, Sivasithamparam K (2003) Growth promotion of bean (Phaseolus vulgaris L.) by a polyamine-producing isolate of Streptomyces griseoluteus. Plant Growth Regul 40:97–106 Patten C, Glick B (2002) Role of Pseudomonas putida indole acetic acid in development of the host plant root system. Appl Environ Microb 68:3795–3801 Paulitz TC, Belanger RR (2001) Biological control in greenhouce systems. Annu Rev Phytopathol 39:103–133 Prokryl Z, Vancura V, Wurst M (1985) Auxin formation by rhizosphere bacteria as a factor of root growth. Biologia Plantarum 27:159–163 Rashid M, Khalil S, Ayub N, Alam S, Latif F (2004) Organic acids productions solubilization by phosphate solubilizing microorganisms (PSM) under in vitro conditions. Pak J Biol Sci 7:187–196 Sachdev DP, Chaudhari HG, Kasture VM, Dhavale DD, Chopade BA (2009) Isolation and characterization of indole acetic acid (IAA) producing Klebsiella pneumoniae strains from rhizosphere of wheat (Triticum aestivum) and their effect on plant growth. Indian J Exp Biol 47:993–1000 Sadeghi A, Hesan AR, Askari H, Aghighi S, Shahidi Bonjar GH (2006) Biological control potential of two Streptomyces isolates on Rhizoctonia solani, the causal agent of damping-off of sugar beet. Pak J Biol Sci 9:904–910 Sadeghi A, Hesan AR, Askari H, Naderi Qomi D, Farsi M, Majidi Hervan E (2009) Biocontrol of Rhizoctonia solani damping off of sugar beet with native Streptomyces strains under field conditions. Biocontrol Sci Techn 19:985–991 Saleem M, Arshad M, Hussain S, Bhatti AS (2007) Perspective of plant growth promoting rhizobacteria (PGPR) containing ACC deaminase in stress agriculture. J Ind Microbiol Biot 34:635–648 Shahidi Bonjar GH, Rashid Farrokhi P, Aghighi S, Shahidi Bonjar L, Aghelizadeh A (2005) Antifungal characterization of actinomycetes isolated from Kerman, Iran and their future prospects in biological control strategies in greenhouse and field conditions. Plant Pathol J 4:78–84 Tank N, Saraf M (2010) Salinity-resistant plant growth promoting rhizobacteria ameliorates sodium chloride stress on tomato plants. J Plant Interact 5:51–58 Tokala RK, Strap JL, Jung CM, Crawford DL, Salove MH, Deobald LA, Bailey JF, Morra MJ (2002) Novel plant-microbe rhizosphere interaction involving Streptomyces lydicus WYEC108 and the pea plant (Pisum sativum). Appl Environ Microb 68:2161–2171 Trejo-Estrada SR, Paszczynski A, Crawford DL (1998) Antibiotics and enzymes produced by the biocontrol agent Streptomyces violaceusniger YCED-9. J Ind Microbiol Biot 21:81–90 Tsavkelova EA, Cherdyntseva TA, Klimova SY, Shestakov AI, Botina SG, Netrusov AI (2007) Orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin. Arch Microbiol 188:655–664 Vivas A, Marulanda A, Ruiz-Lozano JM, Barea JM, Azcon R (2003) Influence of a Bacillus sp. on physiological activities of two arbuscular mycorrhizal fungi and on plant responses to PEG induced drought stress. Mycorrhiza 13:249–256 Waksman SA (1959) The Streptomyces, vol. 1. Williams and Wilkins Co., Baltimore Wang Y, Brown HN, Crowley DE, Szaniszlo PJ (1993) Evidence for direct utilization of a siderophore, ferroxamine B, in axenically grown cucumber. Plant Cell Environ 16:579–585 Whipps J (2001) Microbial interactions and biocontrol in the rhizosphere. J Exp Bot 52:487–511 Zahran HH (1997) Diversity, adaptation and activity of the bacterial flora in saline environments. Biol Fertil Soils 25:211–223